BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,714,211	G→T	5.7%	S101I (AGC→ATC) ‡	PP_5012 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,714,211	0	G	T	5.7%	638.2 / 20.3	192	S101I (AGC→ATC) ‡	PP_5012	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (95/86); new base T (6/5); total (101/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

Only 100 of 196 total aligned reads displayed.

GATGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTG  >  NC_002947/5714063‑5714356

gATGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAgc                                                                                                                                                  >  2:1834702/1‑150 (MQ=255)

  tGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAgcgc                                                                                                                                                <  2:4731112/150‑1 (MQ=255)

        gggCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCTCATCTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGtgca                                                                                                                                          <  2:2869446/150‑4 (MQ=255)

         ggCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAgcg                                                                                                                                                 <  2:1200632/142‑1 (MQ=255)

          gCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGc                                                                                                                                        <  1:4483190/150‑1 (MQ=255)

           ccTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCt                                                                                                                                       <  1:1600883/150‑1 (MQ=255)

              gATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGa                                                                                                                                    <  1:363960/150‑1 (MQ=255)

               aTGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGAc                                                                                                                                   <  1:1733854/150‑1 (MQ=255)

               aTGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGAc                                                                                                                                   <  2:3086815/150‑1 (MQ=255)

                 gCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCt                                                                                                                                 <  2:1630902/150‑1 (MQ=255)

                  cTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTg                                                                                                                                >  2:4297324/1‑150 (MQ=255)

                   tCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGt                                                                                                                               >  1:3407623/1‑150 (MQ=255)

                    cgccgcCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTgcg                                                                                                                              <  2:1531100/150‑3 (MQ=38)

                      ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                            <  2:1282160/150‑1 (MQ=255)

                      ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                            >  1:1629188/1‑150 (MQ=255)

                      ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                            <  2:3663897/150‑1 (MQ=255)

                      ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCACAGGTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTgcggg                                                                                                                            <  1:1328374/150‑5 (MQ=37)

                        gccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgt                                                                                                                          <  2:2512855/150‑1 (MQ=255)

                         cccATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                         <  1:1084631/150‑1 (MQ=255)

                          ccATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtgt                                                                                                                        <  1:1686661/150‑1 (MQ=255)

                           cATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGtgtg                                                                                                                       >  2:2192078/1‑150 (MQ=255)

                                gCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCa                                                                                                                  <  1:2274520/150‑1 (MQ=255)

                                gCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCa                                                                                                                  <  1:661798/150‑1 (MQ=255)

                                  aGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                         >  2:2163585/1‑141 (MQ=255)

                                  aGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                         <  1:2163585/141‑1 (MQ=255)

                                  aGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAgg                                                                                                                >  2:2144327/1‑150 (MQ=255)

                                     gCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGAcc                                                                                                             >  2:4412689/1‑150 (MQ=255)

                                       gAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTg                                                                                                           <  1:4394097/150‑1 (MQ=255)

                                         ggTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAACCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGa                                                                                                         >  2:3003291/1‑150 (MQ=255)

                                            cGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCt                                                                                                      <  1:3221175/150‑1 (MQ=255)

                                             gACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTg                                                                                                     >  1:5409231/1‑150 (MQ=255)

                                             gACTGCCGCCTGCGCGCCCCCGCCGATAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTg                                                                                                     <  2:2099776/150‑1 (MQ=255)

                                                  ccgccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTg                                                                                                <  2:2480537/150‑1 (MQ=255)

                                                  ccgccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTg                                                                                                >  1:5279809/1‑150 (MQ=255)

                                                   cgccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTgg                                                                                               <  1:532408/150‑1 (MQ=255)

                                                    gccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTggg                                                                                              <  1:99529/150‑1 (MQ=255)

                                                     cccgcgcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                             <  1:156386/147‑1 (MQ=255)

                                                     ccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                             <  2:5138926/150‑1 (MQ=255)

                                                        gcgcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCa                                                                                          >  2:1515038/1‑150 (MQ=255)

                                                        gcgcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCa                                                                                          <  1:5436583/150‑1 (MQ=255)

                                                          gcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACg                                                                                        >  1:4572419/1‑150 (MQ=255)

                                                            gcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAg                                                                                      <  1:3570329/150‑1 (MQ=255)

                                                              ccccGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCt                                                                                    <  1:909191/150‑1 (MQ=255)

                                                                ccgccgGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGc                                                                                  >  2:1914367/1‑150 (MQ=255)

                                                                         gCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGGGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGc                                                                         <  2:171112/150‑1 (MQ=255)

                                                                          ccTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCt                                                                        >  1:1416637/1‑150 (MQ=255)

                                                                            tGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTg                                                                      >  2:60849/1‑150 (MQ=255)

                                                                            tGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTg                                                                      >  1:1656610/1‑150 (MQ=255)

                                                                             ggggcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACTGCGCCGTACTGCTCGTCGTGTTCGGTGTGCTGCAGGACGTGGAGCTGGAGTGGGAGCTCGAGCTGCAACGCTGGCTTgg                                                                     >  2:2579959/4‑150 (MQ=255)

                                                                             ggCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTgg                                                                     >  2:427814/1‑150 (MQ=255)

                                                                              gcgcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGGTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGc                                                                    <  2:4188843/150‑1 (MQ=255)

                                                                                gcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGccc                                                                  <  1:2812547/150‑1 (MQ=255)

                                                                                 cAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCg                                                                 <  1:388615/150‑1 (MQ=255)

                                                                                  tcttgtccTTGACCAAGGCCAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCgg                                                                >  1:2104822/7‑150 (MQ=255)

                                                                                   aCTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGt                                                               >  1:3875577/1‑150 (MQ=255)

                                                                                           tGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTTGCCCGGTCTCCACgg                                                       <  2:4828917/150‑1 (MQ=255)

                                                                                              tCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCtt                                                                       <  2:4348705/131‑1 (MQ=255)

                                                                                               cAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAAt                                                   >  2:5007619/1‑150 (MQ=255)

                                                                                               cAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAAt                                                   >  1:3137233/1‑150 (MQ=255)

                                                                                                aaGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATg                                                  <  1:1463487/150‑1 (MQ=255)

                                                                                                  ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCt                                                >  2:3755216/1‑150 (MQ=255)

                                                                                                  ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGACGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGACGCCACGGCaaagat                                                >  2:3097482/1‑146 (MQ=255)

                                                                                                   gACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTg                                               >  2:2427908/1‑150 (MQ=255)

                                                                                                      aaGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggcc                                            >  2:450550/1‑150 (MQ=255)

                                                                                                       aGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccg                                           <  2:1426622/150‑1 (MQ=255)

                                                                                                        gACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccgg                                          >  2:920327/1‑150 (MQ=255)

                                                                                                         aCCGCTGTGCTGCACAGCCCGCAGGTCGAGGTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggc                                         <  2:714134/150‑1 (MQ=255)

                                                                                                         aCCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggc                                         <  1:3122980/150‑1 (MQ=255)

                                                                                                         aCCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTAGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCAGAAACGCTGGCTAGGCCCGGTCGCCACGGCAATGCTggccggc                                         >  2:2318339/1‑150 (MQ=255)

                                                                                                          ccGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggcc                                        <  2:5279809/150‑1 (MQ=255)

                                                                                                          aggtcgagctgcACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggcc                                        <  1:2687564/143‑1 (MQ=255)

                                                                                                           cGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCAGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCAGGCCGGCCa                                       >  1:1844498/1‑150 (MQ=255)

                                                                                                              tgtgCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCatat                                    <  1:5418197/150‑1 (MQ=255)

                                                                                                                tgctgcACAGCCCGCAGGTCGTGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTTCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATcc                                  >  1:3032407/1‑150 (MQ=255)

                                                                                                                    gcACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCt                              <  1:1542730/150‑1 (MQ=255)

                                                                                                                       caGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcg                           >  2:3523818/1‑150 (MQ=255)

                                                                                                                        aGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcgc                          >  1:1135834/1‑150 (MQ=255)

                                                                                                                          cccGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcgcgc                        >  1:4170308/1‑150 (MQ=255)

                                                                                                                            cGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctc                      >  2:2033235/1‑150 (MQ=255)

                                                                                                                             gCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcg                     >  1:5148835/1‑150 (MQ=255)

                                                                                                                             gCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcg                     >  1:5020169/1‑150 (MQ=255)

                                                                                                                               aGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcgct                   <  2:5148835/150‑1 (MQ=255)

                                                                                                                               aGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcgct                   <  1:4297324/150‑1 (MQ=255)

                                                                                                                                ggTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTg                  >  1:1485016/1‑150 (MQ=255)

                                                                                                                                ggTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTg                  >  1:229466/1‑150 (MQ=38)

                                                                                                                                 gTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCT‑GACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGa                >  2:1614280/1‑150 (MQ=255)

                                                                                                                                 gTCGAGCAGTACGGCGCTGTCGCCGTGCTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTgg                 >  1:2467818/1‑150 (MQ=39)

                                                                                                                                    gAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGcc                                                                   <  2:388226/97‑1 (MQ=255)

                                                                                                                                       cTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGc           <  2:2545592/150‑1 (MQ=255)

                                                                                                                                        tGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTTGc                                                                    <  1:1088034/92‑1 (MQ=255)

                                                                                                                                        tGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTTGc                                                                    >  2:1088034/1‑92 (MQ=255)

                                                                                                                                         gCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCa         >  2:3272353/1‑150 (MQ=255)

                                                                                                                                          cACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCaa        >  1:5176115/1‑150 (MQ=255)

                                                                                                                                           aCGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAg       >  2:3911976/1‑150 (MQ=255)

                                                                                                                                            cGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAgg      >  1:4348620/1‑150 (MQ=255)

                                                                                                                                            cGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAgg      >  2:848709/1‑150 (MQ=255)

                                                                                                                                             ggCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGc     <  1:2021288/150‑1 (MQ=255)

                                                                                                                                               cGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCt   <  2:4137387/150‑1 (MQ=255)

                                                                                                                                                gACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTg  <  1:1753206/150‑1 (MQ=255)

                                                                                                                                                gACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCGGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTg  <  1:533763/150‑1 (MQ=255)

GATGAAGAGGGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTG  >  NC_002947/5714063‑5714356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`