Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,184,035 T→G 18.9% intergenic (‑123/+31) PP_4560 ← / ← csbD ribonuclease BN/stress response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,184,0350TG18.9% 261.2 / 67.8 138intergenic (‑123/+31)PP_4560/csbDribonuclease BN/stress response protein
Reads supporting (aligned to +/- strand):  ref base T (58/49);  new base G (13/12);  total (71/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-02

Only 100 of 139 total aligned reads displayed.

CAAGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACA  >  NC_002947/5183886‑5184180
                                                                                                                                                     |                                                                                                                                                 
cAAGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGtt                                                                                                                                                   <  2:4558098/150‑1 (MQ=255)
 aaGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTc                                                                                                                                                  >  1:2960014/1‑150 (MQ=255)
  aGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCa                                                                                                                                                 >  1:4997366/1‑150 (MQ=255)
     cgcgCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAccc                                                                                                                                              <  1:1689608/150‑1 (MQ=255)
       cgcAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCga                                                                                                                                            <  1:1992092/150‑1 (MQ=255)
         cAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCgcga                                                                                                                                          <  2:2624914/150‑1 (MQ=255)
         cAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCgaga                                                                                                                                          <  1:5124246/150‑1 (MQ=255)
          aGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGTCCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaa                                                                                                                                         <  2:4150006/150‑1 (MQ=255)
          aGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaa                                                                                                                                         <  2:3817328/150‑1 (MQ=255)
          aGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaa                                                                                                                                         <  2:4022513/150‑1 (MQ=255)
          aGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaa                                                                                                                                         <  2:2914017/150‑1 (MQ=255)
           ggTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaag                                                                                                                                        <  2:2670932/150‑1 (MQ=255)
            gTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                       <  2:917607/150‑1 (MQ=255)
            gTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                       <  1:490120/150‑1 (MQ=255)
            gTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                       <  1:1780209/150‑1 (MQ=255)
            gTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaaga                                                                                                                                       <  1:4687692/150‑1 (MQ=255)
             tCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                       <  2:2328633/149‑1 (MQ=255)
             tCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                       >  1:2328633/1‑149 (MQ=255)
             tCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                                      <  1:4060320/150‑1 (MQ=255)
              cGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAgg                                                                                                                                     <  1:4522397/150‑1 (MQ=255)
                  gAAAATCATAGCGTCTCCTTTCGCCGCCTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGcc                                                                                                                                   <  2:232143/148‑1 (MQ=255)
                  gAAAATCATAGCGTCTCCTTTCGCCGCCTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGcc                                                                                                                                   >  1:232143/1‑148 (MQ=255)
                   aaaaTCATAGCGGCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGc                                                                                                                                <  2:3657517/150‑1 (MQ=255)
                        caccgCGTCCCCTTTCGCCGCTTGACCGGTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTTAAGAATTTGCGTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCAGCACCGc                                                                                                                           <  1:3896612/146‑1 (MQ=255)
                           tgcttctcCTTTGGCGGCTTGTTCGTTCCACTGGCAAGTCTATTAGACGGGGCCCTGGTTGTGCTGCTCCCGGCCGTGTTTTAATATTTTGATTACGAATACTTGCCGGCCTGTGCGGGGGTCCACCCGAGACGAGGCCGGCACCGCCtc                                                                                                                        <  2:254375/146‑1 (MQ=255)
                            gggTCTCCTTGCGCCGCTTGATCGTTACACTGGAAAGTCTAATCGACCGTGCCCGCGCTGTGCTGCTCCCGGCGGTGTTTGAAGATTTTGGTTCGGAATACGTGCCGGCCTGTTCGGGGGTTCACCGGAGACGCGGCCGGCACCGCCtca                                                                                                                       <  2:753811/148‑1 (MQ=255)
                             cGTCGCCTTTCGCCGCGTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGACCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaa                                                                                                                      <  2:4786486/150‑1 (MQ=255)
                              gTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaa                                                                                                                                         >  2:4783058/1‑130 (MQ=255)
                                 tcCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtca                                                                                                                  >  1:1345660/1‑150 (MQ=255)
                                   cTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaagtcaag                                                                                                                >  2:4459207/1‑150 (MQ=255)
                                    tttCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAgg                                                                                                               >  2:2062741/1‑150 (MQ=255)
                                    tttCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAgg                                                                                                               >  2:4808027/1‑150 (MQ=255)
                                           gcTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGCTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCttcttc                                                                                                        >  2:1796398/1‑150 (MQ=255)
                                             ttGATCGTTACACCGGCACGTCTATTAGACCGTGCCCGAGGTGTGCTGCTCCCGGCGTTGGTTTATGATTTTGGTTACCATTACGTGCGGGCCTCGTGGCGGGTTCCCCCGTGATGCGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAc                                                                                                      <  2:4598930/150‑1 (MQ=255)
                                               gATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCg                                                                                                    >  1:4712303/1‑150 (MQ=255)
                                                    ttACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATcc                                                                                               >  1:1958462/1‑150 (MQ=255)
                                                      acacAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCtt                                                                                             >  1:4976352/1‑150 (MQ=255)
                                                        acaGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTca                                                                                           >  2:3143549/1‑150 (MQ=255)
                                                        acaGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTca                                                                                           >  1:1454568/1‑150 (MQ=255)
                                                          aGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTcaca                                                                                         >  2:1177646/1‑150 (MQ=255)
                                                          aGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTcaca                                                                                         >  1:4480478/1‑150 (MQ=255)
                                                          aGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTcaca                                                                                         >  1:4480381/1‑150 (MQ=255)
                                                          aGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTcaca                                                                                         >  2:4047288/1‑150 (MQ=255)
                                                                 tCTAATTGCCGGGGCCCGTGCTGTGGGGCGCCCGGCGGTGTTTCAATATTTTGCTTACTAATACGGGCCGGCCTCTGCGGGGTTCCCCCCGAGACGCGCCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCt                                                                                  <  1:3058549/150‑1 (MQ=255)
                                                                   tAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                                      >  2:1327790/1‑96 (MQ=255)
                                                                   tAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCACATCCTTCACATTTCCCTTg                                                                                >  1:4094520/1‑150 (MQ=255)
                                                                        gACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACCTTTCCCTTGACctg                                                                           >  1:487191/1‑150 (MQ=255)
                                                                          ccGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACctgct                                                                         >  2:2959899/1‑150 (MQ=255)
                                                                           cGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACctgctg                                                                        >  2:1328635/1‑150 (MQ=255)
                                                                               cccGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGcgcc                                                                    >  1:4649005/1‑150 (MQ=255)
                                                                                  gAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGcgcctcg                                                                 >  1:1088512/1‑150 (MQ=255)
                                                                                       gtgCTGCTCCCGGCCGGGGTTGATGAATGTGCTTACGAATACGGGCCGGCCGCGGCGCGGGTTCACCCGAGACGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCttt                                                            <  2:3718344/150‑1 (MQ=255)
                                                                                         gctgcgCCCGGCGGTGGTTTAAGATTTTGCTTACGAATATGGGCCGGCCACTTCGCGGGTCCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGa                                                          <  1:3969799/150‑1 (MQ=255)
                                                                                         gcggctCCGGGCGGTGTTTTATTATTTTGCTTACGAATAGGGGCGGGCCGCTGCGGGGGTACCCCCGCGACGGGCCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCACCTCGCCTTTGa                                                          <  1:2832502/147‑1 (MQ=255)
                                                                                           tgctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                       >  1:2695047/1‑71 (MQ=25)
                                                                                             ctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTc                                                      >  2:4635522/1‑150 (MQ=255)
                                                                                              tCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTcc                                                     >  1:2772888/1‑150 (MQ=255)
                                                                                                 gggCGGTGGTTTATGATTTTGCTTACTAATACGTGCGGGCCTCTGCGCGGGTACACCCGCTAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTgc                                                  <  2:1919672/149‑1 (MQ=255)
                                                                                                      gTGGTTGAATAATTTGCTTACGAATACGGGCCGGCCTCTTCGCGGGTTCACCCGAGAAGTGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgcctt                                             <  2:772212/150‑1 (MQ=255)
                                                                                                       gggTTGACTATTTTGTTTACTAATATGGGCCGGCCTCTGCGGGGGTTCCCCGGTGACGCGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttg                                            <  1:834183/149‑1 (MQ=255)
                                                                                                         gTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcc                                          >  1:2697419/1‑150 (MQ=255)
                                                                                                            gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTc                                       >  1:168633/1‑150 (MQ=255)
                                                                                                             atgaaTTTGCTTACTAATAAGTGCCGGCCTCTTCGGGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCg                                      <  1:5365646/148‑1 (MQ=255)
                                                                                                             aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCg                                      <  1:5365584/150‑1 (MQ=255)
                                                                                                             aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCg                                      <  2:2697419/150‑1 (MQ=255)
                                                                                                               tatttggCTTACTATTATGGGCGGCCCTCTCCGGGGGTACCCCCGCGCAGCGCCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGc                                    <  1:4593144/144‑1 (MQ=255)
                                                                                                                    tGCTTACGAATAAGTGCCGGCCTCTGCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCa                               <  2:5283361/150‑1 (MQ=255)
                                                                                                                       ttACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCt                            >  1:4902243/1‑150 (MQ=255)
                                                                                                                       ttACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACAGTTCCCTTGACCTGCTGCGCCTCGCCTGTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGcg                            >  2:2757948/1‑149 (MQ=255)
                                                                                                                        tACGAATCCGTGCCGGCCCCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtt                           <  2:2276091/150‑1 (MQ=255)
                                                                                                                         aCGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCttt                          >  2:5233678/1‑150 (MQ=255)
                                                                                                                           gAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTc                        <  2:4477240/150‑1 (MQ=255)
                                                                                                                               aaGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTg                    >  1:4158136/1‑150 (MQ=255)
                                                                                                                                aGTGCCGGCCTCTTCGCGGGTTCACCCGAGACGGGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt                   <  1:3463353/150‑1 (MQ=255)
                                                                                                                                 gTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTc                  >  1:1288354/1‑150 (MQ=255)
                                                                                                                                 gTGCCGGCCTCTGCGCGGGTTCACCCGAGAAGAGCCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTc                  <  1:4863494/150‑1 (MQ=255)
                                                                                                                                  tGCCGGCCTCTTCGGGGGTTCACCCGAGAAGCGCCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                 <  1:4742195/150‑1 (MQ=255)
                                                                                                                                  tGCCGGCCTCTTCGGGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                 <  1:3156722/150‑1 (MQ=255)
                                                                                                                                     cGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTc              >  1:1611678/1‑150 (MQ=255)
                                                                                                                                     cGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTc              >  1:1611367/1‑150 (MQ=255)
                                                                                                                                      ggCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCa             >  1:2238984/1‑150 (MQ=255)
                                                                                                                                        ccTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCAcc           >  1:3559370/1‑150 (MQ=255)
                                                                                                                                         ctctTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                 <  2:3453628/143‑1 (MQ=255)
                                                                                                                                         ctctTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCg                 >  1:3453628/1‑143 (MQ=255)
                                                                                                                                         ctctTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCt          >  1:2727744/1‑150 (MQ=255)
                                                                                                                                         ctctTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTGTTCGTTGTCGGTCACCt          >  1:5308298/1‑150 (MQ=255)
                                                                                                                                          tcttctCGGGTGAACCCGCGAAGAGGCCGGCACTGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt         >  2:3506541/1‑150 (MQ=255)
                                                                                                                                          tctTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTc                        >  1:4970286/1‑135 (MQ=255)
                                                                                                                                          tctTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTc                        <  2:4970286/135‑1 (MQ=255)
                                                                                                                                            ttCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTc                                                                                            >  1:840347/1‑65 (MQ=21)
                                                                                                                                            ttCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTc                                                                                            <  2:840347/65‑1 (MQ=21)
                                                                                                                                            ttCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGc       >  2:4243836/1‑150 (MQ=255)
                                                                                                                                            ttCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGc       >  2:3549476/1‑150 (MQ=255)
                                                                                                                                            ttCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGc       >  1:3499267/1‑150 (MQ=255)
                                                                                                                                            ttCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGc       >  1:1366565/1‑150 (MQ=255)
                                                                                                                                             tCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGcc      >  1:1313779/1‑150 (MQ=255)
                                                                                                                                               gcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTcc                                                                                    <  1:5261153/70‑1 (MQ=255)
                                                                                                                                               gcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCaa    <  2:1611367/150‑1 (MQ=255)
                                                                                                                                               gcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCaa    <  2:1611678/150‑1 (MQ=255)
                                                                                                                                                 gggTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAaca  >  1:3714684/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CAAGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACA  >  NC_002947/5183886‑5184180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑