Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,539,049	C→T	13.5%	intergenic (+56/+47)	nadE → / ← PP_4870	NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,539,049	0	C	T	13.5%	229.0 / 26.2	98	intergenic (+56/+47)	nadE/PP_4870	NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base C (35/45);  new base T (4/9);  total (39/59)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

GCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCGAACTTC  >  NC_002947/5538900‑5539198                                                                                                                                                      |                                                                                                                                                      gCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGc                                                                                                                                                       <  2:1255030/150‑1 (MQ=255)  cATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCa                                                                                                                                                      <  2:4129814/150‑1 (MQ=255)    tGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCAtt                                                                                                                                                    >  2:2639143/1‑150 (MQ=255)       gCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                                                 <  1:567295/150‑1 (MQ=255)       gCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                                                 <  1:3690115/150‑1 (MQ=255)        cAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgcg                                                                                                                                                <  2:1781268/150‑1 (MQ=255)        cAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgcg                                                                                                                                                <  2:1780943/150‑1 (MQ=255)         cgCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                               <  1:5052655/149‑1 (MQ=255)         atctggTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCGGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               <  1:4717351/146‑1 (MQ=25)         aGCCGGTTGACCTGGGAGCGTTCGACATCATCGTCGCCACCTTCCGCAAGACCCAGCTCAAGCGGGAACTGCGGTTCGCTCGTTGATAAGTGGCCTGCTGGAGCGGGTTGACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               <  2:5281009/150‑1 (MQ=18)         aGCCGGTTGACCTGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                               <  1:700047/150‑1 (MQ=255)         aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               <  1:1209940/150‑1 (MQ=25)         aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               <  2:5281221/150‑1 (MQ=25)         aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               <  1:2070572/150‑1 (MQ=25)         aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                               <  2:4639361/150‑1 (MQ=255)         aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                               <  2:3255365/150‑1 (MQ=255)         aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                               <  1:2028869/150‑1 (MQ=255)         aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                               <  1:5052653/150‑1 (MQ=255)          gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCggg                                                                                                                                              <  2:1721282/150‑1 (MQ=25)          gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCCCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                                              >  2:4873279/1‑150 (MQ=255)           ccGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                                             <  1:238850/150‑1 (MQ=255)           ccGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                                             <  1:4969447/150‑1 (MQ=255)           ccGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGGGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                                             <  1:238580/150‑1 (MQ=255)           ccGGTTGACCAGGCAGCGTTCGACAGCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                                             <  1:1169218/150‑1 (MQ=255)              gTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGaa                                                                                                                                          <  1:2395111/150‑1 (MQ=25)                   ccAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCACGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                                                     <  1:2262743/150‑1 (MQ=255)                       gCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCC‑TGATAAGTGGCCTGCAGGAGCGGGGTGACCCGCGGATAAGAGGGTGTGCCCCCCCACCCATTAGCGGGGGGTCCCGCGccag                                                                                                                                >  1:2916845/1‑148 (MQ=255)                         aGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                                              >  1:3829549/1‑135 (MQ=255)                         aGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                                              >  1:3829676/1‑135 (MQ=255)                         aGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                                              <  2:3829676/135‑1 (MQ=255)                         aGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                                              <  2:3829549/135‑1 (MQ=255)                          gctttCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCTGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCa                                                                                                                              <  2:5294301/147‑1 (MQ=255)                           cGTTCGACATCATCGTCGCCACCACCCCCAAGACCCAGCACACGCGCGAACTGCCGTTCGCTCCTTGATACGTGGCCGGCTGGAGGGGGTTCCCCGGCGAATACGCGGGTGAACCCACCGACGAATTCGGGGGTGAACCCGCTCCTGCAg                                                                                                                             <  2:3425047/150‑1 (MQ=255)                              tCGACATCTCCGCCCCCACCTCCCGCCAGACCCAGCACAAGCGCGACCTGCCGTTCGCGCCTGGATTCGTGGCCGGCAGGCGGGGTTTCACCCGCTACTACGTGGGTGAACCCACCGCGGCATGCGGGGTTGACCCCGCTCCTGCAGGGt                                                                                                                          <  2:3559569/150‑1 (MQ=255)                                gACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               <  2:5114290/127‑1 (MQ=21)                                gACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               >  1:5114290/1‑127 (MQ=21)                                     catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCCCGAATAAGAGGGTGAAACCACCGAAGCATTAGCGGGTGGACCCGCACCTGCCGGGTTCGCCGt                                                                                                                   >  2:1668727/1‑150 (MQ=255)                                              cACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATACGTGGCCTGCAGGAGCGGGTTCACCCGCGCAAGCGCGGGTGAACCCACCGACGCCTTCGCGGGTGAACCCGCGCCTGCCGGGGTCGCCGgccgcccgtg                                                                                                          >  2:986959/1‑146 (MQ=255)                                                     cGCAAGACCCAGCACAAGCGCGACCTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATCCGTGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACtt                                                                                                   <  1:4078089/150‑1 (MQ=255)                                                       catgACCCCGCACACGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGTGGGTGAACCCACCGACGCATTCGCGGTTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                                                 <  1:5449944/147‑1 (MQ=38)                                                       cAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               <  2:1274288/104‑1 (MQ=17)                                                       cAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                               >  1:1274288/1‑104 (MQ=17)                                                       cAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCAACCGCGAATACGAGGGTGTACCCACCCACGCATTCGCGGGTGAACCCGCACCTGCAGGGTTCGCCGTCAGCCGGTGATTAATTGa                                                                                                 >  2:1222939/1‑150 (MQ=255)                                                       cAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGGTGACCCGCGAATACGAGGGTGAACCCCCCGACGCAGTCGCGGGTGAACCCGCGCCTGCAGGGTTCGCCGTCAGCCTTTGGTTACGTGa                                                                                                 >  2:4676963/1‑150 (MQ=255)                                                        aaGACCCAGCACACGCGCGACCGGCCGTTCGCTCCTTTATATGTGGCCTGCTGGTGGGGGTTCACCGGCGAATACGCGGGTCAACCCCCCGACGCATCCGCGGGTGACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGAc                                                                                                <  2:921112/150‑1 (MQ=255)                                                              cAGCACACGCGCTAACTGCCTTTCGCTCCTTTATATGTGGCCTGCTGGAGGGGTTTCACCCGCTAATACGAGGGTGAACCCACCGAGGCTTTCGCGGTTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGAc                                                                                          <  2:626301/150‑1 (MQ=255)                                                               aGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGAcc                                                                                         <  2:4940262/150‑1 (MQ=255)                                                                 cacaCGCGCGAAGTGCGGTGCGCTCATTGATATGGGGCGTGCTGGTGGGGGTACACCCGCTAATAGGTGGTTTACCCACCCGACGAATTCGGGGTTTACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCtt                                                                                       <  1:5276240/150‑1 (MQ=255)                                                                 cacaAGCGCTAACTGCCGTTCGCTCTTTTTCATGTGGCCTGCTGGCGGGGGTTCCCCGGCTAATACGTGGGTGAACCCACCGCCGCATTCGCGGGTCAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCtt                                                                                       <  1:3271230/150‑1 (MQ=255)                                                                 cacaAGCGCGAACTGCCGTTCGCTCCTTTATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTTAACCCACCGACGCAGTCGCGGGAGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCtt                                                                                       <  2:4193645/150‑1 (MQ=255)                                                                     cgcgcTAACTGCCTTTCGCTCCTTGATAAGTGCCGTGCTGGAGGGGTTTCACCCGCTAATACGTGGGTTAACCCACCGCCGCATTGGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCt                                                                                   <  1:3963435/149‑1 (MQ=255)                                                                     cgcgcTAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGCGCGGGTTCACCCGCTATTAGTAGGGTGAACCCACCGACGCATTCGGGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCt                                                                                   <  2:1311538/149‑1 (MQ=255)                                                                        gcgAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGGTCACCCGCGCATACCAGGGTGAACCCCCCCCCCCCGTCGCGGGGGAACCCCCGCCAGCAGGGGTCGCCCGCAGCCGTTGATTAAGTGGCGATGACCATGccgatc                                                                                >  2:68006/1‑146 (MQ=255)                                                                          gAAGTGCCGTTCGCTCCTTGATATGTGGCCTGCTGGAGCGGGTTCACCGGCGAATACGTGGTTGAACCCACCGACGCATTCGGGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtcat                                                                              <  2:5185984/150‑1 (MQ=39)                                                                          gAACTGCGGTGCGCTCCTTGATACGTGGCCTGCCGGTGCGGGTTCACCCGCGAATACGCGGGTGACCCCACCGACGCATTCGGGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtcat                                                                              <  2:1097833/150‑1 (MQ=38)                                                                              ggCCGTTCGCTCCTTGATAAGGGGCCTGCAGGCGCGGGTTCACCCGCCAATACGCGGGTGAACCCACCGACGCATTCGCGGGTGACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                                                          <  1:3502319/149‑1 (MQ=38)                                                                               gccttgcGCTCCTTTTTAGGTGGCTTGCCGGAGCGGGTACACCGGGGATTAGTTGGTTGAACCCCCGGACGATTTGGCGGTTGACCCCGCTCCTGCAGGGTTCGCCGTCATCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCg                                                                         <  2:929634/144‑1 (MQ=255)                                                                                ccGTTCGCTCCTTGATAAGTGGCCTGCCGGAGCGGGTTCACCCGCGACTACGCGGGTGAACCCACCGACGCATGCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCga                                                                        <  1:3623836/150‑1 (MQ=255)                                                                                          cTTGATAAGTGGCCTGCAGGAGCGGGGTCACCCGCGAAAACGAGGGTGAACCCCCCGACGCATTAGCGGGTGAACCCCCTCCGGCAGGGGTCGCCCTCAGCCGGTGATTACTTGACGCTGACCGTGCCGTTCATCAACGAGATGTGGccc                                                              >  2:3952404/1‑150 (MQ=255)                                                                                                      ccTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCCCTCCTGCAGGGTTCACCGTCAGCCTTTGTTTACTTGACCATGACCATGCCGTTCATCATCGAGATGTGGCCCGGGAAGGTGCag                                                  >  2:2566713/1‑150 (MQ=37)                                                                                                          ccggAGCGGGTTCACCCGCGAATCCGCGGGTCAACCCACCGCCGCCTGCGCGGGTGACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCagaaga                                              <  2:4126656/148‑1 (MQ=255)                                                                                                           aGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCTTTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAgaagaa                                             <  2:3474228/150‑1 (MQ=255)                                                                                                            ttcgCGGGTGAACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                                            >  1:964018/4‑150 (MQ=255)                                                                                                              tggggtttCACCGGCTAATTCGTGGTTTAACCCACCGACGATTTGGGGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCt                                          <  2:1504980/144‑1 (MQ=255)                                                                                                                gggggTCACCCGCTAATACGGGGGTGACCCCACCGACGCTGTCGGGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGt                                        <  2:4800236/149‑1 (MQ=255)                                                                                                                   gTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt                                     >  2:3097014/1‑150 (MQ=25)                                                                                                                   gTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt                                     >  2:2658610/1‑150 (MQ=255)                                                                                                                    ttCACCCTCGTATTCGCGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTc                                    >  1:1764415/1‑150 (MQ=255)                                                                                                                    ttCACCCTCGTATTCGCGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTc                                    >  1:1501241/1‑150 (MQ=255)                                                                                                                       aCCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacc                                 >  1:5357872/1‑150 (MQ=25)                                                                                                                       aCCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacc                                 >  2:2203105/1‑150 (MQ=255)                                                                                                                         ccGCTAATACTCGGTGGAACCCCCCGACGCTTGCGGGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGGcaccac                               <  1:2624361/150‑1 (MQ=255)                                                                                                                         ccGCGAATCCGCGGGTCAACCCACCGACGCTTTCGCGGGTGAACCCGCTCCTGCAGGGTTCACCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                               <  1:2566713/150‑1 (MQ=255)                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                               >  1:2176005/1‑150 (MQ=255)                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                               >  1:3192215/1‑150 (MQ=255)                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                               >  1:3624479/1‑150 (MQ=255)                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                               >  1:3793567/1‑150 (MQ=255)                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                               >  2:5132451/1‑150 (MQ=255)                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCGTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                               >  1:202571/1‑150 (MQ=255)                                                                                                                               attcgcgggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCtt                         >  1:2486773/7‑150 (MQ=255)                                                                                                                               attcgcgggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCtt                         >  1:5211330/7‑150 (MQ=255)                                                                                                                               aTACGCGGGTGAACCCACCGACGCTTCCGCGGGTCACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCtt                         <  1:3352864/150‑1 (MQ=255)                                                                                                                                   gAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAg                     >  1:2799664/1‑150 (MQ=255)                                                                                                                                   gAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAg                     >  1:2820262/1‑150 (MQ=255)                                                                                                                                   gAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAg                     >  1:351790/1‑150 (MQ=255)                                                                                                                                   gAGGGTGAACACACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAg                     >  1:3048867/1‑150 (MQ=255)                                                                                                                                     gggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCt                   >  2:4397750/1‑150 (MQ=255)                                                                                                                                      ggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCtt                  >  1:66727/1‑150 (MQ=255)                                                                                                                                       gTGACCCCACCGACGCAGACGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTg                 <  1:5294658/150‑1 (MQ=255)                                                                                                                                          accccACCCCCGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGaa              <  1:2042534/148‑1 (MQ=255)                                                                                                                                          aaCCCACCGCCGCATGGGCGGGTGACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGaa              <  1:2028311/150‑1 (MQ=255)                                                                                                                                          aaCCCACCGCCGCACTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGaa              <  2:3133765/150‑1 (MQ=255)                                                                                                                                             ccACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAgtg           >  1:2228142/1‑150 (MQ=255)                                                                                                                                             ccACCGACGAATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAgtg           <  1:3952404/150‑1 (MQ=255)                                                                                                                                               aCCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTc         >  1:4102105/1‑150 (MQ=255)                                                                                                                                               aCCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTGTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTc         >  1:4187658/1‑150 (MQ=255)                                                                                                                                                gaaaagCAATCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCg        <  2:4371675/145‑1 (MQ=255)                                                                                                                                                 cGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCGa       >  2:544247/1‑150 (MQ=255)                                                                                                                                                  gACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCGaa      <  1:3541483/150‑1 (MQ=255)                                                                                                                                                      cATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCGAacttc  <  2:351790/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCGAACTTC  >  NC_002947/5538900‑5539198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑