BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,169	T→G	8.7%	K456Q (AAG→CAG)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,169	0	T	G	8.7%	675.3 / 43.1	219	K456Q (AAG→CAG)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base T (106/94); new base G (9/10); total (115/104)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 221 total aligned reads displayed.

GGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGC  >  NC_002947/5378020‑5378318

 gAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTg                                                                                                                                                      <  2:2862283/150‑1 (MQ=255)

    ttGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGccc                                                                                                                                                   >  1:636455/1‑150 (MQ=255)

    ttGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGccc                                                                                                                                                   <  2:2896740/150‑1 (MQ=255)

     tGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCa                                                                                                                                                  <  2:1756644/150‑1 (MQ=255)

       gCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGc                                                                                                                                                >  2:960691/1‑150 (MQ=255)

       gCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGc                                                                                                                                                <  2:2430556/150‑1 (MQ=255)

       gCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGc                                                                                                                                                <  2:2773813/150‑1 (MQ=255)

        ccTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCg                                                                                                                                               >  2:1022832/1‑150 (MQ=255)

        ccTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCg                                                                                                                                               >  2:4984775/1‑150 (MQ=255)

         cTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGa                                                                                                                                              >  1:2329558/1‑150 (MQ=255)

         cTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGa                                                                                                                                              >  2:3090941/1‑150 (MQ=255)

         cTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGa                                                                                                                                              >  1:1385888/1‑150 (MQ=255)

           tGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACt                                                                                                                                            <  2:127694/150‑1 (MQ=255)

           tGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACt                                                                                                                                            >  1:2134212/1‑150 (MQ=255)

             aTCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTg                                                                                                                                          <  2:1519438/150‑1 (MQ=255)

             aTCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTg                                                                                                                                          <  2:623970/150‑1 (MQ=255)

               cACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGtt                                                                                                                                        >  2:3947745/1‑150 (MQ=255)

               cACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGtt                                                                                                                                        >  1:955358/1‑150 (MQ=255)

                 cGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCt                                                                                                                                      <  1:4639916/150‑1 (MQ=255)

                  gATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTg                                                                                                                                     >  1:5448859/1‑150 (MQ=255)

                    tCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTgcg                                                                                                                                   >  1:195408/1‑150 (MQ=255)

                    tCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTgcg                                                                                                                                   <  1:4081090/150‑1 (MQ=255)

                       aCTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAg                                                                                                                                >  1:4238539/1‑150 (MQ=255)

                        gtgttccTTGCCTGTGCCCTTGTCCTTTGCGGACACGTTCACGATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGc                                                                                                                               >  1:4976651/7‑150 (MQ=255)

                         tgttccTTGCCTGTGCCCTTGTCCTTTGCGGACACGTTCACGATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGcc                                                                                                                              >  1:904020/6‑150 (MQ=255)

                         tGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGcc                                                                                                                              >  2:3675436/1‑150 (MQ=255)

                         tGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGcc                                                                                                                              >  2:1326279/1‑150 (MQ=255)

                             tCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCt                                                                                                                          >  2:4078807/1‑150 (MQ=255)

                               ttGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTa                                                                                                                        >  1:1085434/1‑150 (MQ=255)

                               ttGCCGGTGGCCTTGCCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTa                                                                                                                        >  2:4462899/1‑150 (MQ=255)

                                tGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTAc                                                                                                                       <  1:4349243/150‑1 (MQ=255)

                                        gCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCt                                                                                                               <  1:383848/150‑1 (MQ=255)

                                          cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTg                                                                                                                                                      >  2:3825701/1‑109 (MQ=255)

                                          cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                                                             >  1:100934/1‑150 (MQ=255)

                                           ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                                                            >  1:98178/1‑150 (MQ=255)

                                           ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                                                            >  2:3584693/1‑150 (MQ=255)

                                              tCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGTTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgcagc                                                                                                         >  1:1062924/1‑150 (MQ=255)

                                                tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                       >  2:3444749/1‑150 (MQ=255)

                                                tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                       >  1:1666580/1‑150 (MQ=255)

                                                tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                       >  1:43030/1‑150 (MQ=255)

                                                tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                       >  1:2843011/1‑150 (MQ=255)

                                                         tACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGt                                                                                              <  2:2719184/150‑1 (MQ=255)

                                                           cGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCa                                                                                            <  1:1836426/150‑1 (MQ=255)

                                                            gTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCAc                                                                                           >  1:3796675/1‑150 (MQ=255)

                                                             tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACg                                                                                          <  2:1881961/150‑1 (MQ=255)

                                                             tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACg                                                                                          >  1:4854576/1‑150 (MQ=255)

                                                              gCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACgg                                                                                         <  2:4140806/150‑1 (MQ=255)

                                                              gCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACgg                                                                                         >  1:676058/1‑150 (MQ=255)

                                                                  gATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGct                                                                                     >  1:3248575/1‑150 (MQ=255)

                                                                  gATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGct                                                                                     >  1:3928419/1‑150 (MQ=255)

                                                                  gATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGct                                                                                     >  2:2199384/1‑150 (MQ=255)

                                                                  gATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccga                                                                                     <  2:2476445/150‑4 (MQ=255)

                                                                     gCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTgc                                                                                                                                    >  2:5303098/1‑100 (MQ=255)

                                                                      ccGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTgg                                                                                 <  1:3742465/150‑1 (MQ=255)

                                                                      ccGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTgg                                                                                 >  1:5200124/1‑150 (MQ=255)

                                                                        gTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                                                               <  2:5406617/150‑1 (MQ=255)

                                                                        gTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                                                               >  1:5332562/1‑150 (MQ=255)

                                                                           ggCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtc                                                                            <  2:399109/150‑1 (MQ=255)

                                                                            gCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcg                                                                           >  1:4151997/1‑150 (MQ=255)

                                                                               tCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTgtcttca                                                                        <  2:1653132/150‑2 (MQ=255)

                                                                                cGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTgtcttcag                                                                       <  1:3049076/150‑3 (MQ=255)

                                                                                     tCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                  <  1:3534671/150‑1 (MQ=255)

                                                                                      cGAAGGTCACTTCGATGTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCg                                                                 <  2:4352931/150‑1 (MQ=255)

                                                                                       gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTc                                                                                                                                       <  1:1052796/79‑1 (MQ=255)

                                                                                       gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTc                                                                                                                                       >  2:1052796/1‑79 (MQ=255)

                                                                                       gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgca                                                                                                           <  1:4874392/107‑1 (MQ=255)

                                                                                       gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgca                                                                                                           >  2:4874392/1‑107 (MQ=255)

                                                                                       gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                >  2:1192355/1‑150 (MQ=255)

                                                                                       gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                >  2:4431275/1‑150 (MQ=255)

                                                                                            tCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAaca                                                           >  1:5370355/1‑150 (MQ=255)

                                                                                            tCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAaca                                                           >  1:4489759/1‑150 (MQ=255)

                                                                                             cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                                                          >  1:4694574/1‑150 (MQ=255)

                                                                                               cTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCt                                                        <  2:5190282/150‑1 (MQ=255)

                                                                                                ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                       >  1:267210/1‑150 (MQ=255)

                                                                                                ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                       >  1:3357684/1‑150 (MQ=255)

                                                                                                ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGACCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACATTGCAGCACGTGAATGGTCACGGGGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                       >  2:3672717/1‑150 (MQ=255)

                                                                                                   gATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGa                                                    <  2:5249666/150‑1 (MQ=255)

                                                                                                         cGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtctt                                              <  1:2019787/150‑1 (MQ=255)

                                                                                                          ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                             >  1:398183/1‑150 (MQ=255)

                                                                                                          ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                             <  2:2245278/150‑1 (MQ=255)

                                                                                                             aCGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTc                                          >  1:2201014/1‑150 (MQ=255)

                                                                                                                 cacgcgGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGa                                      <  2:3916441/148‑1 (MQ=255)

                                                                                                                  gcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAt                                     <  2:4053634/150‑1 (MQ=255)

                                                                                                                        tGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATcgtcgt                               <  2:2349283/150‑1 (MQ=255)

                                                                                                                         gcgggCGGACTGTCAGCCAGGTCGACCGTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCGTGCAGCACGTGACTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                              <  2:4767440/147‑1 (MQ=255)

                                                                                                                         gCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                              <  1:130684/150‑1 (MQ=255)

                                                                                                                           tGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtgg                                   >  2:2092826/1‑143 (MQ=255)

                                                                                                                            ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                           <  1:5030180/150‑1 (MQ=255)

                                                                                                                             gCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCgg                                                                       >  1:2907571/1‑105 (MQ=255)

                                                                                                                             gCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCgg                                                                       <  2:2907571/105‑1 (MQ=255)

                                                                                                                                gAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                       <  1:4766858/150‑1 (MQ=255)

                                                                                                                                 aaTGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGttgcgttc                      <  1:2767954/150‑7 (MQ=255)

                                                                                                                                  aTGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCg                     <  2:1385888/150‑1 (MQ=255)

                                                                                                                                   tGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGa                    <  1:2401906/150‑1 (MQ=255)

                                                                                                                                        gCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgc               <  1:351911/150‑1 (MQ=255)

                                                                                                                                           aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCATCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg            <  2:3373106/150‑1 (MQ=255)

                                                                                                                                           aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg            <  2:3037164/150‑1 (MQ=255)

                                                                                                                                             gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt          <  1:1022832/150‑1 (MQ=255)

                                                                                                                                             gTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAggcgggt          <  2:5159169/150‑7 (MQ=255)

                                                                                                                                                     tGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgc  <  2:671774/150‑1 (MQ=255)

GGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGC  >  NC_002947/5378020‑5378318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`