BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	433,865	T→A	6.7%	A446A (GCA→GCT)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	433,865	0	T	A	6.7%	732.3 / 30.2	223	A446A (GCA→GCT)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base T (104/103); new base A (10/5); total (115/108)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 226 total aligned reads displayed.

GACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTT  >  NC_002947/433718‑434014

     tgttgttTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGa                                                                                                                                                >  1:3191725/1‑150 (MQ=255)

         gttTTCGTAGGCGCCGTTCGACTTCTCGTTCTTCATGGCTCCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTTGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTTTTGATGAACACCACGCGCTCGGCTGCTGCCTTGATGCa                                                                                                                                            <  1:4384162/150‑1 (MQ=255)

         gttTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCa                                                                                                                                            <  1:5434282/150‑1 (MQ=255)

         gttTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCa                                                                                                                                            <  1:2251670/150‑1 (MQ=255)

          ttttCGTAGGCGCCGATCCACGTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAg                                                                                                                                           <  1:3521968/150‑1 (MQ=255)

                tAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTg                                                                                                                                     <  1:299451/150‑1 (MQ=255)

                tAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTg                                                                                                                                     <  1:2654988/150‑1 (MQ=255)

                 aGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                                    <  2:1121044/150‑1 (MQ=255)

                   gcgcCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCtt                                                                                                                                                  >  1:1079679/1‑134 (MQ=255)

                   gcgcCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCtt                                                                                                                                                  <  2:1079679/134‑1 (MQ=255)

                    cgcCGATCCACGTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGt                                                                                                                                 <  2:4265317/150‑1 (MQ=255)

                     gcCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                                    <  2:1001194/146‑1 (MQ=255)

                     gcCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                                    >  1:1001194/1‑146 (MQ=255)

                             aCTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTgg                                                                                                                        >  1:4825029/1‑150 (MQ=255)

                             aCTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTgg                                                                                                                        >  2:5251049/1‑150 (MQ=255)

                             aCTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTgg                                                                                                                        >  1:4549856/1‑150 (MQ=255)

                                     ttcttcATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGa                                                                                                                              <  1:1381307/136‑1 (MQ=255)

                                         tcATGTCGCCCTTGCGGATCATCGGGCCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCt                                                                                                            >  2:4672319/1‑150 (MQ=255)

                                           aTGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCg                                                                                                          >  2:5433892/1‑150 (MQ=255)

                                             ggCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTc                                                                                                        <  1:1240329/150‑1 (MQ=255)

                                             ggCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTc                                                                                                        <  1:1811072/150‑1 (MQ=255)

                                               cACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCa                                                                                                      <  2:1520211/150‑1 (MQ=255)

                                                aCCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCat                                                                                                     >  2:1931543/1‑150 (MQ=255)

                                                   tttGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCatgat                                                                                                  >  2:4944688/1‑150 (MQ=255)

                                                      gcgcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGcc                                                                                               <  1:4944659/150‑1 (MQ=255)

                                                        gcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGa                                                                                             >  2:2781008/1‑150 (MQ=255)

                                                        gcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGa                                                                                             <  1:4307324/150‑1 (MQ=255)

                                                          accacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGAcc                                                                                           <  2:1388522/150‑1 (MQ=255)

                                                           ccacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCt                                                                                          <  2:1822831/150‑1 (MQ=255)

                                                              cGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGa                                                                                       <  2:4087711/150‑1 (MQ=255)

                                                                 cgcCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACgg                                                                                    <  1:2028319/150‑1 (MQ=255)

                                                                  gcCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTc                                                                                                              >  2:4385899/1‑123 (MQ=255)

                                                                   ccGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGgtg                                                                                  <  1:5251049/150‑1 (MQ=255)

                                                                    cGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGgtgt                                                                                 >  2:4939896/1‑150 (MQ=255)

                                                                     ggCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGtt                                                                                <  2:944505/150‑1 (MQ=255)

                                                                        ttCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGTCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTgcg                                                                             >  1:382709/1‑150 (MQ=255)

                                                                           cATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCgg                                                                          >  1:3625430/1‑150 (MQ=255)

                                                                           cATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCgg                                                                          <  2:611512/150‑1 (MQ=255)

                                                                           cATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCgg                                                                          >  2:5415529/1‑150 (MQ=255)

                                                                            aTCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGc                                                                         >  2:2776907/1‑150 (MQ=255)

                                                                               gAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATg                                                                      >  2:4205425/1‑150 (MQ=255)

                                                                                aGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGc                                                                     <  2:1379755/150‑1 (MQ=255)

                                                                                  gtgtGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGcc                                                                    >  2:533678/1‑149 (MQ=255)

                                                                                  gtgtGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGcc                                                                    <  1:533678/149‑1 (MQ=255)

                                                                                   tgtgGATTTCATCGCCAGTGCGGTCAAGGTAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGTGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCca                                                                  >  2:385502/1‑150 (MQ=255)

                                                                                   tgtgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCa                                                                                                                                            >  2:2113791/1‑76 (MQ=255)

                                                                                    gtgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcag                                                                 >  2:2492352/1‑150 (MQ=255)

                                                                                      ggATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagca                                                               <  2:515348/150‑1 (MQ=255)

                                                                                       gATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCaagcag                                                              >  1:160502/1‑150 (MQ=255)

                                                                                       gATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagcag                                                              >  1:3923005/1‑150 (MQ=255)

                                                                                        aTTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAgg                                                             >  2:476326/1‑150 (MQ=255)

                                                                                         tttCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAggc                                                            >  2:1120062/1‑149 (MQ=255)

                                                                                          ttCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTc                                                                                                              >  1:3288647/1‑99 (MQ=255)

                                                                                          ttCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTc                                                                                                              <  2:3288647/99‑1 (MQ=255)

                                                                                           tCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCt                                                          >  1:1189535/1‑150 (MQ=255)

                                                                                              tCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCg                                                       >  1:2956846/1‑150 (MQ=255)

                                                                                              tCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCg                                                       >  1:545625/1‑150 (MQ=255)

                                                                                               cGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGa                                                      >  1:2413988/1‑150 (MQ=255)

                                                                                                gCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCggt                                                     >  2:2346006/1‑148 (MQ=255)

                                                                                                 ccAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCggtg                                                    >  1:1863008/1‑147 (MQ=255)

                                                                                                    gTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                                                 <  1:1469478/150‑1 (MQ=255)

                                                                                                    gTGCGGTCAAGGAAGCCGGGGTTGCTGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                                                 <  1:2581345/150‑1 (MQ=255)

                                                                                                      gCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCt                                               <  1:3478712/150‑1 (MQ=255)

                                                                                                      gCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCt                                               <  1:1451827/150‑1 (MQ=255)

                                                                                                        ggTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGa                                             <  1:1234482/150‑1 (MQ=255)

                                                                                                          tCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAg                                           <  2:1705585/150‑1 (MQ=255)

                                                                                                          tCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAg                                           >  1:4388050/1‑150 (MQ=255)

                                                                                                           cAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGCCTTCGACCTGGCTGAAGa                                          <  1:1120062/150‑1 (MQ=255)

                                                                                                            aaGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAt                                         >  1:4066354/1‑150 (MQ=255)

                                                                                                            aaGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAt                                         <  1:89473/150‑1 (MQ=255)

                                                                                                              ggAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtct                                       >  1:2716422/1‑150 (MQ=255)

                                                                                                              ggAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtct                                       >  2:364522/1‑150 (MQ=255)

                                                                                                              ggAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtct                                       >  2:796865/1‑150 (MQ=255)

                                                                                                                  gCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGt                                   <  2:2754275/150‑1 (MQ=255)

                                                                                                                  gCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGt                                   >  2:3706390/1‑150 (MQ=255)

                                                                                                                   ccGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTg                                  <  1:4970501/150‑1 (MQ=255)

                                                                                                                    cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACg                                                                                     >  2:1768523/1‑98 (MQ=255)

                                                                                                                    cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACg                                                                                     <  1:1768360/98‑1 (MQ=255)

                                                                                                                    cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTgg                                 <  2:4762062/150‑1 (MQ=255)

                                                                                                                     ggTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGc                                <  1:4004692/150‑1 (MQ=255)

                                                                                                                      gtgtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCg                               >  2:5007949/1‑150 (MQ=255)

                                                                                                                           gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                          <  1:1058041/150‑1 (MQ=255)

                                                                                                                           gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                          >  2:2810369/1‑150 (MQ=255)

                                                                                                                             tgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCa                        >  1:5280573/1‑150 (MQ=255)

                                                                                                                             tgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCa                        >  1:4721370/1‑150 (MQ=255)

                                                                                                                                acacCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACtt                     >  2:969283/1‑150 (MQ=255)

                                                                                                                                 caccacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTc                    <  2:3191725/150‑1 (MQ=255)

                                                                                                                                   ccacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCt                  <  1:254494/150‑1 (MQ=255)

                                                                                                                                   ccacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCt                  >  1:4091309/1‑150 (MQ=255)

                                                                                                                                    cacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTc                 >  2:3204296/1‑150 (MQ=255)

                                                                                                                                    cacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTc                 >  1:3963284/1‑150 (MQ=255)

                                                                                                                                     acGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCa                <  1:3877409/150‑1 (MQ=255)

                                                                                                                                      cgcgCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAg               >  1:594088/1‑150 (MQ=255)

                                                                                                                                       gcgcTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAgg              >  1:2292340/1‑150 (MQ=255)

                                                                                                                                           tCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCg          >  1:1254884/1‑150 (MQ=255)

                                                                                                                                           tCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCg          >  2:3953737/1‑150 (MQ=255)

                                                                                                                                            cggcggCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGt         <  2:2292340/150‑1 (MQ=255)

                                                                                                                                             ggcggcTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTg        >  2:158781/1‑150 (MQ=255)

                                                                                                                                              gcggcTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGc       <  1:4182858/150‑1 (MQ=255)

                                                                                                                                                   tGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGtt                                                                                <  1:2388967/72‑1 (MQ=255)

GACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTT  >  NC_002947/433718‑434014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`