Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,675,517 G→C 8.5% P107P (CCG→CCC)  PP_4137 → outer membrane siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,675,5170GC 8.5% 568.5 / 33.4 176P107P (CCG→CCC) PP_4137outer membrane siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (83/78);  new base C (6/9);  total (89/87)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 186 total aligned reads displayed.

AGCAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTGG  >  NC_002947/4675368‑4675665
                                                                                                                                                     |                                                                                                                                                    
 gCAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCgg                                                                                                                                                     <  2:4450829/150‑1 (MQ=255)
  cAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGTCGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCggg                                                                                                                                                    <  2:2830505/150‑1 (MQ=255)
   aaCCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCgggg                                                                                                                                                   >  2:2880791/1‑150 (MQ=255)
    aCCTGGTTGACATGCAGCGCGTGGGGCAAGGCCGTGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCggggg                                                                                                                                                  <  1:3417691/150‑1 (MQ=255)
       tggtggACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGgcgc                                                                                                                                               >  1:5298552/1‑150 (MQ=255)
           ggACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACgg                                                                                                                                           <  1:2601853/150‑1 (MQ=255)
             aCATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGtt                                                                                                                                         <  1:3530599/150‑1 (MQ=255)
             aCATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACGGtt                                                                                                                                         <  2:1851920/150‑1 (MQ=255)
             aCATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACGGtt                                                                                                                                         <  2:1852010/150‑1 (MQ=255)
                  cAGCGCGTGGGGCACGGCCGAGTGGCCAGCAACCTGGACGTGCTGGCCTACCAGCCTGGGGTGTTCGCCAAATGGGCTGTTAACGTTGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGgt                                                                                                                                    <  1:4733215/150‑1 (MQ=255)
                  cAGCGCGTGGGGCAAGGCCGCGGGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGgt                                                                                                                                    <  2:3580287/150‑1 (MQ=255)
                    gcgcGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCTGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGGGCGCCGGGGGCGCACGGTTCCGgtgt                                                                                                                                  <  2:2837057/150‑1 (MQ=255)
                      gcgTGTGGCCAGGCCGATAGGCCAGCAACCAGCACGTGCTGGCCTACCAGCCCGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGCGCCTCAAACGTGCGCCGGGGGCGCACGGAACCGGTGTCt                                                                                                                                >  2:3186528/1‑150 (MQ=255)
                              cAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATg                                                                                                                        >  1:4846466/1‑150 (MQ=255)
                              cAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACGGttgag                                                                                                                                      >  2:382544/1‑133 (MQ=255)
                               aaGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGt                                                                                                                       <  2:4012086/150‑1 (MQ=255)
                                  gCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCg                                                                                                                    >  2:4665272/1‑150 (MQ=255)
                                    cgcggggCCAGCACCCCGGAGGTGCGGGCCTACCCGCCTGGGGGGTGCGCCCAAGGGGCTGGTACCGATGGCATCACGCTGTCGATCCGCGGCGGGGGCCTCACCCGTGCGCGGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGAc                                                                                                                  <  1:2411598/145‑1 (MQ=255)
                                          gCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATATGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTg                                                                                                            <  1:5356426/150‑1 (MQ=255)
                                          gCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTg                                                                                                            >  2:287470/1‑150 (MQ=255)
                                             aGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCg                                                                                                         >  2:2691724/1‑150 (MQ=255)
                                               cAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGtt                                                                                                       <  2:3499839/150‑1 (MQ=255)
                                                 aCCAGGACGTGCTGGCCTACCAGCCTGGGGTGTTCGCCCAATCGGCAGTTAACGATGGAATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGa                                                                                                     <  1:2011240/150‑1 (MQ=255)
                                                  ccAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTacac                                                                                                                            <  2:3626419/126‑1 (MQ=255)
                                                  ccAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTacac                                                                                                                            >  1:3626419/1‑126 (MQ=255)
                                                   cAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGAcc                                                                                                   >  2:2334517/1‑150 (MQ=255)
                                                   cAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGGTCAACGGCAGGCCGTCGaac                                                                                                   <  2:4268175/150‑3 (MQ=255)
                                                    aGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCg                                                                                                  >  1:4690126/1‑150 (MQ=255)
                                                     ggACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGgcg                                                                                                                                                >  1:982263/1‑103 (MQ=255)
                                                     ggACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTac                                                                                                                              <  2:5101731/121‑1 (MQ=255)
                                                             tGGCCTACCAGTCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGttgagg                                                                                                                                     >  1:5097038/1‑102 (MQ=255)
                                                             tGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGggcg                                                                                         >  2:3387126/1‑150 (MQ=255)
                                                               gCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGggcggc                                                                                       >  1:4947703/1‑150 (MQ=255)
                                                                  tACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACg                                                                                    >  2:2002254/1‑150 (MQ=255)
                                                                  tACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTAAAACATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACg                                                                                    >  1:1829409/1‑150 (MQ=255)
                                                                   aCCAGCCTGGGGTGTTCGCCAAATCGGCCGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCACCCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGc                                                                                   <  1:219253/150‑1 (MQ=255)
                                                                   aCCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCATCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGc                                                                                   >  1:2811994/1‑150 (MQ=255)
                                                                      agccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCtt                                                                                >  2:651017/1‑150 (MQ=255)
                                                                       gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTa                                                                               >  2:4131516/1‑150 (MQ=255)
                                                                       gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTa                                                                               <  2:1188787/150‑1 (MQ=255)
                                                                       gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTa                                                                               <  2:1188695/150‑1 (MQ=255)
                                                                       gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGCCACGCCTTa                                                                               <  2:1307474/150‑1 (MQ=255)
                                                                           ggggTGTTCGCCCAATCGGCAGTTAACGATGGCATCAAGCTTTCGATACGCGGCTCGGGCCTAAACCGTGCGCCGGGGGCGCACGGTTCCTGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                           <  2:3205031/150‑1 (MQ=255)
                                                                           ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCATGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                           <  2:92632/150‑1 (MQ=255)
                                                                           ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                           <  2:4719521/150‑1 (MQ=255)
                                                                           ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                           >  1:1516568/1‑150 (MQ=255)
                                                                           ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                           >  1:4262679/1‑150 (MQ=255)
                                                                           ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCGCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                           <  2:5167669/150‑1 (MQ=255)
                                                                              gtgtTCGCCCAATCGGCAGGTAACGATGGCATCACGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTg                                                                        <  1:701918/150‑1 (MQ=255)
                                                                                  tCGCCAAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCg                                                                    <  1:1097315/150‑1 (MQ=255)
                                                                                    gCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGaa                                                                  >  2:4068710/1‑150 (MQ=255)
                                                                                     cccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAc                                                                 >  1:3982714/1‑150 (MQ=255)
                                                                                      ccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAcc                                                                >  2:1434935/1‑150 (MQ=255)
                                                                                      ccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAcc                                                                >  1:3882532/1‑150 (MQ=255)
                                                                                      caaaTCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAcc                                                                <  2:499595/148‑1 (MQ=255)
                                                                                           cGGCTGGTAACGATGGCATCAAGGTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCGGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                           <  1:639965/150‑1 (MQ=255)
                                                                                           cGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                           <  1:4839304/150‑1 (MQ=255)
                                                                                               aGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCt                                                       >  1:2984417/1‑150 (MQ=255)
                                                                                                 gTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGa                                                     >  2:1667049/1‑150 (MQ=255)
                                                                                                 gTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGa                                                     <  1:2017876/150‑1 (MQ=255)
                                                                                                  tAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCGGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAg                                                    <  1:726263/150‑1 (MQ=255)
                                                                                                  tAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCCTGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAg                                                    >  2:2752657/1‑150 (MQ=255)
                                                                                                    aCGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGc                                                   <  2:3339074/149‑1 (MQ=255)
                                                                                                    aCGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGc                                                   >  1:3339074/1‑149 (MQ=255)
                                                                                                        tGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGc                                              >  2:2469954/1‑150 (MQ=255)
                                                                                                            aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCAt                                                                                                                         <  2:5409547/71‑1 (MQ=255)
                                                                                                            aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                          >  2:300526/1‑150 (MQ=255)
                                                                                                            aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                          >  2:3897844/1‑150 (MQ=255)
                                                                                                            aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                          <  1:2384510/150‑1 (MQ=255)
                                                                                                            aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                          >  1:5109565/1‑150 (MQ=255)
                                                                                                            aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                          <  2:3234789/150‑1 (MQ=255)
                                                                                                             tCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCGGTGGCTGAGCCGGGcctgaa                                         <  2:4964640/150‑5 (MQ=255)
                                                                                                              cAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGt                                        <  2:752600/150‑1 (MQ=255)
                                                                                                                   tGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGtgctgc                                   >  2:2075468/1‑150 (MQ=255)
                                                                                                                     tCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGt                                 >  2:4369119/1‑150 (MQ=255)
                                                                                                                       gATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCTGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTgg                               >  2:3598950/1‑150 (MQ=255)
                                                                                                                       gATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTgg                               >  2:4200223/1‑150 (MQ=255)
                                                                                                                              ggCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAAt                        <  1:1126025/150‑1 (MQ=255)
                                                                                                                                 tCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGc                     >  2:122782/1‑150 (MQ=255)
                                                                                                                                 tCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGc                     >  2:1114066/1‑150 (MQ=255)
                                                                                                                                  cGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCt                    <  1:4893419/150‑1 (MQ=255)
                                                                                                                                   gggCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCaggttgct                   <  2:2388867/150‑8 (MQ=255)
                                                                                                                                    ggCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                  <  1:2334517/150‑1 (MQ=255)
                                                                                                                                    ggCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                  >  1:2172783/1‑150 (MQ=255)
                                                                                                                                    ggCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                  <  1:3000292/150‑1 (MQ=255)
                                                                                                                                    ggCATCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGa                                                                                                     <  1:4534172/67‑1 (MQ=255)
                                                                                                                                     accggaaCCGTGCGCCCCCGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTg                 >  1:4070415/6‑150 (MQ=255)
                                                                                                                                       cggaaCCGTGCGCCCCCGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgtg                                                          <  1:1745447/104‑1 (MQ=39)
                                                                                                                                       cTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAc               >  1:4410287/1‑150 (MQ=255)
                                                                                                                                       cTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAc               <  1:1653570/150‑1 (MQ=255)
                                                                                                                                        tCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                           >  1:3230289/1‑105 (MQ=255)
                                                                                                                                        tCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAcc              >  1:2048697/1‑150 (MQ=255)
                                                                                                                                          aaCCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCaa            >  2:4595663/1‑150 (MQ=255)
                                                                                                                                          aaCCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCaa            >  2:1815981/1‑150 (MQ=255)
                                                                                                                                             cGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGc         >  1:4989543/1‑150 (MQ=255)
                                                                                                                                             cGTGCGCCCCCGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                  >  1:4829369/1‑141 (MQ=255)
                                                                                                                                              gTGCGCCCCCGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGGGGCGCCAATGGCGTTGACCAAGGCt        >  1:2511454/1‑150 (MQ=255)
                                                                                                                                                gcgcCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTcc      >  1:764038/1‑150 (MQ=255)
                                                                                                                                                gcgcCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTcc      >  1:4062115/1‑150 (MQ=255)
                                                                                                                                                   ccGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTg   >  1:1358287/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTGG  >  NC_002947/4675368‑4675665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑