BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,420,789	T→A	7.1%	V84V (GTA→GTT) ‡	PP_4758 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,420,789	0	T	A	7.1%	507.1 / 20.1	155	V84V (GTA→GTT) ‡	PP_4758	MFS transporter
Reads supporting (aligned to +/- strand): ref base T (74/70); new base A (5/6); total (79/76)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 155 total aligned reads displayed.

CTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5420641‑5420937

     gCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCg                                                                                                                                                >  2:3688286/1‑150 (MQ=255)

       cACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGaa                                                                                                                                              <  2:3419350/150‑1 (MQ=255)

       cACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGaa                                                                                                                                              >  2:1515836/1‑150 (MQ=255)

         ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                            <  2:53475/150‑1 (MQ=255)

          cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGTCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAg                                                                                                                                           >  2:817270/1‑150 (MQ=255)

           ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgc                                                                                                                                          >  1:4057967/1‑150 (MQ=255)

           ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgc                                                                                                                                          <  1:267919/150‑1 (MQ=255)

           ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgc                                                                                                                                          <  1:267912/150‑1 (MQ=255)

                  gAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAc                                                                                                                                   >  2:4907022/1‑150 (MQ=255)

                  gAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAc                                                                                                                                   <  1:776394/150‑1 (MQ=255)

                   aaaaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAcc                                                                                                                                  >  2:1866561/1‑150 (MQ=255)

                     aaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGa                                                                                                                                >  2:5280908/1‑150 (MQ=255)

                       cGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATg                                                                                                                              <  1:853654/150‑1 (MQ=255)

                       cGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATg                                                                                                                              <  1:853661/150‑1 (MQ=255)

                        gcagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATga                                                                                                                             >  2:252991/1‑150 (MQ=255)

                        gcagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATga                                                                                                                             >  2:3917137/1‑150 (MQ=255)

                         cagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaa                                                                                                                            <  1:3917137/150‑1 (MQ=255)

                         cagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaa                                                                                                                            <  2:1263570/150‑1 (MQ=255)

                          agcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaag                                                                                                                           <  1:3688286/150‑1 (MQ=255)

                             aCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAAc                                                                                                                        <  2:3915685/150‑1 (MQ=255)

                               gTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGCTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAg                                                                                                                      <  2:1581473/150‑1 (MQ=255)

                                tAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGc                                                                                                                     >  2:2796134/1‑150 (MQ=255)

                                     cTGGGTTTCGTTCTGCACAAAGGCCTTGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGc                                                                                                                <  1:3579224/150‑1 (MQ=255)

                                          tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGa                                                                                                           >  2:720241/1‑150 (MQ=255)

                                           ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                          <  1:669478/150‑1 (MQ=255)

                                           ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                          >  2:2876920/1‑150 (MQ=255)

                                             cGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGc                                                                                                        <  1:1210611/150‑1 (MQ=255)

                                               ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCa                                                                                                      >  1:1470553/1‑150 (MQ=255)

                                                 cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                    <  2:3613790/150‑1 (MQ=255)

                                                   gCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAgg                                                                                                  >  1:974752/1‑150 (MQ=255)

                                                   gCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAgg                                                                                                  <  2:3683824/150‑1 (MQ=255)

                                                       aaaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGc                                                                                              >  1:115315/1‑150 (MQ=255)

                                                          ggCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgc                                                                                           <  1:4162567/150‑1 (MQ=255)

                                                            ccATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAgcgc                                                                                         <  2:4722192/150‑1 (MQ=255)

                                                             cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        >  2:4216322/1‑150 (MQ=255)

                                                             cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        >  2:1469126/1‑150 (MQ=255)

                                                             cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        >  1:5190630/1‑150 (MQ=255)

                                                             cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        <  1:1255539/150‑1 (MQ=255)

                                                               tGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGa                                                                                      >  1:4952848/1‑150 (MQ=255)

                                                                ggCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGAt                                                                                     <  2:1287733/150‑1 (MQ=255)

                                                                ggCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGAt                                                                                     <  2:4194610/150‑1 (MQ=255)

                                                                  ccGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGc                                                                                   >  1:4180231/1‑150 (MQ=255)

                                                                    gTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGccc                                                                                 <  2:2428700/150‑1 (MQ=255)

                                                                     tGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGa                                                                                                                                >  1:784524/1‑102 (MQ=255)

                                                                     tGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGa                                                                                                                                <  2:784524/102‑1 (MQ=255)

                                                                            caGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCt                                                                         >  1:2190045/1‑150 (MQ=255)

                                                                                 cccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCa                                                                    >  1:3190188/1‑150 (MQ=255)

                                                                                     ggTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTg                                                                >  1:1441588/1‑150 (MQ=255)

                                                                                        gAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgg                                                             >  1:4836511/1‑150 (MQ=255)

                                                                                         aaCATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcggg                                                            >  1:4759101/1‑150 (MQ=255)

                                                                                          aCATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggc                                                           <  2:1124496/150‑1 (MQ=255)

                                                                                              gATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGAc                                                       >  1:2446143/1‑150 (MQ=255)

                                                                                              gATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGAc                                                       >  2:5332719/1‑150 (MQ=255)

                                                                                              gATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGAc                                                       >  1:3479705/1‑150 (MQ=255)

                                                                                               aTACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                            <  1:4837242/64‑1 (MQ=38)

                                                                                                tACGGGTAAGCCAAAGCCGGGCACAGACCTTTAGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCAGGCACCGAAGCCATAGGCCGCAGAGCTGAAGCCCAGGTCGCATACCAGCTGCGGGCGGACGa                                                     >  2:2464900/1‑150 (MQ=255)

                                                                                                 aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                    <  2:1431131/150‑1 (MQ=255)

                                                                                                 aCGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGaa                                                    <  2:1431148/150‑1 (MQ=255)

                                                                                                    ggTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGcc                                                 <  1:658184/150‑1 (MQ=255)

                                                                                                      tCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCaa                                               <  2:2268890/150‑1 (MQ=255)

                                                                                                       cAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGcaggc                                              <  2:1993693/150‑5 (MQ=255)

                                                                                                         gCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCAGAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGcccctgt                                            >  2:3287450/1‑145 (MQ=255)

                                                                                                           cacaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTg                                          >  1:3752933/1‑150 (MQ=255)

                                                                                                           cacaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTg                                          >  2:3005044/1‑150 (MQ=255)

                                                                                                             caGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCGGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGAc                                        <  2:4337278/150‑1 (MQ=255)

                                                                                                                   ggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTc                                  <  1:1515836/150‑1 (MQ=255)

                                                                                                                     cACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGa                                >  2:322764/1‑150 (MQ=255)

                                                                                                                     cACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGa                                <  2:5245057/150‑1 (MQ=255)

                                                                                                                      aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               >  2:1184949/1‑150 (MQ=255)

                                                                                                                      aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               <  1:2643473/150‑1 (MQ=255)

                                                                                                                      aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               <  1:4560006/150‑1 (MQ=255)

                                                                                                                      aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               <  1:4560026/150‑1 (MQ=255)

                                                                                                                       ccGACCTTTTGCAACAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAgg                              <  2:1790550/150‑1 (MQ=255)

                                                                                                                        cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACATCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCAAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             >  1:2049725/1‑150 (MQ=255)

                                                                                                                        cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGt                                                                 >  2:936212/1‑114 (MQ=255)

                                                                                                                        cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             >  1:4244569/1‑150 (MQ=255)

                                                                                                                        cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             >  1:50921/1‑150 (MQ=255)

                                                                                                                        cGACCTTTTGCAGCAACATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGt                             >  2:2989209/1‑150 (MQ=255)

                                                                                                                         gACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTa                            >  1:896219/1‑150 (MQ=255)

                                                                                                                         gACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTa                            <  1:128498/150‑1 (MQ=255)

                                                                                                                          aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                           >  1:171655/1‑150 (MQ=255)

                                                                                                                           ccTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAGGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGt                          <  2:5067778/150‑1 (MQ=255)

                                                                                                                             ttttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTg                        >  2:4123120/1‑150 (MQ=255)

                                                                                                                             ttttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTg                        >  2:4589970/1‑150 (MQ=255)

                                                                                                                              tttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGa                       >  1:468327/1‑150 (MQ=255)

                                                                                                                              tttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGa                       <  1:3659937/150‑1 (MQ=255)

                                                                                                                              tttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGa                       <  2:4922661/150‑1 (MQ=255)

                                                                                                                               ttGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGAc                      >  2:3322381/1‑150 (MQ=255)

                                                                                                                                  cagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGAt                   >  2:4010425/1‑150 (MQ=255)

                                                                                                                                  cagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGAt                   >  1:3343661/1‑150 (MQ=255)

                                                                                                                                   agcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATg                  >  1:932786/1‑150 (MQ=255)

                                                                                                                                   agcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATg                  <  2:4662834/150‑1 (MQ=255)

                                                                                                                                   agcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATg                  <  2:533516/150‑1 (MQ=255)

                                                                                                                                         aTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCATGTCGCTTTCCAGGTTCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatg            <  2:2402081/150‑1 (MQ=255)

                                                                                                                                          tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  1:3886331/1‑150 (MQ=255)

                                                                                                                                          tGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           <  1:288656/150‑1 (MQ=255)

                                                                                                                                          tGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  1:4097041/1‑150 (MQ=255)

                                                                                                                                            ttCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTACCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgatg         >  1:2171671/1‑150 (MQ=255)

                                                                                                                                             tCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACAtgatga        <  1:2949602/150‑1 (MQ=255)

                                                                                                                                                   gTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGCCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACa  >  2:4092153/1‑150 (MQ=255)

CTTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5420641‑5420937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`