BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	311,739	T→A	10.8%	T213T (ACA→ACT)	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	311,739	0	T	A	10.8%	574.7 / 48.5	194	T213T (ACA→ACT)	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (72/101); new base A (6/15); total (78/116)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 197 total aligned reads displayed.

CCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGT  >  NC_002947/311590‑311888

ccACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACt                                                                                                                                                       <  2:5131586/150‑1 (MQ=255)

ccACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACt                                                                                                                                                       <  1:3595084/150‑1 (MQ=255)

 cACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTg                                                                                                                                                      >  1:1208242/1‑150 (MQ=255)

  aCGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGt                                                                                                                                                     <  1:651250/150‑1 (MQ=255)

  aCGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGt                                                                                                                                                     >  2:4568972/1‑150 (MQ=255)

   cGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTa                                                                                                                                                    >  1:1338981/1‑150 (MQ=255)

          tGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGc                                                                                                                                             <  1:2934812/150‑1 (MQ=255)

           gAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGcc                                                                                                                                            >  2:2618999/1‑150 (MQ=255)

                     aTGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACg                                                                                                                                  >  1:4252207/1‑150 (MQ=255)

                     aTGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACg                                                                                                                                  >  2:1142252/1‑150 (MQ=255)

                      tGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGc                                                                                                                                 >  2:537552/1‑150 (MQ=255)

                        cacaCCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCtt                                                                                                                               <  1:2618999/150‑1 (MQ=255)

                         gccccaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGACGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                                              >  1:3666457/4‑150 (MQ=255)

                         acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                                              >  1:1092420/1‑150 (MQ=255)

                         acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                                              >  2:3384574/1‑150 (MQ=255)

                         acacCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCttt                                                                                                                              >  1:1042701/1‑150 (MQ=255)

                           accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                                                            <  1:5233092/150‑1 (MQ=255)

                           accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                                                            <  2:2306846/150‑1 (MQ=255)

                           accaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGc                                                                                                                            <  2:2306805/150‑1 (MQ=255)

                            ccaccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGcc                                                                                                                           <  1:591406/150‑1 (MQ=255)

                               ccTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCa                                                                                                                        >  1:5357177/1‑150 (MQ=255)

                                 tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                                                                      <  2:3829754/150‑1 (MQ=255)

                                 tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                                                                      <  2:63729/150‑1 (MQ=255)

                                     tgcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgcca                                                                                                                  <  2:2201134/149‑1 (MQ=255)

                                      gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCCacgccac                                                                                                                 >  2:4342832/1‑150 (MQ=255)

                                      gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                                                                                 >  2:1879979/1‑150 (MQ=255)

                                         aCGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgcc                                                                                                              >  1:3475561/1‑150 (MQ=255)

                                            cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                                                                           <  2:3756026/150‑1 (MQ=255)

                                            cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                                                                           <  2:3342901/150‑1 (MQ=255)

                                             tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                          <  1:5111275/150‑1 (MQ=255)

                                             tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                          >  1:420534/1‑150 (MQ=255)

                                             tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                          <  2:1284917/150‑1 (MQ=255)

                                                aCCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGcc                                                                                                       >  1:1766072/1‑150 (MQ=255)

                                                   gCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCa                                                                                                    >  2:2858569/1‑150 (MQ=255)

                                                    cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                                                                   <  2:496085/150‑1 (MQ=255)

                                                    cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                                                                   >  1:284610/1‑150 (MQ=255)

                                                             gTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGt                                                                                          >  1:412278/1‑150 (MQ=255)

                                                               gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                        <  2:860692/150‑1 (MQ=255)

                                                               gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                        <  2:3603045/150‑1 (MQ=255)

                                                                 gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                                                      <  1:1185687/150‑1 (MQ=255)

                                                                 gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                                                      <  2:2790975/150‑1 (MQ=255)

                                                                  cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                                                                     <  2:4885896/145‑2 (MQ=255)

                                                                  cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                                                                     <  2:2088191/145‑2 (MQ=255)

                                                                    gAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCa                                                                                   >  1:4515812/1‑150 (MQ=255)

                                                                    gAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCa                                                                                   <  1:5366498/150‑1 (MQ=255)

                                                                      gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                                                                 <  2:3819626/150‑1 (MQ=255)

                                                                        aTGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCa                                                                               <  2:1680453/150‑1 (MQ=255)

                                                                         tGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAg                                                                              <  1:2803231/150‑1 (MQ=255)

                                                                           cgcgGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAggg                                                                            <  2:2399080/150‑1 (MQ=255)

                                                                             cgGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGaa                                                                          <  2:1102996/150‑1 (MQ=255)

                                                                               ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                                 >  2:2296565/1‑125 (MQ=255)

                                                                               ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                                 <  1:2296565/125‑1 (MQ=255)

                                                                               ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgt                                                                        >  2:4015479/1‑150 (MQ=255)

                                                                                  gATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgttgt                                                                     <  2:1913405/150‑1 (MQ=255)

                                                                                    ttGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGttgttg                                                                   <  1:379824/150‑1 (MQ=255)

                                                                                    ttGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGttgttg                                                                   <  1:4602288/150‑1 (MQ=255)

                                                                                     tGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                        >  1:187281/1‑128 (MQ=255)

                                                                                     tGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                        <  2:187281/128‑1 (MQ=255)

                                                                                     tGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                                                                  >  2:1251424/1‑148 (MQ=255)

                                                                                     tGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAattgttcg                                                                  <  1:1847329/150‑3 (MQ=255)

                                                                                      ggTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGt                                                                 <  1:3384367/150‑1 (MQ=255)

                                                                                         gCCGGGGTTCCGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGcc                                                              <  2:3147046/150‑1 (MQ=255)

                                                                                             gggTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg                                                          >  1:1333434/1‑150 (MQ=255)

                                                                                              ggTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAgg                                                         >  2:3872207/1‑150 (MQ=255)

                                                                                                  tttgCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTGCCATAAgcgc                                                      <  1:402085/147‑1 (MQ=255)

                                                                                                   tGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGTCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGgcgcgc                                                    >  2:3096652/1‑150 (MQ=255)

                                                                                                     gCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCAc                                                  <  2:5335527/150‑1 (MQ=255)

                                                                                                     gCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCAc                                                  <  1:694469/150‑1 (MQ=255)

                                                                                                       cccA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCCAAGGTG‑ACTGTACATTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGACTCATGGCACATGATCGAGCAGTCAGGGAAGATGATGGAGCCGTAGGCGCGCACGa                                                >  1:436899/1‑150 (MQ=255)

                                                                                                        ccA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGaa                                               >  2:4420022/1‑150 (MQ=255)

                                                                                                         cA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAAc                                              <  2:1494475/150‑1 (MQ=255)

                                                                                                          acgACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACTCCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTGCCATAAGCGCGCACGaaa                                              >  1:3044811/3‑149 (MQ=255)

                                                                                                             aCAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGt                                           >  1:2511125/1‑150 (MQ=255)

                                                                                                              cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                                          >  2:1315288/1‑150 (MQ=255)

                                                                                                              cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                                          <  1:3511294/150‑1 (MQ=255)

                                                                                                                 aaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGt                                       <  1:4886850/150‑1 (MQ=255)

                                                                                                                 aaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGt                                       <  2:524700/150‑1 (MQ=255)

                                                                                                                  aaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTa                                      <  1:3178297/150‑1 (MQ=255)

                                                                                                                     ggCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAg                                   <  1:2721429/150‑1 (MQ=255)

                                                                                                                            gCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTAcgcc                            <  1:3731415/150‑1 (MQ=255)

                                                                                                                             cGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTAcgccg                           >  1:484852/1‑150 (MQ=255)

                                                                                                                             cGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTAcgccg                           >  1:3212009/1‑150 (MQ=255)

                                                                                                                                tcTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCtt                        <  2:2716548/149‑1 (MQ=255)

                                                                                                                                 cTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTc                       <  2:3457583/150‑1 (MQ=255)

                                                                                                                                  tCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCg                      >  2:1109451/1‑150 (MQ=255)

                                                                                                                                    gAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGtt                    >  1:2081504/1‑150 (MQ=255)

                                                                                                                                       gtcgtcGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCt                 <  1:4420022/150‑1 (MQ=255)

                                                                                                                                        tcgtcgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTg                <  1:3271511/150‑1 (MQ=255)

                                                                                                                                        tcgtcgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTg                >  2:1045586/1‑150 (MQ=255)

                                                                                                                                          gtcgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGc              >  1:4744636/1‑150 (MQ=255)

                                                                                                                                                ggTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTcc        >  2:4347772/1‑150 (MQ=255)

                                                                                                                                                 atG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTccc       <  1:3788868/149‑1 (MQ=255)

                                                                                                                                                   g‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGa     <  2:1714605/150‑1 (MQ=255)

                                                                                                                                                   g‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGa     <  1:150501/150‑1 (MQ=255)

                                                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAg    <  1:2031952/150‑1 (MQ=255)

                                                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAg    <  2:484852/150‑1 (MQ=255)

                                                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAg    <  1:2170066/150‑1 (MQ=255)

                                                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAg    <  2:3212009/150‑1 (MQ=255)

                                                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTAATGGCTCATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCgtg    <  1:5406883/150‑3 (MQ=255)

                                                                                                                                                       tGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGt  >  2:723154/1‑150 (MQ=255)

CCACGCTCCTTGAGCGGGTTGATGCACACCACCTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGT  >  NC_002947/311590‑311888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`