BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,390,853	T→G	5.3%	L798R (CTG→CGG)	dld2 →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,390,853	0	T	G	5.3%	577.3 / 15.6	171	L798R (CTG→CGG)	dld2	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (78/83); new base G (5/4); total (83/88)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 174 total aligned reads displayed.

CAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAGCC  >  NC_002947/5390704‑5391002

cAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCt                                                                                                                                                       >  1:732612/1‑150 (MQ=255)

 aGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAACCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTg                                                                                                                                                      <  2:3098825/150‑1 (MQ=255)

  gCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTgg                                                                                                                                                     <  2:1657302/150‑1 (MQ=255)

  gCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTgg                                                                                                                                                     <  1:1771506/150‑1 (MQ=255)

    cTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTg                                                                                                                                                   <  2:1564919/150‑1 (MQ=255)

      gtgCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCa                                                                                                                                                 <  1:2620406/150‑1 (MQ=255)

        gctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAgg                                                                                                                                               <  1:1251100/150‑1 (MQ=255)

        gctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAgg                                                                                                                                               <  1:542557/150‑1 (MQ=255)

        gctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAgg                                                                                                                                               <  2:3294108/150‑1 (MQ=255)

          tgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGAc                                                                                                                                             <  2:1583470/150‑1 (MQ=255)

          tgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGAc                                                                                                                                             <  1:372622/150‑1 (MQ=255)

          tgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCGGATCACCGCCCTGCTGCACGCCAGCCGCTGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGAc                                                                                                                                             <  2:359568/150‑1 (MQ=255)

           gcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCTCCTGCACCAGGCGCAGGGTGCAggggc                                                                                                                                            <  1:2874158/150‑4 (MQ=255)

              gCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGa                                                                                                                                         <  1:2244846/150‑1 (MQ=255)

              gCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGa                                                                                                                                         >  2:2050950/1‑150 (MQ=255)

                cAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGc                                                                                                                                       <  2:1449504/150‑1 (MQ=255)

                cAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGc                                                                                                                                       <  2:5034247/150‑1 (MQ=255)

                          ccTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                             <  2:2786507/150‑1 (MQ=255)

                          ccTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                             <  2:59862/150‑1 (MQ=255)

                              cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                         >  1:4695680/1‑150 (MQ=255)

                              cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                         >  1:4695660/1‑150 (MQ=255)

                               aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                        <  1:2494787/150‑1 (MQ=255)

                               aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                        >  2:4651416/1‑150 (MQ=255)

                                  ggCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTgg                                                                                                                                                     <  2:4797356/118‑1 (MQ=255)

                                  ggCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTg                                                                                                                     >  1:5029431/1‑150 (MQ=255)

                                    cTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                        <  2:2802917/145‑1 (MQ=255)

                                        cccGAGCAGGCCGAACACAAACTCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCAGAGCGACACCCGGCTGGACCTGTACGAc                                                                                                               >  2:4114734/1‑150 (MQ=255)

                                          cGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAccc                                                                                                             >  2:2225751/1‑150 (MQ=255)

                                           gAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                            <  2:3803991/150‑1 (MQ=255)

                                              cAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                         <  1:4331933/150‑1 (MQ=255)

                                               aGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgc                                                                                                        <  1:3866856/150‑1 (MQ=255)

                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                      >  1:2494131/1‑150 (MQ=255)

                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                      >  1:2493908/1‑150 (MQ=255)

                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                      >  1:1064382/1‑150 (MQ=255)

                                                     aaCACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCa                                                                                                  >  1:1754173/1‑150 (MQ=255)

                                                       cacaAACGCCCGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCGTCGACCCGAGCTAGTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAGCTGTACGACCCCGTGCGCGTCCTc                                                                                                >  2:2497164/1‑150 (MQ=255)

                                                        acaAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATcc                                                                                               <  2:1916102/150‑1 (MQ=255)

                                                            aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcac                                                                                           >  1:4029942/1‑150 (MQ=255)

                                                              gCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcaccc                                                                                         >  2:1728317/1‑150 (MQ=255)

                                                                  ggAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                                     <  2:980789/150‑1 (MQ=255)

                                                                   gAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTg                                                                                    >  2:448635/1‑150 (MQ=255)

                                                                     gCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCt                                                                                  <  1:2050950/150‑1 (MQ=255)

                                                                        gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcga                                                                               >  1:4143333/1‑150 (MQ=255)

                                                                        gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATAGCGACACAAGCCCCTGAACCCTGCGCCTGGTGCAGGACCTGAGCGACACACGGCTGGACCTGTACGACCCCGTGCGCTTCATCAGCACCCACCTGCTcga                                                                               >  2:4420699/1‑150 (MQ=255)

                                                                            aCCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGAAACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgaccga                                                                           >  1:3898944/1‑150 (MQ=255)

                                                                                 ccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                      <  1:2184450/150‑1 (MQ=255)

                                                                                 ccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                      <  2:127158/150‑1 (MQ=255)

                                                                                    gctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGtt                                                                   >  1:1030571/1‑150 (MQ=255)

                                                                                    gctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGtt                                                                   <  1:4990131/150‑1 (MQ=255)

                                                                                     ctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTc                                                                  <  1:4523679/150‑1 (MQ=255)

                                                                                      tgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                                 >  1:754148/1‑150 (MQ=255)

                                                                                      tgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                                 >  1:1258109/1‑150 (MQ=255)

                                                                                       gcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAc                                                                >  2:3490405/1‑150 (MQ=255)

                                                                                        cACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTc                                                                                 >  2:2935919/1‑132 (MQ=255)

                                                                                        cACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTc                                                                                 <  1:2935919/132‑1 (MQ=255)

                                                                                        cACGCCAGCCGCGGAGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGt                                                                                                                    <  1:1526378/97‑1 (MQ=255)

                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAg                                                                                                                                                <  1:5409450/66‑1 (MQ=37)

                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAg                                                                                                                                                >  2:5409450/1‑66 (MQ=37)

                                                                                             cAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                      >  2:4005693/1‑138 (MQ=255)

                                                                                             cAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCa                                                          <  1:3542123/150‑1 (MQ=255)

                                                                                             cAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCa                                                          <  1:3542094/150‑1 (MQ=255)

                                                                                                     gcggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                                  <  1:2122989/150‑1 (MQ=255)

                                                                                                           tCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                               <  2:3895274/147‑1 (MQ=255)

                                                                                                           tCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAg                                            >  2:2999004/1‑150 (MQ=255)

                                                                                                             gACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCt                                          >  2:180010/1‑150 (MQ=255)

                                                                                                               cccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgt                                        <  2:1660954/150‑1 (MQ=255)

                                                                                                                 cGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGACCCGGTAGCTGTGc                                      >  2:3507745/1‑150 (MQ=255)

                                                                                                                 cGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGc                                      >  2:3508252/1‑150 (MQ=255)

                                                                                                                  gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGTCCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCa                                     >  1:1910559/1‑150 (MQ=255)

                                                                                                                  gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCa                                     >  1:3328010/1‑150 (MQ=255)

                                                                                                                   ctCTATTGCGACACACGCCCCGGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCAc                                    <  1:3986572/149‑1 (MQ=255)

                                                                                                                     cTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                                  <  1:456290/150‑1 (MQ=255)

                                                                                                                     cTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                                  <  2:4695660/150‑1 (MQ=255)

                                                                                                                      tATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTg                                 >  1:4835515/1‑150 (MQ=255)

                                                                                                                       aTTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGa                                <  1:204526/150‑1 (MQ=255)

                                                                                                                        ttGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAc                               >  2:1179638/1‑150 (MQ=255)

                                                                                                                          gCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCt                             <  2:533590/150‑1 (MQ=255)

                                                                                                                            gACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgc                           >  1:3352473/1‑150 (MQ=255)

                                                                                                                             acacAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTc                                                                  <  1:4810164/110‑1 (MQ=255)

                                                                                                                              cacaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcag                         >  1:2968986/1‑150 (MQ=255)

                                                                                                                              cacaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcag                         >  1:1226357/1‑150 (MQ=255)

                                                                                                                               acaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                                  >  2:2338770/1‑124 (MQ=255)

                                                                                                                               acaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcagc                        >  2:1396292/1‑150 (MQ=255)

                                                                                                                                 aaGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcac                      >  2:4407694/1‑150 (MQ=255)

                                                                                                                                   gCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccc                    >  1:5168516/1‑150 (MQ=255)

                                                                                                                                   gCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccc                    <  1:2225751/150‑1 (MQ=255)

                                                                                                                                   gCCCCTGCACCCTCCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccc                    <  1:279131/150‑1 (MQ=255)

                                                                                                                                    gtccTGCACCAGGCGCAGGGTGCAGGGCCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccca                   >  2:2984116/3‑150 (MQ=255)

                                                                                                                                    ccccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccca                   >  2:2227182/1‑150 (MQ=255)

                                                                                                                                    ccccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccca                   >  2:1097322/1‑150 (MQ=255)

                                                                                                                                     tccTGCACCAGGCGCAGGGTGCAGGGCCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccag                  >  1:5260013/2‑150 (MQ=255)

                                                                                                                                      ccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTgg                                                                       >  2:2371814/1‑96 (MQ=255)

                                                                                                                                      ccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTgg                                                                       <  1:2371814/96‑1 (MQ=255)

                                                                                                                                        tGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagcac               <  1:2302181/150‑1 (MQ=255)

                                                                                                                                        tGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagcac               >  2:4828419/1‑150 (MQ=255)

                                                                                                                                        tGCACCAGGCGCAGGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagcac               >  2:4705255/1‑150 (MQ=255)

                                                                                                                                        tGCACCAGGCGCAGGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagcac               >  1:3260859/1‑150 (MQ=255)

                                                                                                                                             ccTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg          <  1:3236396/150‑1 (MQ=255)

                                                                                                                                              cTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTg                            >  1:1588146/1‑131 (MQ=255)

                                                                                                                                              cTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGc         <  1:4309587/150‑1 (MQ=255)

CAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAGCC  >  NC_002947/5390704‑5391002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`