BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	311,695	C→A	8.9%	W228L (TGG→TTG) ‡	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	311,695	0	C	A	8.9%	647.6 / 34.5	200	W228L (TGG→TTG) ‡	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (91/91); new base A (4/14); total (95/105)
Fisher's exact test for biased strand distribution p-value = 2.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

TCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAAC  >  NC_002947/311546‑311844

tCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGcccc                                                                                                                                                        >  1:2308561/1‑150 (MQ=255)

 cAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCa                                                                                                                                                       <  2:1726701/150‑1 (MQ=255)

 cAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCa                                                                                                                                                       >  2:2220821/1‑150 (MQ=255)

 cAGCATTTCCAGTGGGTTCGGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCa                                                                                                                                                       <  2:4176533/150‑1 (MQ=255)

  aGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAg                                                                                                                                                      >  1:1680453/1‑150 (MQ=255)

  aGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAg                                                                                                                                                      >  2:3271511/1‑150 (MQ=255)

   gCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGa                                                                                                                                                     <  2:2635054/150‑1 (MQ=255)

   gCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGa                                                                                                                                                     >  2:3511294/1‑150 (MQ=255)

     aTTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACa                                                                                                                                                   >  1:3456951/1‑150 (MQ=255)

          cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                              >  1:4259695/1‑150 (MQ=255)

          cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                              >  2:1826932/1‑150 (MQ=255)

          cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                              >  2:3451392/1‑150 (MQ=255)

            gTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTATACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATgg                                                                                                                                            >  2:5406883/1‑150 (MQ=255)

                    tGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGTGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCg                                                                                                                                    <  1:577326/150‑1 (MQ=255)

                       gggTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGc                                                                                                                                 <  1:2514852/150‑1 (MQ=255)

                            cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                            <  2:1041017/150‑1 (MQ=255)

                            cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGAATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                            <  1:3966271/150‑1 (MQ=255)

                             tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                           <  1:2581445/150‑1 (MQ=255)

                             tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                           >  2:3687036/1‑150 (MQ=255)

                             tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                           <  1:2454152/150‑1 (MQ=255)

                             tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                           >  1:3530669/1‑150 (MQ=255)

                             tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGAGCTCGAAg                                                                                                                           >  2:2170066/1‑150 (MQ=255)

                              ggAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgt                                                                                                                          >  1:1714605/1‑150 (MQ=255)

                               gAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtc                                                                                                                         >  2:2659731/1‑150 (MQ=255)

                                aaGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtcg                                                                                                                        <  1:3462170/150‑1 (MQ=255)

                                   cgcTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGtcgtcg                                                                                                                     >  2:4886850/1‑150 (MQ=255)

                                      tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                  <  1:2648068/150‑1 (MQ=255)

                                      tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                  <  2:3162716/150‑1 (MQ=255)

                                      tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                  >  2:475047/1‑150 (MQ=255)

                                      tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCCGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                  >  1:3147046/1‑150 (MQ=255)

                                           aCCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑Ac                                                                                                            >  1:3603045/1‑150 (MQ=255)

                                           aCCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑Ac                                                                                                            >  1:860692/1‑150 (MQ=255)

                                            ccACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACt                                                                                                           <  2:5131586/150‑1 (MQ=255)

                                            ccACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACt                                                                                                           <  1:3595084/150‑1 (MQ=255)

                                               cGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTa                                                                                                        >  1:1338981/1‑150 (MQ=255)

                                                     ttGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTg                                                                                                  <  2:1253486/150‑1 (MQ=255)

                                                       gAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGcc                                                                                                >  2:2618999/1‑150 (MQ=255)

                                                         gCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGcc                                                                                                <  2:1375721/148‑1 (MQ=255)

                                                          cGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                             <  1:5400436/150‑1 (MQ=255)

                                                          cGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                             <  1:2887925/150‑1 (MQ=255)

                                                                 aTGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACg                                                                                      >  2:1142252/1‑150 (MQ=255)

                                                                  tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                                     >  2:3178297/1‑150 (MQ=255)

                                                                  tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                                     >  2:537552/1‑150 (MQ=255)

                                                                    cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                   >  2:5285238/1‑150 (MQ=255)

                                                                     acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                  >  1:1042701/1‑150 (MQ=255)

                                                                       accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                <  2:2306839/150‑1 (MQ=255)

                                                                       accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                <  1:5233092/150‑1 (MQ=255)

                                                                           ccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCa                                                                            >  1:5357177/1‑150 (MQ=255)

                                                                            cTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCaa                                                                           >  2:2033693/1‑150 (MQ=255)

                                                                             tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                          <  2:3829754/150‑1 (MQ=255)

                                                                             tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                          >  2:694469/1‑150 (MQ=255)

                                                                                  gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCCacgccac                                                                     >  2:4342832/1‑150 (MQ=255)

                                                                                  gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                                     >  2:1879979/1‑150 (MQ=255)

                                                                                   ccACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacg                                                                    >  2:830706/1‑150 (MQ=255)

                                                                                    cACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgc                                                                   >  2:5347513/1‑150 (MQ=255)

                                                                                     aCGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgcc                                                                  >  1:3475561/1‑150 (MQ=255)

                                                                                        cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                               <  2:3756026/150‑1 (MQ=255)

                                                                                        cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                               <  2:3342901/150‑1 (MQ=255)

                                                                                         tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                              <  1:5111275/150‑1 (MQ=255)

                                                                                         tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                              >  1:4862958/1‑150 (MQ=255)

                                                                                         tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                              <  2:1284917/150‑1 (MQ=255)

                                                                                            aCCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGcc                                                           >  1:1766072/1‑150 (MQ=255)

                                                                                               gCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCa                                                        >  2:2858569/1‑150 (MQ=255)

                                                                                                cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                       <  2:496085/150‑1 (MQ=255)

                                                                                                  tCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                     >  1:4394451/1‑150 (MQ=255)

                                                                                                   cACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGc                                                    <  1:673100/150‑1 (MQ=255)

                                                                                                         gTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGt                                              >  1:412278/1‑150 (MQ=255)

                                                                                                           gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                            <  2:3603045/150‑1 (MQ=255)

                                                                                                           gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                            <  2:4862958/150‑1 (MQ=255)

                                                                                                             gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                          <  1:1185856/150‑1 (MQ=255)

                                                                                                             gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                          <  1:1916050/150‑1 (MQ=255)

                                                                                                             gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                          <  2:2790975/150‑1 (MQ=255)

                                                                                                             gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                          <  1:611892/150‑1 (MQ=255)

                                                                                                                gAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCa                                       >  1:4515812/1‑150 (MQ=255)

                                                                                                                 aacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAg                                      >  2:3384367/3‑150 (MQ=255)

                                                                                                                  gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                     <  2:3126731/150‑1 (MQ=255)

                                                                                                                  gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                     <  2:3819626/150‑1 (MQ=255)

                                                                                                                       cgcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAggg                                <  2:2399080/150‑1 (MQ=255)

                                                                                                                        gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                             <  1:2809268/88‑1 (MQ=255)

                                                                                                                        gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGa                               >  1:5320372/1‑150 (MQ=255)

                                                                                                                         cgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGaa                              <  2:1102996/150‑1 (MQ=255)

                                                                                                                          ggatGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGaaa                             <  1:2939829/147‑2 (MQ=255)

                                                                                                                           ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                     >  2:2296565/1‑125 (MQ=255)

                                                                                                                            atGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAatt                           <  1:2798487/149‑1 (MQ=255)

                                                                                                                              gATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgttgt                         <  2:1913405/150‑1 (MQ=255)

                                                                                                                                 tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                      <  1:1847330/150‑3 (MQ=255)

                                                                                                                                 tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                      >  2:1531606/1‑148 (MQ=255)

                                                                                                                                 tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                      <  1:4465773/150‑3 (MQ=255)

                                                                                                                                   gTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTg                    >  1:3339559/1‑150 (MQ=255)

                                                                                                                                    tGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGc                   <  2:2686471/150‑1 (MQ=255)

                                                                                                                                      ccGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCg                 >  1:3681481/1‑150 (MQ=255)

                                                                                                                                       cGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGt                <  1:2676809/150‑1 (MQ=255)

                                                                                                                                         gggTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg              >  1:1333434/1‑150 (MQ=255)

                                                                                                                                         gggTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg              >  1:1096766/1‑150 (MQ=255)

                                                                                                                                          ggTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAgg             >  2:3872207/1‑150 (MQ=255)

                                                                                                                                             tCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGgcgc          <  1:2220821/150‑1 (MQ=255)

                                                                                                                                                  ccccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCAcgccgc                                                                >  1:3796016/1‑91 (MQ=255)

                                                                                                                                                    ccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa   >  2:4420022/1‑150 (MQ=255)

                                                                                                                                                    ccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa   >  1:4222233/1‑150 (MQ=255)

                                                                                                                                                     cAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAAc  <  2:1494475/150‑1 (MQ=255)

TCAGCATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAAC  >  NC_002947/311546‑311844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`