Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,675,518 G→C 8.5% G108R (GGG→CGG) ‡ PP_4137 → outer membrane siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,675,5180GC 8.5% 568.2 / 33.2 176G108R (GGG→CGG) ‡PP_4137outer membrane siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (83/78);  new base C (6/9);  total (89/87)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 185 total aligned reads displayed.

GCAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTGGCC  >  NC_002947/4675369‑4675667
                                                                                                                                                     |                                                                                                                                                     
gCAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCgg                                                                                                                                                       <  2:4450829/150‑1 (MQ=255)
 cAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGTCGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCggg                                                                                                                                                      <  2:2830505/150‑1 (MQ=255)
 cAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCggg                                                                                                                                                      <  2:2830648/150‑1 (MQ=255)
  aaCCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCgggg                                                                                                                                                     >  2:2880787/1‑150 (MQ=255)
        gtggACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCAc                                                                                                                                               >  1:4067951/1‑150 (MQ=255)
            aCATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACGGtt                                                                                                                                           <  1:4793896/150‑1 (MQ=255)
            aCATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACGGtt                                                                                                                                           <  2:1852010/150‑1 (MQ=255)
               tGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCACGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCg                                                                                                                                        <  2:3874382/150‑1 (MQ=255)
                 cAGCGCGTGGGGCACGGCCGAGTGGCCAGCAACCTGGACGTGCTGGCCTACCAGCCTGGGGTGTTCGCCAAATGGGCTGTTAACGTTGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGgt                                                                                                                                      <  1:4733215/150‑1 (MQ=255)
                 cAGCGCGTGGGGCAAGGCCGCGGGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGgt                                                                                                                                      <  2:3580287/150‑1 (MQ=255)
                 cAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAAACGCCAGGTAACGATGGAATCAAGCTGTCGATACGCGGATCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGgt                                                                                                                                      <  1:4834264/150‑1 (MQ=255)
                     gcgTGTGGCCAGGCCGATAGGCCAGCAACCAGCACGTGCTGGCCTACCAGCCCGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGCGCCTCAAACGTGCGCCGGGGGCGCACGGAACCGGTGTCt                                                                                                                                  >  2:3186528/1‑150 (MQ=255)
                         ggggCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTacac                                                                                                                              <  2:133366/150‑1 (MQ=255)
                            gCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCAt                                                                                                                           >  1:754014/1‑150 (MQ=255)
                             cAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATg                                                                                                                          >  1:4846466/1‑150 (MQ=255)
                              aaGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGt                                                                                                                         <  2:4012086/150‑1 (MQ=255)
                                 gCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCg                                                                                                                      >  2:4665272/1‑150 (MQ=255)
                                   cgcggggCCAGCACCCCGGAGGTGCGGGCCTACCCGCCTGGGGGGTGCGCCCAAGGGGCTGGTACCGATGGCATCACGCTGTCGATCCGCGGCGGGGGCCTCACCCGTGCGCGGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGAc                                                                                                                    <  1:2411598/145‑1 (MQ=255)
                                    gAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACg                                                                                                                   <  2:3787715/150‑1 (MQ=255)
                                         gCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATATGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTg                                                                                                              <  1:5356426/150‑1 (MQ=255)
                                          ccAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGc                                                                                                             >  2:1131257/1‑150 (MQ=255)
                                            aGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCg                                                                                                           >  2:3288115/1‑150 (MQ=255)
                                             gCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGt                                                                                                          >  2:1521331/1‑150 (MQ=255)
                                              cAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGtt                                                                                                         <  1:3288115/150‑1 (MQ=255)
                                              cAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGtt                                                                                                         <  2:3499839/150‑1 (MQ=255)
                                                 ccAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTacac                                                                                                                              >  1:3626419/1‑126 (MQ=255)
                                                 ccAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCAACGGCAGGCCGTCGaa                                                                                                      <  2:2413647/150‑2 (MQ=255)
                                                   aGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCg                                                                                                    >  1:4690126/1‑150 (MQ=255)
                                                    ggACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTac                                                                                                                                >  1:5101731/1‑121 (MQ=255)
                                                    ggACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTac                                                                                                                                <  2:5101731/121‑1 (MQ=255)
                                                            tGGCCTACCAGTCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGttgagg                                                                                                                                       >  1:5097038/1‑102 (MQ=255)
                                                            tGGCCTACCAGTCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGttgagg                                                                                                                                       <  2:5097038/106‑5 (MQ=255)
                                                            tGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGggcg                                                                                           >  2:3387126/1‑150 (MQ=255)
                                                              gCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGggcggc                                                                                         >  1:4947703/1‑150 (MQ=255)
                                                                 tACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACg                                                                                      >  1:2995034/1‑150 (MQ=255)
                                                                 tACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACg                                                                                      >  2:2002254/1‑150 (MQ=255)
                                                                 tACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTAAAACATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACg                                                                                      >  1:1829409/1‑150 (MQ=255)
                                                                  aCCAGCCTGGGGTGTTCGCCAAATCGGCCGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCACCCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGc                                                                                     <  1:219253/150‑1 (MQ=255)
                                                                  aCCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCACGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGc                                                                                     <  1:5141660/150‑1 (MQ=255)
                                                                     agccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCtt                                                                                  >  2:651017/1‑150 (MQ=255)
                                                                      gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTa                                                                                 >  2:4131516/1‑150 (MQ=255)
                                                                      gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTa                                                                                 <  2:1188787/150‑1 (MQ=255)
                                                                      gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGCCACGCCTTa                                                                                 <  2:1307474/150‑1 (MQ=255)
                                                                          ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCATGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                             <  2:92632/150‑1 (MQ=255)
                                                                          ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                             >  1:1516568/1‑150 (MQ=255)
                                                                          ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                             <  2:4719521/150‑1 (MQ=255)
                                                                          ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                             >  1:4262679/1‑150 (MQ=255)
                                                                          ggggTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCGCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                             <  2:5167669/150‑1 (MQ=255)
                                                                             gtgtTCGCCCAATCGGCAGGTAACGATGGCATCACGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTg                                                                          <  1:701918/150‑1 (MQ=255)
                                                                               gtTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGGCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGtt                                                                        <  2:1844026/150‑1 (MQ=255)
                                                                                 tCGCCAAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCg                                                                      <  1:1097315/150‑1 (MQ=255)
                                                                                  cGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGa                                                                     <  2:2315913/150‑1 (MQ=255)
                                                                                   gCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGaa                                                                    >  2:4068710/1‑150 (MQ=255)
                                                                                    cccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAc                                                                   >  1:3982738/1‑150 (MQ=255)
                                                                                    cccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAc                                                                   >  1:3982714/1‑150 (MQ=255)
                                                                                     ccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAcc                                                                  >  2:2347159/1‑150 (MQ=255)
                                                                                     ccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAcc                                                                  >  1:3882532/1‑150 (MQ=255)
                                                                                          cGGCTGGTAACGATGGCATCAAGGTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCGGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                             <  1:639965/150‑1 (MQ=255)
                                                                                           ggCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgtg                                                            >  1:79702/1‑150 (MQ=255)
                                                                                              aGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCt                                                         >  1:253787/1‑150 (MQ=255)
                                                                                                gTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGa                                                       <  1:2017876/150‑1 (MQ=255)
                                                                                                gTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGa                                                       <  1:2800153/150‑1 (MQ=255)
                                                                                                 tAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCGGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAg                                                      <  1:726263/150‑1 (MQ=255)
                                                                                                 tAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCCTGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAg                                                      >  2:2752657/1‑150 (MQ=255)
                                                                                                 tAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAg                                                      >  2:2982146/1‑150 (MQ=255)
                                                                                                   aCGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGcc                                                    <  1:3091343/150‑1 (MQ=255)
                                                                                                   aCGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGc                                                     <  2:3339074/149‑1 (MQ=255)
                                                                                                   aCGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGc                                                     >  1:3339074/1‑149 (MQ=255)
                                                                                                     gATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCGTACGAACTGTTCGAACCGCTGTGGCTGAGCCGt                                                  >  1:1841453/1‑150 (MQ=255)
                                                                                                      aTGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTg                                                 >  1:3544289/1‑150 (MQ=255)
                                                                                                           aTCAAGCTGTCGATACGCGGCTCGGGCCTCACCCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                            <  1:3490667/150‑1 (MQ=255)
                                                                                                           aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCAt                                                                                                                           <  2:5409547/71‑1 (MQ=255)
                                                                                                           aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                            <  1:2384510/150‑1 (MQ=255)
                                                                                                           aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGaa                                            >  2:3897844/1‑150 (MQ=255)
                                                                                                            tCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCGGTGGCTGAGCCGGGcctgaa                                           <  2:4964640/150‑5 (MQ=255)
                                                                                                             cAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGt                                          <  2:752600/150‑1 (MQ=255)
                                                                                                                  tGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGtgctgc                                     >  2:2075468/1‑150 (MQ=255)
                                                                                                                    tCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGt                                   >  2:4369119/1‑150 (MQ=255)
                                                                                                                      gATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCTGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTgg                                 >  2:3598950/1‑150 (MQ=255)
                                                                                                                      gATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTgg                                 >  2:4200223/1‑150 (MQ=255)
                                                                                                                             ggCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                             <  2:2856478/115‑1 (MQ=255)
                                                                                                                             ggCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                             >  1:2856478/1‑115 (MQ=255)
                                                                                                                             ggCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAAt                          <  1:1126025/150‑1 (MQ=255)
                                                                                                                                tCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGACCTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAGGTGCTGCGTGGCGCCAATGGc                       >  2:1113864/1‑150 (MQ=255)
                                                                                                                                tCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGc                       >  2:122782/1‑150 (MQ=255)
                                                                                                                                 cGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCt                      <  1:4893419/150‑1 (MQ=255)
                                                                                                                                   ggCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                    >  1:2172783/1‑150 (MQ=255)
                                                                                                                                   ggCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                    <  1:2334517/150‑1 (MQ=255)
                                                                                                                                   ggCATCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGa                                                                                                       <  1:4534172/67‑1 (MQ=255)
                                                                                                                                     ccTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGtt                                                                        >  2:4308168/1‑96 (MQ=255)
                                                                                                                                      cggaaCCGTGCGCCCCCGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgtg                                                            <  1:1745447/104‑1 (MQ=39)
                                                                                                                                      cggaaCCGTGCGCCCCCGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgtg                                                            >  2:1745447/4‑107 (MQ=39)
                                                                                                                                      cTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAc                 <  1:1653570/150‑1 (MQ=255)
                                                                                                                                      cTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAc                 >  1:4410287/1‑150 (MQ=255)
                                                                                                                                       tCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                             <  2:3230289/105‑1 (MQ=255)
                                                                                                                                       tCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAcc                >  1:2048697/1‑150 (MQ=255)
                                                                                                                                         aaCCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCaa              >  2:4595663/1‑150 (MQ=255)
                                                                                                                                         aaCCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCaa              <  2:2471296/150‑1 (MQ=255)
                                                                                                                                            cGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGc           >  1:4989543/1‑150 (MQ=255)
                                                                                                                                                  ccGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTg     >  1:1358287/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTGGCC  >  NC_002947/4675369‑4675667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑