| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,304,631 | T→A | 7.7% | E371V (GAG→GTG) ‡ | recB ← | Chi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,304,631 | 0 | T | A | 7.7% | 461.1 / 19.6 | 142 | E371V (GAG→GTG) ‡ | recB | Chi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease |
| Reads supporting (aligned to +/- strand): ref base T (85/46); new base A (2/9); total (87/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.32e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 144 total aligned reads displayed.
GAACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTTGC > NC_002947/5304486‑5304780 | gAACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCt > 2:2588440/1‑150 (MQ=255)gAACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCt > 2:3914921/1‑150 (MQ=255) aaCAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTg > 2:5140871/1‑150 (MQ=255) aCAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTgg > 2:911146/1‑150 (MQ=255) cAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGc < 2:493126/150‑1 (MQ=255) aCCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCAGCGAGGCCGGCGAg < 2:5101173/150‑1 (MQ=255) ccGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGCTCGCCAGCGAGGCCGGCGAgc < 2:1331790/150‑1 (MQ=255) ccGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGCTCGCCAGCGAGGCCGGCGAgc < 2:1331815/150‑1 (MQ=255) ttCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtg < 1:2226524/150‑1 (MQ=255) ttCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtg > 1:2611168/1‑150 (MQ=255) ttCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGCTCGCCAGCGAGGCCGGCGAGCGCCtg < 2:3113457/150‑1 (MQ=255) tGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtgctg > 1:4590797/1‑150 (MQ=255) gcgcGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTAACGGATCAGGCTGGCCAGGCGCTCGCCAGCGAGGCCGGCGAGCGCCtggcca < 2:4366514/150‑5 (MQ=255) gcgcGATTTTCGCTGATCTGGTAGAGGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGtgctgc < 1:3788248/150‑1 (MQ=255) cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCa > 2:4091996/1‑150 (MQ=255) cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCa > 1:5446933/1‑150 (MQ=255) cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGACCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCa > 2:149840/1‑150 (MQ=255) ttttCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAgcgc < 1:446674/150‑1 (MQ=255) cGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGa < 1:4686791/150‑1 (MQ=255) tGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCa > 1:5020659/1‑150 (MQ=255) gATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCAt < 1:3914266/150‑1 (MQ=255) tGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCTCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtcg > 2:593007/1‑150 (MQ=255) tGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtcg > 2:3370191/1‑150 (MQ=255) tGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtcg > 1:2074033/1‑150 (MQ=255) gTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtcgtc > 1:824918/1‑150 (MQ=255) tAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGtcgtcg < 2:4778499/150‑1 (MQ=255) gATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGaa < 2:3911832/150‑1 (MQ=255) gATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGaa < 2:3911888/150‑1 (MQ=255) gATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGaa > 1:691372/1‑150 (MQ=255) tGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGc > 2:2797216/1‑150 (MQ=255) gcTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCa > 1:2845063/1‑150 (MQ=255) gcTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCa > 1:2845004/1‑150 (MQ=255) gcTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCa > 2:2609389/1‑150 (MQ=255) tCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAtt > 1:2391098/1‑150 (MQ=255) tCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAtt > 2:2425285/1‑150 (MQ=255) cGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAttt < 1:888218/150‑1 (MQ=255) aaTGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGc < 1:435394/150‑1 (MQ=255) tGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCAc < 1:214355/150‑1 (MQ=255) tGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGgcgc < 1:957438/150‑1 (MQ=255) tGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGCTCGCCAGCGAGGCCGGCGAGCGCCtg < 2:2301677/101‑1 (MQ=37) tGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGCTCGCCAGCGAGGCCGGCGAGCGCCtg > 1:2301677/1‑101 (MQ=37) aaCCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCtt > 2:4163917/1‑150 (MQ=255) aCCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTtc > 2:4920880/1‑150 (MQ=255) ccGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTtct > 1:2385117/1‑150 (MQ=255) cGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTATCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTtctc > 1:2363003/1‑150 (MQ=255) gTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCAc > 1:1325878/1‑150 (MQ=255) tCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACt < 2:1463375/150‑1 (MQ=255) gtgtCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCg > 1:53814/1‑150 (MQ=255) gtgtCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCg > 1:3993012/1‑150 (MQ=255) tCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCt > 2:3292530/1‑123 (MQ=255) tCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCt < 1:3292530/123‑1 (MQ=255) tCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGaaa > 1:3896253/1‑150 (MQ=255) ntGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAAcg > 1:4452990/2‑150 (MQ=255) cTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAAcg > 2:2517996/1‑150 (MQ=255) gAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACgcgc > 2:4005212/1‑150 (MQ=255) aaTTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGcc < 1:2588099/150‑1 (MQ=255) ttCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGa > 1:2702274/1‑150 (MQ=255) tcgtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGAt < 2:1008213/150‑1 (MQ=255) gtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCgccac > 1:3947066/1‑146 (MQ=255) gtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATAc < 2:4452990/150‑1 (MQ=255) gtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATAc > 2:1976972/1‑150 (MQ=255) gATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCa > 1:3103444/1‑150 (MQ=255) aTCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCaa > 2:3762164/1‑150 (MQ=255) cgcTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggc > 1:3893677/1‑150 (MQ=255) gcTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcg > 1:3155404/1‑150 (MQ=255) gcTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcg < 2:5406066/150‑1 (MQ=255) cTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcgg < 1:1111344/150‑1 (MQ=255) cTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcgg < 1:1111127/150‑1 (MQ=255) tACCGGGAACTGCTCACGGATCAGGCTGGCCGGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcggc > 2:3281756/1‑150 (MQ=255) tACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcggc < 1:5403301/150‑1 (MQ=255) ccGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGt < 1:3370191/150‑1 (MQ=255) cGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTg > 1:808280/1‑150 (MQ=255) aCTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGa < 1:1236861/150‑1 (MQ=255) tGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGagc < 2:197689/150‑1 (MQ=255) tCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGagcagc > 2:1987531/1‑150 (MQ=255) aCGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCgg > 2:4728081/1‑150 (MQ=255) aCGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCgg > 2:356798/1‑150 (MQ=255) aCGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCAACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCgg > 2:2449568/1‑150 (MQ=255) ggATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCgggg < 1:3762164/150‑1 (MQ=255) gCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCgg < 2:5026852/150‑1 (MQ=255) gCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCgg < 1:2797216/150‑1 (MQ=255) gCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCgg > 2:5220400/1‑150 (MQ=255) cTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCggg < 2:2611168/150‑1 (MQ=255) tGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGc > 1:4449109/1‑150 (MQ=255) ccAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGt > 2:2283675/1‑150 (MQ=255) ccAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGt > 2:707070/1‑150 (MQ=255) cAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTc > 1:4679042/1‑150 (MQ=255) gcgcTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCa > 1:63020/1‑86 (MQ=255) gcgcTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCa < 2:63020/86‑1 (MQ=255) gcTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcg > 1:2804596/1‑113 (MQ=255) gcTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcg < 2:2804596/113‑1 (MQ=255) gcTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAggg > 2:3506983/1‑150 (MQ=255) gcTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAggg > 1:4284430/1‑150 (MQ=255) tCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggc > 2:4595219/1‑110 (MQ=255) tCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggc < 1:4595219/110‑1 (MQ=255) cGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCt < 1:1397686/150‑1 (MQ=255) gCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTg > 2:3420766/1‑150 (MQ=255) cTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTtg > 1:983718/1‑150 (MQ=255) tCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTtgc < 1:4920880/150‑1 (MQ=255) tCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACGTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTtgc > 1:648583/1‑150 (MQ=255) | GAACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTCGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGCTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTTGC > NC_002947/5304486‑5304780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |