Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,127,342 T→G 5.8% G55G (GGT→GGG) ‡ PP_4512 → DMT superfamily permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,127,3420TG 5.8% 596.3 / 17.5 183G55G (GGT→GGG) ‡PP_4512DMT superfamily permease
Reads supporting (aligned to +/- strand):  ref base T (83/89);  new base G (5/6);  total (88/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

GCCCTCGGCGCACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCAGCTTCCCGGCCT  >  NC_002947/5127193‑5127490
                                                                                                                                                     |                                                                                                                                                    
gcCCTCGGCGCACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGt                                                                                                                                                      >  2:4561886/1‑150 (MQ=255)
    tCGGCGCACTGCACCTTGGCGCGCGGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCCGCGCCAGCCCGGCGATCATCGTCGTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCgg                                                                                                                                                  <  1:4147079/150‑1 (MQ=255)
    tCGGCGCACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCgg                                                                                                                                                  >  2:2103229/1‑150 (MQ=255)
    tCGGCGCACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCgg                                                                                                                                                  <  1:1627191/150‑1 (MQ=255)
         gcACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCCGGACCGGCCAGGCTgg                                                                                                                                             <  1:5154194/150‑1 (MQ=255)
         gcACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCCGGACCGGCCAGGCTgg                                                                                                                                             <  2:1609131/150‑1 (MQ=255)
          cACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCCGGACCGGCCAGGCTGGc                                                                                                                                            <  2:2059860/150‑1 (MQ=255)
               cacaTTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTg                                                                                                                                              <  1:573615/143‑1 (MQ=255)
               cacaTTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTg                                                                                                                                              >  2:573615/1‑143 (MQ=255)
                 caTTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTTGCCAGCCCGGACCGGCCAGGCTGGcgaacagg                                                                                                                                     <  2:1263565/150‑8 (MQ=255)
                 caTTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCt                                                                                                                                     <  2:2800691/150‑1 (MQ=255)
                 caTTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCt                                                                                                                                     <  2:623701/150‑1 (MQ=255)
                 caTTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCCGGACCGGCCAGGCTGGcgaacagg                                                                                                                                     <  1:3445353/150‑8 (MQ=255)
                   ttGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGa                                                                                                                                   >  1:4658752/1‑150 (MQ=255)
                      gcgcgcTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGagca                                                                                                                                <  2:1747379/150‑1 (MQ=255)
                      gcgcgcGGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGagca                                                                                                                                <  2:824907/150‑1 (MQ=255)
                        gcgcTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGagcagc                                                                                                                              >  2:5008272/1‑150 (MQ=255)
                           cgggtcgtcGGCCTGACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAg                                                                                                                           <  2:1241513/143‑1 (MQ=255)
                            tGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAgg                                                                                                                          >  1:3847977/1‑150 (MQ=255)
                                cttcGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATgc                                                                                                                      <  1:784774/150‑1 (MQ=255)
                                cttcGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATgc                                                                                                                      <  2:3171093/150‑1 (MQ=255)
                                  tcGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATgcgc                                                                                                                    <  1:4691651/150‑1 (MQ=255)
                                    ggCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCCACGCCTGAGCAGCCAGGATgcgcgc                                                                                                                  >  1:4639724/1‑150 (MQ=255)
                                        ttACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTgg                                                                                                                                             <  2:2718516/119‑1 (MQ=255)
                                        ttACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCgccgcc                                                                                                              >  2:4573087/1‑150 (MQ=255)
                                           ccGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCctgc                                                                                                           <  2:2475528/150‑1 (MQ=255)
                                              gCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCtgctgc                                                                                                        <  1:1222360/150‑1 (MQ=255)
                                              gCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCtgctgc                                                                                                        >  2:3943004/1‑150 (MQ=255)
                                               cGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTgctgct                                                                                                       <  1:1662553/150‑1 (MQ=255)
                                               cGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTgctgct                                                                                                       <  1:1662434/150‑1 (MQ=255)
                                                  cTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCgg                                                                                                    <  2:483792/150‑1 (MQ=255)
                                                   ttCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCACCGCGTGAGCAGCGAGGATGCGCGCCGCCTGCTGCTCggt                                                                                                   >  1:2354914/1‑150 (MQ=255)
                                                   ttCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCggt                                                                                                   <  1:400457/150‑1 (MQ=255)
                                                   ttCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCggt                                                                                                   <  2:3621239/150‑1 (MQ=255)
                                                     cGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCggtgg                                                                                                 >  1:1545967/1‑150 (MQ=255)
                                                      ggCAAACTGGCCACCATCGCCAGCCCGGCGATCATCTTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGACGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCggtggt                                                                                                >  2:2478347/1‑150 (MQ=255)
                                                      ggCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCggtggt                                                                                                >  1:673617/1‑150 (MQ=255)
                                                       gCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGgtggtg                                                                                               >  2:5354674/1‑150 (MQ=255)
                                                       gCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGgtggtg                                                                                               <  1:3188431/150‑1 (MQ=255)
                                                       gCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGgtggtg                                                                                               >  1:4801057/1‑150 (MQ=255)
                                                               gCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGctgctg                                                                                       <  1:2796961/150‑1 (MQ=255)
                                                                 caccaGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggc                                                                                     >  1:4326706/1‑150 (MQ=255)
                                                                 caccaGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggc                                                                                     >  1:5441914/1‑150 (MQ=255)
                                                                  accaGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggcc                                                                                    <  2:673608/150‑1 (MQ=255)
                                                                   ccaGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggccg                                                                                   <  1:4388886/150‑1 (MQ=255)
                                                                     aGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggccggc                                                                                 <  2:2943734/150‑1 (MQ=255)
                                                                     aGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggccggc                                                                                 >  2:1896478/1‑150 (MQ=255)
                                                                      gcgcCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggccggcc                                                                                >  1:3824617/1‑150 (MQ=255)
                                                                           aGCCCGGCGATCATCGTCTTCGGTCGCGCCAGCTTCGGCGTACTCGCGCTGGCCGTGTTCGCCAGCCTGGCCGGTCCGGGCTTGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTgg                                                                           <  1:3933261/150‑1 (MQ=255)
                                                                           aGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTgg                                                                           <  1:2728879/150‑1 (MQ=255)
                                                                            gCCCGGCGATCATTGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGACTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTggg                                                                          >  2:3825934/1‑150 (MQ=255)
                                                                              ccGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTc                                                                        <  1:3627329/150‑1 (MQ=255)
                                                                                   gATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTg                                                                            <  2:2236855/141‑1 (MQ=255)
                                                                                    atcatcGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGcttc                                                                  <  1:3796732/150‑1 (MQ=255)
                                                                                    atcatcGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGACAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGcttc                                                                  <  1:3161571/150‑1 (MQ=255)
                                                                                      catcGTCTTCGGTCGCGCCACCGTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGcttctt                                                                <  1:459194/150‑1 (MQ=255)
                                                                                       atcGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCttcttc                                                               >  1:3365788/1‑150 (MQ=255)
                                                                                        tcGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCa                                                              >  1:1394532/1‑150 (MQ=255)
                                                                                          gTCTTCGGTCGCGCCACGTTCGCCGTACTCGCGCTGGCCGTGTTCGCCAGCCTGGCCGGTCCGGGCTGGGAACGCCTGTGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTTCTGCTGGCAGGCCACTGTGTCAGCTTCTTGATc                                                            <  2:5267655/150‑1 (MQ=255)
                                                                                          gTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATc                                                            <  1:127439/150‑1 (MQ=255)
                                                                                           tCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCg                                                           >  1:3241148/1‑150 (MQ=255)
                                                                                            cTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGc                                                          <  2:3288191/150‑1 (MQ=255)
                                                                                             ttCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGcc                                                         >  1:96001/1‑150 (MQ=255)
                                                                                              tCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCg                                                        >  2:2337335/1‑150 (MQ=255)
                                                                                               cGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTggccggc                                                                                 <  2:4625078/124‑1 (MQ=255)
                                                                                                ggTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTc                                                      <  2:3129937/150‑1 (MQ=255)
                                                                                                   cgcgCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCaaa                                                   <  1:2326185/150‑1 (MQ=255)
                                                                                                     cgcCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGt                                                 >  2:4825891/1‑150 (MQ=255)
                                                                                                      gcCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTg                                                >  2:2981006/1‑150 (MQ=255)
                                                                                                       ccACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTgg                                               <  2:3566079/150‑1 (MQ=255)
                                                                                                          ccTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGggcg                                            <  1:1784350/150‑1 (MQ=255)
                                                                                                          ccTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGggcg                                            <  1:2550395/150‑1 (MQ=255)
                                                                                                               gCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGgcggcgat                                       <  2:746508/150‑3 (MQ=255)
                                                                                                               gCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTa                                       <  2:5011897/150‑1 (MQ=255)
                                                                                                               gCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTa                                       <  2:5011985/150‑1 (MQ=255)
                                                                                                               gCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTa                                       <  1:610746/150‑1 (MQ=255)
                                                                                                                   tACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCa                                   <  2:1434750/150‑1 (MQ=255)
                                                                                                                     cTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATc                                 <  1:5008272/150‑1 (MQ=255)
                                                                                                                      tCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGtgc                                                                                            <  1:2447534/90‑1 (MQ=255)
                                                                                                                      tCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCg                                <  2:938603/150‑1 (MQ=255)
                                                                                                                      tCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCg                                >  2:2166275/1‑150 (MQ=255)
                                                                                                                        gcgcTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTgctgct                                                                                        >  2:1997075/1‑92 (MQ=255)
                                                                                                                        gcgcTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTgctgct                                                                                        <  1:1997075/92‑1 (MQ=255)
                                                                                                                        gcgcTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGcc                              >  2:4283864/1‑150 (MQ=255)
                                                                                                                            tGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCAccc                          >  1:4182898/1‑150 (MQ=255)
                                                                                                                                 cTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGc                     >  2:3525147/1‑150 (MQ=255)
                                                                                                                                   gTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCtt                   <  1:2064514/150‑1 (MQ=255)
                                                                                                                                    ttCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTc                  <  1:416685/150‑1 (MQ=255)
                                                                                                                                    gttgCCAGCCCGGACCGGCCAGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTc                  >  1:4081977/4‑150 (MQ=255)
                                                                                                                                     ttgCCAGCCCGGACCGGCCAGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCg                 >  2:4466984/3‑150 (MQ=255)
                                                                                                                                      cGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGc                <  2:4322956/150‑1 (MQ=255)
                                                                                                                                       gCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCATCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGcc               <  2:4207446/150‑1 (MQ=255)
                                                                                                                                        ccAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCa              >  1:2700138/1‑150 (MQ=255)
                                                                                                                                        ccAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCa              <  2:4338619/150‑1 (MQ=255)
                                                                                                                                        ccAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCa              >  1:100327/1‑150 (MQ=255)
                                                                                                                                         cAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCAg             >  2:3126022/1‑150 (MQ=255)
                                                                                                                                          aGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCAGc            >  1:5426485/1‑150 (MQ=255)
                                                                                                                                              tGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCg                                <  2:4730680/126‑1 (MQ=255)
                                                                                                                                                gCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCAGCTTCCCg      >  2:3855435/1‑150 (MQ=255)
                                                                                                                                                    gTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCAGCTTCCCGGCCt  >  1:5196746/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCCTCGGCGCACTGCACATTGGCGCGCTGTTCTTCGGCCTTACCGGCGTCTTCGGCAAACTGGCCACCAGCGCCAGCCCGGCGATCATCGTCTTCGGTCGCGCCACCTTCGCCGTACTCGCGCTGGCCCTGTTCGCCAGCCTGGCCGGTCCGGGCTGGCAACGCCTGAGCAGCCAGGATGCGCGCCGCCTGCTGCTCGGTGGTGTGCTGCTGGCCGGCCACTGGGTCAGCTTCTTCATCGCCGTCAAAGTGGGCGGCGTAGCCATCGCCACCCTCGGCTTCGCCAGCTTCCCGGCCT  >  NC_002947/5127193‑5127490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑