BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	311,734	G→C	11.2%	T215S (ACC→AGC) ‡	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	311,734	0	G	C	11.2%	579.8 / 51.5	188	T215S (ACC→AGC) ‡	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (74/93); new base C (6/15); total (80/108)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 189 total aligned reads displayed.

GACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCC  >  NC_002947/311588‑311882

gACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑a                                                                                                                                                   >  2:2031952/1‑150 (MQ=255)

 aCCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑Ac                                                                                                                                                  >  1:3603045/1‑150 (MQ=255)

   cACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTg                                                                                                                                                >  1:1208242/1‑150 (MQ=255)

    aCGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGt                                                                                                                                               <  1:651250/150‑1 (MQ=255)

     cGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTa                                                                                                                                              >  1:1338981/1‑150 (MQ=255)

            tGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGc                                                                                                                                       <  1:2934812/150‑1 (MQ=255)

             gAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGcc                                                                                                                                      <  2:4894692/150‑1 (MQ=255)

                cGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAc                                                                                                                                   <  1:5400436/150‑1 (MQ=255)

                  ggTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGAccc                                                                                                                                 >  2:2399458/1‑150 (MQ=255)

                       aTGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACg                                                                                                                            >  2:1142252/1‑150 (MQ=255)

                        tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                                                                           >  2:537889/1‑150 (MQ=255)

                          cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                                                         >  2:5285238/1‑150 (MQ=255)

                          cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                                                         <  1:2618999/150‑1 (MQ=255)

                           acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                                        >  1:1042701/1‑150 (MQ=255)

                           acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                                        >  1:1092420/1‑150 (MQ=255)

                            caccacCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTg                                                                                                                       >  1:3241060/1‑150 (MQ=255)

                             accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                                                      <  2:2306839/150‑1 (MQ=255)

                             accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                                                      <  1:5233092/150‑1 (MQ=255)

                              ccaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                                                                     <  1:591406/150‑1 (MQ=255)

                                 ccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCa                                                                                                                  >  1:5357177/1‑150 (MQ=255)

                                  cTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCaa                                                                                                                 <  1:903978/150‑1 (MQ=255)

                                   tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                                                                >  2:694469/1‑150 (MQ=255)

                                   tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                                                                <  2:3829754/150‑1 (MQ=255)

                                       tgcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgcca                                                                                                            <  2:2201134/149‑1 (MQ=255)

                                        gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCCacgccac                                                                                                           >  2:4342832/1‑150 (MQ=255)

                                        gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                                                                           >  2:1879979/1‑150 (MQ=255)

                                          cACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgc                                                                                                         >  2:5347513/1‑150 (MQ=255)

                                           aCGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgcc                                                                                                        >  1:3475558/1‑150 (MQ=255)

                                              cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                                                                     <  2:3756026/150‑1 (MQ=255)

                                               tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                    <  1:5111275/150‑1 (MQ=255)

                                               tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                    <  2:1284917/150‑1 (MQ=255)

                                                 tACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGc                                                                                                  <  1:3753003/150‑1 (MQ=255)

                                                     gCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCa                                                                                              >  2:2858569/1‑150 (MQ=255)

                                                      cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                                                             >  1:284610/1‑150 (MQ=255)

                                                        tCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                           >  1:4394451/1‑150 (MQ=255)

                                                               gTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGt                                                                                    >  1:412278/1‑150 (MQ=255)

                                                                 gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                  <  2:4862958/150‑1 (MQ=255)

                                                                 gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                  <  2:860692/150‑1 (MQ=255)

                                                                   gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                                                <  1:1185687/150‑1 (MQ=255)

                                                                   gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                                                <  1:1916050/150‑1 (MQ=255)

                                                                   gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                                                <  2:2790975/150‑1 (MQ=255)

                                                                    cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                                                               <  2:2088191/145‑2 (MQ=255)

                                                                      gAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCa                                                                             >  1:4515812/1‑150 (MQ=255)

                                                                       aacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAg                                                                            >  2:3384367/3‑150 (MQ=255)

                                                                       aGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                                                                     <  2:3589351/109‑1 (MQ=255)

                                                                       aGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                                                                     >  1:3589351/1‑109 (MQ=255)

                                                                        gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                                                           <  2:3819626/150‑1 (MQ=255)

                                                                        gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                                                           <  1:5361846/150‑1 (MQ=255)

                                                                        gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                                                           >  1:361604/1‑150 (MQ=255)

                                                                        acATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGGTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGt                                                                           <  2:4781816/149‑1 (MQ=255)

                                                                         cATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTc                                                                          <  2:361604/150‑1 (MQ=255)

                                                                          aTGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCa                                                                         <  2:1680453/150‑1 (MQ=255)

                                                                           tGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAg                                                                        <  1:2803231/150‑1 (MQ=255)

                                                                                ggatGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGaaa                                                                   <  1:2939829/147‑2 (MQ=255)

                                                                                 ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                           <  1:2296565/125‑1 (MQ=255)

                                                                                 ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                           >  2:2296565/1‑125 (MQ=255)

                                                                                 ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgt                                                                  >  2:4015479/1‑150 (MQ=255)

                                                                                  atGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAatt                                                                 <  2:1112865/149‑1 (MQ=255)

                                                                                    gATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgttgt                                                               <  2:1913405/150‑1 (MQ=255)

                                                                                      ttGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGttgttg                                                             <  1:4602288/150‑1 (MQ=255)

                                                                                      ttGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGttgttg                                                             <  1:379824/150‑1 (MQ=255)

                                                                                      ttGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttc                                                             <  1:2149738/150‑2 (MQ=255)

                                                                                       tGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                  <  2:187281/128‑1 (MQ=255)

                                                                                       tGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                                                            <  1:1847330/150‑3 (MQ=255)

                                                                                       tGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                                                            >  2:1251424/1‑148 (MQ=255)

                                                                                         gTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTg                                                          >  1:3339559/1‑150 (MQ=255)

                                                                                          tGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGc                                                         <  2:2686471/150‑1 (MQ=255)

                                                                                           gCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGcc                                                        <  2:5064580/150‑1 (MQ=255)

                                                                                           gCCGGGGTTCCGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGcc                                                        <  2:3147046/150‑1 (MQ=255)

                                                                                            ccGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCg                                                       >  1:3681481/1‑150 (MQ=255)

                                                                                               gggTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg                                                    >  1:1333434/1‑150 (MQ=255)

                                                                                                ggTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAgg                                                   >  2:3872207/1‑150 (MQ=255)

                                                                                                    tttgCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTGCCATAAgcgc                                                <  1:402085/147‑1 (MQ=255)

                                                                                                     tGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGTCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGgcgcgc                                              >  2:3096652/1‑150 (MQ=255)

                                                                                                      ggCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCa                                             >  1:3503488/1‑150 (MQ=255)

                                                                                                       gCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCAc                                            <  1:694469/150‑1 (MQ=255)

                                                                                                          ccA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa                                         >  1:4222233/1‑150 (MQ=255)

                                                                                                          ccA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa                                         >  2:4420022/1‑150 (MQ=255)

                                                                                                           cA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAAc                                        <  2:1494475/150‑1 (MQ=255)

                                                                                                               aCAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGt                                     >  1:2511125/1‑150 (MQ=255)

                                                                                                                cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                                    <  1:3196537/150‑1 (MQ=255)

                                                                                                                cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                                    >  2:1315288/1‑150 (MQ=255)

                                                                                                                cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                                    <  2:2308561/150‑1 (MQ=255)

                                                                                                                  aaaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTgg                                  <  2:1092420/150‑1 (MQ=255)

                                                                                                                   aaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGt                                 <  2:523996/150‑1 (MQ=255)

                                                                                                                   aaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGt                                 <  1:4886850/150‑1 (MQ=255)

                                                                                                                     aTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTAc                               <  1:3163121/150‑1 (MQ=255)

                                                                                                                       ggCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAg                             <  1:2721429/150‑1 (MQ=255)

                                                                                                                               cGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTAcgccg                     >  1:484852/1‑150 (MQ=255)

                                                                                                                                 tGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCt                   <  1:2033693/150‑1 (MQ=255)

                                                                                                                                   cTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTc                 <  2:3456951/150‑1 (MQ=255)

                                                                                                                                    tCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCg                <  2:3241060/150‑1 (MQ=255)

                                                                                                                                    tCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCg                >  2:1109370/1‑150 (MQ=255)

                                                                                                                                        aGTCGTCGTAGGTG‑TCTTTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCGGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGc            <  1:1538650/150‑1 (MQ=255)

                                                                                                                                         gtcgtcGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCt           <  1:4420022/150‑1 (MQ=255)

                                                                                                                                         gtcgtcGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCt           >  2:2783412/1‑150 (MQ=255)

                                                                                                                                          tcgtcgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTcacccagc          <  1:4265612/150‑8 (MQ=255)

                                                                                                                                            gtcgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGc        >  1:4744636/1‑150 (MQ=255)

                                                                                                                                                 aGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTc   >  1:1823669/1‑150 (MQ=255)

                                                                                                                                                  ggTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTcc  >  2:4347772/1‑150 (MQ=255)

GACCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCC  >  NC_002947/311588‑311882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`