Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,310,265	G→A	40.0%	noncoding (2295/2902 nt)	PP_23SG ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,310,265	0	G	A	40.0%	‑6.5 / 6.3	10	noncoding (2295/2902 nt)	PP_23SG	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (2/2);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

GGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCATACACT  >  NC_002947/5310128‑5310344                                                                                                                                          |                                                                                ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAAccatt                                                                           >  1:3836799/4‑141 (MQ=1) ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAAccatt                                                                           <  2:3836799/141‑4 (MQ=1) ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAACCATTCTGAgg                                                                     <  1:1238194/147‑2 (MQ=0) ggggTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCCTTTACACTCTACGAATGATTTCCAACCATTCTGAgg                                                                     >  2:441582/4‑149 (MQ=0) ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGCCCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc                                                                     <  1:2522104/147‑1 (MQ=0) ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc                                                                     <  1:2808383/147‑1 (MQ=0)                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc         >  2:981829/4‑150 (MQ=0)                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc         >  2:981859/4‑150 (MQ=0)                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCGGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc         <  2:1453266/147‑1 (MQ=0)                                                                    tcgtctcTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCATacacg  >  1:497697/7‑149 (MQ=0)                                                                                                                                          |                                                                                GGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCATACACT  >  NC_002947/5310128‑5310344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑