Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,510,775 C→A 7.7% S137* (TCG→TAG) ‡ urtA → urea ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,510,7750CA 7.7% 550.4 / 26.5 171S137* (TCG→TAG) ‡urtAurea ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (93/64);  new base A (7/6);  total (100/71)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 172 total aligned reads displayed.

TGAAAAGAGCCGTCAACTGCTGACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATCCTG  >  NC_002947/5510626‑5510923
                                                                                                                                                     |                                                                                                                                                    
tGAAAAGAGCCGTCAACTGCTGACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTc                                                                                                                                                      <  2:3568342/150‑1 (MQ=255)
 gAAAAGAGCCGTCAACTGCTGACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTCCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCg                                                                                                                                                     <  1:1978327/150‑1 (MQ=255)
              aaCTGCTGACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCt                                                                                                                                        >  1:3586969/1‑150 (MQ=255)
                 tgctgACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTaca                                                                                                                                     >  2:2090652/1‑150 (MQ=255)
                   ctgACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTAcaca                                                                                                                                   <  2:4777762/150‑1 (MQ=255)
                    tgACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAg                                                                                                                                  >  2:2560809/1‑150 (MQ=255)
                    tgACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAg                                                                                                                                  >  1:2256943/1‑150 (MQ=255)
                      aCCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGgc                                                                                                                                >  2:1234492/1‑150 (MQ=255)
                        ccAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGgcgc                                                                                                                              <  2:3716216/150‑1 (MQ=255)
                        ccAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGgcgc                                                                                                                              >  2:1802853/1‑150 (MQ=255)
                             aCAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGccc                                                                                                                         <  2:4386524/150‑1 (MQ=255)
                                  gTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAAc                                                                                                                    <  2:3607468/150‑1 (MQ=255)
                                   tGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAAcc                                                                                                                   >  1:1300329/1‑150 (MQ=255)
                                    ggCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCa                                                                                                                  <  2:1025797/150‑1 (MQ=255)
                                       cGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCa                                                                                                               >  2:2079276/1‑150 (MQ=255)
                                       agccgtgtTCGGTTGCTGGACGTCGGTGTCGCGCAAATCGGTTCTCCCGGTCTTCAAGGAACTCAACAATATTCTGTTCTACCCGGTGCAGTATGAAGGTGAGGAATCCGAGCGCAACGTTTTCTATACGGGCGCGGCACCGAACCAGCa                                                                                                               <  1:1709933/146‑1 (MQ=255)
                                            tgtTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCa                                                                                                          >  2:4687177/1‑150 (MQ=255)
                                                  gttgctgGACGTCGGTGTCGCGCAAATCGGTTCTCCCGGTCTTCAAGGAACTCAACAATATTCTGTTCTACCCGGTGCAGTATGAAGGTGAGGAATCCGAGCGCAACGTTTTCTATACGGGCGCGGCACCGAACCAGCAGGCAATccctg                                                                                                    >  2:272999/3‑148 (MQ=255)
                                                      ctgGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGt                                                                                                <  1:4771909/150‑1 (MQ=255)
                                                         gACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGa                                                                                             >  1:2383576/1‑150 (MQ=255)
                                                         gACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGa                                                                                             >  1:5422223/1‑150 (MQ=255)
                                                            aTCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGTCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGACGCCCCGAACCAGCAAGCCATCCCGGCCTTGGAATa                                                                                          >  1:786003/1‑150 (MQ=255)
                                                            aTCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATa                                                                                          >  2:4277400/1‑150 (MQ=255)
                                                            aTCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATa                                                                                          >  1:785913/1‑150 (MQ=255)
                                                              cggtgtCGCGCAAATCGGTTCTCCCGGTCTTCAAGGAACTCAACAATATTCTGTTCTACCCGGTGCAGTATGAAGGTGAGGAATCCGAGCGCAACGTTTTCTATACGGGCGCGGCACCGAACCAGCAGGCAATCCCTGCTGTTGACTatc                                                                                        >  1:1093673/3‑148 (MQ=255)
                                                              ccGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATAcc                                                                                        >  1:702789/1‑150 (MQ=255)
                                                                  gtCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCtgat                                                                                    <  1:4450277/150‑1 (MQ=255)
                                                                   tCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCtgatg                                                                                   >  1:1081113/1‑150 (MQ=255)
                                                                   tCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCtgatg                                                                                   >  1:3567681/1‑150 (MQ=255)
                                                                     gcgcAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGACTACCTGATGAg                                                                                 >  2:3932390/1‑150 (MQ=255)
                                                                     gcgcAAATCGGTTCTCCCGGTCTTCAAGGAACTCAACAATATTCTGTTCTACCCGGTGCAGTATGAAGGTGAGGAATCCGAGCGCAACGTTTTCTATACGGGCGCGGCACCGAACCAGCAGGCAATCCCTGCTGTTGACTATCtgatgga                                                                                 >  2:3297827/1‑148 (MQ=255)
                                                                      cgcAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGc                                                                                <  1:4807774/150‑1 (MQ=255)
                                                                        cAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGa                                                                              <  1:2208667/150‑1 (MQ=255)
                                                                         aaGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAg                                                                             >  1:4122523/1‑150 (MQ=255)
                                                                              tGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGAcgg                                                                        <  1:1165634/150‑1 (MQ=255)
                                                                                  cTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACggcggc                                                                    >  1:2682595/1‑150 (MQ=255)
                                                                                       ggTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAgcgc                                                               <  1:2945204/150‑1 (MQ=255)
                                                                                           ttCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAg                                                           >  1:2205795/1‑150 (MQ=255)
                                                                                           ttCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAg                                                           >  1:4994043/1‑150 (MQ=255)
                                                                                             cGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCa                                                             <  1:1581292/146‑1 (MQ=255)
                                                                                             cGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCa                                                             >  2:1581292/1‑146 (MQ=255)
                                                                                              gaagaaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAgcgc                                                        >  1:4314188/1‑150 (MQ=255)
                                                                                              gaagaaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAgcgc                                                        >  2:1001732/1‑150 (MQ=255)
                                                                                              gaagaaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAgcgc                                                        >  1:3519293/1‑150 (MQ=255)
                                                                                              gaagaaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAgcgc                                                        >  1:2804754/1‑150 (MQ=255)
                                                                                               aagaaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGct                                                       >  1:3089163/1‑150 (MQ=255)
                                                                                               aagaaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGct                                                       <  1:1677545/150‑1 (MQ=255)
                                                                                                 gaaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGcttc                                                     >  1:4942008/1‑150 (MQ=255)
                                                                                                  aaCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGAGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCTTGGAATACCAGATGAGCGAGGACGGCGGCAGCGCCAAGCGcttct                                                    >  1:5303415/1‑150 (MQ=255)
                                                                                                   aCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGcttctt                                                   >  2:787686/1‑150 (MQ=255)
                                                                                                   aCTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGcttctt                                                   >  2:4087941/1‑150 (MQ=255)
                                                                                                    cTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTATACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCttcttc                                                  <  1:3557888/150‑1 (MQ=255)
                                                                                                     tCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCa                                                                   >  2:4805207/1‑132 (MQ=255)
                                                                                                     tCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTcc                                                 >  1:3821592/1‑150 (MQ=255)
                                                                                                       aaCGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAgcgc                                                        >  1:101661/1‑141 (MQ=255)
                                                                                                        aCGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCaa                                                                                                              >  1:2916192/1‑86 (MQ=255)
                                                                                                        aCGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCaa                                                                                                              <  2:2916192/86‑1 (MQ=255)
                                                                                                           gCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGt                                                                                                <  1:5380444/97‑1 (MQ=255)
                                                                                                           gCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGt                                                                                                >  2:5380444/1‑97 (MQ=255)
                                                                                                              tgctgTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCa                                        <  2:3190422/150‑1 (MQ=255)
                                                                                                               gctgTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCAc                                       <  2:4942008/150‑1 (MQ=255)
                                                                                                                  gTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGa                                    <  2:79715/150‑1 (MQ=255)
                                                                                                                   aacTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGAc                                   >  1:3427843/3‑150 (MQ=255)
                                                                                                                      tACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGTGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTAc                                >  2:2280039/1‑150 (MQ=255)
                                                                                                                      tACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTAc                                <  2:3430674/150‑1 (MQ=255)
                                                                                                                      tACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTAc                                >  2:3626385/1‑150 (MQ=255)
                                                                                                                      tACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTAc                                >  1:134509/1‑150 (MQ=255)
                                                                                                                      tACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTAc                                >  2:5023166/1‑150 (MQ=255)
                                                                                                                      tACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTAc                                >  2:2507104/1‑150 (MQ=255)
                                                                                                                      tACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTAc                                >  1:3430674/1‑150 (MQ=255)
                                                                                                                        cccGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCctgctg                                            <  1:3091124/136‑1 (MQ=255)
                                                                                                                        cccGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCctgctg                                            >  2:3091124/1‑136 (MQ=255)
                                                                                                                        cccGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGt                              <  2:4195718/150‑1 (MQ=255)
                                                                                                                        cccGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGt                              >  1:849550/1‑150 (MQ=255)
                                                                                                                         ccGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTa                                 >  2:2342982/1‑146 (MQ=255)
                                                                                                                         ccGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTa                                 <  1:2342982/146‑1 (MQ=255)
                                                                                                                         ccGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTc                             <  2:1668118/150‑1 (MQ=255)
                                                                                                                           ggTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTa                           >  2:3654870/1‑150 (MQ=255)
                                                                                                                            gTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTAc                          >  2:635637/1‑150 (MQ=255)
                                                                                                                            gTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTAc                          <  1:2507104/150‑1 (MQ=255)
                                                                                                                                aGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCAc                      >  1:2470191/1‑150 (MQ=255)
                                                                                                                                 gTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACg                     >  1:1559010/1‑150 (MQ=255)
                                                                                                                                  tATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGc                    <  1:3436776/150‑1 (MQ=255)
                                                                                                                                     gAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGcacc                 >  2:3281949/1‑150 (MQ=255)
                                                                                                                                     gAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCACGAACCAGCAAGACATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGAGCTTCTTCATGATGGGCACCGACTACGTCTACCCACGcacc                 >  1:1777479/1‑150 (MQ=255)
                                                                                                                                       gggCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGcaccac               <  1:1234492/150‑1 (MQ=255)
                                                                                                                                           gAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCCTCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCCGCGCCCAGCGCTTCTTCCTGCTGGGCCCCGACTACGTCTACCCACGCCCCACcaac           >  2:3815136/1‑150 (MQ=255)
                                                                                                                                            aaGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACcaaca          <  1:794008/150‑1 (MQ=255)
                                                                                                                                            aaGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACcaaca          <  1:3696854/150‑1 (MQ=255)
                                                                                                                                              gagaTGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAg        <  1:3313171/150‑1 (MQ=255)
                                                                                                                                              gagaTGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCAAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAg        >  1:5130429/1‑150 (MQ=255)
                                                                                                                                                 aTGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATc     <  1:2090652/150‑1 (MQ=255)
                                                                                                                                                  tGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATcc    <  1:573265/150‑1 (MQ=255)
                                                                                                                                                   gTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATCCt   <  2:820805/150‑1 (MQ=255)
                                                                                                                                                   gTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATCCt   <  2:3159329/150‑1 (MQ=255)
                                                                                                                                                   gTCGCAGATCGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATctt   <  2:4316618/150‑3 (MQ=255)
                                                                                                                                                    tCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCCTCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCCACAAGATCCTg  >  2:245208/1‑150 (MQ=255)
                                                                                                                                                    tCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGcc                                                              >  2:2671284/1‑90 (MQ=255)
                                                                                                                                                    tCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATCCTg  <  2:3433527/150‑1 (MQ=255)
                                                                                                                                                    tCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCAAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATCCTg  <  2:5130429/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGAAAAGAGCCGTCAACTGCTGACCCAGGACAAAGTGGCCGTGGTGTTCGGCTGCTGGACATCCGTGTCGCGCAAGTCTGTTCTGCCGGTGTTCGAAGAACTCAACGGCCTGCTGTTCTACCCGGTGCAGTATGAGGGCGAAGAGATGTCGCCGAACGTGTTCTACACAGGCGCCGCCCCGAACCAGCAAGCCATCCCGGCCGTGGAATACCTGATGAGCGAGGACGGCGGCAGCGCCAAGCGCTTCTTCCTGCTGGGCACCGACTACGTCTACCCACGCACCACCAACAAGATCCTG  >  NC_002947/5510626‑5510923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: