Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,448,268 A→C 12.7% Q148P (CAG→CCG)  thiE → thiamine‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,448,2680AC12.7% 311.2 / 4.7 149Q148P (CAG→CCG) thiEthiamine‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (85/41);  new base C (10/13);  total (95/54)
Fisher's exact test for biased strand distribution p-value = 3.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

TCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACCCGCCG  >  NC_002947/5448119‑5448415
                                                                                                                                                     |                                                                                                                                                   
atgggCAAGCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCa                                                                                                                                                     >  2:2567934/4‑150 (MQ=255)
 cAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAg                                                                                                                                                    <  2:2584562/150‑1 (MQ=255)
   ggCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGc                                                                                                                                                  <  2:14048/150‑1 (MQ=255)
   ggCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCAGCTCGGGCAGGc                                                                                                                                                  >  2:1095285/1‑150 (MQ=255)
    gCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCCATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCCGGcc                                                                                                                                                 >  1:1222961/1‑150 (MQ=255)
      aaTCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCcg                                                                                                                                               >  2:3885727/1‑150 (MQ=255)
       atcatcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCAGCTCGAGCAGGTCcgc                                                                                                                                              >  1:4244506/1‑150 (MQ=255)
           tcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGccc                                                                                                                                          >  2:873745/1‑150 (MQ=255)
           tcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGccc                                                                                                                                          <  2:4395926/150‑1 (MQ=255)
             ggCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCACCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAg                                                                                                                                        >  2:841515/1‑150 (MQ=255)
                tCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTg                                                                                                                                     >  1:4907676/1‑150 (MQ=255)
                  cACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGaa                                                                                                                                   >  2:4859947/1‑150 (MQ=255)
                    ccTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAAc                                                                                                                                 >  2:5114503/1‑150 (MQ=255)
                    ccTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCCAGCAGGCCCCCCCCCAGGTGAAAc                                                                                                                                 >  2:5114531/1‑150 (MQ=255)
                     cggCCACGCCTGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACt                                                                                                                                <  2:4138786/148‑1 (MQ=255)
                     ccgCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGGTCGAGCAGGCCCGCGCCCCGGTGataaa                                                                                                                                >  1:1482747/3‑146 (MQ=255)
                     cTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCCGGCCCCCGCCCAGGTGGAAcg                                                                                                                                >  1:838607/1‑149 (MQ=255)
                          aCGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGGGAAACTGCCGa                                                                                                                           >  2:5346899/1‑150 (MQ=255)
                          aCGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCCCGCCCCGGGGCAACGGCCgc                                                                                                                           >  1:3947144/1‑149 (MQ=255)
                            gCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATc                                                                                                                         >  1:4856624/1‑150 (MQ=255)
                               aGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGcc                                                                                                                      >  1:4084520/1‑150 (MQ=255)
                               aGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCCCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGcc                                                                                                                      <  2:1202986/150‑1 (MQ=255)
                                 ccTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGGCGGCGCGGGCAGGCCCGCGCCCCCGGGAAAATGCCGAACGccca                                                                                                                    >  1:1148621/1‑148 (MQ=255)
                                  cTCGAACTGGCCGCCCGGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCCGGCCCGCGCCCCGGTGGAACAGCCGCTCGCCGTg                                                                                                                   >  1:2145039/1‑150 (MQ=255)
                                  cTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTg                                                                                                                   >  2:1503799/1‑150 (MQ=255)
                                  cTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGGGGAACTGCCGATCGCCggg                                                                                                                   >  2:109795/1‑148 (MQ=255)
                                  cTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGACACCAAGCCGGGCGCGCCCGCAGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCACGTGAAACTGCCCAACGACGTg                                                                                                                   >  2:2465022/1‑150 (MQ=255)
                                   tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGCGCCCGCCCGCGCCCCGGGGCAACTGCCGAGCGCCGGGa                                                                                                                  >  1:390839/1‑150 (MQ=255)
                                       aCTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCACGTGAAACTGCCGATCGCCGTGATCgg                                                                                                              >  2:357121/1‑150 (MQ=255)
                                       aCTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGCCACCAAGCCGGGCGCGCCCGCGGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCgg                                                                                                              <  2:3427208/150‑1 (MQ=255)
                                         tGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCGGGAACgggg                                                                                                            >  1:1763680/1‑148 (MQ=255)
                                           gccgccCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGTCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCCCCCCCACGTGAAAATGCCGCTCGCCCTGGTCGGTGGc                                                                                                          >  1:1431828/1‑150 (MQ=255)
                                              gccCAGGCTGCCAGCGAAGGCGCCAGCTCCGGGGCCTTTGGCCGCTTCTTCAATCCTGACACCACGCCGGGCGCGCCCGCCGCCACCGTCGCCCTGCGCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATc                                                                                                       <  1:281406/150‑1 (MQ=255)
                                               cccAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCCCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCa                                                                                                      <  2:4524800/150‑1 (MQ=255)
                                                 cAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTGCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCCGGTGAACCTGCCGATCGCCGTGATCGGTGGccgcccc                                                                                                    >  2:4397880/1‑144 (MQ=255)
                                                   ggCTGCCCGCGCGGGCGCCCGCTCGGTGGCCTTGGGCCGATTCTACACTTCTGCCCCCCCGCCGGGCGCGCCCGCGGCCCCCGGCGGCCGGCCCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCt                                                                                                  <  1:3905771/150‑1 (MQ=255)
                                                    gCTGCCCGCGATGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCATTTCTGTCACCACGCCGGGGGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTc                                                                                                 <  2:390839/150‑1 (MQ=255)
                                                    gCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTc                                                                                                 >  2:4513726/1‑150 (MQ=255)
                                                      tGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCCCCACGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGa                                                                                               <  2:178492/150‑1 (MQ=255)
                                                        ccAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGCGAAACAGCCGATCGCCGGGATCGGTGGCATCACCCTCGACa                                                                                             >  2:70412/1‑150 (MQ=255)
                                                         cAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGCGCCGGCCCCCCCCCCCGGGGCACAGCCCCTCGCCGGGATCGGTGGCATCACCCACGACaa                                                                                            >  1:2927347/1‑150 (MQ=255)
                                                           gCGACGGCGCCCGCTAGGGGGCCTTGGGCCGCTTCTTCATTTCTGACACCCCGCGGGGCGCGCCCGCGGCCACCGGCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcg                                                                                          <  1:2157535/150‑1 (MQ=255)
                                                           gCGAAGGCGCCAGCTCCGTGGCCTTTGGCCGCTTCTTCAATTCGGCCACCACGCCGGGCGCGCCCGCGGCCACCGGCGGCGTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcg                                                                                          <  2:1153200/150‑1 (MQ=255)
                                                              aaGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCCGGCCCGCCCCCCCGGGGAACAGCCGATCGCCCGGATCGGTGGCCTCACCCACCACAAccccc                                                                                       >  1:2906506/1‑149 (MQ=255)
                                                                      aGCTACGTGGCCTTTGGCCGCTTCTGCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAACTGCCGCTCGCCGGGGGCGGTGGCATCACCCGCGACCACCCCGccccccag                                                                               >  2:1839098/1‑146 (MQ=255)
                                                                       gCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCACGACCAGGCCCGCGCCCAGGTGAAACTGCCGAAAGCCGTGAGCGGTGGCCTCACCCACGACAACGCCGCCCCCCTgg                                                                              >  2:1118819/1‑150 (MQ=255)
                                                                        cTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGt                                                                             >  2:4401879/1‑150 (MQ=255)
                                                                          ccGTGGCCTTTGGCCGCTTCTTCAATTCTGTCCCCCCCCCGGGCGCGCCCGCGGCCCCCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCg                                                                           <  2:611281/149‑1 (MQ=255)
                                                                            gTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGCTCGGTGGCATCACCCACGACAACGCCCCCCCACTGGTCGcc                                                                         >  2:4988941/1‑150 (MQ=255)
                                                                                    tGGCCGCTTCTTAATTTCGGTCACCATGCGGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGgcgc                                                                 <  1:3159263/150‑1 (MQ=255)
                                                                                       ccGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCTCCCCACAGGTCGCCCACGGCGCCGa                                                              >  2:3367066/1‑150 (MQ=255)
                                                                                        cGCTTCTTCAATACGGTCACCAAGCCGGGCGCGCCCGCTGCCAACGACGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGAc                                                             <  2:3619060/150‑1 (MQ=255)
                                                                                             cttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGGGAAACAGCCCATCGCCGTGATCCGTGGCATCACCCACGACAACGCCCCCCCACAGGTCGCCCACGGCGCCGACctgct                                                        >  2:5026977/1‑150 (MQ=255)
                                                                                              ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGTCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACctgctg                                                       >  2:1424420/1‑150 (MQ=255)
                                                                                              ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCGCCCCCGGGAAACTGCCCATAGCCCTGATCGGTGGCCGCACCCGCGACACCCCCGCCCCCCAGGTCGCCCCCCGCGCCCCCcggctg                                                       >  1:562738/1‑150 (MQ=255)
                                                                                              ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCGCCCACGTGAAAATGCCGATCGCCGTGATCGGTGGCATCACCCACGACAAAGCCGCCCCACAGGTCGCCCACGGCGCCGCCctgctg                                                       >  1:1150732/1‑150 (MQ=255)
                                                                                              ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCACGCCCGCGCCCACGGGAAACTGCCGATCGCCCTGATCGCTGGCAACACCCTCGACAACCCCCCCCCACAGGTCGCCCACCGCGCCCAccaccag                                                       >  1:3358983/1‑145 (MQ=255)
                                                                                              ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACCCCCCCCCACAGGTCGCCCACCGCGCCCACcggcag                                                       >  1:4413647/1‑148 (MQ=255)
                                                                                              ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCAGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGCTCGGTGGCATCACCCTCGACAACGCCGCCCCACAGGTCGCCCACGGCGCCGACctgcag                                                       >  2:4174197/1‑148 (MQ=255)
                                                                                               tcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTgg                                                      >  1:4936542/1‑150 (MQ=255)
                                                                                                  ttccTGCCACCAAGCCGGGCGCGCCCGCTGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCCCTGGTCGCCCACGGCGCCGACCTGCTGGCgg                                                   <  2:2320625/147‑1 (MQ=255)
                                                                                                        tCACCCCGCCGGGCGCGCCCGCGGCCCCCGCCGGCCGGCCCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATcc                                             <  1:3784303/150‑1 (MQ=255)
                                                                                                          aCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                           >  2:4978692/1‑150 (MQ=255)
                                                                                                          aCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATAGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                           >  2:1323140/1‑150 (MQ=255)
                                                                                                          aCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATAGGTGGCATCACCCACGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCAGCTGGCGGTGATCCAc                                           >  1:2852621/1‑150 (MQ=255)
                                                                                                          aCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCCCGCCCGCGCCCAGGTGAAACAGCCGATCGCCGTGATCGGTGGCCTCACCCTCGCCAACGCCGCCCCACAGGTCGCCCGCGGCGCCGACCTGCGGGCGGTGATCCAc                                           >  1:1585349/1‑150 (MQ=255)
                                                                                                           cccccccGCGCGCCCCCGCCGCCACCGCCGCCCGGCTCGCGCCGCCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACg                                          <  1:679128/145‑1 (MQ=255)
                                                                                                           ccAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACg                                          >  2:291961/1‑150 (MQ=255)
                                                                                                           ccAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACg                                          >  2:2699758/1‑150 (MQ=255)
                                                                                                           ccAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACg                                          >  2:4910624/1‑150 (MQ=255)
                                                                                                            cacgCCGGGCGCGCCCGCTGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACgg                                         <  2:4413647/147‑1 (MQ=255)
                                                                                                            cAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGTATCACCCGCGACAACGCCGCCCCACTGGTCGCCCACCGCGCCGACCTGCTGGCGGTGATCAACgg                                         >  1:249999/1‑150 (MQ=255)
                                                                                                            cAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCAGGCCCGCGCCCCGGTGCAACTGCCGCTCGCCGGGATCGGTGGCATCACCCGCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCAGCTGGCGGTGATCCACgg                                         >  1:29858/1‑150 (MQ=255)
                                                                                                             aaGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCGCGGc                                        >  1:378979/1‑150 (MQ=255)
                                                                                                              aGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCGTGATCGGTGGCATCACCCACCACAACGCCGCCCCACAGGTCGCCCACCGCGCCGCCCGGCTGGCGGGGATCCACGGCt                                       >  1:3304088/1‑150 (MQ=255)
                                                                                                               gCCGGGCGCGCCCGCGGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCtt                                      <  2:1148621/150‑1 (MQ=255)
                                                                                                               gCCGGGCGCGCCCGCCGCCTCCGTCGGCCCGCTCGAGCAGGCCCGCGCCAAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCTCCGACCTGCTGGCGGTGATCCACGGCtt                                      <  1:3399273/150‑1 (MQ=255)
                                                                                                                  gggCGCGCCCGCTGCCAACGTCGGCGGGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGtt                                   <  1:2607328/150‑1 (MQ=255)
                                                                                                                    gcgcgcCCGCGGCCCACGTCGGCCTGCTCGCCCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCg                                 <  1:2791793/150‑1 (MQ=255)
                                                                                                                        gcCCGCTCCCCCCGGGGGCCTCCCCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGc                             <  1:81146/150‑1 (MQ=255)
                                                                                                                         cccGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGcc                            <  1:4994272/150‑1 (MQ=255)
                                                                                                                          ccGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGAGCGCCGGGAGCGGTGGCATCACCCGCGACAACGCCGCCCCCCGGGTCGCCCACGGCGCCGACCGGCGGGCGGTGATCCACGGCTTGGTCGGTGCCg                           >  1:1863146/1‑150 (MQ=255)
                                                                                                                           cGCTGCCAACGTCGGCCTGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGa                          <  1:873745/150‑1 (MQ=255)
                                                                                                                                 cAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                    >  1:491736/1‑150 (MQ=255)
                                                                                                                                 cAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                    >  2:3782488/1‑150 (MQ=255)
                                                                                                                                 cAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                    >  2:3782336/1‑150 (MQ=255)
                                                                                                                                 cAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                    >  2:5395816/1‑150 (MQ=255)
                                                                                                                                 cAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCGTGTTCGGTGCCGACAgcgc                    >  2:3809510/1‑150 (MQ=255)
                                                                                                                                 cAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCGCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                    >  2:2407164/1‑150 (MQ=255)
                                                                                                                                  aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg                   >  1:627224/1‑150 (MQ=255)
                                                                                                                                   aCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcgc                  >  1:5090200/1‑150 (MQ=255)
                                                                                                                                    cGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCa                 >  1:1490841/1‑150 (MQ=255)
                                                                                                                                        ggCGTGCTCCCGCCGGGCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGACGACTGCGCTCAGGaa             <  2:1197675/150‑1 (MQ=255)
                                                                                                                                          ccTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGt                               >  2:1077028/1‑130 (MQ=255)
                                                                                                                                          ccTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGt                               <  1:1077028/130‑1 (MQ=255)
                                                                                                                                            tGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCa         >  2:2885265/1‑150 (MQ=255)
                                                                                                                                              cTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCGTGTTCGGTGCCGACAGCGCGCAGGAAGTCAcc       >  2:154100/1‑150 (MQ=255)
                                                                                                                                               tCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAccc      >  2:1127390/1‑150 (MQ=255)
                                                                                                                                                 gAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACccgc    >  2:507882/1‑150 (MQ=255)
                                                                                                                                                   gCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGACGTCACccgccg  >  2:3948448/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACCCGCCG  >  NC_002947/5448119‑5448415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑