Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,309,996	G→A	63.6%	noncoding (2564/2902 nt)	PP_23SG ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,309,996	0	G	A	63.6%	‑5.5 / 17.0	22	noncoding (2564/2902 nt)	PP_23SG	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (3/5);  new base A (8/6);  total (11/11)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTC  >  NC_002947/5309853‑5310090                                                                                                                                                |                                                                                               cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                          >  2:3702579/7‑150 (MQ=0) cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                          >  2:1877421/7‑150 (MQ=0) cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                          >  2:1877437/7‑150 (MQ=0) ccagcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAcc                                                                                          <  2:4045106/144‑1 (MQ=0)  ctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAccg                                                                                         >  2:5132898/6‑149 (MQ=0)   agcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAccgc                                                                                        >  1:3316715/5‑148 (MQ=0)             ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTaaa                                                                                  <  1:1481643/139‑1 (MQ=0)             ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                              <  2:2872224/143‑1 (MQ=0)              tggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCg                                                                             <  1:2212435/144‑1 (MQ=0)                                   agcggTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGAcc                                                        <  1:1723895/147‑1 (MQ=0)                                    gagatacGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGAcct                                                       >  2:4538450/7‑149 (MQ=0)                                    gagatacGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGAcct                                                       <  2:4920042/144‑2 (MQ=0)                                    gagatacGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGAcct                                                       <  2:4920043/144‑2 (MQ=0)                                            tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                               >  2:3961951/7‑150 (MQ=0)                                            tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                               >  1:4541859/7‑150 (MQ=0)                                            tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                               >  1:4076676/7‑150 (MQ=0)                                            tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                               <  2:896123/144‑1 (MQ=0)                                                                         gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAg                    >  1:4993863/4‑148 (MQ=1)                                                                         gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAg                    <  2:4993863/145‑1 (MQ=1)                                                                         gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                  <  2:2289507/147‑1 (MQ=1)                                                                         gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                  <  2:4635096/147‑1 (MQ=1)                                                                                         aatcGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTc  >  2:242959/4‑150 (MQ=1)                                                                                                                                                |                                                                                               CCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTC  >  NC_002947/5309853‑5310090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑