BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,245	G→T	7.0%	D430E (GAC→GAA)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,245	0	G	T	7.0%	655.7 / 27.3	199	D430E (GAC→GAA)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base G (76/109); new base T (4/10); total (80/119)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 206 total aligned reads displayed.

GCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCC  >  NC_002947/5378096‑5378389

gCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcg                                                                                                                                                  >  1:754823/1‑150 (MQ=255)

gCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcg                                                                                                                                                  >  1:4151997/1‑150 (MQ=255)

   tCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTgtcttca                                                                                                                                               <  2:1653256/150‑2 (MQ=255)

         tCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                                                                                         <  2:609203/150‑1 (MQ=255)

         tCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                                                                                         <  1:3534648/150‑1 (MQ=255)

         tCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                                                                                         <  2:2693374/150‑1 (MQ=255)

         tCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                                                                                         >  2:4015637/1‑150 (MQ=255)

           gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                                                                       >  2:4431275/1‑150 (MQ=255)

           gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                                                                       >  2:1192355/1‑150 (MQ=255)

           gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                                                                       >  2:1031645/1‑150 (MQ=255)

                tCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAaca                                                                                                                                  >  2:2829555/1‑150 (MQ=255)

                tCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAaca                                                                                                                                  >  1:4489759/1‑150 (MQ=255)

                tCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAaca                                                                                                                                  >  1:5370355/1‑150 (MQ=255)

                 cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                                                                                                                                 >  1:4679099/1‑150 (MQ=255)

                   cTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCt                                                                                                                               <  2:5190282/150‑1 (MQ=255)

                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCTGGTCGAACTTGCCCAGCGAATAGTTCTCCGCCGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                                              >  2:2763355/1‑150 (MQ=255)

                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                                              >  1:3357684/1‑150 (MQ=255)

                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                                              >  1:3686955/1‑150 (MQ=255)

                    ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                                              >  1:4770822/1‑150 (MQ=255)

                       gATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGa                                                                                                                           <  2:5249666/150‑1 (MQ=255)

                        aTTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGAc                                                                                                                          <  2:2134212/150‑1 (MQ=255)

                         tttGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACGTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCGCGGGTGGTCGTCGGCAGTCGAGAACACCTGCGAct                                                                                                                         >  2:4335744/1‑150 (MQ=255)

                             cGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtctt                                                                                                                     <  2:1611958/150‑1 (MQ=255)

                               gCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                                                                                         <  2:11832/128‑1 (MQ=255)

                                    ccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCggg                                                                                                              <  2:976067/150‑1 (MQ=255)

                                       cgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtg                                                                                                           <  1:2412622/150‑1 (MQ=255)

                                          ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                                                                        >  2:3763025/1‑150 (MQ=255)

                                            tGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATcgtcgt                                                                                                      <  2:2349283/150‑1 (MQ=255)

                                             gcgggCGGACTGTCAGCCAGGTCGACCGTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCGTGCAGCACGTGACTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                                                                                                     <  2:4767440/147‑1 (MQ=255)

                                             gCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                                                                                                     <  1:130684/150‑1 (MQ=255)

                                                 gCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCgg                                                                                                                                              <  2:2907571/105‑1 (MQ=255)

                                                 gCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttct                                                                                                 >  1:4700344/1‑150 (MQ=255)

                                                 gCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGttgc                                                                                                 <  1:1080889/150‑3 (MQ=255)

                                                     aaTGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTtctc                                                                                             <  1:1227248/150‑1 (MQ=255)

                                                     aaTGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGttgcgttc                                                                                             <  1:2767954/150‑7 (MQ=255)

                                                      aTGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCg                                                                                            <  2:1385888/150‑1 (MQ=255)

                                                               aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCATCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg                                                                                   <  2:3373106/150‑1 (MQ=255)

                                                               aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg                                                                                   <  2:3037164/150‑1 (MQ=255)

                                                               aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAGGGTGGTGTTCTTCTCGATCAGcgcg                                                                                   >  2:13307/1‑150 (MQ=255)

                                                                 gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                                                                                 <  1:1022832/150‑1 (MQ=255)

                                                                 gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                                                                                 <  2:3935071/150‑1 (MQ=255)

                                                                 gTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAggcgggt                                                                                 <  2:5159169/150‑7 (MQ=255)

                                                                  tCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtc                                                                                <  1:2199384/150‑1 (MQ=255)

                                                                         tGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgc                                                                         <  2:671774/150‑1 (MQ=255)

                                                                            ccAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgccgc                                                                      <  2:1484441/150‑1 (MQ=255)

                                                                            ccAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgccgc                                                                      <  2:1062924/150‑1 (MQ=255)

                                                                             cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                                                                     >  1:3534776/1‑150 (MQ=255)

                                                                             cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                                                                     >  1:231207/1‑150 (MQ=255)

                                                                             cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                                                                     >  1:4006107/1‑150 (MQ=255)

                                                                                cGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCAt                                                                  >  1:3839807/1‑150 (MQ=255)

                                                                                  aCTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATgg                                                                >  1:3611950/1‑150 (MQ=255)

                                                                                  aCTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATgg                                                                >  1:1783789/1‑150 (MQ=255)

                                                                                     tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt                                                             <  2:5332562/150‑1 (MQ=255)

                                                                                        tCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGa                                                          <  1:3947745/150‑1 (MQ=255)

                                                                                          tGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa                                                        >  1:4455218/1‑150 (MQ=255)

                                                                                                gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                  <  2:4267956/150‑1 (MQ=255)

                                                                                                gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                  <  1:1326279/150‑1 (MQ=255)

                                                                                                gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                  <  1:4078634/150‑1 (MQ=255)

                                                                                                gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                  <  1:221667/150‑1 (MQ=255)

                                                                                                   tGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTc                                               >  1:1689438/1‑150 (MQ=255)

                                                                                                   tGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTc                                               >  1:2596485/1‑150 (MQ=255)

                                                                                                    gCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCa                                              >  1:4523666/1‑150 (MQ=255)

                                                                                                      ttACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATggtggt                                                                                                      <  2:2974917/92‑1 (MQ=255)

                                                                                                        aCGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGTCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCgg                                          >  2:3200358/1‑150 (MQ=255)

                                                                                                          gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                                        >  1:2071799/1‑150 (MQ=255)

                                                                                                            tCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg                                      >  1:5009008/1‑150 (MQ=255)

                                                                                                              aCCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGAc                                    <  2:4088311/150‑1 (MQ=255)

                                                                                                              aCCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGAc                                    <  1:1192294/150‑1 (MQ=255)

                                                                                                               ccTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACg                                   >  2:34370/1‑150 (MQ=255)

                                                                                                                 ttGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTc                                 <  2:4489858/150‑1 (MQ=255)

                                                                                                                 ttGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTc                                 >  2:3542578/1‑150 (MQ=255)

                                                                                                                   gcagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCca                               <  2:951746/150‑1 (MQ=255)

                                                                                                                    cagctCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGTACTCCTGCGACTTCTTTGTCGTGATGGTGGTTTTCTTCTCTATCAGCGCGTTCATCACGCCGCCCATGGGTTCTATACCCAGGGTCAGCGGGCTGACGTCcat                              <  2:5072860/150‑2 (MQ=255)

                                                                                                                     agcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagc                             >  2:4777419/1‑150 (MQ=255)

                                                                                                                     agcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagc                             >  1:5308730/1‑150 (MQ=255)

                                                                                                                       caCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcag                              >  1:3923805/1‑147 (MQ=255)

                                                                                                                       caCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcag                              <  2:3923805/147‑1 (MQ=255)

                                                                                                                        aCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagc                          >  1:1574073/1‑150 (MQ=255)

                                                                                                                         cGTGACTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagca                         <  1:5290270/150‑1 (MQ=255)

                                                                                                                         cGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagca                         <  1:3031742/150‑1 (MQ=255)

                                                                                                                         cGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagca                         <  2:2240812/150‑1 (MQ=255)

                                                                                                                          gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag                        <  1:4431275/150‑1 (MQ=255)

                                                                                                                          gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag                        <  1:3675398/150‑1 (MQ=255)

                                                                                                                          gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag                        <  2:3326904/150‑1 (MQ=255)

                                                                                                                           tGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAgg                       >  2:5437528/1‑150 (MQ=255)

                                                                                                                           tGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAgg                       <  2:1783789/150‑1 (MQ=255)

                                                                                                                             aaTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGAc                     <  2:3248575/150‑1 (MQ=255)

                                                                                                                                   cACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCttt               <  1:581195/150‑1 (MQ=255)

                                                                                                                                    aCGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTc              >  1:5163268/1‑150 (MQ=255)

                                                                                                                                      ggccgactGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGAc            <  1:4511834/144‑1 (MQ=255)

                                                                                                                                      ggCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCAc            <  2:3839807/150‑1 (MQ=255)

                                                                                                                                      ggCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCAc            <  2:100934/150‑1 (MQ=255)

                                                                                                                                      ggCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCAc            <  2:4867909/150‑1 (MQ=255)

                                                                                                                                           tctGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCAc       >  1:5205923/1‑150 (MQ=255)

                                                                                                                                             tgattGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTCGccc     >  1:4900714/4‑149 (MQ=255)

                                                                                                                                               gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc   <  1:3090941/150‑1 (MQ=255)

                                                                                                                                               gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc   <  2:4679099/150‑1 (MQ=255)

                                                                                                                                               gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc   <  1:2829555/150‑1 (MQ=255)

                                                                                                                                               gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc   >  1:3606300/1‑150 (MQ=255)

                                                                                                                                                ttGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTCGccctgc  <  2:1999637/150‑5 (MQ=255)

GCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCC  >  NC_002947/5378096‑5378389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`