BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,739,018	T→A	19.6%	S136C (AGC→TGC) ‡	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,739,018	0	T	A	19.6%	447.8 / 101.4	194	S136C (AGC→TGC) ‡	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base T (68/88); new base A (17/21); total (85/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 194 total aligned reads displayed.

CCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTT  >  NC_002947/5738871‑5739165

     ggCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcg                                                                                                                                              <  2:143477/150‑1 (MQ=255)

        tGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTc                                                                                                                                           >  1:3843481/1‑150 (MQ=255)

          ggCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCAt                                                                                                                                         <  1:2105733/150‑1 (MQ=255)

               cgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGc                                                                                                                                    >  2:3016421/1‑150 (MQ=255)

                gccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGcc                                                                                                                                   <  1:3703838/150‑1 (MQ=255)

                 tgggcggcgccCAGGCCAGCGGTGAGTACCCACTTGCATTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCa                                                                                                                                  <  1:2468546/143‑1 (MQ=255)

                  ccATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCAt                                                                                                                                 <  2:1162885/150‑1 (MQ=255)

                       gcgccAAGGCCACCGGTCAGAATCCATTTGCCCTTGTGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACAtg                                                                                                                            >  2:4664198/2‑149 (MQ=255)

                       ccgccCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGGTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATc                                                                                                                            <  2:3309927/150‑1 (MQ=255)

                       ccgccCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATc                                                                                                                            <  2:65678/150‑1 (MQ=255)

                         gccCAGGCCAGCGGTGGGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGc                                                                                                                          <  2:1538666/150‑1 (MQ=255)

                           ccAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcgg                                                                                                                                                <  2:4102421/126‑1 (MQ=255)

                           ccAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcgg                                                                                                                                                >  1:4102421/1‑126 (MQ=255)

                            cAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAg                                                                                                                       <  1:2479761/150‑1 (MQ=255)

                               gCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCGATGTATGGCCAgatg                                                                                                                    <  2:295879/150‑4 (MQ=255)

                                 cAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCt                                                                                                                  >  1:2790727/1‑150 (MQ=255)

                                   ccGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCttc                                                                                                                <  1:2340628/149‑2 (MQ=255)

                                     ggTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGc                                                                                                              <  2:4015103/150‑1 (MQ=255)

                                        gAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTc                                                                                                           >  2:4552244/1‑150 (MQ=255)

                                          gTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCa                                                                                                         <  2:3834573/150‑1 (MQ=255)

                                          gTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCa                                                                                                         <  2:5005033/150‑1 (MQ=255)

                                            aCCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGt                                                                                                       >  1:4855892/1‑150 (MQ=255)

                                              ccACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                                                                                                     >  1:309698/1‑150 (MQ=255)

                                                aCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCa                                                                                                         >  2:3680927/1‑144 (MQ=255)

                                                aCTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGt                                                                                                   <  1:906838/150‑1 (MQ=255)

                                                  ttGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                                                                                                     >  2:2708243/1‑146 (MQ=255)

                                                  ttGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                                                                                                     <  1:2708243/146‑1 (MQ=255)

                                                    gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                                                                                               <  1:253485/150‑1 (MQ=255)

                                                     cccttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGaaa                                                                                              <  2:5430788/144‑2 (MQ=255)

                                                       tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTTGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCTGAGGTCATCTGGCCATACATCGCAAGGCCCATGGCGACCAGTGCGTTGAAttg                                                                                            >  1:5394533/1‑150 (MQ=255)

                                                       tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                                                                                            >  2:4295256/1‑150 (MQ=255)

                                                       tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCAAACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                                                                                            >  1:283650/1‑150 (MQ=255)

                                                           aGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTcc                                                                                        <  2:2090542/150‑1 (MQ=255)

                                                             gCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCa                                                                                      >  2:2500683/1‑150 (MQ=255)

                                                                  cgccgTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTgg                                                                                 >  2:4065780/1‑150 (MQ=255)

                                                                      gTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCtt                                                                                                                 <  1:1293611/114‑1 (MQ=255)

                                                                      gTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCtt                                                                                                                 >  2:1293611/1‑114 (MQ=255)

                                                                       taatGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCaa                                                                            >  2:4241346/4‑149 (MQ=255)

                                                                          tGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgtgt                                                                         >  2:852747/1‑150 (MQ=255)

                                                                          tGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgtgt                                                                         >  1:4692110/1‑150 (MQ=255)

                                                                          tGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgtgt                                                                         <  2:309770/150‑1 (MQ=255)

                                                                          tGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAatgc                                                                         >  2:3504344/1‑149 (MQ=255)

                                                                            cTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTgg                                                                       <  2:418503/150‑1 (MQ=255)

                                                                              ggCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCa                                                                     <  1:1620008/150‑1 (MQ=255)

                                                                              ggCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCa                                                                     >  1:979187/1‑150 (MQ=255)

                                                                                 gACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCa                                                                  >  1:1058861/1‑150 (MQ=255)

                                                                                    cGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGACCTGGCTGACGATGTAGATCCAGCTGCAGGAGGTCATCTTGCCATACATCGCCAGTCCCTTGGCGTCCAGTTCGTTGAAGTGTTCACAGTTGGCCCAGTGTGGCACCAGGt                                                               >  2:1064793/1‑150 (MQ=255)

                                                                                     ggCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGtt                                                              <  1:3484325/150‑1 (MQ=255)

                                                                                      gCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCATCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTg                                                             <  1:4364817/150‑1 (MQ=255)

                                                                                      gCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTg                                                             >  2:1270436/1‑150 (MQ=255)

                                                                                        ttCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGa                                                           <  1:171685/150‑1 (MQ=255)

                                                                                           gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                        >  2:1896529/1‑150 (MQ=255)

                                                                                           gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                        >  2:2437481/1‑150 (MQ=255)

                                                                                           gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                        <  1:4210537/150‑1 (MQ=255)

                                                                                            acgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTg                                                       <  1:3934294/150‑1 (MQ=255)

                                                                                            acgaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTg                                                       <  2:5322950/150‑1 (MQ=255)

                                                                                             cgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTgg                                                      <  2:5337493/150‑1 (MQ=255)

                                                                                             cgaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTgg                                                      <  1:3408698/150‑1 (MQ=255)

                                                                                             cgaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTgg                                                      <  1:1170298/150‑1 (MQ=255)

                                                                                              gaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGc                                                     >  1:2846065/1‑150 (MQ=255)

                                                                                              gaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAAtt                                                        <  2:2620930/147‑1 (MQ=255)

                                                                                                aGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCaa                                                   <  2:1711976/150‑1 (MQ=255)

                                                                                                  gTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATc                                                 <  1:1270436/150‑1 (MQ=255)

                                                                                                   tttCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGAtga                                                <  2:29860/150‑3 (MQ=255)

                                                                                                       aTAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcg                                                                                                                                              >  2:3231231/1‑52 (MQ=255)

                                                                                                       aTAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAc                                            >  1:868173/1‑150 (MQ=255)

                                                                                                         aGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAcgc                                          <  1:2657113/150‑1 (MQ=255)

                                                                                                            tGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAcgcgcg                                       >  2:29619/1‑150 (MQ=255)

                                                                                                            tGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAcgcgcg                                       >  2:4860935/1‑150 (MQ=255)

                                                                                                            tGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAAcgcgcg                                       <  2:4173301/150‑1 (MQ=255)

                                                                                                             gCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCa                                             <  1:4032880/143‑1 (MQ=255)

                                                                                                             gCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCa                                             >  2:2253135/1‑143 (MQ=255)

                                                                                                             gCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCa                                             <  1:2253135/143‑1 (MQ=255)

                                                                                                              cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                                     <  2:2173986/150‑1 (MQ=255)

                                                                                                              cccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGt                                     <  2:5189510/150‑1 (MQ=255)

                                                                                                               ccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGACCGCGCGGTg                                    >  2:626696/1‑150 (MQ=255)

                                                                                                                cTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGcc                                                                               <  1:702416/106‑1 (MQ=255)

                                                                                                                      gATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCt                             >  1:809932/1‑150 (MQ=255)

                                                                                                                          cccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTgg                         <  1:5171402/150‑1 (MQ=255)

                                                                                                                             tgtgcaccGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtg                      >  1:732237/7‑149 (MQ=255)

                                                                                                                               gCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCtt                    <  1:648363/150‑1 (MQ=255)

                                                                                                                               gCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCtt                    <  2:1841496/150‑1 (MQ=255)

                                                                                                                                gcaccGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtgcgg                   <  1:1392087/147‑5 (MQ=255)

                                                                                                                                cTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTg                   <  1:1895048/150‑1 (MQ=255)

                                                                                                                                 tGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGa                  >  1:3386475/1‑150 (MQ=255)

                                                                                                                                  gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                 <  1:3633068/150‑1 (MQ=255)

                                                                                                                                  gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                 <  1:896111/150‑1 (MQ=255)

                                                                                                                                  accGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtgcggaa                 >  2:2343858/2‑144 (MQ=255)

                                                                                                                                  accGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtgcggaa                 >  2:5037294/2‑144 (MQ=255)

                                                                                                                                     gATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAacac              >  2:2445873/1‑150 (MQ=255)

                                                                                                                                       tGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAacacac            >  2:4255213/1‑150 (MQ=255)

                                                                                                                                           gATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgac        <  1:3866009/150‑1 (MQ=255)

                                                                                                                                           gATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgac        >  1:4911098/1‑150 (MQ=255)

                                                                                                                                           gATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCGGTGGGTCTTGAACACaccgac        >  2:299620/1‑150 (MQ=255)

                                                                                                                                            aTCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                                                                                               >  1:1067331/1‑62 (MQ=255)

                                                                                                                                            aTCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgacc       >  1:3130281/1‑150 (MQ=255)

                                                                                                                                            aTCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgacc       >  1:3640343/1‑150 (MQ=255)

                                                                                                                                             tCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCGTGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgaccg      <  2:757615/150‑1 (MQ=255)

                                                                                                                                               cAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGc    <  2:3917516/150‑1 (MQ=255)

                                                                                                                                                aGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCt   >  2:3780721/1‑150 (MQ=255)

CCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTT  >  NC_002947/5738871‑5739165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`