Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 264,164 C→T 5.9% F305F (TTC→TTT)  gabD‑I → succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947264,1640CT 5.9% 583.7 / 17.0 188F305F (TTC→TTT) gabD‑Isuccinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (86/90);  new base T (5/6);  total (92/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 194 total aligned reads displayed.

CTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCT  >  NC_002947/264015‑264313
                                                                                                                                                     |                                                                                                                                                     
  gggTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGTCGCGTTCGc                                                                                                                                                     >  2:3196163/1‑150 (MQ=255)
   ggcggCATCGCTCCTTTCATCGTGTTCGACTACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACCACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGcc                                                                                                                                                    <  2:2693764/147‑1 (MQ=255)
   ggTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGcc                                                                                                                                                    >  2:3426201/1‑150 (MQ=255)
       gCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCgaga                                                                                                                                                >  2:2349931/1‑150 (MQ=255)
       gCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCgaga                                                                                                                                                >  1:2286302/1‑150 (MQ=255)
        cAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGaa                                                                                                                                               >  1:1152410/1‑150 (MQ=255)
        cAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCATTGCCGAGaa                                                                                                                                               <  2:4725041/150‑1 (MQ=255)
            gcgcctttCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCtc                                                                                                                                           <  1:5152214/144‑2 (MQ=255)
            gCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTg                                                                                                                                           <  1:449123/150‑1 (MQ=255)
            gCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTg                                                                                                                                           <  1:1238558/150‑1 (MQ=255)
            gCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTg                                                                                                                                           >  1:5159840/1‑150 (MQ=255)
             cgcctttCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCtct                                                                                                                                          <  1:3508143/145‑3 (MQ=255)
               ccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGcc                                                                                                                                        >  2:3521307/1‑150 (MQ=255)
                    cATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGc                                                                                                                                   >  2:185900/1‑150 (MQ=255)
                     aTCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCa                                                                                                                                  <  2:5159840/150‑1 (MQ=255)
                     aTCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCa                                                                                                                                  <  2:5392053/150‑1 (MQ=255)
                          gtTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGc                                                                                                                             >  2:3427562/1‑150 (MQ=255)
                           ttCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGc                                                                                                                                             >  2:4216746/1‑133 (MQ=255)
                             cgacgaCGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCaa                                                                                                                          <  2:2261179/150‑1 (MQ=255)
                               acgacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGc                                                                                                                        >  1:1731880/1‑150 (MQ=255)
                                 gacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACATCGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTg                                                                                                                      >  2:1339880/1‑150 (MQ=255)
                                 gacgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTg                                                                                                                      >  2:1024/1‑150 (MQ=255)
                                  acgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGa                                                                                                                     <  1:4319564/150‑1 (MQ=255)
                                  acgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGa                                                                                                                     <  1:3426201/150‑1 (MQ=255)
                                   cgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGaa                                                                                                                    <  2:2920084/150‑1 (MQ=255)
                                   cgCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGaa                                                                                                                    >  2:618293/1‑150 (MQ=255)
                                    gCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAg                                                                                                                   >  1:5400562/1‑150 (MQ=255)
                                       gACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATc                                                                                                                <  1:4742049/150‑1 (MQ=255)
                                          cTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGt                                                                                                             >  2:2000463/1‑150 (MQ=255)
                                                  ggCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCt                                                                                                     <  2:3921996/150‑1 (MQ=255)
                                                     ggTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGga                                                                                                  <  1:2094857/150‑1 (MQ=255)
                                                      gTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaa                                                                                                 <  2:1090239/150‑1 (MQ=255)
                                                      gTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaa                                                                                                 <  2:4347433/150‑1 (MQ=255)
                                                         gAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaagaa                                                                                              <  1:4089199/150‑1 (MQ=255)
                                                         gAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGgaagaa                                                                                              <  1:2000463/150‑1 (MQ=255)
                                                              cgcgATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcac                                                                                         <  2:943371/150‑1 (MQ=255)
                                                               gcgATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcacc                                                                                        <  1:5275930/150‑1 (MQ=255)
                                                               gcgATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcacc                                                                                        <  1:4948851/150‑1 (MQ=255)
                                                                 gATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcaccac                                                                                      >  1:893669/1‑150 (MQ=255)
                                                                      tctcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                               <  1:4936304/120‑1 (MQ=255)
                                                                      tctcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg                                                                                                               >  2:4936304/1‑120 (MQ=255)
                                                                      tctcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTg                                                                                 >  1:4395664/1‑150 (MQ=255)
                                                                      tctcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTg                                                                                 <  1:2977812/150‑1 (MQ=255)
                                                                       ctcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTgg                                                                                <  2:3823558/150‑1 (MQ=255)
                                                                        tcCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGc                                                                               <  2:1279991/150‑1 (MQ=255)
                                                                         ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcacca                                                                                       >  1:4862627/1‑141 (MQ=255)
                                                                         ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGcacca                                                                                       <  2:4862627/141‑1 (MQ=255)
                                                                         ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGcc                                                                              <  1:2349931/150‑1 (MQ=255)
                                                                          cAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGccc                                                                             >  1:1337453/1‑150 (MQ=255)
                                                                                ccGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGAt                                                                       <  1:186261/150‑1 (MQ=255)
                                                                                ccGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGAt                                                                       >  2:2806268/1‑150 (MQ=255)
                                                                                 cgcaacaacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATc                                                                      >  1:3253440/4‑150 (MQ=255)
                                                                                 cgcaacaacGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATc                                                                      >  2:1319927/4‑150 (MQ=255)
                                                                                 cGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATc                                                                      >  2:533068/1‑150 (MQ=255)
                                                                                  gTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCg                                                                     >  1:349964/1‑150 (MQ=255)
                                                                                   tAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGa                                                                    <  2:4064585/150‑1 (MQ=255)
                                                                                   tAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGa                                                                    >  2:1390125/1‑150 (MQ=255)
                                                                                    aacaacGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGAt                                                                   <  1:1024/150‑1 (MQ=255)
                                                                                       aacGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGc                                                                >  2:3618814/1‑150 (MQ=255)
                                                                                       aacGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGc                                                                >  2:1132529/1‑150 (MQ=255)
                                                                                          ggCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAg                                                             <  1:700373/150‑1 (MQ=255)
                                                                                           gCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAgg                                                            <  1:38943/150‑1 (MQ=255)
                                                                                              aaaCCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGAt                                                                   >  1:1728743/3‑140 (MQ=255)
                                                                                              aaaCCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCTCTCCAAGGCGGTCGGCTCGCTCAAGACCGGCAACGGCTTCGACGAGGGCATCAATCTCGGCCCGCTGATCGAt                                                                   <  2:1728743/138‑1 (MQ=255)
                                                                                                aCCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTc                                                       <  1:100638/150‑1 (MQ=255)
                                                                                                           cgcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCa                                            <  1:3581861/150‑1 (MQ=255)
                                                                                                            gcCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAg                                           >  2:4588060/1‑150 (MQ=255)
                                                                                                             ccAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATg                                                                  <  1:2536111/126‑1 (MQ=255)
                                                                                                             ccAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATg                                                                  >  2:2536111/1‑126 (MQ=255)
                                                                                                             ccAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAgg                                          >  1:4616075/1‑150 (MQ=255)
                                                                                                               aaCCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGcc                                                                                                                                        >  1:4281649/1‑54 (MQ=255)
                                                                                                               aaCCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGcc                                                                                                                                        <  2:4281649/54‑1 (MQ=255)
                                                                                                                 ccGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAaca                                      >  1:2705261/1‑150 (MQ=255)
                                                                                                                 ccGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAaca                                      >  1:355824/1‑150 (MQ=255)
                                                                                                                    tATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACAt                                   >  2:4680585/1‑150 (MQ=255)
                                                                                                                     aTCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATc                                  >  2:2240577/1‑150 (MQ=255)
                                                                                                                       cTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGa                                >  2:166047/1‑150 (MQ=255)
                                                                                                                        tACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAg                               >  1:2427683/1‑150 (MQ=255)
                                                                                                                             gCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAg                                                 <  1:767122/127‑1 (MQ=255)
                                                                                                                             gCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAg                                                 >  2:767122/1‑127 (MQ=255)
                                                                                                                              cAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGcc                         >  1:3120661/1‑150 (MQ=255)
                                                                                                                              cAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGcc                         <  1:5397655/150‑1 (MQ=255)
                                                                                                                                ggACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTg                                                         <  2:3343894/116‑1 (MQ=255)
                                                                                                                                ggACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTg                                                         >  1:3343894/1‑116 (MQ=255)
                                                                                                                                  aCGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCa                     <  1:2265247/150‑1 (MQ=255)
                                                                                                                                  aCGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCa                     <  1:3884643/150‑1 (MQ=255)
                                                                                                                                  aCGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCa                     >  2:4776355/1‑150 (MQ=255)
                                                                                                                                  aCGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCa                     <  2:2365814/150‑1 (MQ=255)
                                                                                                                                   cGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGGAAGGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAg                    >  2:1635883/1‑150 (MQ=255)
                                                                                                                                       gTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCaaa                >  1:2315202/1‑150 (MQ=255)
                                                                                                                                       gTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCaaa                >  1:4385816/1‑150 (MQ=255)
                                                                                                                                       gTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCaaa                <  2:835213/150‑1 (MQ=255)
                                                                                                                                          tACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCaa                                                  <  1:1425768/113‑1 (MQ=255)
                                                                                                                                          tACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCaa                                                  >  2:1425768/1‑113 (MQ=255)
                                                                                                                                             gacgCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAg                                                             >  1:1087319/1‑99 (MQ=255)
                                                                                                                                             gacgCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGcc          >  1:1383427/1‑150 (MQ=255)
                                                                                                                                                gcgTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCGGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCACAGGCGCCaaa       >  1:1042868/1‑150 (MQ=255)
                                                                                                                                                  gTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACg                           <  2:3540964/128‑1 (MQ=255)
                                                                                                                                                     cGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGTTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCt  <  2:2286302/150‑1 (MQ=255)
                                                                                                                                                     cGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCt  <  2:1757191/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAGGCTGTCGCCAAGGTCCAGGAACACATCGAGGACGCCGTCAGCAAAGGCGCCAAAGTGCT  >  NC_002947/264015‑264313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑