Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,311,285	T→C	73.3%	noncoding (1275/2902 nt)	PP_23SG ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,311,285	0	T	C	73.3%	‑5.8 / 5.1	15	noncoding (1275/2902 nt)	PP_23SG	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (4/7);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCATCGCAATAACTGGAAGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAGGGACCGACTAACCCTGCGTCGATTAACGTTGCGCAGGAAACCTTGGTCTTTCGGCGTGCGAGTTTTTCACTCGCATTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT‑CACCTTCACAGGCTTACAGAA  >  NC_002947/5311146‑5311372                                                                                                                                            |                                                                                         ctacATCGCAATAACTGGAAGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAGGGACCGACTAACCCTGCGTCGATTAACGTTGCGCAGGAAACCTTGGTCTTTCGGCGTGCGAGTTTTTCACTCGCAt                                                                                >  1:3307797/4‑150 (MQ=1)                       ccacgAATATTAACGTGGTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGCCGGCTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCAtt                                                          <  2:5149528/146‑1 (MQ=0)                                 tacccTGTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAGGGACCGACTAACCCTGCGTCGATTAACGTTGCGCAGGAAACCTTGGTCTTTCGGCGTGCGAGTTTTTCACTCGCATTGTCGTTACTCATGTCAGCATTCGCACTTCTg                                                <  1:362390/147‑1 (MQ=1)                                     gtggttcccATCGACTACGCCTTTCGGCCTCGCCTTAGGGGCCGGCTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATAc                                            >  1:1006702/8‑150 (MQ=0)                                      tgatttCCATCGACTACGCATTTCAGCCTCGCCTTAGGGACCGACTAACCCTGCGTCGATTAACGTTGCGCAGGAAACCTTGGACTTTCGGCGAGCGAGATTTTCACTCGCATTGTCGTTACTCATGTCAGCATTCGCACTTCTGATAcc                                           <  2:1828729/147‑1 (MQ=1)                                                     actcTTTTCAGCCTCGCCTTAGTGACCGACTAACCCTGCGTCGATTAACGTTGCGCAGGAAAACTTGGTCTTTCGGCGTGCGAGTTTTTCACTCTCATTGTCGTTACTCATGTCATCATTCGCACTTCTGATACCTCCAGCAAGCTtcta                            >  1:2727155/4‑149 (MQ=1)                                                                           gggccGGCTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTAc      >  1:2497464/4‑150 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  <  1:1808910/147‑1 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  <  1:3184609/147‑1 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  <  1:4363539/147‑1 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  <  2:1103263/147‑1 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  >  2:2039655/4‑150 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  >  2:2040031/4‑150 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  <  2:2690525/147‑1 (MQ=0)                                                                               cggcTAACCCTGCTCAGATTAACTTTAAGCAGGAACCCTTGGACTTTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGaa  <  2:4662802/147‑1 (MQ=0)                                                                                                                                            |                                                                                         CTCCATCGCAATAACTGGAAGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAGGGACCGACTAACCCTGCGTCGATTAACGTTGCGCAGGAAACCTTGGTCTTTCGGCGTGCGAGTTTTTCACTCGCATTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT‑CACCTTCACAGGCTTACAGAA  >  NC_002947/5311146‑5311372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑