BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	311,700	A→G	10.8%	F226F (TTT→TTC)	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	311,700	0	A	G	10.8%	544.2 / 41.5	195	F226F (TTT→TTC)	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (84/88); new base G (6/15); total (92/103)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 201 total aligned reads displayed.

ATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTT  >  NC_002947/311551‑311848

aTTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACa                                                                                                                                                       >  1:3457583/1‑150 (MQ=255)

aTTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACa                                                                                                                                                       >  2:150501/1‑150 (MQ=255)

  ttCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACaaa                                                                                                                                                     >  1:524788/1‑150 (MQ=255)

     cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                                  >  1:5335527/1‑150 (MQ=255)

     cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                                  >  2:3451392/1‑150 (MQ=255)

     cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                                  >  1:4259695/1‑150 (MQ=255)

     cAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAAt                                                                                                                                                  >  2:1826932/1‑150 (MQ=255)

      aGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATg                                                                                                                                                 >  1:5064580/1‑150 (MQ=255)

       gTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTATACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATgg                                                                                                                                                >  2:5406883/1‑150 (MQ=255)

        tGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGc                                                                                                                                               <  1:1742047/150‑1 (MQ=255)

        tGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGc                                                                                                                                               >  2:3731415/1‑150 (MQ=255)

          ggTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGt                                                                                                                                             <  2:1431704/150‑1 (MQ=255)

               tGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGTGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCg                                                                                                                                        <  1:577326/150‑1 (MQ=255)

                       cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                                <  2:1041017/150‑1 (MQ=255)

                       cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGAATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                                <  1:3966271/150‑1 (MQ=255)

                        tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                               <  1:2581445/150‑1 (MQ=255)

                        tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                               <  1:2454152/150‑1 (MQ=255)

                        tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                               >  1:3530669/1‑150 (MQ=255)

                         ggAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgt                                                                                                                              >  1:1714586/1‑150 (MQ=255)

                          gAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtc                                                                                                                             >  1:2653133/1‑150 (MQ=255)

                          gAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtc                                                                                                                             >  2:2659731/1‑150 (MQ=255)

                            aGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtcgt                                                                                                                           <  2:2757710/150‑1 (MQ=255)

                              cgcTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGtcgtcg                                                                                                                         >  2:4886850/1‑150 (MQ=255)

                                 tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                      >  1:2716548/1‑150 (MQ=255)

                                 tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                      >  2:475047/1‑150 (MQ=255)

                                 tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                      <  2:3162716/150‑1 (MQ=255)

                                 tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCCGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                      >  1:3147046/1‑150 (MQ=255)

                                     gACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑a                                                                                                                 >  2:2031952/1‑150 (MQ=255)

                                      aCCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑Ac                                                                                                                >  1:860692/1‑150 (MQ=255)

                                       ccACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACt                                                                                                               <  1:3595084/150‑1 (MQ=255)

                                       ccACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACt                                                                                                               <  2:5131586/150‑1 (MQ=255)

                                          cGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTa                                                                                                            >  1:1338981/1‑150 (MQ=255)

                                                ttGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTg                                                                                                      <  2:1253486/150‑1 (MQ=255)

                                                 tGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGc                                                                                                     <  1:2934812/150‑1 (MQ=255)

                                                     cGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                                 <  1:5400436/150‑1 (MQ=255)

                                                       ggTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAccc                                                                                               >  2:2399458/1‑150 (MQ=255)

                                                            aTGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACg                                                                                          >  2:1142252/1‑150 (MQ=255)

                                                             tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                                         >  2:537552/1‑150 (MQ=255)

                                                             tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                                         >  2:3178297/1‑150 (MQ=255)

                                                               cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                       <  1:2618999/150‑1 (MQ=255)

                                                               cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                       >  2:5285238/1‑150 (MQ=255)

                                                                acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                      >  1:1042701/1‑150 (MQ=255)

                                                                 caccacCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTg                                                                                     >  1:3241060/1‑150 (MQ=255)

                                                                  accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                    <  2:2306839/150‑1 (MQ=255)

                                                                        tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                              >  2:694469/1‑150 (MQ=255)

                                                                            tgcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgcca                                                                          <  2:2201134/149‑1 (MQ=255)

                                                                             gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCCacgccac                                                                         >  2:4342832/1‑150 (MQ=255)

                                                                             gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                                         >  2:1879908/1‑150 (MQ=255)

                                                                              ccACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacg                                                                        >  2:830706/1‑150 (MQ=255)

                                                                               cACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgc                                                                       >  2:5347513/1‑150 (MQ=255)

                                                                                aCGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgcc                                                                      >  1:3475558/1‑150 (MQ=255)

                                                                                   cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                                   <  2:3342901/150‑1 (MQ=255)

                                                                                    tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                  <  1:5181672/150‑1 (MQ=255)

                                                                                    tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                  <  2:1284917/150‑1 (MQ=255)

                                                                                    tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                  >  1:4862958/1‑150 (MQ=255)

                                                                                      tACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTAGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGc                                                                <  2:3829389/150‑1 (MQ=255)

                                                                                           cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                           <  2:496085/150‑1 (MQ=255)

                                                                                           cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                           >  1:284610/1‑150 (MQ=255)

                                                                                             tCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                         >  1:4394451/1‑150 (MQ=255)

                                                                                              cACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGc                                                        <  1:673100/150‑1 (MQ=255)

                                                                                              cACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGc                                                        >  2:3953223/1‑150 (MQ=255)

                                                                                                    gTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGt                                                  >  1:412278/1‑150 (MQ=255)

                                                                                                      gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                <  2:4862958/150‑1 (MQ=255)

                                                                                                       ggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCg                                               <  2:3009440/150‑1 (MQ=255)

                                                                                                        gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                              <  1:611892/150‑1 (MQ=255)

                                                                                                        gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                              <  1:1185856/150‑1 (MQ=255)

                                                                                                         cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                             <  2:3857120/145‑2 (MQ=255)

                                                                                                         cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                             <  2:2088191/145‑2 (MQ=255)

                                                                                                           gAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCa                                           >  1:4515812/1‑150 (MQ=255)

                                                                                                            aacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAg                                          >  2:3384367/3‑150 (MQ=255)

                                                                                                            aGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                                   <  2:3589351/109‑1 (MQ=255)

                                                                                                             gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                         <  1:5361846/150‑1 (MQ=255)

                                                                                                             gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                         <  2:3126731/150‑1 (MQ=255)

                                                                                                             gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                         >  1:361604/1‑150 (MQ=255)

                                                                                                              cATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTc                                        <  2:361604/150‑1 (MQ=255)

                                                                                                               aTGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCa                                       <  2:1680453/150‑1 (MQ=255)

                                                                                                                tGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAg                                      <  1:2803231/150‑1 (MQ=255)

                                                                                                                   gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                                 >  2:2809268/1‑88 (MQ=255)

                                                                                                                    cgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGaa                                  <  2:1102996/150‑1 (MQ=255)

                                                                                                                      ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                         <  1:2296565/125‑1 (MQ=255)

                                                                                                                      ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                         >  2:2296565/1‑125 (MQ=255)

                                                                                                                         gATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgttgt                             <  2:1913396/150‑1 (MQ=255)

                                                                                                                           ttGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttc                           <  1:2149738/150‑2 (MQ=255)

                                                                                                                            tGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                >  1:187281/1‑128 (MQ=255)

                                                                                                                            tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                          <  1:4465773/150‑3 (MQ=255)

                                                                                                                            tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                          <  1:1847329/150‑3 (MQ=255)

                                                                                                                             ggTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGt                         <  1:3384367/150‑1 (MQ=255)

                                                                                                                                 ccGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCg                     >  1:3681481/1‑150 (MQ=255)

                                                                                                                                    gggTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg                  >  1:1096766/1‑150 (MQ=255)

                                                                                                                                    gggTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg                  >  1:1333434/1‑150 (MQ=255)

                                                                                                                                           ggCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCa           >  1:3503488/1‑150 (MQ=255)

                                                                                                                                             ccccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCAcgccgc                                                                    <  2:3796016/91‑1 (MQ=255)

                                                                                                                                             ccccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCAcgccgc                                                                    >  1:3796016/1‑91 (MQ=255)

                                                                                                                                               ccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa       >  2:4420022/1‑150 (MQ=255)

                                                                                                                                               ccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa       >  1:4222233/1‑150 (MQ=255)

                                                                                                                                                acgACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACTCCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTGCCATAAGCGCGCACGaaa      >  1:3044811/3‑149 (MQ=255)

                                                                                                                                                   aCAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGt   >  1:2511125/1‑150 (MQ=255)

                                                                                                                                                    cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt  >  2:1315288/1‑150 (MQ=255)

                                                                                                                                                    cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt  <  2:4259695/150‑1 (MQ=255)

                                                                                                                                                    cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt  <  2:2308561/150‑1 (MQ=255)

ATTTCCAGTGGGTTCTGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTT  >  NC_002947/311551‑311848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`