BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,764	C→A	10.5%	L257L (CTG→CTT)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,764	0	C	A	10.5%	557.1 / 40.8	180	L257L (CTG→CTT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base C (74/87); new base A (7/12); total (81/99)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

GACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCG  >  NC_002947/5378615‑5378911

gACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGcc                                                                                                                                                     <  2:4845151/150‑1 (MQ=255)

   tCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCaga                                                                                                                                                  >  2:5046221/1‑150 (MQ=255)

        cTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATc                                                                                                                                             <  2:3836893/150‑1 (MQ=255)

         ttGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCg                                                                                                                                            <  1:2870164/150‑1 (MQ=255)

              gcgGGAGATCTTCACGTTCAGGAGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGttctt                                                                                                                                       <  2:1192160/150‑1 (MQ=255)

               cgGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGTCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGttcttc                                                                                                                                      <  2:828992/150‑1 (MQ=255)

                 ggAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAg                                                                                                                                    <  2:1549872/150‑1 (MQ=255)

                         tCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGc                                                                                                                            >  2:974030/1‑150 (MQ=255)

                          cACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGcc                                                                                                                           >  1:2415241/1‑150 (MQ=255)

                          cACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGcc                                                                                                                           <  1:5087976/150‑1 (MQ=255)

                           aCGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGccc                                                                                                                          <  1:4921194/150‑1 (MQ=255)

                            ttgcaaGGTGCTTAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGccct                                                                                                                         <  1:2301631/145‑2 (MQ=255)

                             gTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGa                                                                                                                        >  2:1863722/1‑150 (MQ=255)

                              ttCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGAc                                                                                                                       <  1:1491203/150‑1 (MQ=255)

                               caaGGTGCTTAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGccctgtt                                                                                                                      >  1:5190020/3‑146 (MQ=255)

                                 aGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGActct                                                                                                                    >  2:5302135/1‑150 (MQ=255)

                                  ggTGCTTAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGccctgttcct                                                                                                                   >  1:2314414/1‑143 (MQ=255)

                                    tGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttc                                                                                                                 <  1:355636/150‑1 (MQ=255)

                                     gCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCTGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttct                                                                                                                >  1:645803/1‑150 (MQ=255)

                                     gCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttct                                                                                                                <  1:4838819/150‑1 (MQ=255)

                                     gCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttct                                                                                                                <  2:603001/150‑1 (MQ=255)

                                     gCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttct                                                                                                                <  2:603045/150‑1 (MQ=255)

                                      cTTAGGACCGGTGGCATCTGCAGTGATGTACGGCTGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                               <  1:369373/150‑1 (MQ=255)

                                      cTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                               <  1:1656585/150‑1 (MQ=255)

                                      cTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACtctttctt                                                                                                               <  2:3462834/150‑1 (MQ=255)

                                       ttAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTg                                                                                                              >  1:603879/1‑150 (MQ=255)

                                       ttAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTg                                                                                                              >  1:2892962/1‑150 (MQ=255)

                                       ttAGGACCGGTCTGGTCAGCCGTGATGAATGGCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTg                                                                                                              >  2:3302527/1‑150 (MQ=255)

                                        tAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGa                                                                                                             <  2:4906413/150‑1 (MQ=255)

                                          ggACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTGTCTTGAAc                                                                                                           >  2:3499741/1‑150 (MQ=255)

                                           gACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTcc                                                                                                                                <  1:4300294/128‑1 (MQ=255)

                                           gACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACt                                                                                                          >  1:2768720/1‑150 (MQ=255)

                                               ggTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgtc                                                                                                      <  2:3257870/150‑1 (MQ=255)

                                               ggTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgtc                                                                                                      <  1:1772482/150‑1 (MQ=255)

                                               ggTGGCATCTGCAGTGATGTACGGCAGGTTGACGACGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGCACtcgtc                                                                                                      <  2:5405738/150‑1 (MQ=255)

                                                 tGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGa                                                                                                    <  1:974030/150‑1 (MQ=255)

                                                     aTCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAg                                                                                                <  2:3728169/150‑1 (MQ=255)

                                                     aTCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAg                                                                                                >  2:3852165/1‑150 (MQ=255)

                                                         gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCaga                                                                                                                                                  <  1:1047174/96‑1 (MQ=255)

                                                         gCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCaga                                                                                                                                                  >  2:1047174/1‑96 (MQ=255)

                                                          cAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAACTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtc                                                                                                         >  2:1750021/1‑136 (MQ=255)

                                                          cAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAACTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtc                                                                                                         <  1:1750021/136‑1 (MQ=255)

                                                              gATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGAt                                                                                       <  2:2778458/150‑1 (MQ=255)

                                                              gATGTACGGCAGGTTGACGTCGGTCGACTGAGAGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGAt                                                                                       >  1:4277558/1‑150 (MQ=255)

                                                               aTGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATc                                                                                      >  1:3343/1‑150 (MQ=255)

                                                                tgaatggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCa                                                                                     <  2:2413450/145‑1 (MQ=255)

                                                                 gTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAg                                                                                    >  2:4809859/1‑150 (MQ=255)

                                                                     ggCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGgcgc                                                                                >  1:748466/1‑150 (MQ=255)

                                                                     ggCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGgcgc                                                                                >  1:1583697/1‑150 (MQ=255)

                                                                      gCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCa                                                                               <  1:3913704/150‑1 (MQ=255)

                                                                        aGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATg                                                                             >  1:1266681/1‑150 (MQ=255)

                                                                         ggTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGt                                                                            <  2:4085696/150‑1 (MQ=255)

                                                                          gTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                           >  1:597710/1‑150 (MQ=255)

                                                                           ttGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCg                                                                          <  2:5128637/150‑1 (MQ=255)

                                                                            tGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGa                                                                         <  1:3278734/150‑1 (MQ=255)

                                                                             gACGTCGGTCGACTGAGCGGACGACCGCTCGATCTTGGCTTTGTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                        <  2:2955738/150‑1 (MQ=255)

                                                                             gACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                        <  2:3136956/150‑1 (MQ=255)

                                                                             gACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                        <  2:1301451/150‑1 (MQ=255)

                                                                                gtcggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTc                                                                     >  1:464344/1‑150 (MQ=255)

                                                                                  cggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCtt                                                                   >  1:5328596/1‑150 (MQ=255)

                                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                  <  2:4658246/150‑1 (MQ=255)

                                                                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                  <  1:1115724/150‑1 (MQ=255)

                                                                                    gtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCg                                                                 <  1:2186037/150‑1 (MQ=255)

                                                                                        aCTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCAc                                                             >  2:4311574/1‑150 (MQ=255)

                                                                                           gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga                                                          <  1:386671/150‑3 (MQ=255)

                                                                                           gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga                                                          <  2:1089387/150‑3 (MQ=255)

                                                                                             gCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAgg                                                        <  1:1675146/150‑1 (MQ=255)

                                                                                              cGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGa                                                       >  1:4502025/1‑150 (MQ=255)

                                                                                              cGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGacg                                                                                 <  2:1731704/124‑1 (MQ=255)

                                                                                              cGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGacg                                                                                 >  1:1731704/1‑124 (MQ=255)

                                                                                               ggACGACAGCTCGATCTTGGCTTTTTCTGCGGATTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa                                                      <  2:4477336/150‑1 (MQ=255)

                                                                                                gacgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAg                                                     <  2:4231532/150‑1 (MQ=255)

                                                                                                gacgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAg                                                     <  1:1203361/150‑1 (MQ=255)

                                                                                                 acgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAgg                                                                                               <  2:3759775/107‑1 (MQ=255)

                                                                                                 acgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAgg                                                                                               >  1:3759775/1‑107 (MQ=255)

                                                                                                 acgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAgg                                                    >  2:1390253/1‑150 (MQ=255)

                                                                                                   gacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                           >  1:1664400/1‑125 (MQ=255)

                                                                                                         tCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCGGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCg                                            >  2:4035620/1‑150 (MQ=255)

                                                                                                         tCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCg                                            >  1:2378896/1‑150 (MQ=255)

                                                                                                           gATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGtt                                          <  1:3056297/150‑1 (MQ=255)

                                                                                                            aTCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTg                                         <  1:1291798/150‑1 (MQ=255)

                                                                                                            aTCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTg                                         <  2:2349542/150‑1 (MQ=255)

                                                                                                                  gCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTa                                                                                             <  2:642117/92‑1 (MQ=255)

                                                                                                                  gCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGcc                                   >  1:4817157/1‑150 (MQ=255)

                                                                                                                      tttCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTa                               <  2:2498477/150‑1 (MQ=255)

                                                                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                           <  1:854340/150‑1 (MQ=255)

                                                                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                           <  2:3038901/150‑1 (MQ=255)

                                                                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                           >  1:1000104/1‑150 (MQ=255)

                                                                                                                             ggCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGaa                        >  1:1344179/1‑150 (MQ=255)

                                                                                                                                 tcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTgg                    <  1:2640523/150‑1 (MQ=255)

                                                                                                                                  cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                   <  1:3347300/150‑1 (MQ=255)

                                                                                                                                  cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                   >  2:1430098/1‑150 (MQ=255)

                                                                                                                                  cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                   <  1:3208567/150‑1 (MQ=255)

                                                                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                >  1:1004507/1‑115 (MQ=255)

                                                                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                <  2:1004507/115‑1 (MQ=255)

                                                                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTggcta                 >  1:3132335/1‑143 (MQ=255)

                                                                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTggcta                 <  2:3132335/146‑4 (MQ=255)

                                                                                                                                                 gggCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACtt    <  2:4277558/150‑1 (MQ=255)

                                                                                                                                                   gCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCg  >  2:687009/1‑150 (MQ=255)

                                                                                                                                                     cAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGc                                    <  1:4057684/114‑1 (MQ=255)

GACTCCAGCTTGGCGCGGGAGATCTTCACGTTCAGGTGCTTAGGACCGGTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCG  >  NC_002947/5378615‑5378911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`