BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	418,298	C→G	6.0%	L26L (CTC→CTG)	PP_0345 →	heptose kinase WapP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	418,298	0	C	G	6.0%	606.4 / 20.4	183	L26L (CTC→CTG)	PP_0345	heptose kinase WapP
Reads supporting (aligned to +/- strand): ref base C (94/78); new base G (3/8); total (97/86)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 183 total aligned reads displayed.

TGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCAAA  >  NC_002947/418151‑418446

  ctCGCCTGGCTGGAGCGGAAGTCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTGTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTTGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                                  <  2:2602584/150‑1 (MQ=255)

   tCGCCTGGCTGGAGCGCAAGGCCCAGACACTCTCCGCTCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACGGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAggg                                                                                                                                                 <  2:4349522/150‑1 (MQ=255)

   tCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAggg                                                                                                                                                 <  2:227915/150‑1 (MQ=255)

        tggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAc                                                                                                                                            >  1:1924950/1‑150 (MQ=255)

          gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                                          <  1:1275957/150‑1 (MQ=255)

           ctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGc                                                                                                                                         <  2:1701362/150‑1 (MQ=255)

            tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCt                                                                                                                                        >  1:449605/1‑150 (MQ=255)

             ggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTg                                                                                                                                       >  2:5064215/1‑150 (MQ=255)

              gAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                      <  1:4399255/150‑1 (MQ=255)

              gAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                      <  2:199786/150‑1 (MQ=255)

                    aaGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcg                                                                                                                                >  1:363752/1‑150 (MQ=255)

                         ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccg                                                                                                                           >  2:2741938/1‑150 (MQ=255)

                         ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccg                                                                                                                           >  1:230759/1‑150 (MQ=255)

                         ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccg                                                                                                                           >  1:4776220/1‑150 (MQ=255)

                         ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCGGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGTCTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccg                                                                                                                           <  2:3671527/150‑1 (MQ=255)

                             aaaCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCa                                                                                                                       <  2:4110684/150‑1 (MQ=255)

                               aCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGt                                                                                                                     <  2:4341092/150‑1 (MQ=255)

                                     cGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTg                                                                                                               >  2:4432966/1‑150 (MQ=255)

                                      gATCGCAAGCAACGCTATTGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTgg                                                                                                              >  1:3231833/1‑150 (MQ=255)

                                      gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTgg                                                                                                              >  1:3150451/1‑150 (MQ=255)

                                      gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTgg                                                                                                              >  1:2162008/1‑150 (MQ=255)

                                      gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTgg                                                                                                              >  1:1488119/1‑150 (MQ=255)

                                       aTCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGt                                                                                                             <  1:1983309/150‑1 (MQ=255)

                                        tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTa                                                                                                            >  2:5438449/1‑150 (MQ=255)

                                        tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGAGAAGTGGTa                                                                                                            >  2:4010222/1‑150 (MQ=255)

                                           caagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGaa                                                                                                                                             >  2:2440194/1‑114 (MQ=255)

                                           caagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGc                                                                                                         >  2:2019468/1‑150 (MQ=255)

                                           caagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGc                                                                                                         >  2:2859/1‑150 (MQ=255)

                                                aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGa                                                                                                    >  1:2107558/1‑150 (MQ=255)

                                                aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGa                                                                                                    <  1:1053110/150‑1 (MQ=255)

                                                     tATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTg                                                                                               >  1:2644992/1‑150 (MQ=255)

                                                     tATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTg                                                                                               <  1:569916/150‑1 (MQ=255)

                                                         gggATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACgg                                                                                           >  1:3347954/1‑150 (MQ=255)

                                                         gggATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACgg                                                                                           >  1:3348022/1‑150 (MQ=255)

                                                            aTGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGAt                                                                                        >  1:3556899/1‑150 (MQ=255)

                                                                aCTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                    >  2:3024198/1‑150 (MQ=255)

                                                                aCTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                    <  2:3856765/150‑1 (MQ=255)

                                                                  tctGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAacta                                                                                  >  1:3287055/1‑150 (MQ=255)

                                                                  tctGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAacta                                                                                  >  1:4189544/1‑150 (MQ=255)

                                                                   ctGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAactac                                                                                 <  1:903114/150‑1 (MQ=255)

                                                                    tGATGGCGAGTTGGACACTGGCGCCGGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAactac                                                                                 >  1:2626033/1‑150 (MQ=255)

                                                                    tGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCa                                                                                                        <  1:4848911/126‑1 (MQ=255)

                                                                    tGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCa                                                                                                        >  2:4848911/1‑126 (MQ=255)

                                                                     gATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAActacta                                                                               <  1:3850618/150‑1 (MQ=255)

                                                                     gATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAActacta                                                                               <  1:3850147/150‑1 (MQ=255)

                                                                      aTGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                                  >  2:2940246/1‑82 (MQ=255)

                                                                      aTGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                                  <  1:2940246/82‑1 (MQ=255)

                                                                      aTGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACtactac                                                                              >  2:1459554/1‑150 (MQ=255)

                                                                      aTGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACtactac                                                                              <  2:5198017/150‑1 (MQ=255)

                                                                       tGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACg                                                                             <  2:4545266/150‑1 (MQ=255)

                                                                         gCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTc                                                                           <  2:3150451/150‑1 (MQ=255)

                                                                           gAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTg                                                                                                                    <  1:166974/107‑1 (MQ=255)

                                                                             gTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAgc                                                                       <  1:155133/150‑1 (MQ=255)

                                                                               tGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAgcgc                                                                     >  2:2163828/1‑150 (MQ=255)

                                                                                ggACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCt                                                                    >  2:4514549/1‑150 (MQ=255)

                                                                                      tGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAAGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCg                                                              <  2:5172983/150‑1 (MQ=255)

                                                                                          gcC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGc                                                          >  2:1297705/1‑150 (MQ=255)

                                                                                            c‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCg                                                        <  1:2549250/150‑1 (MQ=255)

                                                                                            c‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCg                                                        <  1:4670002/150‑1 (MQ=255)

                                                                                                 gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCaa                                                    <  1:5089060/150‑1 (MQ=255)

                                                                                                 gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCaa                                                    <  1:2971593/150‑1 (MQ=255)

                                                                                                 gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCaa                                                    <  1:3379845/150‑1 (MQ=255)

                                                                                                  tACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAg                                                   <  1:3057817/150‑1 (MQ=255)

                                                                                                     gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGcac                                                >  2:325778/1‑150 (MQ=255)

                                                                                                         acCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgc                                            >  2:333264/1‑150 (MQ=255)

                                                                                                           cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGGGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgcgc                                          <  2:5391359/150‑1 (MQ=255)

                                                                                                           cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgcgc                                          <  1:492107/150‑1 (MQ=255)

                                                                                                           cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgcgc                                          >  1:5097368/1‑150 (MQ=255)

                                                                                                            tGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCaa                                                    <  1:2379551/139‑1 (MQ=255)

                                                                                                            tGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGcc                                         <  1:3783438/150‑1 (MQ=255)

                                                                                                            tGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGcc                                         >  2:233820/1‑150 (MQ=255)

                                                                                                              gcggcgGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAactact                                                                                <  2:1254569/109‑1 (MQ=255)

                                                                                                                  cggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTAcc                                   <  2:728478/150‑1 (MQ=255)

                                                                                                                   ggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCt                                  >  2:3896828/1‑150 (MQ=255)

                                                                                                                    gACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTTAACCGCGACCCGCTAAGTGAAGTGGTAAGCATCGAGCGTTACGGGATGAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTg                                 >  1:189784/1‑150 (MQ=255)

                                                                                                                        tCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggc                             >  1:3463123/1‑150 (MQ=255)

                                                                                                                         cGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACCCCGGGGCCGGCAAGCACATGCGCCGGTACCGGGggcg                            >  1:233707/1‑150 (MQ=255)

                                                                                                                         cGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcg                            <  2:1974901/150‑1 (MQ=255)

                                                                                                                         cGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcg                            >  1:1642676/1‑150 (MQ=255)

                                                                                                                          ggCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCGGGggcgg                           >  2:3192593/1‑150 (MQ=255)

                                                                                                                             aGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCa                        >  1:3709713/1‑150 (MQ=255)

                                                                                                                              gCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCAc                       <  2:1488119/150‑1 (MQ=255)

                                                                                                                               ccacGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACg                      >  1:5345066/4‑150 (MQ=255)

                                                                                                                               ccTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCGGGGGCGGCCCCg                      >  2:5418467/1‑150 (MQ=255)

                                                                                                                                cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCCAGCACAAGCGCCGGTACCTGGGGCGGCCACGc                     >  1:941242/1‑150 (MQ=255)

                                                                                                                                 tCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCa                    >  2:953707/1‑150 (MQ=255)

                                                                                                                                   gACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATc                  <  2:474855/150‑1 (MQ=255)

                                                                                                                                   gACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATc                  <  2:668971/150‑1 (MQ=255)

                                                                                                                                   gACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATc                  >  1:1780029/1‑150 (MQ=255)

                                                                                                                                      gcgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCGGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAg               <  2:264593/150‑1 (MQ=255)

                                                                                                                                           gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGa          >  2:2213435/1‑150 (MQ=255)

                                                                                                                                            ttCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGaa         >  2:1128800/1‑150 (MQ=255)

                                                                                                                                            ttCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGaa         <  1:3464157/150‑1 (MQ=255)

                                                                                                                                             tCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt        >  2:4662926/1‑150 (MQ=255)

                                                                                                                                               gCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg                                                            >  1:778079/1‑96 (MQ=255)

                                                                                                                                                  cTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCaa   >  2:4897836/1‑150 (MQ=255)

                                                                                                                                                  cTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCaa   <  1:1128078/150‑1 (MQ=255)

                                                                                                                                                  cTCCAGGACGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCaa   >  1:2695838/1‑150 (MQ=255)

                                                                                                                                                   tCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCaaa  >  2:4209353/1‑150 (MQ=255)

                                                                                                                                                   tCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCaaa  >  2:844303/1‑150 (MQ=255)

TGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCC‑GGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCAAA  >  NC_002947/418151‑418446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`