BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,121,165	G→C	5.2%	L323L (CTG→CTC)	PP_4505 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,121,165	0	G	C	5.2%	717.7 / 20.5	213	L323L (CTG→CTC)	PP_4505	sensor histidine kinase
Reads supporting (aligned to +/- strand): ref base G (107/95); new base C (6/5); total (113/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 216 total aligned reads displayed.

GACCGCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGATCA  >  NC_002947/5121016‑5121313

gACCGCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTg                                                                                                                                                      >  1:388855/1‑150 (MQ=255)

    gCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAgg                                                                                                                                                  <  2:4813949/150‑1 (MQ=255)

    gCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAgg                                                                                                                                                  <  1:2209377/150‑1 (MQ=255)

    gCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAgg                                                                                                                                                  <  2:1475194/150‑1 (MQ=255)

       tGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGgcgc                                                                                                                                               >  1:3193730/1‑150 (MQ=255)

        ggAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCa                                                                                                                                              <  2:4680416/150‑1 (MQ=255)

        ggAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCa                                                                                                                                              >  2:2419141/1‑150 (MQ=255)

             aCCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAAGGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTCCAGGCGCacgtcc                                                                                                                                         <  1:3732472/150‑6 (MQ=255)

               cTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCACGGCCCTCCTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTCCAGGCGCacgtcctg                                                                                                                                       <  1:3262124/150‑8 (MQ=255)

               cTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGt                                                                                                                                       >  2:123133/1‑150 (MQ=255)

                 gATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGttt                                                                                                                                     >  1:340742/1‑150 (MQ=255)

                   tCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGa                                                                                                                                   <  1:412227/150‑1 (MQ=255)

                   tCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGa                                                                                                                                   <  1:680896/150‑1 (MQ=255)

                     aGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGc                                                                                                                                 <  1:2721768/150‑1 (MQ=255)

                       cGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCACCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCtt                                                                                                                               <  2:436523/150‑1 (MQ=255)

                        gAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTg                                                                                                                              >  2:1386351/1‑150 (MQ=255)

                         aaaTCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGc                                                                                                                             >  1:3559951/1‑150 (MQ=255)

                          aaTCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCa                                                                                                                            <  2:2173468/150‑1 (MQ=255)

                            tCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCCGGTTTGAGCTTGCAgg                                                                                                                          >  1:857163/1‑150 (MQ=255)

                              cTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGt                                                                                                                        >  1:540209/1‑150 (MQ=255)

                               tCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                       >  1:988520/1‑150 (MQ=255)

                               tCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                       >  1:249578/1‑150 (MQ=255)

                               tCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                       >  1:4715721/1‑150 (MQ=255)

                               tCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                       >  2:3075022/1‑150 (MQ=255)

                               tCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                       <  1:1555509/150‑1 (MQ=255)

                               tCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTCCAGGCGCacgtcc                                                                                                                                         <  2:1784958/132‑6 (MQ=255)

                               tCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTAAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGAAGGGtt                                                                                                                       >  1:4580167/1‑150 (MQ=255)

                                   ccTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGcc                                                                                                                   >  1:2821187/1‑150 (MQ=255)

                                                gATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAg                                                                                                      >  1:3065617/1‑150 (MQ=255)

                                                gATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAg                                                                                                      >  1:1439584/1‑150 (MQ=255)

                                                 aTGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGc                                                                                                     <  2:587106/150‑1 (MQ=255)

                                                      gAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGcc                                                                                                >  2:1669801/1‑150 (MQ=255)

                                                       aaCAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCg                                                                                               <  1:1711868/150‑1 (MQ=255)

                                                         cAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGtt                                                                                             <  1:5127744/150‑1 (MQ=255)

                                                          aaGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTg                                                                                            >  2:2017392/1‑150 (MQ=255)

                                                                  gCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACctg                                                                                    <  2:2877552/150‑1 (MQ=255)

                                                                    cGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACctgct                                                                                  >  2:1066444/1‑150 (MQ=255)

                                                                    cGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACctgct                                                                                  <  1:1386341/150‑1 (MQ=255)

                                                                      aaCCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCtgctgc                                                                                <  2:1966037/150‑1 (MQ=255)

                                                                       aCCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTgcg                                                                               >  1:4054830/1‑150 (MQ=255)

                                                                           gTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGa                                                                                                       >  1:1158406/1‑122 (MQ=255)

                                                                              gACCTCACTGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGcc                                                                        <  1:5218396/150‑1 (MQ=255)

                                                                              gACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGcc                                                                        <  2:340742/150‑1 (MQ=255)

                                                                                ccTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCt                                                                      <  2:2418714/150‑1 (MQ=255)

                                                                                 cTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGa                                                                                                                                   >  2:3175166/1‑88 (MQ=255)

                                                                                 cTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTg                                                                     >  1:5427759/1‑150 (MQ=255)

                                                                                 cTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTg                                                                     <  2:2821187/150‑1 (MQ=255)

                                                                                 cTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTg                                                                     >  2:3788047/1‑150 (MQ=255)

                                                                                  tCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTgc                                                                    >  1:2800981/1‑150 (MQ=255)

                                                                                  tCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTgc                                                                    >  2:4829108/1‑150 (MQ=255)

                                                                                  tCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTgc                                                                    <  1:4755217/150‑1 (MQ=255)

                                                                                   cAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTgcg                                                                   >  1:4296108/1‑150 (MQ=255)

                                                                                    aaTGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTgcgc                                                                  >  1:5055778/1‑150 (MQ=255)

                                                                                    aaTGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTgcgc                                                                  >  2:4274915/1‑150 (MQ=255)

                                                                                         ccTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACg                                                                                                               <  2:3929571/100‑1 (MQ=255)

                                                                                         ccTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACg                                                                                                               >  1:3929571/1‑100 (MQ=255)

                                                                                         ccTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCaa                                                             >  2:4967973/1‑150 (MQ=255)

                                                                                         ccTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCaa                                                             >  1:1015610/1‑150 (MQ=255)

                                                                                         ccTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCaa                                                             >  2:2230871/1‑150 (MQ=255)

                                                                                          cTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAAc                                                            >  2:460887/1‑150 (MQ=255)

                                                                                          cTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAAc                                                            <  1:4971758/150‑1 (MQ=255)

                                                                                           tACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAAcc                                                           >  1:2381986/1‑150 (MQ=255)

                                                                                            aCTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAAccc                                                          <  2:802697/150‑1 (MQ=255)

                                                                                              tCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCcgg                                                        <  1:1254074/150‑1 (MQ=255)

                                                                                               cGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCcggc                                                       <  2:5122982/150‑1 (MQ=255)

                                                                                                ggCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGtt                                                                                             <  2:4857705/111‑1 (MQ=255)

                                                                                                 gCAGCGTGCGCAAGGACGCCCTGCTTAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCcggcgg                                                     <  2:5109956/150‑1 (MQ=255)

                                                                                                   aGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGc                                                   <  2:1571115/150‑1 (MQ=255)

                                                                                                            aaGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGTCCTGCGCTTCAACCCGGCGGGCCAGCCGGTc                                          >  2:2814389/1‑150 (MQ=255)

                                                                                                               gACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                       <  1:4524634/150‑1 (MQ=255)

                                                                                                                 cGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGt                                     <  2:247102/150‑1 (MQ=255)

                                                                                                                    ccTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAg                                  >  1:1944998/1‑150 (MQ=255)

                                                                                                                    ccTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAg                                  <  2:388855/150‑1 (MQ=255)

                                                                                                                        ctgAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGcgcc                              <  2:540209/150‑1 (MQ=255)

                                                                                                                         tgAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGcgccg                             >  1:2617535/1‑150 (MQ=255)

                                                                                                                           aGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCtgctgc                                                                                <  2:382622/97‑1 (MQ=255)

                                                                                                                           aGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCtgctgc                                                                                >  1:382622/1‑97 (MQ=255)

                                                                                                                            gcgcgcCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGTAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCcgcgc                          <  2:1531018/150‑1 (MQ=255)

                                                                                                                            gcgcgcCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCcgcgc                          <  2:4175934/150‑1 (MQ=255)

                                                                                                                                gcCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGa                      <  1:903118/150‑1 (MQ=255)

                                                                                                                                gcCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGa                      >  2:721987/1‑150 (MQ=255)

                                                                                                                                 ccGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAg                     <  1:2361935/150‑1 (MQ=255)

                                                                                                                                     aTCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAgg                 <  2:4082357/150‑1 (MQ=255)

                                                                                                                                      tCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGa                >  2:404667/1‑150 (MQ=255)

                                                                                                                                      tCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGa                >  2:3736038/1‑150 (MQ=255)

                                                                                                                                      tCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGa                >  2:2916399/1‑150 (MQ=255)

                                                                                                                                        cctggctGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGAcc              >  1:437429/7‑150 (MQ=255)

                                                                                                                                        aGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGAcc              <  1:790230/150‑1 (MQ=255)

                                                                                                                                         ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGc                                                                                                                    <  1:491257/47‑1 (MQ=255)

                                                                                                                                         ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGc                                                                                                                    >  2:491342/1‑47 (MQ=255)

                                                                                                                                         ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCATCCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCg             >  2:3757543/1‑150 (MQ=255)

                                                                                                                                         ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCg             >  2:1351448/1‑150 (MQ=255)

                                                                                                                                         ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCg             >  1:2118676/1‑150 (MQ=255)

                                                                                                                                         ctggctGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCg             >  2:2098333/6‑150 (MQ=255)

                                                                                                                                              tGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCg        >  2:4639885/1‑150 (MQ=255)

                                                                                                                                               gcgcCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGt       <  2:326876/150‑1 (MQ=255)

                                                                                                                                                 gcCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGa     >  1:2185943/1‑150 (MQ=255)

                                                                                                                                                 gcCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGa     >  1:3648060/1‑150 (MQ=255)

                                                                                                                                                 gcCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGa     >  1:530615/1‑150 (MQ=255)

                                                                                                                                                    tGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGATCa  <  2:3559951/150‑1 (MQ=255)

GACCGCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGATCA  >  NC_002947/5121016‑5121313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`