BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,107,638	G→A	5.1%	P815L (CCG→CTG)	ydiJ ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,107,638	0	G	A	5.1%	684.2 / 15.3	214	P815L (CCG→CTG)	ydiJ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (88/115); new base A (5/6); total (93/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GATACACCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCCGG  >  NC_002947/5107489‑5107783

gATACACCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCCTGCCGTTGGCGCTGTACg                                                                                                                                                   >  1:494126/1‑150 (MQ=255)

     acCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGcc                                                                                                                                              <  1:3502647/150‑1 (MQ=255)

      ccAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCa                                                                                                                                             <  1:308961/150‑1 (MQ=255)

       cAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAg                                                                                                                                            >  2:220108/1‑150 (MQ=255)

       cAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAg                                                                                                                                            <  1:2104501/150‑1 (MQ=255)

       cAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCCGTACAGCGCCaa                                                                                                                                            <  1:4421205/150‑2 (MQ=255)

       cAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCCGTACAGCGCCaa                                                                                                                                            <  2:5267686/150‑2 (MQ=255)

         tcGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGCTGGCGCCGTACAGCGCCaacg                                                                                                                                          <  2:3306265/149‑4 (MQ=255)

          cGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGaa                                                                                                                                         <  1:3895432/150‑1 (MQ=255)

          cGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGCTGGCGCCGTACAGCGCCaacgg                                                                                                                                         <  2:3943289/150‑5 (MQ=255)

              aTCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATAcc                                                                                                                                     >  2:4409493/1‑150 (MQ=255)

               tCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATAccc                                                                                                                                    >  2:4199173/1‑150 (MQ=255)

                    gggTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTgg                                                                                                                               <  1:3736429/150‑1 (MQ=255)

                     ggTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGc                                                                                                                              >  1:3592165/1‑150 (MQ=255)

                       tCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGccc                                                                                                                            >  1:3802523/1‑150 (MQ=255)

                              ccaccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGt                                                                                                                     <  2:3873557/150‑1 (MQ=255)

                              ccaccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGt                                                                                                                     <  1:272888/150‑1 (MQ=255)

                               caccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAg                                                                                                                                            >  2:1909026/1‑126 (MQ=255)

                               caccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAg                                                                                                                                            <  1:1909026/126‑1 (MQ=255)

                               caccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTg                                                                                                                    <  2:4497678/150‑1 (MQ=255)

                               caccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTg                                                                                                                    >  1:3459448/1‑150 (MQ=255)

                                accaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                   <  1:749671/150‑1 (MQ=255)

                                accaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                   <  1:3671277/150‑1 (MQ=255)

                                accaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                   <  1:3250593/150‑1 (MQ=255)

                                accaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCATGTACGTGCAACGGCTTGACGATGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                   >  1:3328285/1‑150 (MQ=255)

                                     cGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAg                                                                                                              <  2:5444229/150‑1 (MQ=255)

                                          cacCTCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCATTGGCAAACGCGCCAAGTAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGa                                                                                                         <  1:2880899/150‑1 (MQ=255)

                                          cacCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGTAAGGTCGa                                                                                                         <  1:4466269/150‑1 (MQ=255)

                                          cacCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGa                                                                                                         <  2:2263218/150‑1 (MQ=255)

                                             cGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCa                                                                                                                                             <  2:1628952/111‑1 (MQ=255)

                                             cGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCa                                                                                                                                             >  1:1628952/1‑111 (MQ=255)

                                             cGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGa                                                                                                      >  2:3479687/1‑150 (MQ=255)

                                              gCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGaa                                                                                                     <  2:2378339/150‑1 (MQ=255)

                                               cAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAg                                                                                                    <  2:3893187/150‑1 (MQ=255)

                                               cAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAg                                                                                                    <  1:2641521/150‑1 (MQ=255)

                                                 aTCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGc                                                                                                  <  1:1257373/150‑1 (MQ=255)

                                                           gCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACagcagc                                                                                        <  1:4166004/150‑1 (MQ=255)

                                                                  gCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGttt                                                                                 <  2:3880344/150‑1 (MQ=255)

                                                                    tGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCg                                                                               <  1:4013084/150‑1 (MQ=255)

                                                                    tGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCg                                                                               <  1:860115/150‑1 (MQ=255)

                                                                      cGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                             <  2:3063482/150‑1 (MQ=255)

                                                                      cGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                             <  1:1533355/150‑1 (MQ=255)

                                                                      cGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                             <  2:1199952/150‑1 (MQ=255)

                                                                        tGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGttcatcgac                                                                           <  1:1359849/150‑8 (MQ=255)

                                                                        tGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGttcatcgac                                                                           <  1:1360037/150‑8 (MQ=255)

                                                                        tGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCGGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCCGCGGCGGGTCGAAGt                                                                           >  2:4548070/1‑150 (MQ=255)

                                                                           cGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGc                                                                        <  1:2473292/150‑1 (MQ=255)

                                                                             ttGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                      <  1:2927874/150‑1 (MQ=255)

                                                                             ttGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                      >  2:1801602/1‑150 (MQ=255)

                                                                             ttGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                      <  1:551744/150‑1 (MQ=255)

                                                                                cGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTg                                                                   >  1:275532/1‑150 (MQ=255)

                                                                                 ggATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGa                                                                  >  2:3578470/1‑150 (MQ=255)

                                                                                   aTCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAg                                                                <  2:1302353/150‑1 (MQ=255)

                                                                                   aTCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAg                                                                <  2:816805/150‑1 (MQ=255)

                                                                                    tCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAg                                                                                                                                            <  2:2944476/73‑1 (MQ=255)

                                                                                       cTGCCTTGGCAAACGCGCCAAGGAACCCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGt                                                            >  2:1125709/1‑150 (MQ=255)

                                                                                       cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGTCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGGTTCGACGTAGCGGGTGAAGGCGt                                                            >  1:232287/1‑150 (MQ=255)

                                                                                       cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGTAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGt                                                            >  2:1711421/1‑150 (MQ=255)

                                                                                       cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                             >  1:5264943/1‑133 (MQ=255)

                                                                                       cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGt                                                            <  2:4896834/150‑1 (MQ=255)

                                                                                       cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGt                                                            >  2:268843/1‑150 (MQ=255)

                                                                                       cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGt                                                            >  1:1951247/1‑150 (MQ=255)

                                                                                           cTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATAcc                                                                                                                                     <  2:4838252/73‑1 (MQ=255)

                                                                                           cTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATAcc                                                                                                                                     >  1:4838252/1‑73 (MQ=255)

                                                                                            ttGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGa                                                                                                         >  1:4353589/1‑100 (MQ=255)

                                                                                            ttGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCt                                                         <  1:4306701/148‑1 (MQ=255)

                                                                                            ttGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGc                                                       <  1:1991822/150‑1 (MQ=255)

                                                                                              ggCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCAc                                                     >  2:1069942/1‑150 (MQ=255)

                                                                                                   aCGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCa                                                >  2:213359/1‑150 (MQ=255)

                                                                                                      cgcCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGa                                             <  1:3434116/150‑1 (MQ=255)

                                                                                                       gcCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                      <  2:2737599/124‑1 (MQ=255)

                                                                                                       gcCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCCGctactt                                            <  2:2494361/150‑6 (MQ=255)

                                                                                                        ccAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtg                                           <  2:1590146/150‑1 (MQ=255)

                                                                                                         cAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTg                                                                                                                    <  2:3701316/76‑1 (MQ=255)

                                                                                                          aaGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtgct                                         >  2:5192907/1‑150 (MQ=255)

                                                                                                           aGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCCAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtgctg                                        >  1:2343442/1‑150 (MQ=255)

                                                                                                            ggAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtgctgc                                       >  1:1045583/1‑150 (MQ=255)

                                                                                                              taaaccTGTACGTGCAACGTCTTGGCGTTGGCGCTGTACGGCGCCATGAATACCCGGTGGCGCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGTAGTAGCGGTTGAAGGCGTCCTGCACCAGCACGATGCTgcgc                                     <  1:391526/146‑1 (MQ=255)

                                                                                                              aaaCCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtg                                           <  2:3185051/144‑1 (MQ=255)

                                                                                                              aaaCCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtg                                           >  1:456988/1‑144 (MQ=255)

                                                                                                              aaaCCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtg                                           >  1:3185051/1‑144 (MQ=255)

                                                                                                              aaaCCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAtg                                           <  2:456988/144‑1 (MQ=255)

                                                                                                                 cccTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTcg                                  <  2:3510841/150‑1 (MQ=255)

                                                                                                                   cTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGc                                                                                                                        >  2:428675/1‑62 (MQ=255)

                                                                                                                       agcgccAACGGCAAGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTgg                            >  2:2088674/6‑150 (MQ=255)

                                                                                                                       aCGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTgg                            <  2:519564/150‑1 (MQ=255)

                                                                                                                          tGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGcc                         >  1:2050263/1‑150 (MQ=255)

                                                                                                                           gCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTggtgca                        <  1:3046914/150‑5 (MQ=255)

                                                                                                                           gCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCg                        <  1:3126189/150‑1 (MQ=255)

                                                                                                                           gCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCg                        >  2:4844092/1‑150 (MQ=255)

                                                                                                                             aaCGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCggg                      <  1:1739927/150‑1 (MQ=255)

                                                                                                                               cGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGt                    >  2:843230/1‑150 (MQ=255)

                                                                                                                                         ctGGCGCCGTACAGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCgcgc          >  1:5181287/2‑150 (MQ=255)

                                                                                                                                          tGGCGCCGTACAGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCa         >  1:803458/1‑150 (MQ=255)

                                                                                                                                              gcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGc     <  1:2208419/150‑1 (MQ=255)

                                                                                                                                              gcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGc     >  1:3508412/1‑150 (MQ=255)

                                                                                                                                              gcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGc     >  2:2330337/1‑150 (MQ=255)

                                                                                                                                               cTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGcc    <  1:220108/150‑1 (MQ=255)

                                                                                                                                                 gTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCCgg  <  1:4548040/150‑1 (MQ=255)

                                                                                                                                                 gTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCCgg  <  1:4548094/150‑1 (MQ=255)

GATACACCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCCGG  >  NC_002947/5107489‑5107783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`