BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,714,220	T→A	5.1%	L104Q (CTG→CAG)	PP_5012 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,714,220	0	T	A	5.1%	679.9 / 16.1	201	L104Q (CTG→CAG)	PP_5012	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (99/91); new base A (3/7); total (102/99)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 204 total aligned reads displayed.

GGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTGGCACGTTTCAGCC  >  NC_002947/5714072‑5714369

ggCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTg                                                                                                                                                      >  2:2274520/1‑150 (MQ=255)

 gCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGc                                                                                                                                                     <  1:4483190/150‑1 (MQ=255)

  ccTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCt                                                                                                                                                    <  2:2180613/150‑1 (MQ=255)

  ccTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCt                                                                                                                                                    <  2:1854563/150‑1 (MQ=255)

   cTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTc                                                                                                                                                   <  1:541003/150‑1 (MQ=255)

      aTGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGAc                                                                                                                                                >  2:2812547/1‑150 (MQ=255)

      aTGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGAc                                                                                                                                                <  1:1733859/150‑1 (MQ=255)

      aTGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGAc                                                                                                                                                <  1:1733714/150‑1 (MQ=255)

        gCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCt                                                                                                                                              <  2:1630902/150‑1 (MQ=255)

         cTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTg                                                                                                                                             >  2:4297324/1‑150 (MQ=255)

          tCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGt                                                                                                                                            >  1:3407623/1‑150 (MQ=255)

           cgccgcCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTgcg                                                                                                                                           <  2:1531096/150‑3 (MQ=38)

             ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                                         >  1:1629007/1‑150 (MQ=255)

             ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCg                                                                                                                                         <  2:1282160/150‑1 (MQ=255)

             ccgccCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCACAGGTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTgcggg                                                                                                                                         <  1:1328374/150‑5 (MQ=37)

                cccATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                                      <  1:1084631/150‑1 (MQ=255)

                 ccATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtgt                                                                                                                                     >  2:2575419/1‑150 (MQ=255)

                   aTTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGtgc                                                                                                                                   >  2:2021288/1‑150 (MQ=255)

                    ttGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGtgct                                                                                                                                  >  2:4956217/1‑150 (MQ=255)

                       gCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCa                                                                                                                               <  1:2274520/150‑1 (MQ=255)

                       gCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCa                                                                                                                               <  2:2469890/150‑1 (MQ=255)

                        cAGGGCCAGCTCGACGGCCGCCTGCGCGCCCCCGCCGCTACCCTGGCGCAACTGGCCATGGTCAAGGACAAGACCGCTGTGCCGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACCGCTCGACCTGTTCGGTGTCCTGCAg                                                                                                                              <  2:1568571/150‑1 (MQ=255)

                         aGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGgtg                                                                                                                                      <  1:2163585/141‑1 (MQ=255)

                           ggCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGAc                                                                                                                           <  1:5209776/150‑1 (MQ=255)

                            gCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGAcc                                                                                                                          >  2:4412689/1‑150 (MQ=255)

                              gAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTg                                                                                                                        <  1:4394097/150‑1 (MQ=255)

                                ggTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGAGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGa                                                                                                                      <  2:625552/150‑1 (MQ=255)

                                  tCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGc                                                                                                                    >  1:1805500/1‑150 (MQ=255)

                                   cGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCt                                                                                                                   <  1:3221175/150‑1 (MQ=255)

                                    gACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTg                                                                                                                  >  1:348934/1‑150 (MQ=255)

                                     aCTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTgg                                                                                                                 >  1:5354769/1‑150 (MQ=255)

                                         ccgccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCCGCAGGACCTGGAGCTGGACTg                                                                                                             >  2:3080648/1‑150 (MQ=255)

                                           gccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTggg                                                                                                           <  1:99529/150‑1 (MQ=255)

                                            cccgcgcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                                          <  1:156386/147‑1 (MQ=255)

                                            ccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                                          >  1:3622739/1‑150 (MQ=255)

                                            ccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                                          <  2:2049462/150‑1 (MQ=255)

                                            ccTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGa                                                                                                          <  2:5139016/150‑1 (MQ=255)

                                                 gcgcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACg                                                                                                     >  1:4572419/1‑150 (MQ=255)

                                                   gcCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAg                                                                                                   >  2:3578382/1‑150 (MQ=255)

                                                                gCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGGGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGc                                                                                      <  2:171112/150‑1 (MQ=255)

                                                                 ccTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCt                                                                                     >  1:1119083/1‑150 (MQ=255)

                                                                 ccTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCt                                                                                     >  1:1416637/1‑150 (MQ=255)

                                                                   tGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTg                                                                                   <  2:5297618/150‑1 (MQ=255)

                                                                    ggCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTgg                                                                                  >  2:427814/1‑150 (MQ=255)

                                                                     gcgcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGGTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGc                                                                                 <  2:4188843/150‑1 (MQ=255)

                                                                       gcAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGccc                                                                               <  1:2812547/150‑1 (MQ=255)

                                                                        cAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCg                                                                              <  1:388615/150‑1 (MQ=255)

                                                                         tcttgtccTTGACCAAGGCCAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCgg                                                                             >  1:2104822/7‑150 (MQ=255)

                                                                          aCTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGt                                                                            >  1:781583/1‑150 (MQ=255)

                                                                          aCTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGt                                                                            >  1:3875577/1‑150 (MQ=255)

                                                                           ttgtccTTGACCAAGGCCAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTc                                                                           >  2:2500054/5‑150 (MQ=255)

                                                                           cTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTc                                                                           <  1:5300296/150‑1 (MQ=255)

                                                                           cTGGCCGTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTc                                                                           <  2:3875577/150‑1 (MQ=255)

                                                                            tGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCg                                                                          >  2:2532700/1‑150 (MQ=255)

                                                                                cTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCAc                                                                      >  1:1639934/1‑150 (MQ=255)

                                                                                  tGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACgg                                                                    <  1:609784/150‑1 (MQ=255)

                                                                                     tCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCtt                                                                                    <  2:4348705/131‑1 (MQ=255)

                                                                                     tCAAGGACAAGACCGCTGTGCAGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGAGCCGTACTGCACGACCTGGTCGGTGTGCTGCAGGACCTGGAGGTGGACTGGGAGAACGAGCTGCAACGAAGGCTTGGCCCGGTCGCCACGGCaa                                                                 >  2:505647/1‑150 (MQ=255)

                                                                                      cAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAAt                                                                >  1:3137233/1‑150 (MQ=255)

                                                                                       aaGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATg                                                               <  1:1463487/150‑1 (MQ=255)

                                                                                         ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCt                                                             <  1:1681471/150‑1 (MQ=255)

                                                                                         ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCGCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGGCTGGGAGCCCGAGCGGCAACGCTGGCTTGGCCCGGTCGCCACGGCCATGCt                                                             >  1:5035435/1‑150 (MQ=255)

                                                                                         ggACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGACGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGACGCCACGGCaaagat                                                             >  2:3097482/1‑146 (MQ=255)

                                                                                          gACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTg                                                            >  2:2101269/1‑150 (MQ=255)

                                                                                          gACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTg                                                            <  2:3887977/150‑1 (MQ=255)

                                                                                             aaGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggcc                                                         >  2:450550/1‑150 (MQ=255)

                                                                                             aaGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggcc                                                         >  2:757165/1‑150 (MQ=255)

                                                                                              aGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccg                                                        <  2:1426622/150‑1 (MQ=255)

                                                                                               gACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccgg                                                       >  2:3968466/1‑150 (MQ=255)

                                                                                                 aggtcgagctgcACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTggccggcc                                                     <  1:2687564/143‑1 (MQ=255)

                                                                                                     tgtgCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCatat                                                 >  2:1101976/1‑150 (MQ=255)

                                                                                                     tgtgCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCatat                                                 <  1:5418453/150‑1 (MQ=255)

                                                                                                       tgctgcACAGCCCGCAGGTCGTGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTTCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATcc                                               >  1:3032407/1‑150 (MQ=255)

                                                                                                           gcACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCt                                           <  1:1542730/150‑1 (MQ=255)

                                                                                                           gcACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCt                                           <  2:854231/150‑1 (MQ=255)

                                                                                                                 cccGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcgcgc                                     >  1:4170308/1‑150 (MQ=255)

                                                                                                                 cccGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcgcgc                                     >  2:3679134/1‑150 (MQ=255)

                                                                                                                 cccGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTgcgcgc                                     >  2:2424505/1‑150 (MQ=255)

                                                                                                                   cGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctc                                   >  2:4771161/1‑150 (MQ=255)

                                                                                                                      aGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcgct                                <  2:5247967/150‑1 (MQ=255)

                                                                                                                      aGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGcgctcgct                                <  1:4297324/150‑1 (MQ=255)

                                                                                                                       ggTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTg                               >  1:1485016/1‑150 (MQ=255)

                                                                                                                       ggTCGAGCAGTACGGCGCTGTCGCCGTGCAGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTg                               >  1:229466/1‑150 (MQ=38)

                                                                                                                        gTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCT‑GACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGa                             >  2:1614280/1‑150 (MQ=255)

                                                                                                                        gTCGAGCAGTACGGCGCTGTCGCCGTGCTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTgg                              >  1:2467818/1‑150 (MQ=39)

                                                                                                                           gAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACt                           >  1:2089688/1‑150 (MQ=255)

                                                                                                                                gCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCa                      >  2:3272353/1‑150 (MQ=255)

                                                                                                                                 cACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCaa                     >  1:1863869/1‑150 (MQ=255)

                                                                                                                                   cGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAgg                   >  1:4348620/1‑150 (MQ=255)

                                                                                                                                    ggCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGc                  <  2:2153122/150‑1 (MQ=255)

                                                                                                                                    ggCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGc                  <  1:2021288/150‑1 (MQ=255)

                                                                                                                                      cGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCt                <  1:5157454/150‑1 (MQ=255)

                                                                                                                                      cGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCt                <  2:4137387/150‑1 (MQ=255)

                                                                                                                                       gACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTg               <  1:533489/150‑1 (MQ=255)

                                                                                                                                            cgcCGTGCAGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTGGCACg          <  2:229466/150‑1 (MQ=255)

                                                                                                                                              ccGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTGGCACGtt        <  2:1485016/150‑1 (MQ=255)

                                                                                                                                                gTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTGGCACGTTTc      <  1:3679134/150‑1 (MQ=255)

                                                                                                                                                 tACTGCTCGACCTGTTCGGTGTGCTGCAGGGCCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTGGCACGTTTCa     <  2:3393630/150‑1 (MQ=255)

                                                                                                                                                 tACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTGGCACGTTTCa     <  2:1629007/150‑1 (MQ=255)

                                                                                                                                                    tGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTc                          <  1:1574993/126‑1 (MQ=255)

GGCCTGATGCTCGCCGCCCATTGGCAGGGCGAGGTCGACTGCCGCCTGCGCGCCCCCGCCGGTAGCCTGGCGCAACTGGCCTTGGTCAAGGACAAGACCGCTGTGCTGCACAGCCCGCAGGTCGAGCTGCACGGCGACAGCGCCGTACTGCTCGACCTGTTCGGTGTGCTGCAGGACCTGGAGCTGGACTGGGAGCACGAGCTGCAACGCTGGCTTGGCCCGGTCGCCACGGCAATGCTGGCCGGCCATATCCGCCTGCGCGCTCGCTGGACTCGCCAAGGCCTGGCACGTTTCAGCC  >  NC_002947/5714072‑5714369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`