Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,310,134 C→G 52.3% noncoding (2426/2902 nt) PP_23SG ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,310,1340CG52.3% ‑6.1 / 26.4 21noncoding (2426/2902 nt)PP_23SG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base G (2/9);  total (6/15)
Fisher's exact test for biased strand distribution p-value = 3.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGC  >  NC_002947/5310008‑5310277
                                                                                                                              |                                                                                                                                               
aattGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGcc                                                                                                                          >  1:5344877/4‑150 (MQ=1)
                           cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                                                                                               >  1:3877972/4‑150 (MQ=1)
                           cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                                                                                               >  2:1935754/4‑150 (MQ=1)
                            cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                                                                                              <  2:4718262/144‑1 (MQ=1)
                            cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                                                                                              <  2:4717589/144‑1 (MQ=1)
                            cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                                                                                              <  2:4502513/144‑1 (MQ=1)
                               ctccAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACACCCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGAc                                                                                           <  2:3366843/147‑1 (MQ=1)
                                             tgcgatgaGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGtctct                                                                             >  1:3546445/4‑146 (MQ=1)
                                                          caccGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGttt                                                                <  2:925872/147‑1 (MQ=1)
                                                                                    atgtggaCTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGcc                                      <  1:4416009/144‑1 (MQ=1)
                                                                                     tatgggctctTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGcca                                     <  2:4217627/144‑2 (MQ=0)
                                                                                      gtggaCTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCtt                                    <  1:3096634/146‑1 (MQ=1)
                                                                                       tggaCTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCttt                                   <  2:2159042/147‑1 (MQ=1)
                                                                                       tggaCTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCttt                                   <  2:2159138/147‑1 (MQ=1)
                                                                                                          aggcTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAGAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTc                <  2:953395/147‑1 (MQ=1)
                                                                                                                        ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAAccatt        <  2:3836799/141‑4 (MQ=1)
                                                                                                                        ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAAccatt        >  1:3836799/4‑141 (MQ=1)
                                                                                                                        ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAACCATTCTGAgg  >  2:441582/4‑149 (MQ=0)
                                                                                                                        ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAACCATTCTGAgg  <  1:1238194/147‑2 (MQ=0)
                                                                                                                        ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGCCCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc  <  1:2522104/147‑1 (MQ=0)
                                                                                                                        ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc  <  1:2808383/147‑1 (MQ=0)
                                                                                                                              |                                                                                                                                               
AAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGC  >  NC_002947/5310008‑5310277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑