BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	311,771	A→G	10.6%	L203L (TTG→CTG) ‡	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	311,771	0	A	G	10.6%	557.9 / 41.3	198	L203L (TTG→CTG) ‡	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (73/104); new base G (6/15); total (79/119)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01

Only 100 of 215 total aligned reads displayed.

CTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTGGCCAGCT  >  NC_002947/311622‑311920

cTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCaa                                                                                                                                                       <  1:903978/150‑1 (MQ=255)

 tGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAac                                                                                                                                                      <  2:63729/150‑1 (MQ=255)

     tgcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgcca                                                                                                                                                  <  2:2201134/149‑1 (MQ=255)

      gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACCAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCCacgccac                                                                                                                                                 >  2:4342832/1‑150 (MQ=255)

      gcCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccac                                                                                                                                                 >  2:1879979/1‑150 (MQ=255)

       ccACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacg                                                                                                                                                >  2:830706/1‑150 (MQ=255)

        cACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgc                                                                                                                                               >  2:5347513/1‑150 (MQ=255)

         aCGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAacgccacgcc                                                                                                                                              >  1:3475558/1‑150 (MQ=255)

            cTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCt                                                                                                                                           <  2:3342901/150‑1 (MQ=255)

             tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                                                          >  1:4862958/1‑150 (MQ=255)

             tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                                                          <  1:5111275/150‑1 (MQ=255)

             tttACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTg                                                                                                                                          <  2:1284917/150‑1 (MQ=255)

               tACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTAGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGc                                                                                                                                        <  2:3829389/150‑1 (MQ=255)

                aCCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGcc                                                                                                                                       >  1:1766072/1‑150 (MQ=255)

                   gCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCa                                                                                                                                    >  2:2858569/1‑150 (MQ=255)

                    cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                                                                                                   <  2:496085/150‑1 (MQ=255)

                    cATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCAt                                                                                                                                   >  1:284610/1‑150 (MQ=255)

                      tCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                                                                 >  1:4394451/1‑150 (MQ=255)

                       cACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGc                                                                                                                                <  1:673100/150‑1 (MQ=255)

                       cACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGc                                                                                                                                >  2:3953223/1‑150 (MQ=255)

                             gTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGt                                                                                                                          >  1:412278/1‑150 (MQ=255)

                               gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                                                        <  2:4862958/150‑1 (MQ=255)

                               gggTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                                                        <  2:3603045/150‑1 (MQ=255)

                                 gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                                                                                      <  1:611892/150‑1 (MQ=255)

                                 gTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGa                                                                                                                      <  1:1185687/150‑1 (MQ=255)

                                  cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                                                                                                     <  2:3857120/145‑2 (MQ=255)

                                  cccaacATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGaa                                                                                                                     <  2:2088191/145‑2 (MQ=255)

                                    gAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCa                                                                                                                   >  1:4515812/1‑150 (MQ=255)

                                      gCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGt                                                                                                                 <  1:5361846/150‑1 (MQ=255)

                                      acATGCGGGGATGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGGTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGt                                                                                                                 <  2:4781816/149‑1 (MQ=255)

                                        aTGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCa                                                                                                               <  2:1680453/150‑1 (MQ=255)

                                           cgcgGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAggg                                                                                                            <  2:2399080/150‑1 (MQ=255)

                                            gcgGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGa                                                                                                           >  1:5320372/1‑150 (MQ=255)

                                              ggatGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGaaa                                                                                                         <  1:2939829/147‑2 (MQ=255)

                                               ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                                                                 >  2:2296565/1‑125 (MQ=255)

                                               ggTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATgg                                                                                                                                 <  1:2296565/125‑1 (MQ=255)

                                                atGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAatt                                                                                                       <  2:1112865/149‑1 (MQ=255)

                                                atGATTGGTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAatt                                                                                                       <  1:2798487/149‑1 (MQ=255)

                                                  gATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgttgt                                                                                                     <  2:1913405/150‑1 (MQ=255)

                                                    ttGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGttgttg                                                                                                   <  1:379824/150‑1 (MQ=255)

                                                     tGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTc                                                                                                                        >  1:187281/1‑128 (MQ=255)

                                                       gTGCCAGGATTTTGCCCCAC‑GACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTg                                                                                                >  1:3339559/1‑150 (MQ=255)

                                                         gCCGGGGTTCCGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGcc                                                                                              <  2:3147046/150‑1 (MQ=255)

                                                           cGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGt                                                                                            <  1:2676809/150‑1 (MQ=255)

                                                             gggTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg                                                                                          >  1:1333434/1‑150 (MQ=255)

                                                             gggTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAg                                                                                          >  1:1096766/1‑150 (MQ=255)

                                                                  tttgCCCCACGACGAAAATCGCGTCAGCCTGCTCGAAATCCTC‑AAGCAGAACAGTTCCCTTGCCAACCCCTACCGATTGTTTGAGCGCCACACCGCTGGCCTCATGGCACATGTTCGAACAGTCAGGGAAATTGTTCGTGCCATAAgcgc                                                                                      <  1:402085/147‑1 (MQ=255)

                                                                   tGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGTCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGgcgcgc                                                                                    >  2:3096652/1‑150 (MQ=255)

                                                                    ggCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCa                                                                                   >  1:3503488/1‑150 (MQ=255)

                                                                      ccccA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCAcgccgc                                                                                                                                            <  2:3796016/91‑1 (MQ=255)

                                                                         cA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAAc                                                                              <  2:1494475/150‑1 (MQ=255)

                                                                             aCAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGt                                                                           >  1:2511125/1‑150 (MQ=255)

                                                                              cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                                                                          >  2:1315288/1‑150 (MQ=255)

                                                                              cAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGtt                                                                          <  1:3196537/150‑1 (MQ=255)

                                                                                aaaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTgg                                                                        <  2:4394451/150‑1 (MQ=255)

                                                                                aaaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTgg                                                                        <  2:1092420/150‑1 (MQ=255)

                                                                                 aaaTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGt                                                                       <  2:524700/150‑1 (MQ=255)

                                                                                   aTGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTAc                                                                     <  1:3163121/150‑1 (MQ=255)

                                                                                       cGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGt                                                                 <  1:4568972/150‑1 (MQ=255)

                                                                                        gTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTa                                                                >  1:4706220/1‑150 (MQ=255)

                                                                                            gCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTAcgcc                                                            <  1:3731415/150‑1 (MQ=255)

                                                                                                tcTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCtt                                                        <  2:2716548/149‑1 (MQ=255)

                                                                                                 cTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTc                                                       <  2:3456951/150‑1 (MQ=255)

                                                                                                  tCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCg                                                      >  2:1109370/1‑150 (MQ=255)

                                                                                                  tCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCg                                                      <  2:3241060/150‑1 (MQ=255)

                                                                                                    gAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGtt                                                    >  1:2081504/1‑150 (MQ=255)

                                                                                                      aGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGc                                                  <  1:1265063/150‑1 (MQ=255)

                                                                                                       gtcgtcGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCt                                                 <  2:1208242/150‑1 (MQ=255)

                                                                                                        tcgtcgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTcacccagc                                                <  1:4265612/150‑8 (MQ=255)

                                                                                                        tcgtcgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTg                                                <  1:3271511/150‑1 (MQ=255)

                                                                                                            cgAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCAc                                            <  1:475047/150‑1 (MQ=255)

                                                                                                                ggTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTcc                                        >  2:4347772/1‑150 (MQ=255)

                                                                                                                 atG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTccc                                       <  1:3788868/149‑1 (MQ=255)

                                                                                                                   g‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGa                                     <  1:150501/150‑1 (MQ=255)

                                                                                                                   g‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGa                                     <  2:1714586/150‑1 (MQ=255)

                                                                                                                   g‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGa                                     <  2:4515812/150‑1 (MQ=255)

                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAg                                    <  1:2170066/150‑1 (MQ=255)

                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAg                                    <  1:2031952/150‑1 (MQ=255)

                                                                                                                     aCTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTAATGGCTCATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCgtg                                    <  1:5406883/150‑3 (MQ=255)

                                                                                                                       tGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGt                                  >  2:723154/1‑150 (MQ=255)

                                                                                                                         tACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGa                                     <  2:654267/145‑1 (MQ=255)

                                                                                                                             tttGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGaa                            <  1:1826932/150‑1 (MQ=255)

                                                                                                                                  cGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCgg                       <  1:3953223/150‑1 (MQ=255)

                                                                                                                                   gACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCGCATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGc                      >  2:1799777/1‑150 (MQ=255)

                                                                                                                                   gACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGc                      <  1:537552/150‑1 (MQ=255)

                                                                                                                                   gACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGc                      >  2:4157843/1‑150 (MQ=255)

                                                                                                                                       ccGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGc                                              >  1:4212444/1‑122 (MQ=255)

                                                                                                                                        cGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGt                 >  1:4364285/1‑150 (MQ=255)

                                                                                                                                         gACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGCAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTc                >  1:2244633/1‑150 (MQ=255)

                                                                                                                                            gCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAgg             <  1:4342832/150‑1 (MQ=255)

                                                                                                                                               ttGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGtt          >  1:820531/1‑150 (MQ=255)

                                                                                                                                                tGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTg         <  1:3384574/150‑1 (MQ=255)

                                                                                                                                                tGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGAAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTg         <  1:1879979/150‑1 (MQ=255)

                                                                                                                                                  ccccAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTGGc       <  1:2659731/150‑1 (MQ=255)

                                                                                                                                                    ccAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgtt                                                                                                       <  2:3640003/52‑1 (MQ=255)

                                                                                                                                                    ccAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAgtt                                                                                                       >  1:3640003/1‑52 (MQ=255)

                                                                                                                                                    ccAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTGGCCa     <  2:1338981/150‑1 (MQ=255)

                                                                                                                                                     cAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTCTAGAATTCGGCCTGGTCAGGGGTGGCCAg    >  1:4961667/1‑150 (MQ=255)

                                                                                                                                                      aaCGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTGGCCAGc   >  1:3755972/1‑150 (MQ=255)

                                                                                                                                                       acgccacgccGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTGGCCAGCt  <  2:1766072/150‑1 (MQ=255)

CTGGGCGCCACGCTTTACCGCATCACGCAGTGGGTCGAGCATGCGCGGGTGATTGGTGCCGGGGTTCTGGCCCCA‑GACAAAAATGGCGTCGGCGTGCTCGAAGTCGTCGAAGGTG‑ACTGTACCTTTGCCGACCCCGACGCTTTGCCCCAACGCCACGCCGCTGGCCTCATGGCACATGTTCGAGCAGTCAGGGAAGTTGTTGGTGCCGTAGGCGCGCACGAACAGTTGGTACAGGTACGCCGCTTCGTTGCTGGCACGTCCCGAGGTATAGAATTCGGCCTGGTCAGGGTTGGCCAGCT  >  NC_002947/311622‑311920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`