BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,714,209	C→T	5.5%	D100D (GAC→GAT) ‡	PP_5012 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,714,209	0	C	T	5.5%	626.3 / 16.9	199	D100D (GAC→GAT) ‡	PP_5012	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (98/90); new base T (6/5); total (104/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 199 total aligned reads displayed.

CCGATGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTG  >  NC_002947/5714061‑5714356

ccGATGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACa                                                                                                                                                    <  1:2911915/150‑1 (MQ=255)

  gATGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAgc                                                                                                                                                  >  2:1834702/1‑150 (MQ=255)

    tGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAgcgc                                                                                                                                                <  2:4731112/150‑1 (MQ=255)

     gAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGcc                                                                                                                                               <  1:4666304/150‑1 (MQ=255)

          gggCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCTCATCTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGtgca                                                                                                                                          <  2:2869446/150‑4 (MQ=255)

           ggCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAgcg                                                                                                                                                 <  2:1200632/142‑1 (MQ=255)

           ggCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTg                                                                                                                                         >  1:3393630/1‑150 (MQ=255)

           ggCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTg                                                                                                                                         >  1:3701463/1‑150 (MQ=255)

             ccTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCt                                                                                                                                       <  1:1600883/150‑1 (MQ=255)

              cTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTc                                                                                                                                      <  1:541003/150‑1 (MQ=255)

                 aTGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGAc                                                                                                                                   <  2:3086815/150‑1 (MQ=255)

                 aTGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGAc                                                                                                                                   <  1:1733854/150‑1 (MQ=255)

                   gCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCt                                                                                                                                 <  2:1630902/150‑1 (MQ=255)

                    cTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTg                                                                                                                                >  2:4297324/1‑150 (MQ=255)

                      cgccgcCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTgcg                                                                                                                              <  2:1531100/150‑3 (MQ=38)

                        ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                            >  1:1629188/1‑150 (MQ=255)

                        ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                            <  2:3663897/150‑1 (MQ=255)

                        ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                            <  2:1282160/150‑1 (MQ=255)

                        ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCACAGGTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTgcggg                                                                                                                            <  1:1328374/150‑5 (MQ=37)

                          gccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgt                                                                                                                          <  2:2512855/150‑1 (MQ=255)

                           cccATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                         <  1:1084631/150‑1 (MQ=255)

                            ccATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtgt                                                                                                                        <  1:1686661/150‑1 (MQ=255)

                             cATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGtgtg                                                                                                                       >  2:2192078/1‑150 (MQ=255)

                                  gCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCa                                                                                                                  <  1:2274520/150‑1 (MQ=255)

                                    aGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                         >  2:2163585/1‑141 (MQ=255)

                                    aGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                         <  1:2163585/141‑1 (MQ=255)

                                    aGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAgg                                                                                                                >  2:2144327/1‑150 (MQ=255)

                                      ggCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGAc                                                                                                              <  1:5209776/150‑1 (MQ=255)

                                       gCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGAcc                                                                                                             >  2:4412689/1‑150 (MQ=255)

                                           ggTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAACCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGa                                                                                                         >  2:3003291/1‑150 (MQ=255)

                                               gACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTg                                                                                                     >  1:348934/1‑150 (MQ=255)

                                               gACTGCCGCCTGCGCGCCCCCGCCGATAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTg                                                                                                     <  2:2099776/150‑1 (MQ=255)

                                                aCTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTgg                                                                                                    >  1:5354769/1‑150 (MQ=255)

                                                    ccgccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTg                                                                                                <  2:2480537/150‑1 (MQ=255)

                                                      gccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTggg                                                                                              >  1:4534964/1‑150 (MQ=255)

                                                       cccgcgcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                             <  1:156386/147‑1 (MQ=255)

                                                       ccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                             >  1:3622739/1‑150 (MQ=255)

                                                       ccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                             <  2:5138926/150‑1 (MQ=255)

                                                          gcgcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCa                                                                                          >  2:1515038/1‑150 (MQ=255)

                                                                  ccgccgGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGc                                                                                  >  2:1914367/1‑150 (MQ=255)

                                                                           gCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGGGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGc                                                                         <  2:171112/150‑1 (MQ=255)

                                                                            ccTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCt                                                                        >  1:1416637/1‑150 (MQ=255)

                                                                             cTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCtt                                                                       >  1:981299/1‑150 (MQ=255)

                                                                              tGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTg                                                                      >  2:60849/1‑150 (MQ=255)

                                                                              tGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTg                                                                      >  1:1656610/1‑150 (MQ=255)

                                                                               ggggcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACTGCGCCGTACTGCTCGTCGTGTTCGGTGTGCTGCAGGACGTGGAGCTGGAGTGGGAGCTCGAGCTGCAACGCTGGCTTgg                                                                     >  2:2579959/4‑150 (MQ=255)

                                                                               ggCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTgg                                                                     >  2:427814/1‑150 (MQ=255)

                                                                                gcgcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGGTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGc                                                                    <  2:4188843/150‑1 (MQ=255)

                                                                                  gcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGccc                                                                  <  1:2812547/150‑1 (MQ=255)

                                                                                    tcttgtccTTGACCAAGGCCAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCgg                                                                >  1:2104822/7‑150 (MQ=255)

                                                                                     aCTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGt                                                               >  1:781583/1‑150 (MQ=255)

                                                                                      cTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTc                                                              <  1:5300296/150‑1 (MQ=255)

                                                                                           cTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCAc                                                         >  2:3217799/1‑150 (MQ=255)

                                                                                             tGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTTGCCCGGTCTCCACgg                                                       <  2:4828917/150‑1 (MQ=255)

                                                                                             tGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACgg                                                       <  1:609784/150‑1 (MQ=255)

                                                                                                tCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCtt                                                                       >  1:4348705/1‑131 (MQ=255)

                                                                                                tCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCtt                                                                       <  2:4348705/131‑1 (MQ=255)

                                                                                                 cAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAAt                                                   >  2:5007619/1‑150 (MQ=255)

                                                                                                  aaGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATg                                                  <  1:1463487/150‑1 (MQ=255)

                                                                                                    ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCt                                                >  1:4408316/1‑150 (MQ=255)

                                                                                                    ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCt                                                >  2:3755216/1‑150 (MQ=255)

                                                                                                    ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCGCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGGCTGGGAGCCCGAGCGGCAACGCTGGCTTGGCCCGGTCGCCACGGCCATGCt                                                >  1:5035435/1‑150 (MQ=255)

                                                                                                     gACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTg                                               >  2:2427908/1‑150 (MQ=255)

                                                                                                        aaGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggcc                                            >  1:3184410/1‑150 (MQ=255)

                                                                                                        aaGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggcc                                            >  2:450550/1‑150 (MQ=255)

                                                                                                         aGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccg                                           <  2:1426622/150‑1 (MQ=255)

                                                                                                          gACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccgg                                          >  2:920327/1‑150 (MQ=255)

                                                                                                           aCCGCTGTGCTGCACAGCCCGCAGGTCGAGGTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggc                                         <  2:714134/150‑1 (MQ=255)

                                                                                                           aCCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggc                                         <  1:3122980/150‑1 (MQ=255)

                                                                                                           aCCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTAGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCAGAAACGCTGGCTAGGCCCGGTCGCCACGGCAATGCTggccggc                                         >  2:2318339/1‑150 (MQ=255)

                                                                                                            ccGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggcc                                        <  2:5279809/150‑1 (MQ=255)

                                                                                                            aggtcgagctgcACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggcc                                        <  1:2687564/143‑1 (MQ=255)

                                                                                                             cGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCAGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCAGGCCGGCCa                                       >  1:1844498/1‑150 (MQ=255)

                                                                                                                tgtgCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCatat                                    <  1:5418453/150‑1 (MQ=255)

                                                                                                                tgtgCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCatat                                    >  1:4339685/1‑150 (MQ=255)

                                                                                                                  tgctgcACAGCCCGCAGGTCGTGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTTCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATcc                                  >  1:3032407/1‑150 (MQ=255)

                                                                                                                      gcACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCt                              <  1:1542730/150‑1 (MQ=255)

                                                                                                                         caGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcg                           >  2:3523818/1‑150 (MQ=255)

                                                                                                                          aGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcgc                          >  1:1135834/1‑150 (MQ=255)

                                                                                                                              cGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctc                      >  2:2033235/1‑150 (MQ=255)

                                                                                                                                 aGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcgct                   <  2:5148835/150‑1 (MQ=255)

                                                                                                                                 aGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcgct                   <  1:4297067/150‑1 (MQ=255)

                                                                                                                                  ggTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTg                  >  1:1485016/1‑150 (MQ=255)

                                                                                                                                  ggTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTg                  >  1:229466/1‑150 (MQ=38)

                                                                                                                                   gTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCT‑GACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGa                >  2:1614280/1‑150 (MQ=255)

                                                                                                                                   gTCGAGCAGTACGGCGCTGTCGCCGTGCTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTgg                 >  1:2467818/1‑150 (MQ=39)

                                                                                                                                   gTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTGCGGGCTGTGCAGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTgg                 >  1:4153817/1‑150 (MQ=25)

                                                                                                                                      gAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGcc                                                                   >  1:388226/1‑97 (MQ=255)

                                                                                                                                      gAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGcc                                                                   <  2:388226/97‑1 (MQ=255)

                                                                                                                                        gCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCg            >  2:3379932/1‑150 (MQ=255)

                                                                                                                                         cTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGc           <  2:2545592/150‑1 (MQ=255)

                                                                                                                                          tGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTTGc                                                                    <  1:1088034/92‑1 (MQ=255)

                                                                                                                                          tGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTTGc                                                                    >  2:1088034/1‑92 (MQ=255)

                                                                                                                                             aCGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAg       >  2:3911976/1‑150 (MQ=255)

                                                                                                                                              cGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAgg      >  2:848709/1‑150 (MQ=255)

                                                                                                                                               ggCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGc     <  1:2021288/150‑1 (MQ=255)

                                                                                                                                                 cGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCt   <  2:4137387/150‑1 (MQ=255)

                                                                                                                                                 cGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCt   <  1:5157454/150‑1 (MQ=255)

                                                                                                                                                  gACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTg  <  1:1753206/150‑1 (MQ=255)

                                                                                                                                                  gACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTg  <  1:533489/150‑1 (MQ=255)

CCGATGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTG  >  NC_002947/5714061‑5714356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`