BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	311,682	G→A	8.4%	P232P (CCC→CCT)	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	311,682	0	G	A	8.4%	625.0 / 33.4	213	P232P (CCC→CCT)	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (100/95); new base A (4/14); total (104/109)
Fisher's exact test for biased strand distribution p-value = 2.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 217 total aligned reads displayed.

GGTCACTGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGT  >  NC_002947/311534‑311830

 gTCACTGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCggg                                                                                                                                                     <  2:3597903/150‑1 (MQ=255)

  tCACTGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCgggg                                                                                                                                                    >  1:1494475/1‑150 (MQ=255)

   cACTGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGAGGCACACCCCCTGGGCGCCACGCTTGACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGGGCCGGGGt                                                                                                                                                   <  2:5049246/150‑1 (MQ=255)

     cTGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTc                                                                                                                                                 <  2:2681089/150‑1 (MQ=255)

      tGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCt                                                                                                                                                >  1:1102996/1‑150 (MQ=255)

      tGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCt                                                                                                                                                >  2:651250/1‑150 (MQ=255)

      tGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCt                                                                                                                                                >  2:5111275/1‑150 (MQ=255)

      tGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCt                                                                                                                                                >  2:2721429/1‑150 (MQ=255)

      tGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGATCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCt                                                                                                                                                >  1:523996/1‑150 (MQ=255)

        ttGGTCATCATTTCCTGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTTCCGGGGTTCTgg                                                                                                                                              <  2:1741416/150‑1 (MQ=255)

        ttGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTgg                                                                                                                                              >  1:1253486/1‑150 (MQ=255)

        ttGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTgg                                                                                                                                              >  2:541350/1‑150 (MQ=255)

          ggTCAGCATTTCCAGTGGGTTCTGTGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGcc                                                                                                                                            <  2:360334/150‑1 (MQ=255)

           gTCAGCATTTGCTGTGGGTTCTGGGGGTGGTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGTGTGATTGGTGCCGGGGTTCTGGccc                                                                                                                                           <  2:2544263/150‑1 (MQ=255)

             cAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCa                                                                                                                                         >  2:2220821/1‑150 (MQ=255)

             cAGCATTTCCAGTGGGGTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCa                                                                                                                                         <  2:1314455/150‑1 (MQ=255)

               gCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGa                                                                                                                                       >  2:3196537/1‑150 (MQ=255)

               gCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGa                                                                                                                                       <  1:3602966/150‑1 (MQ=255)

               gCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGa                                                                                                                                       <  2:2635054/150‑1 (MQ=255)

               gCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGa                                                                                                                                       >  2:3511294/1‑150 (MQ=255)

                   ttCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACaaa                                                                                                                                   >  1:524788/1‑150 (MQ=255)

                     ccAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACaaaaa                                                                                                                                 <  1:4456320/150‑1 (MQ=255)

                      cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                <  1:4491650/150‑1 (MQ=255)

                      cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                >  1:4259695/1‑150 (MQ=255)

                      cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                <  2:3355823/150‑1 (MQ=255)

                      cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                >  2:3451392/1‑150 (MQ=255)

                      cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                >  1:5335527/1‑150 (MQ=255)

                      cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                >  2:1826932/1‑150 (MQ=255)

                           ggTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGt                                                                                                                           <  2:1431704/150‑1 (MQ=255)

                            gTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTc                                                                                                                          <  2:524788/150‑1 (MQ=255)

                               cTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGTCCCCAGACAAAAATGGCGTCGGc                                                                                                                       <  2:33204/150‑1 (MQ=255)

                                   gggTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGc                                                                                                                   <  1:2514852/150‑1 (MQ=255)

                                      gcaacgAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCg                                                                                                                >  2:1265063/6‑150 (MQ=255)

                                        cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                              <  2:1041017/150‑1 (MQ=255)

                                        cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGAATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                              <  1:3966271/150‑1 (MQ=255)

                                         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                             >  2:3687036/1‑150 (MQ=255)

                                         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                             <  1:2454152/150‑1 (MQ=255)

                                         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                             >  2:4265612/1‑150 (MQ=255)

                                         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                             <  1:2581445/150‑1 (MQ=255)

                                         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGAGCTCGAAg                                                                                                             >  2:2170066/1‑150 (MQ=255)

                                          ggAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgt                                                                                                            >  1:1714605/1‑150 (MQ=255)

                                           gAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtc                                                                                                           >  2:2659731/1‑150 (MQ=255)

                                           gAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtc                                                                                                           >  1:2653133/1‑150 (MQ=255)

                                            acccgcTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtcg                                                                                                          >  2:3163121/4‑150 (MQ=255)

                                            aaGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtcg                                                                                                          <  1:3462170/150‑1 (MQ=255)

                                                  tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                    >  2:475047/1‑150 (MQ=255)

                                                  tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                    >  1:2716548/1‑150 (MQ=255)

                                                  tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCCGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                    >  1:3147046/1‑150 (MQ=255)

                                                      gACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑a                                                                                               >  2:2031952/1‑150 (MQ=255)

                                                       aCCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑Ac                                                                                              >  1:860692/1‑150 (MQ=255)

                                                          aCGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGt                                                                                           <  1:651250/150‑1 (MQ=255)

                                                           cGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTa                                                                                          >  1:1338981/1‑150 (MQ=255)

                                                                   gAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGcc                                                                                  <  2:4894692/150‑1 (MQ=255)

                                                                      cGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                               <  1:2887925/150‑1 (MQ=255)

                                                                              tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                       >  2:537889/1‑150 (MQ=255)

                                                                                cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                     <  1:2618999/150‑1 (MQ=255)

                                                                                 acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                    >  1:1042701/1‑150 (MQ=255)

                                                                                   accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                  <  2:2306839/150‑1 (MQ=255)

                                                                                    ccaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                 <  1:591406/150‑1 (MQ=255)

                                                                                         tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                            >  2:694469/1‑150 (MQ=255)

                                                                                              gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                       >  2:1879908/1‑150 (MQ=255)

                                                                                              gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                       >  2:4343128/1‑150 (MQ=255)

                                                                                               ccACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacg                                                      >  2:830706/1‑150 (MQ=255)

                                                                                                cACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgc                                                     >  2:5347513/1‑150 (MQ=255)

                                                                                                 aCGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgcc                                                    >  1:3475561/1‑150 (MQ=255)

                                                                                                    cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                 <  2:3756026/150‑1 (MQ=255)

                                                                                                     tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                <  1:5181672/150‑1 (MQ=255)

                                                                                                     tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                <  1:5111275/150‑1 (MQ=255)

                                                                                                     tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                >  1:420534/1‑150 (MQ=255)

                                                                                                       tACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTAGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGc                                              <  2:3829389/150‑1 (MQ=255)

                                                                                                        aCCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGcc                                             >  1:1766072/1‑150 (MQ=255)

                                                                                                                        ggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCg                             <  2:3009440/150‑1 (MQ=255)

                                                                                                                         gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                            <  2:2790975/150‑1 (MQ=255)

                                                                                                                         gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                            <  1:1916050/150‑1 (MQ=255)

                                                                                                                         gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                            <  1:1185856/150‑1 (MQ=255)

                                                                                                                          cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                           <  2:3857120/145‑2 (MQ=255)

                                                                                                                          cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                           <  2:4885896/145‑2 (MQ=255)

                                                                                                                             aGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                 >  1:3589351/1‑109 (MQ=255)

                                                                                                                              gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                       <  1:5361846/150‑1 (MQ=255)

                                                                                                                              gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                       >  1:361604/1‑150 (MQ=255)

                                                                                                                                aTGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCa                     <  2:1680453/150‑1 (MQ=255)

                                                                                                                                   cgcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAggg                  <  2:2399080/150‑1 (MQ=255)

                                                                                                                                    gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                               <  1:2809268/88‑1 (MQ=255)

                                                                                                                                    gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                               >  2:2809268/1‑88 (MQ=255)

                                                                                                                                     cgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGaa                <  2:1102996/150‑1 (MQ=255)

                                                                                                                                      ggatGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGaaa               <  1:2939829/147‑2 (MQ=255)

                                                                                                                                       ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                       <  1:2296565/125‑1 (MQ=255)

                                                                                                                                       ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                       >  2:2296565/1‑125 (MQ=255)

                                                                                                                                       ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgt              >  2:4015479/1‑150 (MQ=255)

                                                                                                                                          gATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgttgt           <  2:1913396/150‑1 (MQ=255)

                                                                                                                                            ttGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGttgttg         <  1:4602288/150‑1 (MQ=255)

                                                                                                                                            ttGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttc         <  1:2149738/150‑2 (MQ=255)

                                                                                                                                             tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg        >  2:1531606/1‑148 (MQ=255)

                                                                                                                                             tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg        >  2:1251424/1‑148 (MQ=255)

                                                                                                                                             tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg        <  1:1847330/150‑3 (MQ=255)

                                                                                                                                              ggTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTACTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGt       <  1:541350/150‑1 (MQ=255)

                                                                                                                                              ggTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGt       <  1:3384367/150‑1 (MQ=255)

                                                                                                                                               gTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTg      >  1:3339559/1‑150 (MQ=255)

                                                                                                                                                 gCCGGGGTTCCGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGcc    <  2:3147046/150‑1 (MQ=255)

                                                                                                                                                  ccGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCg   >  1:3681481/1‑150 (MQ=255)

GGTCACTGTTGGTCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGT  >  NC_002947/311534‑311830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`