BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,543,690	T→A	5.1%	I327F (ATC→TTC)	dnaB ←	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,543,690	0	T	A	5.1%	762.0 / 19.1	230	I327F (ATC→TTC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand): ref base T (98/119); new base A (3/9); total (102/128)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 235 total aligned reads displayed.

CAGCTGCGAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTGGCCGG  >  NC_002947/5543541‑5543839

cAGCTGCGAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGAt                                                                                                                                                       >  2:3929610/1‑150 (MQ=255)

 aGCTGCGAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGAtt                                                                                                                                                      <  1:2423093/150‑1 (MQ=255)

 aGCTGCGAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGAtt                                                                                                                                                      <  1:163513/150‑1 (MQ=255)

     gCGAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGc                                                                                                                                                  <  2:2602480/150‑1 (MQ=255)

      cGAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGcc                                                                                                                                                 >  1:3375424/1‑150 (MQ=255)

       gAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCa                                                                                                                                                >  1:248599/1‑150 (MQ=255)

       gAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCa                                                                                                                                                >  2:813948/1‑150 (MQ=255)

       gAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCa                                                                                                                                                >  2:2931720/1‑150 (MQ=255)

        aaaGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCAt                                                                                                                                               <  1:832670/150‑1 (MQ=255)

         aaGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATg                                                                                                                                              <  2:3555134/150‑1 (MQ=255)

             gCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCa                                                                                                                                          <  2:4861575/150‑1 (MQ=255)

             gCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCa                                                                                                                                          >  2:1128603/1‑150 (MQ=255)

             gCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCa                                                                                                                                          >  2:4489636/1‑150 (MQ=255)

             gCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGAAATCGCCATGATCa                                                                                                                                          <  1:1569328/150‑1 (MQ=255)

                  gACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCGTGAGCATGGCGAAATCGCCATGATCATggtc                                                                                                                                     <  2:4781893/150‑3 (MQ=255)

                  gACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCGTGAGCATGGCGAAATCGCCATGATCATggtc                                                                                                                                     <  2:4781740/150‑3 (MQ=255)

                    ccGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCGTGAGCATGGCGAAATCGCCATGATCATggtcga                                                                                                                                   <  2:3174659/150‑5 (MQ=39)

                            ttGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGcggcgg                                                                                                                           <  1:3149979/150‑1 (MQ=255)

                            ttGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGcggcgg                                                                                                                           <  2:3225402/150‑1 (MQ=255)

                            ttGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGcggcgg                                                                                                                           >  2:2515342/1‑150 (MQ=255)

                              gAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGt                                                                                                                         >  1:4338547/1‑150 (MQ=255)

                                  tCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTAcgc                                                                                                                     <  1:4647655/150‑1 (MQ=255)

                                    tttGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTAcgcgc                                                                                                                   <  1:558909/150‑1 (MQ=255)

                                     ttGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGcggcgg                                                                                                                           <  2:1875089/141‑1 (MQ=255)

                                     ttGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGcggcgg                                                                                                                           >  1:1875089/1‑141 (MQ=255)

                                      tGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTAcgcgcgc                                                                                                                 <  1:200580/150‑1 (MQ=255)

                                         ccAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCa                                                                                                              <  1:1131055/150‑1 (MQ=255)

                                               ccTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCg                                                                                                        <  1:2187138/150‑1 (MQ=255)

                                                cTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGa                                                                                                       <  2:4861954/150‑1 (MQ=255)

                                                cTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGa                                                                                                       <  2:3856636/150‑1 (MQ=255)

                                                 ttGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAg                                                                                                      <  1:4796253/150‑1 (MQ=255)

                                                  tGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAgg                                                                                                     <  1:789749/150‑1 (MQ=255)

                                                     gcgagcgGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGc                                                                                                  >  2:3716100/1‑150 (MQ=255)

                                                      cgagcgGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCt                                                                                                 <  1:3748015/150‑1 (MQ=255)

                                                           gggAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATAc                                                                                            >  2:585922/1‑150 (MQ=255)

                                                              aaaTCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACccg                                                                                         >  1:1324013/1‑150 (MQ=255)

                                                               aaTCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACccgc                                                                                        >  2:2580517/1‑150 (MQ=255)

                                                                   tcGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTa                                                                                    >  2:1286353/1‑150 (MQ=255)

                                                                   tcGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTa                                                                                    >  2:1333725/1‑150 (MQ=255)

                                                                     ggACATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTAtc                                                                                  >  2:3027850/1‑150 (MQ=255)

                                                                     ggAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTAtc                                                                                  <  1:2580517/150‑1 (MQ=255)

                                                                     ggAAAGTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTAtc                                                                                  <  2:3584754/150‑1 (MQ=255)

                                                                          tttCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGa                                                                             <  1:3571047/150‑1 (MQ=255)

                                                                          tttCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGa                                                                             <  2:5104019/150‑1 (MQ=255)

                                                                          tttCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCAGGTCCGAGGGGCTGATACCCGCCGTATCGTCGa                                                                             <  2:4300370/150‑1 (MQ=255)

                                                                               ttGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAAc                                                                        <  2:4483591/150‑1 (MQ=255)

                                                                                   tACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTCCGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAAtt                                                                    >  1:687603/1‑150 (MQ=255)

                                                                                   tACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAAtt                                                                    >  1:4886056/1‑150 (MQ=255)

                                                                                     cGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAAtttt                                                                  <  2:4802019/150‑1 (MQ=255)

                                                                                          gTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTc                                                             <  2:4308279/150‑1 (MQ=255)

                                                                                           tCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCg                                                            >  1:3620318/1‑150 (MQ=255)

                                                                                            cACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGt                                                           >  1:3099337/1‑150 (MQ=255)

                                                                                             aCCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGtt                                                          >  1:4771953/1‑150 (MQ=255)

                                                                                                  ccGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCa                                                     <  1:4557876/150‑1 (MQ=255)

                                                                                                      ccccGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGtt                                                 <  2:422798/150‑1 (MQ=255)

                                                                                                       cccGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTc                                                >  1:5197993/1‑150 (MQ=255)

                                                                                                        ccGGAATCTGCATCAGCTGCAGGTAGTCGACCATGCTCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGCACAATTTTCGGTCGTTGAGCAGGTTCa                                               <  2:1706999/150‑1 (MQ=255)

                                                                                                        ccGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCa                                               >  2:936881/1‑150 (MQ=255)

                                                                                                        ccGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCa                                               >  1:1422290/1‑150 (MQ=255)

                                                                                                         cGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCAc                                              >  1:5338960/1‑150 (MQ=255)

                                                                                                         cGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAAATTTCGGTCGTTGAGCAGGTTCAc                                              >  2:4545986/1‑150 (MQ=255)

                                                                                                          ggAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACt                                             >  1:5203689/1‑150 (MQ=255)

                                                                                                               cTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGa                                        <  1:3901502/150‑1 (MQ=255)

                                                                                                               cTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGa                                        <  2:1382489/150‑1 (MQ=255)

                                                                                                                tGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCTTATCGTCGATGGACAATTTTCGGTCGTGGAGCAGGTTCACTGCCGAg                                       >  1:192561/1‑150 (MQ=255)

                                                                                                                tGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAg                                       <  2:764853/150‑1 (MQ=255)

                                                                                                                tGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAg                                       >  2:4310525/1‑150 (MQ=255)

                                                                                                                tGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAg                                       >  1:4164306/1‑150 (MQ=255)

                                                                                                                tGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAg                                       >  1:1416871/1‑150 (MQ=255)

                                                                                                                tGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAg                                       >  2:889560/1‑150 (MQ=255)

                                                                                                                tGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAg                                       >  2:779129/1‑150 (MQ=255)

                                                                                                                 gCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAgg                                      <  1:2106300/150‑1 (MQ=255)

                                                                                                                  cATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGt                                     <  2:1656235/150‑1 (MQ=255)

                                                                                                                  cATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGt                                     <  2:3984010/150‑1 (MQ=255)

                                                                                                                    tCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCa                                   >  1:1072235/1‑150 (MQ=255)

                                                                                                                         tGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGc                                                                                                                                     <  2:577926/47‑1 (MQ=255)

                                                                                                                          gCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGcgcg                             <  1:2461482/150‑1 (MQ=255)

                                                                                                                            aGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAgg                                                   >  2:1799887/1‑126 (MQ=255)

                                                                                                                            aGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAgg                                                   <  1:1799887/126‑1 (MQ=255)

                                                                                                                            aGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGc                           <  2:3375424/150‑1 (MQ=255)

                                                                                                                              gTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCa                         <  2:2597838/150‑1 (MQ=255)

                                                                                                                              gTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCa                         >  2:1456239/1‑150 (MQ=255)

                                                                                                                                    gACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGGGCAGGTTCACTGCCg                                         <  2:1945221/128‑1 (MQ=255)

                                                                                                                                       cgtGAGCATGGCGAAATCGCCATGATCATGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGa                                        >  1:1183597/3‑126 (MQ=39)

                                                                                                                                       cATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGa                                                        <  2:1165154/110‑1 (MQ=255)

                                                                                                                                       cATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGTCAgtcgtcgtc                <  2:1509622/150‑1 (MQ=255)

                                                                                                                                       cATGATCATGGCGAGTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCAGTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGACCAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAgtcgtcgtc                >  2:4965989/1‑150 (MQ=255)

                                                                                                                                        aTGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAgtcgtcgtcg               >  2:2645627/1‑150 (MQ=255)

                                                                                                                                           aTCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTcc            >  1:4540836/1‑150 (MQ=255)

                                                                                                                                            tCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCa           >  2:4196112/1‑150 (MQ=255)

                                                                                                                                             cATGGCGAAATCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCTCGGCCAGTCGTCGTCGTCCAg          >  1:5326184/1‑150 (MQ=255)

                                                                                                                                             cATGGCGAAATCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAg          >  2:1588497/1‑150 (MQ=255)

                                                                                                                                              aTGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACccgc                                                                                        >  2:248935/1‑71 (MQ=255)

                                                                                                                                                ggCGATTTCGCCATGCTCATGGTCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGc                                                      >  1:900432/1‑103 (MQ=255)

                                                                                                                                                 gCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTgg      >  2:2805136/1‑150 (MQ=255)

                                                                                                                                                  cGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTGGc     <  1:2931720/150‑1 (MQ=255)

                                                                                                                                                  cGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTGGc     >  2:2130226/1‑150 (MQ=255)

                                                                                                                                                   gATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTGGcc    <  1:1840472/150‑1 (MQ=255)

                                                                                                                                                    aTTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTGGCCg   <  2:2236719/150‑1 (MQ=255)

                                                                                                                                                     tttCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTGGCCgg  >  1:3582668/1‑150 (MQ=255)

CAGCTGCGAAAGGGCAATGACCGGGCAGTTGAATTCTTTGGCCAGGGCCTTGAGCGAGCGGGAAATCTCGGAAATTTCGTTGGTACGGTTGTCACCGGCCGACCCCGGAATCTGCATCAGCTGCAGGTAGTCGACCATGATCATGGCGATTTCGCCATGCTCACGGGCCAGGCGGCGGGTACGCGCGCGCATTTCCGAGGGGCTGATACCCGCCGTATCGTCGATGAACAATTTTCGGTCGTTGAGCAGGTTCACTGCCGAGGTCAGGCGCGGCCAGTCGTCGTCGTCCAGCTGGCCGG  >  NC_002947/5543541‑5543839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`