BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,739,001	G→A	16.7%	G141G (GGC→GGT)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,739,001	0	G	A	16.7%	445.8 / 75.1	191	G141G (GGC→GGT)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base G (67/92); new base A (13/19); total (80/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 194 total aligned reads displayed.

CCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAA  >  NC_002947/5738854‑5739150

cccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCCCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTGCGACGACGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGcc                                                                                                                                                     >  2:3865902/1‑150 (MQ=255)

cccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGcc                                                                                                                                                     >  2:904652/1‑150 (MQ=255)

cccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGcc                                                                                                                                                     >  1:1231539/1‑150 (MQ=255)

cccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGcc                                                                                                                                                     >  1:2173986/1‑150 (MQ=255)

  cagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGa                                                                                                                                                   >  2:4253847/1‑150 (MQ=255)

  cagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGa                                                                                                                                                   >  2:4078441/1‑150 (MQ=255)

  cagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGa                                                                                                                                                   >  1:3232641/1‑150 (MQ=255)

  cagccagGGTCGCGGCCAGGGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGGACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGa                                                                                                                                                   <  2:5275251/150‑1 (MQ=255)

   agccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGAt                                                                                                                                                  <  1:4373523/150‑1 (MQ=255)

     ccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGt                                                                                                                                                <  1:1296976/150‑1 (MQ=255)

     ccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGt                                                                                                                                                >  2:1718948/1‑150 (MQ=255)

       agGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAg                                                                                                                                              >  2:1895048/1‑150 (MQ=255)

           tCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATcc                                                                                                                                          <  2:1455628/150‑1 (MQ=255)

             gcgGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAg                                                                                                                                        >  2:1583450/1‑150 (MQ=255)

             gcgGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGGGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAg                                                                                                                                        <  1:2904016/150‑1 (MQ=255)

                 ccAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGc                                                                                                                                    <  2:2421720/150‑1 (MQ=255)

                       gCTGGGCTCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcgg                                                                                                                              <  2:1788904/150‑1 (MQ=255)

                         tGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTc                                                                                                                            >  1:3843481/1‑150 (MQ=255)

                           ggCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCAt                                                                                                                          <  1:2105733/150‑1 (MQ=255)

                                cgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGc                                                                                                                     <  1:3403037/150‑1 (MQ=255)

                                cgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGc                                                                                                                     >  2:3016421/1‑150 (MQ=255)

                                  tgggcggcgccCAGGCCAGCGGTGAGTACCCACTTGCATTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCa                                                                                                                   <  1:2468546/143‑1 (MQ=255)

                                   ccATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCAt                                                                                                                  <  2:1162885/150‑1 (MQ=255)

                                     aTGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATAc                                                                                                                >  1:721701/1‑150 (MQ=255)

                                     aTGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGGGCTGGCGACCGGCTTCGACGAAGGTGTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATAc                                                                                                                >  2:648363/1‑150 (MQ=255)

                                        ccgccCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGGTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATc                                                                                                             <  2:3309927/150‑1 (MQ=255)

                                            ccAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcgg                                                                                                                                 <  2:4102421/126‑1 (MQ=255)

                                             cAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAg                                                                                                        <  1:2479761/150‑1 (MQ=255)

                                              aGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAgg                                                                                                       <  2:2476077/150‑1 (MQ=255)

                                                gCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCGATGTATGGCCAgatg                                                                                                     <  2:295879/150‑4 (MQ=255)

                                                    gCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTg                                                                                                 <  2:146522/150‑1 (MQ=255)

                                                         gAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTc                                                                                            >  2:4552244/1‑150 (MQ=255)

                                                           gTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCa                                                                                          <  2:5005033/150‑1 (MQ=255)

                                                           gTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCa                                                                                          <  2:3834573/150‑1 (MQ=255)

                                                             aCCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGt                                                                                        >  1:4855892/1‑150 (MQ=255)

                                                              cccACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGtt                                                                                       >  2:3932345/1‑150 (MQ=255)

                                                               ccACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                                                                                      >  1:309770/1‑150 (MQ=255)

                                                               ccACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                                                                                      >  1:5295076/1‑150 (MQ=255)

                                                                 aCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCa                                                                                          >  2:3680927/1‑144 (MQ=255)

                                                                 aCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCa                                                                                          <  1:3680927/144‑1 (MQ=255)

                                                                 aCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGt                                                                                    <  2:5383023/150‑1 (MQ=255)

                                                                 aCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGt                                                                                    <  1:906838/150‑1 (MQ=255)

                                                                   ttGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                                                                                      >  2:2708243/1‑146 (MQ=255)

                                                                   ttGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                                                                                      <  1:2708243/146‑1 (MQ=255)

                                                                     gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                                                                                <  1:253485/150‑1 (MQ=255)

                                                                      cccttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGaaa                                                                               <  1:3992904/144‑2 (MQ=255)

                                                                      cccttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGaaa                                                                               <  2:5430821/144‑2 (MQ=255)

                                                                        tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                                                                             >  2:140532/1‑150 (MQ=255)

                                                                        tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                                                                             >  2:4295256/1‑150 (MQ=255)

                                                                           cAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTc                                                                          <  2:5071676/150‑1 (MQ=255)

                                                                            aGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATcc                                                                         <  1:1107597/150‑1 (MQ=255)

                                                                            aGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTcc                                                                         <  2:2090542/150‑1 (MQ=255)

                                                                             ggCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTccc                                                                        <  1:4995542/150‑1 (MQ=255)

                                                                                        taatGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCaa                                                             >  2:4241487/4‑149 (MQ=255)

                                                                                           tGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgtgt                                                          >  2:852747/1‑150 (MQ=255)

                                                                                           tGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgtgt                                                          <  2:309770/150‑1 (MQ=255)

                                                                                           tGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgtgt                                                          >  1:4692110/1‑150 (MQ=255)

                                                                                           tGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAatgc                                                          >  2:3504344/1‑149 (MQ=255)

                                                                                             cTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTgg                                                        <  2:418503/150‑1 (MQ=255)

                                                                                               ggCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCa                                                      >  1:979187/1‑150 (MQ=255)

                                                                                                  gACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCa                                                   >  1:1058861/1‑150 (MQ=255)

                                                                                                     cGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGACCTGGCTGACGATGTAGATCCAGCTGCAGGAGGTCATCTTGCCATACATCGCCAGTCCCTTGGCGTCCAGTTCGTTGAAGTGTTCACAGTTGGCCCAGTGTGGCACCAGGt                                                >  2:1064793/1‑150 (MQ=255)

                                                                                                      ggCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGtt                                               <  1:3484325/150‑1 (MQ=255)

                                                                                                       gCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCATCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTg                                              <  1:4364817/150‑1 (MQ=255)

                                                                                                       gCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTg                                              >  2:1270436/1‑150 (MQ=255)

                                                                                                         ttCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGa                                            <  1:3932345/150‑1 (MQ=255)

                                                                                                            gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                         <  1:4210459/150‑1 (MQ=255)

                                                                                                            gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                         >  2:1896529/1‑150 (MQ=255)

                                                                                                             acgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTg                                        <  1:2845741/150‑1 (MQ=255)

                                                                                                             acgaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTg                                        >  2:2680646/1‑150 (MQ=255)

                                                                                                             acgaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTg                                        <  2:5322950/150‑1 (MQ=255)

                                                                                                              cgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTgg                                       <  2:5337493/150‑1 (MQ=255)

                                                                                                              cgaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGACATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTgg                                       >  1:648506/1‑150 (MQ=255)

                                                                                                               gaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGc                                      >  1:2811767/1‑150 (MQ=255)

                                                                                                               gaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAAtt                                         >  1:2620930/1‑147 (MQ=255)

                                                                                                                 aGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCaa                                    <  2:1711976/150‑1 (MQ=255)

                                                                                                                    tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                 <  1:1466514/150‑1 (MQ=255)

                                                                                                                    tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                 <  2:4573303/150‑1 (MQ=255)

                                                                                                                    tttCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGAtga                                 <  2:29860/150‑3 (MQ=255)

                                                                                                                        aTAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcg                                                                                                                               >  2:3231231/1‑52 (MQ=255)

                                                                                                                        aTAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAc                             >  1:868173/1‑150 (MQ=255)

                                                                                                                             tGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAcgcgcg                        >  2:4860935/1‑150 (MQ=255)

                                                                                                                             tGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAcgcgcg                        >  2:29619/1‑150 (MQ=255)

                                                                                                                             tGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAAcgcgcg                        <  2:4173301/150‑1 (MQ=255)

                                                                                                                              gCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCa                              <  1:2253135/143‑1 (MQ=255)

                                                                                                                              gCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCa                              >  2:4032880/1‑143 (MQ=255)

                                                                                                                              gCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCa                              >  2:2253135/1‑143 (MQ=255)

                                                                                                                               cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                      <  2:2173986/150‑1 (MQ=255)

                                                                                                                                 cTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGc                    <  2:2502212/150‑1 (MQ=255)

                                                                                                                                 cTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGcc                                                                >  2:702416/1‑106 (MQ=255)

                                                                                                                                      cGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGc               <  1:639685/150‑1 (MQ=255)

                                                                                                                                      cGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGc               >  1:2398130/1‑150 (MQ=255)

                                                                                                                                       gATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCt              >  1:809932/1‑150 (MQ=255)

                                                                                                                                         tGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCtgt            <  1:1699371/150‑1 (MQ=255)

                                                                                                                                          gCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCtgtg           <  1:4078441/150‑1 (MQ=255)

                                                                                                                                           cccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTgg          <  1:2707887/150‑1 (MQ=255)

                                                                                                                                              tGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTc       >  1:1281478/1‑150 (MQ=255)

                                                                                                                                                 cTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTg    <  1:1895048/150‑1 (MQ=255)

                                                                                                                                                   gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa  <  1:3633068/150‑1 (MQ=255)

                                                                                                                                                   gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa  <  1:896111/150‑1 (MQ=255)

CCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAA  >  NC_002947/5738854‑5739150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`