BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,737,993	C→G	5.4%	L477L (CTG→CTC)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,737,993	0	C	G	5.4%	495.6 / 13.6	148	L477L (CTG→CTC)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base C (70/70); new base G (5/3); total (75/73)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 160 total aligned reads displayed.

CCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACC  >  NC_002947/5737844‑5738140

ccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTc                                                                                                                                                     <  1:1513725/150‑1 (MQ=255)

ccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTc                                                                                                                                                     <  1:564798/150‑1 (MQ=255)

 cTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTca                                                                                                                                                    >  2:4120791/1‑150 (MQ=255)

   gggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                                                                                  >  1:3258607/1‑150 (MQ=255)

    ggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagca                                                                                                                                                 <  1:2804037/150‑1 (MQ=255)

     gTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagcag                                                                                                                                                >  2:3429289/1‑150 (MQ=255)

        gTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGc                                                                                                                                             >  1:3774241/1‑150 (MQ=255)

         ttGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCg                                                                                                                                            >  2:3099047/1‑150 (MQ=255)

          tGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGt                                                                                                                                           <  2:1098507/150‑1 (MQ=255)

            gTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTg                                                                                                                                         >  1:2447543/1‑150 (MQ=255)

             tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                                                                        >  2:1155687/1‑150 (MQ=255)

             tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                                                                        <  2:3266998/150‑1 (MQ=255)

               aGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagc                                                                                                                                      >  1:4949153/1‑150 (MQ=255)

               aGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagc                                                                                                                                      >  1:2829432/1‑150 (MQ=255)

                  aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                                                                                   <  2:4382334/150‑1 (MQ=255)

                          gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                                                                                           <  1:4042639/150‑1 (MQ=255)

                           aTGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCg                                                                                                                          <  2:5150376/150‑1 (MQ=255)

                              cgcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTGGAGCAGCGGCCAGTCGGac                                                                                                                       >  2:4556017/1‑149 (MQ=255)

                               gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                                      >  2:843330/1‑150 (MQ=255)

                               gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                                      >  1:805063/1‑150 (MQ=255)

                                cTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAAc                                                                                                                     <  1:1201244/150‑1 (MQ=255)

                                 tCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACa                                                                                                                    >  1:773737/1‑150 (MQ=255)

                                   gtcggcATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGc                                                                                                                  >  1:3789756/3‑150 (MQ=255)

                                   ggcggcCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGc                                                                                                                  <  1:3616891/150‑1 (MQ=255)

                                     cggcCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGt                                                                                                                <  1:4238729/150‑1 (MQ=255)

                                       gcCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTcc                                                                                                              <  2:4819430/150‑1 (MQ=255)

                                         cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                                                                                            >  1:4482510/1‑150 (MQ=255)

                                         cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                                                                                            <  2:2034498/150‑1 (MQ=255)

                                          tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGTCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                                                                           >  2:3706659/1‑150 (MQ=255)

                                          tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                                                                           >  2:3338062/1‑150 (MQ=255)

                                          tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                                                                           >  2:4240856/1‑150 (MQ=255)

                                            ttccgaaCCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGcc                                                                                                         >  1:5306458/7‑150 (MQ=255)

                                             tCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCa                                                                                                        >  2:2118177/1‑150 (MQ=255)

                                              cGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCAt                                                                                                       <  1:2059469/150‑1 (MQ=255)

                                               ggTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATc                                                                                                      <  2:5375208/150‑1 (MQ=255)

                                                gaaCCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGTAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTct                                                                                                     >  2:4666671/3‑149 (MQ=255)

                                                gTACCGTCGCAGACGATGACCATGCCCGAGTGCAGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCa                                                                                                     <  2:3407034/150‑1 (MQ=255)

                                                  aCCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACg                                                                                                   >  2:2426772/1‑150 (MQ=255)

                                                    cGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCa                                                                                                 <  1:2084740/150‑1 (MQ=255)

                                                    cGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCa                                                                                                 <  2:3228570/150‑1 (MQ=255)

                                                      tCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATg                                                                                               <  2:4230949/150‑1 (MQ=255)

                                                      tCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATg                                                                                               <  1:174068/150‑1 (MQ=255)

                                                       cGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCg                                                                                                                                            <  2:3915655/104‑1 (MQ=255)

                                                            cagATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTcttt                                                                                                   <  2:2569331/138‑4 (MQ=255)

                                                            cagATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTcttt                                                                                                   >  1:2569331/3‑137 (MQ=255)

                                                            aCGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCg                                                                                         <  1:314957/150‑1 (MQ=255)

                                                            aCGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCg                                                                                         >  2:2525114/1‑150 (MQ=255)

                                                              gATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGt                                                                                       <  2:3342157/150‑1 (MQ=255)

                                                                   ccATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTCTTTCATGGATTCGGTTTcac                                                                                  >  1:3207414/1‑148 (MQ=255)

                                                                    cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                                 >  2:1931350/1‑150 (MQ=255)

                                                                    cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                                 <  2:3218485/150‑1 (MQ=255)

                                                                    cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                                 >  1:3228503/1‑150 (MQ=255)

                                                                    cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                                 <  2:3576568/150‑1 (MQ=255)

                                                                    cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                                 >  1:3228499/1‑150 (MQ=255)

                                                                       gCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGtt                                                                              <  1:1278841/150‑1 (MQ=255)

                                                                         ccGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCg                                                                            <  1:2525114/150‑1 (MQ=255)

                                                                          cGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCgg                                                                           >  2:2277360/1‑150 (MQ=255)

                                                                           gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg                                                                          <  1:1161176/150‑1 (MQ=255)

                                                                            aGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGc                                                                         <  1:4029928/150‑1 (MQ=255)

                                                                                 tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                                                                    >  1:2082293/1‑150 (MQ=255)

                                                                                 tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                                                                    >  1:2868255/1‑150 (MQ=255)

                                                                                   cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                                  <  1:2537486/150‑1 (MQ=255)

                                                                                    gagaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGGGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAcc                                                                 <  1:3449227/150‑1 (MQ=255)

                                                                                     agaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCg                                                                >  1:4722035/1‑150 (MQ=255)

                                                                                     agaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCg                                                                >  1:2572102/1‑150 (MQ=255)

                                                                                         gCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGcc                                                            <  1:4642104/150‑1 (MQ=255)

                                                                                         gCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGcc                                                            >  2:4566559/1‑150 (MQ=255)

                                                                                             aTGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAg                                                        >  2:3063647/1‑150 (MQ=255)

                                                                                                 ccACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGa                                                    <  2:3989838/150‑1 (MQ=255)

                                                                                                 acACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGTGCAGCGGCCAGTCGGACACAGCGTCCGAACCATCCCGCATGGCCTCGGGTTCGGGGTTCGGGCGGGATTGCGTGCCGGTGTCgg                                                    >  2:3962883/2‑149 (MQ=255)

                                                                                                  cACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAg                                                   <  2:4722866/150‑1 (MQ=255)

                                                                                                   aCGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAgg                                                  >  2:3708379/1‑150 (MQ=255)

                                                                                                     gCCACCGCCATGGTGCAGCGATTCCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGTGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggtg                                                <  2:4284498/150‑1 (MQ=255)

                                                                                                     gCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggtg                                                <  1:1754748/150‑1 (MQ=255)

                                                                                                     gCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggtg                                                >  2:4878649/1‑150 (MQ=255)

                                                                                                     gCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggtg                                                >  2:4878646/1‑150 (MQ=255)

                                                                                                      ccACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggtgg                                               >  2:2629718/1‑150 (MQ=255)

                                                                                                         ccGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCa                                            >  2:2448987/1‑150 (MQ=255)

                                                                                                           gCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGGAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACg                                          >  1:1040255/1‑150 (MQ=255)

                                                                                                               tGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCg                                      >  1:4247266/1‑150 (MQ=255)

                                                                                                                  tGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATc                                   <  1:4942476/150‑1 (MQ=255)

                                                                                                                  tGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATc                                   >  1:4206458/1‑150 (MQ=255)

                                                                                                                     aGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcg                                                                                   <  2:1670095/99‑1 (MQ=255)

                                                                                                                             ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGc                        >  1:1460337/1‑150 (MQ=255)

                                                                                                                             ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGc                        >  2:802018/1‑150 (MQ=255)

                                                                                                                             ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGc                        <  1:5208431/150‑1 (MQ=255)

                                                                                                                              taGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGcc                       >  1:3893343/2‑150 (MQ=255)

                                                                                                                                   ggCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAg                  <  2:1409668/150‑1 (MQ=255)

                                                                                                                                    gcgcCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGTAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCa                                            <  2:762044/123‑1 (MQ=255)

                                                                                                                                    gcgcCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGc                 >  2:5202126/1‑150 (MQ=255)

                                                                                                                                      gccgccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc               >  2:3020100/1‑150 (MQ=255)

                                                                                                                                      gccgccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc               >  1:1006310/1‑150 (MQ=255)

                                                                                                                                      gccgccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc               >  2:5338209/1‑150 (MQ=255)

                                                                                                                                      gccgccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc               >  1:5180529/1‑150 (MQ=255)

                                                                                                                                          ccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCTGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTcgccg           <  1:4233215/150‑1 (MQ=255)

                                                                                                                                          ccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTcgccg           >  2:2287480/1‑150 (MQ=255)

                                                                                                                                            tGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCt         >  1:4140663/1‑150 (MQ=255)

                                                                                                                                                 tgtTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGa    >  1:988268/1‑150 (MQ=255)

                                                                                                                                                   ttCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGAcc  >  2:5450635/1‑150 (MQ=255)

                                                                                                                                                   ttCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGAcc  >  1:707215/1‑150 (MQ=255)

CCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACC  >  NC_002947/5737844‑5738140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`