Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,310,245	A→G	54.5%	noncoding (2315/2902 nt)	PP_23SG ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,310,245	0	A	G	54.5%	‑6.2 / 9.6	11	noncoding (2315/2902 nt)	PP_23SG	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base G (3/3);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

AGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCATACACT  >  NC_002947/5310114‑5310344                                                                                                                                    |                                                                                                    aggcTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAGAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTc                                                                                   <  2:953395/147‑1 (MQ=1)               ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAAccatt                                                                           >  1:3836799/4‑141 (MQ=1)               ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAAccatt                                                                           <  2:3836799/141‑4 (MQ=1)               ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAACCATTCTGAgg                                                                     <  1:1238194/147‑2 (MQ=0)               ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAACCATTCTGAgg                                                                     >  2:441582/4‑149 (MQ=0)               ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGCCCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc                                                                     <  1:2522104/147‑1 (MQ=0)               ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc                                                                     <  1:2808383/147‑1 (MQ=0)                                                                           ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc         >  2:981829/4‑150 (MQ=0)                                                                           ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc         >  2:981859/4‑150 (MQ=0)                                                                           ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCGGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc         <  2:1453266/147‑1 (MQ=0)                                                                                  tcgtctcTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCATacacg  >  1:497697/7‑149 (MQ=0)                                                                                                                                    |                                                                                                    AGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCATACACT  >  NC_002947/5310114‑5310344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑