BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,709,091	G→C	5.5%	L377L (CTG→CTC) ‡	PP_5009 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,709,091	0	G	C	5.5%	607.0 / 18.5	181	L377L (CTG→CTC) ‡	PP_5009	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (73/98); new base C (7/3); total (80/101)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 188 total aligned reads displayed.

TTCGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCAGT  >  NC_002947/5708942‑5709239

ttcGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                      >  2:1026058/1‑150 (MQ=255)

ttcGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                      >  2:5229211/1‑150 (MQ=255)

ttcGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                      >  1:4584001/1‑150 (MQ=255)

ttcGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                      >  2:3766278/1‑150 (MQ=255)

   gACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGc                                                                                                                                                   >  1:13467/1‑150 (MQ=255)

      cTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGAt                                                                                                                                                >  2:3472219/1‑150 (MQ=255)

       tGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATg                                                                                                                                               >  1:4211108/1‑150 (MQ=255)

        gCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGt                                                                                                                                              <  1:565337/150‑1 (MQ=255)

        gCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGt                                                                                                                                              <  2:4919618/150‑1 (MQ=255)

           ttCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGa                                                                                                                                           <  2:2718993/150‑1 (MQ=255)

            tCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                          <  1:4663171/150‑1 (MQ=255)

            tCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                          <  1:4739243/150‑1 (MQ=255)

            tCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                          <  2:2317577/150‑1 (MQ=255)

            tCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                          <  2:2155625/150‑1 (MQ=255)

            tCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                          >  1:5178865/1‑150 (MQ=255)

            tCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                          <  1:5349035/150‑1 (MQ=255)

                  gaacgaacTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATc                                                                                                                                    >  2:2474892/1‑150 (MQ=255)

                     cgaacTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCGCACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGt                                                                                                                                 <  1:815562/150‑1 (MQ=255)

                         cTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGAtt                                                                                                                             <  1:3472219/150‑1 (MQ=255)

                          tGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTa                                                                                                                            <  1:203737/150‑1 (MQ=255)

                             gcCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgc                                                                                                                         <  1:2930379/150‑1 (MQ=255)

                             gcCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgc                                                                                                                         <  2:5330007/150‑1 (MQ=255)

                               ccGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccg                                                                                                                       >  2:1610526/1‑150 (MQ=255)

                               ccGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccg                                                                                                                       >  2:3502222/1‑150 (MQ=255)

                               ccGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccg                                                                                                                       >  1:1204740/1‑150 (MQ=255)

                                  cTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctc                                                                                                                    <  1:4614968/150‑1 (MQ=255)

                                        gCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctcgccgct                                                                                                              >  2:3935694/1‑150 (MQ=255)

                                         cccACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCtt                                                                                                             <  1:2989599/150‑1 (MQ=255)

                                           cACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGc                                                                                                           >  2:2964491/1‑150 (MQ=255)

                                            aCCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCg                                                                                                          >  1:857680/1‑150 (MQ=255)

                                            aCCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCg                                                                                                          >  1:4176006/1‑150 (MQ=255)

                                            aCCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCg                                                                                                          >  1:4176380/1‑150 (MQ=255)

                                             ccGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGt                                                                                                         <  1:695545/150‑1 (MQ=255)

                                             ccGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGt                                                                                                         <  1:1553432/150‑1 (MQ=255)

                                              cgcgATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTg                                                                                                        <  1:1020546/150‑1 (MQ=255)

                                               gcgATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTga                                                                                                       <  1:1009466/150‑1 (MQ=255)

                                                    ggCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGAccc                                                                                                  <  1:3428089/150‑1 (MQ=255)

                                                      ctgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTg                                                                                                >  1:278254/1‑150 (MQ=255)

                                                        gctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATcc                                                                                                                                   <  2:4298806/113‑1 (MQ=255)

                                                        gctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCa                                                                                              <  1:2641612/150‑1 (MQ=255)

                                                        gctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCa                                                                                              <  1:4760576/150‑1 (MQ=255)

                                                          tCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTgag                                                                                                      <  2:1765493/140‑1 (MQ=255)

                                                          tCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTgag                                                                                                      >  1:1765493/1‑140 (MQ=255)

                                                             aTGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCa                                                                                         <  2:1003735/150‑1 (MQ=255)

                                                             aTGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCa                                                                                         <  1:3283434/150‑1 (MQ=255)

                                                                ccAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCt                                                                                      >  2:4724652/1‑150 (MQ=255)

                                                                 cAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTa                                                                                     >  1:1103688/1‑150 (MQ=255)

                                                                 cAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTa                                                                                     >  1:3383633/1‑150 (MQ=255)

                                                                   gcgcTGAGTACCGCTTCGCCTATCACACGGCTCTGCTCGTTCTGGCGTCGAACGTAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGTTATCCGTGATTACTCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATc                                                                                   <  2:1174589/150‑1 (MQ=255)

                                                                   gcgcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATc                                                                                   <  2:3028116/150‑1 (MQ=255)

                                                                    cgcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATcc                                                                                  <  1:210358/150‑1 (MQ=255)

                                                                    cgcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATcc                                                                                  <  2:1068164/150‑1 (MQ=255)

                                                                     gcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCa                                                                                 <  1:3301728/150‑1 (MQ=255)

                                                                     gcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCa                                                                                 <  2:611543/150‑1 (MQ=255)

                                                                     gcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCa                                                                                 <  1:2119118/150‑1 (MQ=255)

                                                                     gcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCGTGCGTGAGACCCTGCAGACCAGCTATCCa                                                                                 <  2:3337205/150‑1 (MQ=255)

                                                                      cTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCtt                                                                                                             >  1:272406/1‑121 (MQ=255)

                                                                      cTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCtt                                                                                                             <  2:272406/121‑1 (MQ=255)

                                                                      cTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAg                                                                                >  2:142478/1‑150 (MQ=255)

                                                                            aCCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAgg                                                                          <  2:2436796/150‑1 (MQ=255)

                                                                             ccGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGc                                                                         <  2:5410990/150‑1 (MQ=255)

                                                                              cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCa                                                                        <  2:4806053/150‑1 (MQ=255)

                                                                              cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCa                                                                        <  1:3778546/150‑1 (MQ=255)

                                                                              cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCa                                                                        <  2:947557/150‑1 (MQ=255)

                                                                              cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCa                                                                        <  2:2013845/150‑1 (MQ=255)

                                                                                      cTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGa                                                                <  1:2184368/150‑1 (MQ=255)

                                                                                            cacGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGaa                                                          <  1:142478/150‑1 (MQ=255)

                                                                                                gCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                      <  2:2850411/150‑1 (MQ=255)

                                                                                                   cTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAtgtg                                                   <  2:4176006/150‑1 (MQ=255)

                                                                                                          aTCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATcc                                            <  2:4616884/150‑1 (MQ=255)

                                                                                                             tGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCa                                         >  1:3644938/1‑150 (MQ=255)

                                                                                                             tGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCa                                         >  2:5259303/1‑150 (MQ=255)

                                                                                                              ggcggcGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCAt                                        <  2:1271112/150‑1 (MQ=255)

                                                                                                                   cgAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTccc                                   <  1:5259303/150‑1 (MQ=255)

                                                                                                                    gAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCa                                  <  1:321117/150‑1 (MQ=255)

                                                                                                                     aaCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                                 <  1:1881292/150‑1 (MQ=255)

                                                                                                                     aaCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                                 >  1:3186742/1‑150 (MQ=255)

                                                                                                                     aaCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                                 <  1:2474892/150‑1 (MQ=255)

                                                                                                                     aaCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                                 <  1:1638143/150‑1 (MQ=255)

                                                                                                                     aaCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                                 <  1:2305397/150‑1 (MQ=255)

                                                                                                                      aCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAg                                >  2:353997/1‑150 (MQ=255)

                                                                                                                              cggtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCGatcatc                        <  2:4177068/150‑1 (MQ=255)

                                                                                                                               ggtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCg                       >  2:2173843/1‑150 (MQ=255)

                                                                                                                                   gAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCcat                            >  1:1690025/1‑141 (MQ=255)

                                                                                                                                   gAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCcat                            <  2:1690025/141‑1 (MQ=255)

                                                                                                                                     aGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGTACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCg                 <  1:3697276/150‑1 (MQ=255)

                                                                                                                                        cttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                      <  2:2110580/105‑1 (MQ=255)

                                                                                                                                         ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                                 <  2:5175336/126‑1 (MQ=255)

                                                                                                                                         ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                                 >  1:5175336/5‑130 (MQ=255)

                                                                                                                                         ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGcac             >  2:3417383/5‑150 (MQ=255)

                                                                                                                                         ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGcac             >  2:3182358/5‑150 (MQ=255)

                                                                                                                                          tGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGcaca            >  2:4614806/1‑150 (MQ=255)

                                                                                                                                               aTCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAgg       >  2:3944856/1‑150 (MQ=255)

                                                                                                                                               aTCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAgg       >  2:5375731/1‑150 (MQ=255)

                                                                                                                                                  gCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGcacac           <  2:160747/143‑1 (MQ=255)

                                                                                                                                                  gCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCa    <  2:90230/150‑1 (MQ=255)

                                                                                                                                                   cTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCAg   <  2:664313/150‑1 (MQ=255)

                                                                                                                                                    tGGGCGATGTCGAAGATATCCGTGTTTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCAGt  <  2:1735235/150‑1 (MQ=255)

                                                                                                                                                    tGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCAGt  <  2:3644938/150‑1 (MQ=255)

                                                                                                                                                    tGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCAGt  <  1:2964491/150‑1 (MQ=255)

TTCGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCAGT  >  NC_002947/5708942‑5709239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`