BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,196	G→T	7.4%	Q447K (CAG→AAG) ‡	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,196	0	G	T	7.4%	639.7 / 27.8	196	Q447K (CAG→AAG) ‡	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base G (93/88); new base T (5/10); total (98/98)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

GTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCA  >  NC_002947/5378048‑5378345

 tCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCt                                                                                                                                                     >  2:4078807/1‑150 (MQ=255)

   ttGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTa                                                                                                                                                   >  1:1085434/1‑150 (MQ=255)

   ttGCCGGTGGCCTTGCCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTa                                                                                                                                                   >  2:4462899/1‑150 (MQ=255)

    tGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTAc                                                                                                                                                  <  1:4349243/150‑1 (MQ=255)

     gCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACg                                                                                                                                                 <  1:4957218/150‑1 (MQ=255)

            gCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCt                                                                                                                                          <  1:383848/150‑1 (MQ=255)

              cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                                                                                        >  1:101005/1‑150 (MQ=255)

              cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                                                                                        >  1:4275496/1‑150 (MQ=255)

              cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                                                                                        >  1:100934/1‑150 (MQ=255)

               ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                                                                                       >  2:4941927/1‑150 (MQ=255)

               ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                                                                                       <  2:2163465/150‑1 (MQ=255)

               ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                                                                                       >  1:98162/1‑150 (MQ=255)

                tGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgca                                                                                                                                      <  1:2956362/150‑1 (MQ=255)

                  tCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGTTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgcagc                                                                                                                                    >  1:1062924/1‑150 (MQ=255)

                    tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                                                  >  1:191831/1‑150 (MQ=255)

                    tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                                                  >  2:3444749/1‑150 (MQ=255)

                    tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                                                  >  1:43030/1‑150 (MQ=255)

                    tttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCAc                                                                                                                                  >  1:4869438/1‑150 (MQ=255)

                          aCCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAAt                                                                                                                            <  2:2329558/150‑1 (MQ=255)

                                 tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACg                                                                                                                     >  1:5434259/1‑150 (MQ=255)

                                 tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACg                                                                                                                     >  1:4854576/1‑150 (MQ=255)

                                 tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACg                                                                                                                     <  1:662247/150‑1 (MQ=255)

                                 tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACg                                                                                                                     <  2:1881961/150‑1 (MQ=255)

                                  gCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGACTGGTCACgg                                                                                                                    >  2:4199764/1‑150 (MQ=255)

                                   cAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGgc                                                                                                                   >  2:5290270/1‑150 (MQ=255)

                                     cgATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccg                                                                                                                 >  2:974084/2‑148 (MQ=255)

                                      gATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGct                                                                                                                >  1:3248575/1‑150 (MQ=255)

                                      gATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccga                                                                                                                <  2:2476445/150‑4 (MQ=255)

                                          ccGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTgg                                                                                                            >  1:5200124/1‑150 (MQ=255)

                                            gTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                                                                                          <  2:5406617/150‑1 (MQ=255)

                                               ggCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtc                                                                                                       <  2:399109/150‑1 (MQ=255)

                                               ggCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtc                                                                                                       <  2:3611848/150‑1 (MQ=255)

                                                gCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcg                                                                                                      >  1:4151997/1‑150 (MQ=255)

                                                   tCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGtcgtcg                                                                                                   <  2:5240399/150‑1 (MQ=255)

                                                   tCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTgtcttca                                                                                                   <  2:1653132/150‑2 (MQ=255)

                                                         tCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                                             <  1:3534671/150‑1 (MQ=255)

                                                           gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                           >  2:1192294/1‑150 (MQ=255)

                                                           gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                           >  2:4431275/1‑150 (MQ=255)

                                                                tCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAaca                                                                                      >  1:4489759/1‑150 (MQ=255)

                                                                tCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAaca                                                                                      >  2:1312848/1‑150 (MQ=255)

                                                                 cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                                                                                     >  1:4694574/1‑150 (MQ=255)

                                                                  aCTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACAcc                                                                                    >  1:687812/1‑150 (MQ=255)

                                                                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCTGGTCGAACTTGCCCAGCGAATAGTTCTCCGCCGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                  >  2:2763355/1‑150 (MQ=255)

                                                                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                  >  1:267210/1‑150 (MQ=255)

                                                                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                  >  1:3357684/1‑150 (MQ=255)

                                                                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                  >  1:3686955/1‑150 (MQ=255)

                                                                     tCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGc                                                                                 <  1:1031645/150‑1 (MQ=255)

                                                                       gATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGa                                                                               <  2:3031660/150‑1 (MQ=255)

                                                                         tttGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACGTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCGCGGGTGGTCGTCGGCAGTCGAGAACACCTGCGAct                                                                             >  2:4335744/1‑150 (MQ=255)

                                                                           tGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtc                                                                           <  2:514602/150‑1 (MQ=255)

                                                                             cGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtctt                                                                         <  1:2019787/150‑1 (MQ=255)

                                                                              ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                                                        >  1:398183/1‑150 (MQ=255)

                                                                                   gccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCgg                                                                   >  2:518669/1‑150 (MQ=255)

                                                                                     cgcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGa                                                                 >  2:1013088/1‑150 (MQ=255)

                                                                                     cacgcgGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGa                                                                 <  2:3916441/148‑1 (MQ=255)

                                                                                      gcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAt                                                                <  2:4053634/150‑1 (MQ=255)

                                                                                       cgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtg                                                               <  1:2412622/150‑1 (MQ=255)

                                                                                          ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                            <  1:3593595/150‑1 (MQ=255)

                                                                                          ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                            >  2:3763025/1‑150 (MQ=255)

                                                                                          ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                            <  2:1458800/150‑1 (MQ=255)

                                                                                           gTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtgg                                                           <  2:2890598/150‑1 (MQ=255)

                                                                                            tGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATcgtcgt                                                          <  2:2349283/150‑1 (MQ=255)

                                                                                             gcgggCGGACTGTCAGCCAGGTCGACCGTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCGTGCAGCACGTGACTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                                                         <  2:4767440/147‑1 (MQ=255)

                                                                                             gCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                                                         <  1:130684/150‑1 (MQ=255)

                                                                                               tGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgca                                                                                                                                      <  1:2905534/71‑1 (MQ=255)

                                                                                               tGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtgg                                                              >  2:2092826/1‑143 (MQ=255)

                                                                                               tGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGtt                                                       >  2:2702662/1‑150 (MQ=255)

                                                                                                ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                                                      <  1:5030180/150‑1 (MQ=255)

                                                                                                ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                                                      <  2:687812/150‑1 (MQ=255)

                                                                                                 gCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCgg                                                                                                  <  2:2907571/105‑1 (MQ=255)

                                                                                                 gCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGttgc                                                     >  1:2789669/1‑148 (MQ=255)

                                                                                                    gAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                                                  <  1:4766858/150‑1 (MQ=255)

                                                                                                     aaTGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTtctc                                                 <  1:1227248/150‑1 (MQ=255)

                                                                                                          cAGCCAGGTCGTACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGACACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCa                                            <  2:1591362/150‑1 (MQ=255)

                                                                                                           aacgaGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAg                                           >  2:5370768/5‑150 (MQ=255)

                                                                                                           aacgaGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAg                                           >  1:5295366/5‑150 (MQ=255)

                                                                                                            gCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgc                                          <  1:351911/150‑1 (MQ=255)

                                                                                                             ccAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgcg                                         <  1:3763025/150‑1 (MQ=255)

                                                                                                             ccAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgcg                                         >  1:389225/1‑150 (MQ=255)

                                                                                                               aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCATCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg                                       <  2:3373106/150‑1 (MQ=255)

                                                                                                               aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg                                       <  2:3037178/150‑1 (MQ=255)

                                                                                                                 gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                                     <  2:3935071/150‑1 (MQ=255)

                                                                                                                  tCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtc                                    <  1:2199384/150‑1 (MQ=255)

                                                                                                                    gAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcat                                  >  1:5343834/1‑150 (MQ=255)

                                                                                                                       cTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAc                               >  1:4445990/1‑150 (MQ=255)

                                                                                                                            ccAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgccgc                          <  2:1062923/150‑1 (MQ=255)

                                                                                                                             cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                         >  1:231207/1‑150 (MQ=255)

                                                                                                                              aGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAGGGTGGTGTTCTGCTCGATCAGCGCGGTCATCACGCCGccc                        >  2:4843129/1‑150 (MQ=255)

                                                                                                                               gCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                            <  2:2232947/113‑1 (MQ=255)

                                                                                                                               gCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                                                  <  2:1385741/123‑1 (MQ=255)

                                                                                                                               gCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                                                  >  1:1385741/1‑123 (MQ=255)

                                                                                                                                  aCTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATgg                    >  1:1783789/1‑150 (MQ=255)

                                                                                                                                       ttCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCg               >  2:1695714/1‑150 (MQ=255)

                                                                                                                                        tCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGa              <  1:3947745/150‑1 (MQ=255)

                                                                                                                                               aGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAg       <  2:636455/150‑1 (MQ=255)

                                                                                                                                                gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg      <  1:221667/150‑1 (MQ=255)

                                                                                                                                                gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg      <  2:1085434/150‑1 (MQ=255)

                                                                                                                                                gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg      <  1:4078634/150‑1 (MQ=255)

                                                                                                                                                   tGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTc   >  1:1689438/1‑150 (MQ=255)

                                                                                                                                                    gCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCa  >  1:4523666/1‑150 (MQ=255)

GTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCA  >  NC_002947/5378048‑5378345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`