BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	311,715	G→C	10.8%	H221Q (CAC→CAG)	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	311,715	0	G	C	10.8%	613.0 / 51.2	195	H221Q (CAC→CAG)	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (82/92); new base C (6/15); total (88/107)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 196 total aligned reads displayed.

TGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCG  >  NC_002947/311566‑311863

tGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGTGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCg                                                                                                                                                       <  1:577326/150‑1 (MQ=255)

        cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                                               <  2:763801/150‑1 (MQ=255)

        cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                                               <  2:1063632/150‑1 (MQ=255)

        cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                                               >  1:3126731/1‑150 (MQ=255)

        cTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGAATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGaa                                                                                                                                               <  1:3966271/150‑1 (MQ=255)

         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                                              <  1:2581445/150‑1 (MQ=255)

         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                                              <  1:2454152/150‑1 (MQ=255)

         tGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAg                                                                                                                                              >  2:3687036/1‑150 (MQ=255)

          ggAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgt                                                                                                                                             >  1:1714605/1‑150 (MQ=255)

           gAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtc                                                                                                                                            >  1:2653133/1‑150 (MQ=255)

           gAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtc                                                                                                                                            >  2:2659731/1‑150 (MQ=255)

            acccgcTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtcg                                                                                                                                           >  2:3163121/4‑150 (MQ=255)

            aaGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtcg                                                                                                                                           <  1:3462170/150‑1 (MQ=255)

             aGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAgtcgt                                                                                                                                          <  2:2757710/150‑1 (MQ=255)

                  tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                                     >  1:2716548/1‑150 (MQ=255)

                  tCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAg                                                                                                                                     <  2:3162716/150‑1 (MQ=255)

                       aCCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑Ac                                                                                                                               >  1:3603045/1‑150 (MQ=255)

                       aCCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑Ac                                                                                                                               >  1:860692/1‑150 (MQ=255)

                         cACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTg                                                                                                                             >  1:1208242/1‑150 (MQ=255)

                          aCGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGt                                                                                                                            >  2:4568972/1‑150 (MQ=255)

                           cGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTa                                                                                                                           >  1:1338969/1‑150 (MQ=255)

                                 ttGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTg                                                                                                                     <  2:1253486/150‑1 (MQ=255)

                                   gAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGcc                                                                                                                   <  2:4894692/150‑1 (MQ=255)

                                     gCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGcc                                                                                                                   >  1:1375721/1‑148 (MQ=255)

                                      cGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                                                <  1:2887925/150‑1 (MQ=255)

                                      cGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                                                <  1:5400436/150‑1 (MQ=255)

                                        ggTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAccc                                                                                                              >  2:2399458/1‑150 (MQ=255)

                                             aTGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACg                                                                                                         >  2:1142252/1‑150 (MQ=255)

                                              tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                                                        >  2:537552/1‑150 (MQ=255)

                                                cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                                      <  1:2618999/150‑1 (MQ=255)

                                                cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                                      >  2:5285238/1‑150 (MQ=255)

                                                 gccccaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGACGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                     >  1:3666457/4‑150 (MQ=255)

                                                 acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                     >  2:3384574/1‑150 (MQ=255)

                                                 acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                     >  1:1092420/1‑150 (MQ=255)

                                                 acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                     >  1:1042701/1‑150 (MQ=255)

                                                  caccacCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTg                                                                                                    >  1:3241060/1‑150 (MQ=255)

                                                   accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                                   <  2:2306839/150‑1 (MQ=255)

                                                        cTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCaa                                                                                              <  1:903978/150‑1 (MQ=255)

                                                        cTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCaa                                                                                              >  2:2033693/1‑150 (MQ=255)

                                                         tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                                             <  2:63729/150‑1 (MQ=255)

                                                             tgcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgcca                                                                                         <  2:2201134/149‑1 (MQ=255)

                                                              gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCCacgccac                                                                                        >  2:4342832/1‑150 (MQ=255)

                                                              gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                                                        >  2:1879979/1‑150 (MQ=255)

                                                                cACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgc                                                                                      >  2:5347513/1‑150 (MQ=255)

                                                                 aCGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgcc                                                                                     >  1:3475561/1‑150 (MQ=255)

                                                                    cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                                                  <  2:3342901/150‑1 (MQ=255)

                                                                     tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                 <  1:5181672/150‑1 (MQ=255)

                                                                     tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                 >  1:4862958/1‑150 (MQ=255)

                                                                     tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                 <  2:1284917/150‑1 (MQ=255)

                                                                       tACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTAGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGc                                                                               <  2:3829389/150‑1 (MQ=255)

                                                                               cACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGc                                                                       <  1:673100/150‑1 (MQ=255)

                                                                               cACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGc                                                                       >  2:3953223/1‑150 (MQ=255)

                                                                                     gTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGt                                                                 >  1:412278/1‑150 (MQ=255)

                                                                                       gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                               <  2:860692/150‑1 (MQ=255)

                                                                                       gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                               <  2:3603045/150‑1 (MQ=255)

                                                                                        ggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCg                                                              <  2:3009440/150‑1 (MQ=255)

                                                                                         gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                             <  1:611892/150‑1 (MQ=255)

                                                                                         gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                             <  1:1185687/150‑1 (MQ=255)

                                                                                          cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                                            <  2:4885896/145‑2 (MQ=255)

                                                                                          cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                                            <  2:3857120/145‑2 (MQ=255)

                                                                                             aGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                                                  >  1:3589351/1‑109 (MQ=255)

                                                                                              gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                                        <  2:3126731/150‑1 (MQ=255)

                                                                                              gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                                        <  1:5361846/150‑1 (MQ=255)

                                                                                              acATGCGGGGATGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGGTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGt                                                        <  2:4781816/149‑1 (MQ=255)

                                                                                                aTGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCa                                                      <  2:1680453/150‑1 (MQ=255)

                                                                                                    gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                                                >  2:2809268/1‑88 (MQ=255)

                                                                                                    gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                                                <  1:2809268/88‑1 (MQ=255)

                                                                                                    gcgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGa                                                  >  1:5320372/1‑150 (MQ=255)

                                                                                                     cgGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGaa                                                 <  2:1102996/150‑1 (MQ=255)

                                                                                                      ggatGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGaaa                                                <  1:2939829/147‑2 (MQ=255)

                                                                                                       ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                        >  2:2296565/1‑125 (MQ=255)

                                                                                                       ggTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                        <  1:2296565/125‑1 (MQ=255)

                                                                                                        atGATTGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAatt                                              <  1:2798487/149‑1 (MQ=255)

                                                                                                          gATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgttgt                                            <  2:1913405/150‑1 (MQ=255)

                                                                                                            ttGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttc                                          <  1:2149738/150‑2 (MQ=255)

                                                                                                             tGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                               >  1:187281/1‑128 (MQ=255)

                                                                                                             tGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                               <  2:187281/128‑1 (MQ=255)

                                                                                                             tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                                         <  1:1847329/150‑3 (MQ=255)

                                                                                                             tGGTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                                         <  1:4465773/150‑3 (MQ=255)

                                                                                                              ggTGCCAGGATTTTGCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGt                                        <  1:3384367/150‑1 (MQ=255)

                                                                                                                 gCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGcc                                     <  2:5064580/150‑1 (MQ=255)

                                                                                                                              ccccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCAcgccgc                                                                                   <  2:3796016/91‑1 (MQ=255)

                                                                                                                              ccccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCAcgccgc                                                                                   >  1:3796016/1‑91 (MQ=255)

                                                                                                                               cccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCCAAGGTG‑ACTGTACATTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGACTCATGGCACATGATCGAGCAGTCAGGGAAGATGATGGAGCCGTAGGCGCGCACGa                       >  1:436899/1‑150 (MQ=255)

                                                                                                                               ccacgACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTGCCATAAGCGCGCACGa                       >  1:4986358/5‑150 (MQ=255)

                                                                                                                                ccAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa                      >  1:4222233/1‑150 (MQ=255)

                                                                                                                                 cAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAAc                     <  2:1494475/150‑1 (MQ=255)

                                                                                                                                    aCAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGt                  >  1:2511125/1‑150 (MQ=255)

                                                                                                                                     cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                 >  2:1315288/1‑150 (MQ=255)

                                                                                                                                     cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                 <  1:3511294/150‑1 (MQ=255)

                                                                                                                                     cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                 <  1:3196537/150‑1 (MQ=255)

                                                                                                                                     cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                 <  2:2308561/150‑1 (MQ=255)

                                                                                                                                     cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                 <  2:4259695/150‑1 (MQ=255)

                                                                                                                                       aaaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTgg               <  2:4394451/150‑1 (MQ=255)

                                                                                                                                        aaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGt              <  2:523996/150‑1 (MQ=255)

                                                                                                                                          aTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTAc            <  1:3163121/150‑1 (MQ=255)

                                                                                                                                           tGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACa           <  2:2120215/150‑1 (MQ=255)

                                                                                                                                              cGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGt        <  1:4568972/150‑1 (MQ=255)

                                                                                                                                               gTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTa       >  1:4706220/1‑150 (MQ=255)

                                                                                                                                                   gCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTAcgcc   <  1:3731415/150‑1 (MQ=255)

TGGGGGTGCTGGAAGCGCTCCAGACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCAGACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCG  >  NC_002947/311566‑311863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`