BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,737,942	C→G	5.2%	V494V (GTG→GTC)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,737,942	0	C	G	5.2%	521.2 / 13.4	155	V494V (GTG→GTC)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base C (72/75); new base G (5/3); total (77/78)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 159 total aligned reads displayed.

AGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAG  >  NC_002947/5737794‑5738091

aGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCa                                                                                                                                                      <  1:3006522/150‑1 (MQ=255)

  tCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACg                                                                                                                                                    >  2:1161176/1‑150 (MQ=255)

  tCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACg                                                                                                                                                    >  1:3218485/1‑150 (MQ=255)

    gATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGcc                                                                                                                                                  >  2:2193900/1‑150 (MQ=255)

      tGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCAc                                                                                                                                                <  1:3650610/150‑1 (MQ=255)

       ggCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCAcc                                                                                                                                               <  1:3857507/150‑1 (MQ=255)

        gCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCg                                                                                                                                              >  1:3989741/1‑150 (MQ=255)

        gCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCg                                                                                                                                              <  1:859823/150‑1 (MQ=255)

                 tAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGc                                                                                                                                     >  1:5344781/1‑150 (MQ=255)

                  aaCCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCa                                                                                                                                    >  2:5229858/1‑150 (MQ=255)

                    ccGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGc                                                                                                                                  <  2:5057863/150‑1 (MQ=255)

                     cGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCg                                                                                                                                 >  1:1341183/1‑150 (MQ=255)

                       gCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGAt                                                                                                                               >  2:4976142/1‑150 (MQ=255)

                         aTCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATAc                                                                                                                             >  1:4337675/1‑150 (MQ=255)

                          tCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATAcc                                                                                                                            >  2:960170/1‑150 (MQ=255)

                             gCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAg                                                                                                                         <  2:5156582/150‑1 (MQ=255)

                               gTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGt                                                                                                                       <  2:3727067/150‑1 (MQ=255)

                                 gACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTgg                                                                                                                     >  1:5150376/1‑150 (MQ=255)

                                    gCATGACGCCAGCCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGgcgc                                                                                                                  >  2:3862453/1‑150 (MQ=255)

                                      aTGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGcgccg                                                                                                                >  1:3722182/1‑150 (MQ=255)

                                       tGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGcgccgc                                                                                                               >  1:5375208/1‑150 (MQ=255)

                                           gCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGc                                                                                                           >  2:3229312/1‑150 (MQ=255)

                                            ccAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCg                                                                                                          <  1:946887/150‑1 (MQ=255)

                                            ccAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGAGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCg                                                                                                          >  1:144064/1‑150 (MQ=255)

                                              aGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGgt                                                                                                        <  1:3570347/150‑1 (MQ=255)

                                                  ccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTc                                                                                                    <  1:564798/150‑1 (MQ=255)

                                                  ccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTc                                                                                                    <  1:1513725/150‑1 (MQ=255)

                                                   cTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTca                                                                                                   >  2:4233215/1‑150 (MQ=255)

                                                   cTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTca                                                                                                   >  2:2178704/1‑150 (MQ=255)

                                                     gggTCGTTGGTGAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGTCGATGACCTTGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                                 <  2:2844542/150‑1 (MQ=255)

                                                     gggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                                 >  1:3258607/1‑150 (MQ=255)

                                                     gggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                                 <  2:2288288/150‑1 (MQ=255)

                                                      ggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagca                                                                                                <  1:2804037/150‑1 (MQ=255)

                                                       gTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagcag                                                                                               >  2:3429289/1‑150 (MQ=255)

                                                       gTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagcag                                                                                               <  2:4053406/150‑1 (MQ=255)

                                                        tCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAgg                                                                                              <  2:3294865/150‑1 (MQ=255)

                                                          gTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGc                                                                                            >  1:3774241/1‑150 (MQ=255)

                                                            tGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGt                                                                                          <  2:1098507/150‑1 (MQ=255)

                                                              gTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTg                                                                                        >  1:2447543/1‑150 (MQ=255)

                                                               tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                       >  2:1155687/1‑150 (MQ=255)

                                                                cAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGag                                                                                      >  1:4665054/1‑150 (MQ=255)

                                                                 aGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagc                                                                                     >  1:2829432/1‑150 (MQ=255)

                                                                 aGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagc                                                                                     >  1:4949153/1‑150 (MQ=255)

                                                                    aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                                  <  2:4382334/150‑1 (MQ=255)

                                                                    aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                                  <  2:5280665/150‑1 (MQ=255)

                                                                            gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                                          <  1:4042639/150‑1 (MQ=255)

                                                                            gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                                          >  1:886991/1‑150 (MQ=255)

                                                                               gcgcTGGTCGGCATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGa                                                                       <  2:571286/150‑1 (MQ=255)

                                                                                 gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                     >  2:843330/1‑150 (MQ=255)

                                                                                 gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                     >  1:805063/1‑150 (MQ=255)

                                                                                  cTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAAc                                                                    <  1:1201244/150‑1 (MQ=255)

                                                                                   tCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACa                                                                   >  1:773737/1‑150 (MQ=255)

                                                                                       cggcCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGt                                                               <  1:4238729/150‑1 (MQ=255)

                                                                                       cggcCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGt                                                               <  1:4238653/150‑1 (MQ=255)

                                                                                           cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                                           >  1:4482510/1‑150 (MQ=255)

                                                                                           cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                                           <  2:2034498/150‑1 (MQ=255)

                                                                                           cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                                           <  2:4772313/150‑1 (MQ=255)

                                                                                            tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGTCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                          >  2:3706659/1‑150 (MQ=255)

                                                                                            tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                          >  2:3338062/1‑150 (MQ=255)

                                                                                               tCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCa                                                       <  2:1777024/150‑1 (MQ=255)

                                                                                                cGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCAt                                                      >  1:5239833/1‑150 (MQ=255)

                                                                                                cGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCAt                                                      <  1:2059469/150‑1 (MQ=255)

                                                                                                  gTACCGTCGCAGACGATGACCATGCCCGAGTGCAGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCa                                                    <  2:3407034/150‑1 (MQ=255)

                                                                                                    aCCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACg                                                  >  2:2426772/1‑150 (MQ=255)

                                                                                                      cGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCa                                                <  1:2084740/150‑1 (MQ=255)

                                                                                                        tCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATg                                              <  2:4230949/150‑1 (MQ=255)

                                                                                                        tCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATg                                              <  1:174068/150‑1 (MQ=255)

                                                                                                         cGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCg                                                                                           <  2:3915655/104‑1 (MQ=255)

                                                                                                         cGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATgg                                             <  2:3216400/150‑1 (MQ=255)

                                                                                                              cagATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTcttt                                                  <  2:2569331/138‑4 (MQ=255)

                                                                                                              cagATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTcttt                                                  >  1:2569331/3‑137 (MQ=255)

                                                                                                              aCGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCg                                        <  1:314957/150‑1 (MQ=255)

                                                                                                                     ccATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTCTTTCATGGATTCGGTTTcac                                 >  1:3207414/1‑148 (MQ=255)

                                                                                                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                <  2:1065557/150‑1 (MQ=255)

                                                                                                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                >  1:3228503/1‑150 (MQ=255)

                                                                                                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                <  2:3576568/150‑1 (MQ=255)

                                                                                                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                <  2:3218485/150‑1 (MQ=255)

                                                                                                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                >  2:1931350/1‑150 (MQ=255)

                                                                                                                         gCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGtt                             <  1:1278841/150‑1 (MQ=255)

                                                                                                                         gCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGtt                             <  1:2193900/150‑1 (MQ=255)

                                                                                                                           ccGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCg                           >  1:4386620/1‑150 (MQ=255)

                                                                                                                             gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg                         <  1:1161176/150‑1 (MQ=255)

                                                                                                                              aGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGc                        <  1:4029928/150‑1 (MQ=255)

                                                                                                                                   tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                   >  1:2868255/1‑150 (MQ=255)

                                                                                                                                   tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                   >  1:4808556/1‑150 (MQ=255)

                                                                                                                                   tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                   >  1:2082210/1‑150 (MQ=255)

                                                                                                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                 <  1:2537486/150‑1 (MQ=255)

                                                                                                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                 <  1:2537453/150‑1 (MQ=255)

                                                                                                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                 <  1:3862453/150‑1 (MQ=255)

                                                                                                                                      gagaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGGGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAcc                <  1:3449227/150‑1 (MQ=255)

                                                                                                                                       agaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCg               >  1:2572102/1‑150 (MQ=255)

                                                                                                                                       agaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCg               >  1:4722035/1‑150 (MQ=255)

                                                                                                                                           gCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGcc           >  2:4566559/1‑150 (MQ=255)

                                                                                                                                           gCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGcc           <  1:4642104/150‑1 (MQ=255)

                                                                                                                                              cATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGa        >  1:2704778/1‑150 (MQ=255)

                                                                                                                                              cATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGa        >  2:4528876/1‑150 (MQ=255)

                                                                                                                                               aTGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAg       >  2:3063647/1‑150 (MQ=255)

                                                                                                                                                   ccACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGa   <  2:3989838/150‑1 (MQ=255)

                                                                                                                                                   ccACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGa   <  2:3989741/150‑1 (MQ=255)

                                                                                                                                                    cACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAg  <  2:4722866/150‑1 (MQ=255)

AGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAG  >  NC_002947/5737794‑5738091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`