BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,739,145	C→G	6.4%	K93N (AAG→AAC) ‡	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,739,145	0	C	G	6.4%	562.3 / 21.7	171	K93N (AAG→AAC) ‡	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base C (91/69); new base G (7/4); total (98/73)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 176 total aligned reads displayed.

TGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTC  >  NC_002947/5738996‑5739294

  gCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCtt                                                                                                                                                     <  1:648363/150‑1 (MQ=255)

   cTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTg                                                                                                                                                    <  1:1895048/150‑1 (MQ=255)

     gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                                                                                                  <  1:895972/150‑1 (MQ=255)

     gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                                                                                                  <  1:896111/150‑1 (MQ=255)

        gATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAacac                                                                                                                                               >  2:2445873/1‑150 (MQ=255)

          tGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAacacac                                                                                                                                             >  2:4255213/1‑150 (MQ=255)

              gATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgac                                                                                                                                         <  1:3866009/150‑1 (MQ=255)

              gATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgac                                                                                                                                         >  2:299327/1‑150 (MQ=255)

               aTCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgacc                                                                                                                                        >  1:3640343/1‑150 (MQ=255)

                tCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCGTGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgaccg                                                                                                                                       <  2:757615/150‑1 (MQ=255)

                 ccAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCgg                                                                                                                                      >  1:1170343/1‑150 (MQ=255)

                  cAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGc                                                                                                                                     <  2:3917516/150‑1 (MQ=255)

                   aGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCt                                                                                                                                    >  2:3780721/1‑150 (MQ=255)

                   aGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCt                                                                                                                                    >  1:1731115/1‑150 (MQ=255)

                    gCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCtt                                                                                                                                   <  2:897714/150‑1 (MQ=255)

                        ccGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGccc                                                                                                                               <  2:2811767/150‑1 (MQ=255)

                          ggcggTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGa                                                                                                                             >  2:1353906/1‑150 (MQ=255)

                               tCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCa                                                                                                                        <  1:4860935/150‑1 (MQ=255)

                                ggccTGGCGATGTATGGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCAc                                                                                                                       >  2:2069839/4‑150 (MQ=255)

                                ggccTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCAc                                                                                                                       >  1:3831481/4‑150 (MQ=255)

                                 gccTGGCGATGTATGGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCAcc                                                                                                                      >  1:4138971/3‑150 (MQ=255)

                                    tGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACcagc                                                                                                                   >  2:3478572/1‑150 (MQ=255)

                                      gCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACcagcag                                                                                                                 >  1:5303194/1‑150 (MQ=255)

                                       ccATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACcagcaag                                                                                                                >  2:1724442/1‑148 (MQ=255)

                                       ccATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                <  2:4303909/150‑1 (MQ=255)

                                        cATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGAGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAggg                                                                                                               >  2:984874/1‑150 (MQ=255)

                                        cATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAAGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAggg                                                                                                               <  2:4855892/150‑1 (MQ=255)

                                         aTACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGt                                                                                                              >  1:2037452/1‑150 (MQ=255)

                                         aTACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGt                                                                                                              >  1:2037436/1‑150 (MQ=255)

                                          tACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTc                                                                                                                                                       >  1:3861139/1‑108 (MQ=255)

                                          tACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTc                                                                                                                                                       <  2:3861139/108‑1 (MQ=255)

                                           gtatggCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGttt                                                                                                            >  2:3115074/6‑150 (MQ=255)

                                           aCATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGttt                                                                                                            >  2:382347/1‑150 (MQ=255)

                                              tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGTTCACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgt                                                                                                         >  2:2060353/1‑150 (MQ=255)

                                              tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                <  2:3087736/143‑1 (MQ=255)

                                              tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                >  1:3087736/1‑143 (MQ=255)

                                              tCGCCAGGCCCTTGGCGACCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcga                                                                                                         >  2:3309363/1‑149 (MQ=255)

                                               cGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgtc                                                                                                        <  1:5031752/150‑1 (MQ=255)

                                               cGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgtc                                                                                                        <  1:4275491/150‑1 (MQ=255)

                                                  cAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCgtcgtc                                                                                                     <  2:2890039/150‑1 (MQ=255)

                                                    ggCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGGTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCtt                                                                                                   >  2:2681609/1‑150 (MQ=255)

                                                       ccTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCa                                                                                                >  1:5205075/1‑150 (MQ=255)

                                                            gCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                                                                                           <  2:2231196/150‑1 (MQ=255)

                                                              gTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGt                                                                                         <  1:4295256/150‑1 (MQ=255)

                                                                ccAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAacaca                                                                                                                                              >  2:1899852/1‑95 (MQ=255)

                                                                  aGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAgg                                                                                     <  1:1573876/150‑1 (MQ=255)

                                                                    ttCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGt                                                                                   >  1:5359378/1‑150 (MQ=255)

                                                                         tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                                                                              <  1:3798069/150‑1 (MQ=255)

                                                                         tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                                                                              <  1:3115074/150‑1 (MQ=255)

                                                                         tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                                                                              >  1:4958575/1‑150 (MQ=255)

                                                                          gAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcgac                                                                             <  1:1010360/150‑1 (MQ=255)

                                                                           aaGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcgacg                                                                            >  2:4489980/1‑150 (MQ=255)

                                                                            aGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                <  1:2576207/113‑1 (MQ=255)

                                                                            aGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                >  2:2576207/1‑113 (MQ=255)

                                                                            aGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcgacga                                                                           >  1:4942914/1‑150 (MQ=255)

                                                                              tgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATc                                                                         <  2:3552126/150‑1 (MQ=255)

                                                                                ttCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCtt                                                                       >  1:4049522/1‑150 (MQ=255)

                                                                                 tCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTg                                                                      <  1:3477750/150‑1 (MQ=255)

                                                                                      gTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTa                                                                 >  1:2454671/1‑150 (MQ=255)

                                                                                        tGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGc                                                               <  1:3016421/150‑1 (MQ=255)

                                                                                          gCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTgggc                                                                                                                       <  2:1159887/92‑4 (MQ=255)

                                                                                           cccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAgg                                                            >  2:5306137/1‑150 (MQ=255)

                                                                                           cccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAgg                                                            <  2:2790599/150‑1 (MQ=255)

                                                                                              aGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGccc                                                         <  1:1942991/150‑1 (MQ=255)

                                                                                               gtgtGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCa                                                        >  1:2455364/1‑150 (MQ=255)

                                                                                               gtgtGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCa                                                        <  1:1064793/150‑1 (MQ=255)

                                                                                                tgtgGCAACAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCCGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAg                                                       >  2:2114368/1‑150 (MQ=255)

                                                                                                 gtgGCACCAGGGTGGAGTTGGCAATCAGCAGGCGCGGTGCGTTGCTGTGGGTCGGGATCACACCGAGCGGCTTGCCCGACTGCTCCAGCAGGGGTTCGTCGTCTTGCAGGCGGGTCCGGGTTTCGACGTTCTTGTCGTTGCTggagatcg                                                      >  2:239079/1‑144 (MQ=255)

                                                                                                  tgGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGtt                                                     <  2:1058861/150‑1 (MQ=255)

                                                                                                     cACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcg                                                  >  1:1054938/1‑150 (MQ=255)

                                                                                                     aaCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcg                                                  >  2:641214/2‑150 (MQ=255)

                                                                                                      aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                                                                                           <  2:4078648/108‑1 (MQ=255)

                                                                                                      aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                                                                                           >  1:4078812/1‑108 (MQ=255)

                                                                                                      aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                                                                                           >  1:4078648/1‑108 (MQ=255)

                                                                                                      aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcgc                                                 >  1:2305773/1‑150 (MQ=255)

                                                                                                        cAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcgcgc                                               >  1:1308294/1‑150 (MQ=255)

                                                                                                           gTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGATGCgcgcggc                                            >  2:690688/1‑150 (MQ=255)

                                                                                                            ttGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCgcgcggcg                                           >  1:3149083/1‑150 (MQ=255)

                                                                                                                  ttGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCg                                     >  1:1677054/1‑150 (MQ=255)

                                                                                                                    ggCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGAt                                   >  2:2403007/1‑150 (MQ=255)

                                                                                                                       gatgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGcc                                <  1:4241487/143‑1 (MQ=255)

                                                                                                                       aaTCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAcc                                >  1:1327963/1‑150 (MQ=255)

                                                                                                                         tgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGccgc                              >  2:3159655/6‑150 (MQ=255)

                                                                                                                           aGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAccgccg                            >  2:5111034/1‑150 (MQ=255)

                                                                                                                           aGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAccgccg                            >  2:2382366/1‑150 (MQ=255)

                                                                                                                            gCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGt                           >  2:1384270/1‑150 (MQ=255)

                                                                                                                            gCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGt                           >  2:4131899/1‑150 (MQ=255)

                                                                                                                             cACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGta                          >  1:3690727/1‑150 (MQ=255)

                                                                                                                             cACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGta                          <  1:4552244/150‑1 (MQ=255)

                                                                                                                              aCGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGtat                         >  1:2012599/1‑150 (MQ=255)

                                                                                                                                gcgcgGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGac                       >  1:3741416/1‑150 (MQ=255)

                                                                                                                                    ggTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATaccacc                   >  1:692764/1‑150 (MQ=255)

                                                                                                                                     gTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATAccacca                  <  2:3386475/150‑1 (MQ=255)

                                                                                                                                      tGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                                                                    <  1:220443/99‑1 (MQ=255)

                                                                                                                                      tGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAg                 >  2:471536/1‑150 (MQ=255)

                                                                                                                                          ttGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGctct             <  2:3747166/150‑1 (MQ=255)

                                                                                                                                             cTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTc          <  1:29619/150‑1 (MQ=255)

                                                                                                                                               gtgGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCgg        <  2:4692110/150‑1 (MQ=255)

                                                                                                                                                tgGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCggg       <  2:2846065/150‑1 (MQ=255)

                                                                                                                                                     cTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTc  <  1:5296746/150‑1 (MQ=255)

TGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTC  >  NC_002947/5738996‑5739294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`