BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,812	G→A	8.2%	V241V (GTC→GTT)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,812	0	G	A	8.2%	427.8 / 20.7	146	V241V (GTC→GTT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base G (62/72); new base A (3/9); total (65/81)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 151 total aligned reads displayed.

GTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAAC  >  NC_002947/5378663‑5378961

gTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACtcgtcg                                                                                                                                                       >  1:3210496/1‑150 (MQ=255)

 tGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGa                                                                                                                                                      <  1:974030/150‑1 (MQ=255)

     aTCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAg                                                                                                                                                  >  1:3084312/1‑150 (MQ=255)

     aTCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAg                                                                                                                                                  >  2:3852165/1‑150 (MQ=255)

              gATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGAt                                                                                                                                         <  2:2778458/150‑1 (MQ=255)

              gATGTACGGCAGGTTGACGTCGGTCGACTGAGAGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGAt                                                                                                                                         >  1:4277558/1‑150 (MQ=255)

               aTGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATc                                                                                                                                        >  1:3343/1‑150 (MQ=255)

                tgaatggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCa                                                                                                                                       <  1:3833449/145‑1 (MQ=255)

                tgaatggCAGGTTGATTTCGGTCTGCTGAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCa                                                                                                                                       <  2:2413450/145‑1 (MQ=255)

                 gTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAg                                                                                                                                      >  2:4809859/1‑150 (MQ=255)

                  tACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAgg                                                                                                                                     <  2:5205923/150‑1 (MQ=255)

                     ggCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGgcgc                                                                                                                                  >  1:1583697/1‑150 (MQ=255)

                     ggCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGgcgc                                                                                                                                  >  1:748466/1‑150 (MQ=255)

                      gCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCa                                                                                                                                 <  1:3913704/150‑1 (MQ=255)

                        aGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATg                                                                                                                               >  1:1266681/1‑150 (MQ=255)

                         ggTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGt                                                                                                                              <  2:4085696/150‑1 (MQ=255)

                          gTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                                                                             >  1:597710/1‑150 (MQ=255)

                          gTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                                                                             >  2:4732700/1‑150 (MQ=255)

                            tGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGa                                                                                                                           <  1:3278734/150‑1 (MQ=255)

                             gACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                                                                          <  2:1301451/150‑1 (MQ=255)

                             gACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                                                                          <  2:2955827/150‑1 (MQ=255)

                             gACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGaa                                                                                                                          >  2:2634006/1‑150 (MQ=255)

                                gtcggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTc                                                                                                                       >  1:464344/1‑150 (MQ=255)

                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                                                    <  2:4658246/150‑1 (MQ=255)

                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                                                    <  2:3913436/150‑1 (MQ=255)

                                   ggtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTc                                                                                                                    <  1:1115724/150‑1 (MQ=255)

                                    gtcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCg                                                                                                                   >  2:1773412/1‑150 (MQ=255)

                                     tcgACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGc                                                                                                                  <  1:4287330/150‑1 (MQ=255)

                                        aCTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCAc                                                                                                               >  2:4311574/1‑150 (MQ=255)

                                           gAGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGccga                                                                                                            <  1:386671/150‑3 (MQ=255)

                                            aGCGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGCCGAg                                                                                                           <  1:863700/150‑1 (MQ=255)

                                             gCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAgg                                                                                                          <  1:1675146/150‑1 (MQ=255)

                                              cGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGa                                                                                                         >  1:4502025/1‑150 (MQ=255)

                                              cGGACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGacg                                                                                                                                   >  1:1731704/1‑124 (MQ=255)

                                               ggACGACAGCTCGATCTTGGCTTTTTCTGCGGATTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa                                                                                                        <  2:4477336/150‑1 (MQ=255)

                                               ggACGAAAGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGCCGAGGaa                                                                                                        <  2:3484172/150‑1 (MQ=255)

                                                 acgacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAgg                                                                                                                                                 <  2:3759775/107‑1 (MQ=255)

                                                   gacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                                                                             >  1:1664400/1‑125 (MQ=255)

                                                   gacAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTc                                                                                                                             <  2:1664400/125‑1 (MQ=255)

                                                   gaaaGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGCCGAGGAACgtg                                                                                                    >  1:4805648/4‑150 (MQ=255)

                                                    aaaGCTCGATCTTGGCTTTTTCGGCAGCTTCCTTGAGGCGCTGCAGAGCAAGCTTGTCTTTCTTAAGATCGATGCCCTGTTCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGCATGTCAAAGTCTTCACCGCCGAGGAACgtgt                                                                                                   <  1:2664692/148‑1 (MQ=255)

                                                     cAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                                                                  <  1:2317474/150‑1 (MQ=255)

                                                         tCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCg                                                                                              >  1:2378896/1‑150 (MQ=255)

                                                         tCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCg                                                                                              >  2:108493/1‑150 (MQ=255)

                                                           gATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGtt                                                                                            <  1:3056297/150‑1 (MQ=255)

                                                            aTCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTg                                                                                           <  1:1291798/150‑1 (MQ=255)

                                                            aTCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTg                                                                                           <  2:1154880/150‑1 (MQ=255)

                                                               ttGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTa                                                                                        >  1:3194387/1‑150 (MQ=255)

                                                               ttGGCTTTTTCTGCGGCTTCTTTCACACGCTGCAGGGACAGAGGATCGTTCTTCAGGTCCATGCACGACACTATCTTGAAATAGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACACAGGAAGGTGTAGCCGTAGGTa                                                                                        >  2:4970600/1‑150 (MQ=255)

                                                                  gCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTa                                                                                                                                               >  1:642117/1‑92 (MQ=255)

                                                                  gCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTa                                                                                                                                               <  2:642117/92‑1 (MQ=255)

                                                                   cTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCa                                                                                    >  2:3833893/1‑150 (MQ=255)

                                                                    tttttCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTTGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAg                                                                                   >  2:2437317/1‑150 (MQ=255)

                                                                      tttCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTa                                                                                 <  1:5134262/150‑1 (MQ=255)

                                                                        tCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACt                                                                               >  2:504798/1‑150 (MQ=255)

                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                                                                             <  1:854340/150‑1 (MQ=255)

                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                                                                             <  2:3038901/150‑1 (MQ=255)

                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                                                                             >  2:5307234/1‑150 (MQ=255)

                                                                          tGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTc                                                                             >  1:1000104/1‑150 (MQ=255)

                                                                             ggCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGaa                                                                          >  1:1344179/1‑150 (MQ=255)

                                                                                 tcttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTgg                                                                      <  1:5273847/150‑1 (MQ=255)

                                                                                  cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                                                                     >  2:1430098/1‑150 (MQ=255)

                                                                                  cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                                                                     <  2:3194387/150‑1 (MQ=255)

                                                                                  cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                                                                     <  1:3347300/150‑1 (MQ=255)

                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                                                                  >  1:1004507/1‑115 (MQ=255)

                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTc                                                                                                  <  2:1004507/115‑1 (MQ=255)

                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTggcta                                                                   >  1:3132335/1‑143 (MQ=255)

                                                                                        gACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTggcta                                                                   <  2:3132335/146‑4 (MQ=255)

                                                                                                 gggCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACtt                                                      <  2:4277558/150‑1 (MQ=255)

                                                                                                   gCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCg                                                    >  2:687009/1‑150 (MQ=255)

                                                                                                     cAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGc                                                                                      <  1:4057684/114‑1 (MQ=255)

                                                                                                     cAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGc                                                                                      >  2:4057684/1‑114 (MQ=255)

                                                                                                          gATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGAttt                                             >  2:1660933/1‑150 (MQ=255)

                                                                                                           aTCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTc                                            <  1:5046221/150‑1 (MQ=255)

                                                                                                            tCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCg                                           <  1:782967/150‑1 (MQ=255)

                                                                                                              gTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGAt                                         <  1:1773412/150‑1 (MQ=255)

                                                                                                              gTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGAt                                         >  2:4648117/1‑150 (MQ=255)

                                                                                                              gTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGAt                                         <  1:4516283/150‑1 (MQ=255)

                                                                                                                tcttcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGa                                       >  2:2319383/1‑150 (MQ=255)

                                                                                                                  ttcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGAcc                                     >  1:118105/1‑150 (MQ=255)

                                                                                                                     aGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGaa                                  >  2:2537848/1‑150 (MQ=255)

                                                                                                                      ggTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGaag                                 <  2:118105/150‑2 (MQ=255)

                                                                                                                       gTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAAc                                >  1:3755353/1‑150 (MQ=255)

                                                                                                                                   ctctTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTacc                    <  1:5302135/150‑1 (MQ=255)

                                                                                                                                   ctctTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTacc                    <  2:1440049/150‑1 (MQ=255)

                                                                                                                                    tctttcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGACCTGGTGCTCACCGTCGACTTCGGCGTTTTCGATGACCGAAACGTCGAAGGTacca                   <  2:3343/150‑1 (MQ=255)

                                                                                                                                         cttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTaccaccacc              >  2:675970/1‑150 (MQ=255)

                                                                                                                                          ttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTg                                                                       <  2:362646/92‑1 (MQ=255)

                                                                                                                                          ttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTg                                                                       >  1:362646/1‑92 (MQ=255)

                                                                                                                                          ttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCAccc             >  1:3441647/1‑150 (MQ=255)

                                                                                                                                          ttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCAccc             >  1:3041138/1‑150 (MQ=255)

                                                                                                                                          ttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCAccc             >  2:128636/1‑150 (MQ=255)

                                                                                                                                           tGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCa            <  2:4501987/150‑1 (MQ=255)

                                                                                                                                            gAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAg           <  2:665210/150‑1 (MQ=255)

                                                                                                                                               cTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTc        <  2:2892962/150‑1 (MQ=255)

                                                                                                                                               cTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTc        <  2:603879/150‑1 (MQ=255)

                                                                                                                                                tcgtcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCa       >  2:2171277/1‑150 (MQ=255)

                                                                                                                                                tcgtcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCa       >  2:1751095/1‑150 (MQ=255)

                                                                                                                                                  gtcgACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATa     <  1:3499741/150‑1 (MQ=255)

                                                                                                                                                     gACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAAc  >  2:3115582/1‑150 (MQ=255)

GTGGCATCTGCAGTGATGTACGGCAGGTTGACGTCGGTCGACTGAGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAAC  >  NC_002947/5378663‑5378961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`