BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,262	C→T	7.5%	V425M (GTG→ATG) ‡	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,262	0	C	T	7.5%	558.6 / 24.5	186	V425M (GTG→ATG) ‡	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base C (63/109); new base T (4/10); total (67/119)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 196 total aligned reads displayed.

CACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCA  >  NC_002947/5378113‑5378410

cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                                                                                                                                                      >  1:4694574/1‑150 (MQ=255)

  cTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCt                                                                                                                                                    <  2:5190282/150‑1 (MQ=255)

   ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                                                                   >  1:3686955/1‑150 (MQ=255)

   ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                                                                   >  1:267210/1‑150 (MQ=255)

    tCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGc                                                                                                                                                  <  1:1031645/150‑1 (MQ=255)

       aTTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGAc                                                                                                                                               <  2:2134212/150‑1 (MQ=255)

        tttGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACGTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCGCGGGTGGTCGTCGGCAGTCGAGAACACCTGCGAct                                                                                                                                              >  2:4335744/1‑150 (MQ=255)

            cGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtctt                                                                                                                                          <  2:1611958/150‑1 (MQ=255)

             ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                                                                                                                         >  1:398183/1‑150 (MQ=255)

             ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                                                                                                                         <  2:2245278/150‑1 (MQ=255)

                    cacgcgGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGa                                                                                                                                  <  2:3916441/148‑1 (MQ=255)

                     gcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAt                                                                                                                                 <  2:4053634/150‑1 (MQ=255)

                      cgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtg                                                                                                                                <  1:2412622/150‑1 (MQ=255)

                         ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                                                                                             >  2:3763025/1‑150 (MQ=255)

                          gTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtgg                                                                                                                            <  2:2890598/150‑1 (MQ=255)

                           tGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATcgtcgt                                                                                                                           <  2:2349283/150‑1 (MQ=255)

                            gcgggCGGACTGTCAGCCAGGTCGACCGTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCGTGCAGCACGTGACTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                                                                                                                          <  2:4767440/147‑1 (MQ=255)

                              tGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGtt                                                                                                                        >  2:2702662/1‑150 (MQ=255)

                               ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                                                                                                                       <  2:687812/150‑1 (MQ=255)

                               ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                                                                                                                       <  1:5030180/150‑1 (MQ=255)

                                gCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGttgc                                                                                                                      >  1:2789669/1‑148 (MQ=255)

                                   gAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                                                                                                                   <  1:4766858/150‑1 (MQ=255)

                                     aTGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCg                                                                                                                 <  2:1385888/150‑1 (MQ=255)

                                          aacgaGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAg                                                                                                            >  1:5295366/5‑150 (MQ=255)

                                          aacgaGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAg                                                                                                            >  2:5370768/5‑150 (MQ=255)

                                            ccAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgcg                                                                                                          <  1:3763025/150‑1 (MQ=255)

                                            ccAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgcg                                                                                                          >  1:389225/1‑150 (MQ=255)

                                              aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCATCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg                                                                                                        <  2:3373106/150‑1 (MQ=255)

                                              aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGcgcg                                                                                                        <  2:3037164/150‑1 (MQ=255)

                                              aGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAGGGTGGTGTTCTTCTCGATCAGcgcg                                                                                                        >  2:13307/1‑150 (MQ=255)

                                                gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                                                                                                      <  2:3935071/150‑1 (MQ=255)

                                                gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                                                                                                      <  1:1022832/150‑1 (MQ=255)

                                                gTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAggcgggt                                                                                                      <  2:5159169/150‑7 (MQ=255)

                                                 tCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtc                                                                                                     <  1:2199384/150‑1 (MQ=255)

                                                  cGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtca                                                                                                    <  2:383067/150‑1 (MQ=255)

                                                   gAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcat                                                                                                   >  1:5343834/1‑150 (MQ=255)

                                                           ccAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgccgc                                                                                           <  2:1484441/150‑1 (MQ=255)

                                                           ccAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgccgc                                                                                           <  2:1062923/150‑1 (MQ=255)

                                                            cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                                                                                          >  1:3534776/1‑150 (MQ=255)

                                                            cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                                                                                          >  1:231207/1‑150 (MQ=255)

                                                              gCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                                                                                             <  2:2232947/113‑1 (MQ=255)

                                                              gCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                                                                                                                   >  1:1385741/1‑123 (MQ=255)

                                                                 aCTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATgg                                                                                     >  1:3611950/1‑150 (MQ=255)

                                                                 aCTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATgg                                                                                     >  1:1783789/1‑150 (MQ=255)

                                                                        cTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGAt                                                                              <  2:955358/150‑1 (MQ=255)

                                                                         tGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa                                                                             >  1:4455218/1‑150 (MQ=255)

                                                                          gcgcAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATAc                                                                            >  1:5200629/1‑150 (MQ=255)

                                                                          gcgcAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATAc                                                                            >  1:1235170/1‑150 (MQ=255)

                                                                              aGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAg                                                                        <  2:636455/150‑1 (MQ=255)

                                                                               gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                                       <  2:1085434/150‑1 (MQ=255)

                                                                               gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                                       <  1:4078807/150‑1 (MQ=255)

                                                                               gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                                       <  1:221667/150‑1 (MQ=255)

                                                                                     ttACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCATTCGAGAACACCTGCGACTTCTTGGTCGGGATggtggt                                                                                                                           >  1:2974917/1‑92 (MQ=255)

                                                                                     ttACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATggtggt                                                                                                                           <  2:2974917/92‑1 (MQ=255)

                                                                                     ttACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGc                                                                 >  1:1622844/1‑150 (MQ=255)

                                                                                       aCGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGTCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCgg                                                               >  2:3200358/1‑150 (MQ=255)

                                                                                         gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                                                             >  1:2071799/1‑150 (MQ=255)

                                                                                         gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                                                             <  2:442630/150‑1 (MQ=255)

                                                                                           tCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg                                                           >  1:4028482/1‑150 (MQ=255)

                                                                                           tCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg                                                           <  2:4238539/150‑1 (MQ=255)

                                                                                             aCCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGAc                                                         <  1:960691/150‑1 (MQ=255)

                                                                                             aCCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGAc                                                         <  1:1192355/150‑1 (MQ=255)

                                                                                              ccTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACg                                                        >  2:34370/1‑150 (MQ=255)

                                                                                                ttGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTc                                                      <  2:4489858/150‑1 (MQ=255)

                                                                                                ttGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTc                                                      >  1:5269734/1‑150 (MQ=255)

                                                                                                ttGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTc                                                      >  2:3542578/1‑150 (MQ=255)

                                                                                                  gcagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGACAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCca                                                    <  2:2767857/150‑1 (MQ=255)

                                                                                                   cagctCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGTACTCCTGCGACTTCTTTGTCGTGATGGTGGTTTTCTTCTCTATCAGCGCGTTCATCACGCCGCCCATGGGTTCTATACCCAGGGTCAGCGGGCTGACGTCcat                                                   <  2:5072860/150‑2 (MQ=255)

                                                                                                   cagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcag                                                   <  2:4869438/150‑1 (MQ=255)

                                                                                                    agcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagc                                                  >  2:4777419/1‑150 (MQ=255)

                                                                                                      caCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcag                                                   <  2:3923805/147‑1 (MQ=255)

                                                                                                         gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag                                             <  2:98162/150‑1 (MQ=255)

                                                                                                         gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag                                             <  1:3675398/150‑1 (MQ=255)

                                                                                                         gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag                                             <  2:3326904/150‑1 (MQ=255)

                                                                                                         gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag                                             <  1:4431275/150‑1 (MQ=255)

                                                                                                          tGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAgg                                            >  2:5437528/1‑150 (MQ=255)

                                                                                                          tGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAgg                                            <  2:1783789/150‑1 (MQ=255)

                                                                                                            aaTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGAc                                          <  2:3248575/150‑1 (MQ=255)

                                                                                                              gggTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGAGACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGt                                        <  1:4941927/149‑1 (MQ=255)

                                                                                                               cgTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTc                                       <  1:4848448/149‑1 (MQ=255)

                                                                                                                  cACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCttt                                    <  1:581195/150‑1 (MQ=255)

                                                                                                                  cACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCttt                                    <  1:4462899/150‑1 (MQ=255)

                                                                                                                   aCGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTc                                   >  1:4561315/1‑150 (MQ=255)

                                                                                                                    cGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCa                                  <  1:3924795/150‑1 (MQ=255)

                                                                                                                    cGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCa                                  <  1:1312848/150‑1 (MQ=255)

                                                                                                                     ggccgactGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGAc                                 <  1:4512200/144‑1 (MQ=255)

                                                                                                                     ggCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCAc                                 <  2:101005/150‑1 (MQ=255)

                                                                                                                              gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc                        <  2:4679099/150‑1 (MQ=255)

                                                                                                                              gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc                        <  2:5269734/150‑1 (MQ=255)

                                                                                                                              gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc                        <  1:3090941/150‑1 (MQ=255)

                                                                                                                              gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc                        >  1:3606300/1‑150 (MQ=255)

                                                                                                                               ttGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTCGccctgc                       <  2:1999637/150‑5 (MQ=255)

                                                                                                                                         cAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCAc             <  1:1148667/150‑1 (MQ=255)

                                                                                                                                              gagaACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCt                                      >  2:257870/1‑120 (MQ=255)

                                                                                                                                              gagaACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCt                                      <  1:257870/120‑1 (MQ=255)

                                                                                                                                               agcacacCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGa       <  1:3444749/147‑1 (MQ=255)

                                                                                                                                                gaACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGAt      <  2:4151997/150‑1 (MQ=255)

                                                                                                                                                  acacCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATgg    <  2:4854576/150‑1 (MQ=255)

                                                                                                                                                  acacCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATgg    <  1:1695714/150‑1 (MQ=255)

                                                                                                                                                    acCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGgca  <  2:4275496/150‑1 (MQ=255)

CACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCA  >  NC_002947/5378113‑5378410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`