BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,739,183	G→A	5.2%	L81L (CTG→TTG)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,739,183	0	G	A	5.2%	537.9 / 12.1	171	L81L (CTG→TTG)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base G (90/72); new base A (6/3); total (96/75)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 190 total aligned reads displayed.

GCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGC  >  NC_002947/5739034‑5739331

 ccATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                                                     <  2:4188475/150‑1 (MQ=255)

  cATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGAGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAggg                                                                                                                                                    >  2:984874/1‑150 (MQ=255)

   aTACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGt                                                                                                                                                   >  1:2037452/1‑150 (MQ=255)

     gtatggCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGttt                                                                                                                                                 >  2:3115074/6‑150 (MQ=255)

     aCATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGttt                                                                                                                                                 >  2:382347/1‑150 (MQ=255)

       aTCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcg                                                                                                                                               >  2:2368139/1‑150 (MQ=255)

        tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                                                     <  2:3087736/143‑1 (MQ=255)

        tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                                                                                                                     >  1:3087736/1‑143 (MQ=255)

         cGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgtc                                                                                                                                             <  1:4275491/150‑1 (MQ=255)

           ccAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcg                                                                                                                                               >  2:2963993/1‑146 (MQ=255)

           ccAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcg                                                                                                                                               <  1:2963993/146‑1 (MQ=255)

            cAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCgtcgtc                                                                                                                                          <  2:2890039/150‑1 (MQ=255)

                   ttGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAgg                                                                                                                                   >  2:4372988/1‑150 (MQ=255)

                        gTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGt                                                                                                                              <  1:140532/150‑1 (MQ=255)

                           cAGTTCGTTGACGTGGTCCCAGTTGGCCCAGTGTGGCACCAGGCTGGAGTTGGCAATCAGCACGCGCGGTGCGCTGCTGTGGGTCTTGAACCCACCGACCGGCTTGCCCGACGGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAg                                                                                                                           <  2:5394533/150‑1 (MQ=255)

                                   tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                                                                                                                   <  1:3115074/150‑1 (MQ=255)

                                    gAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcgac                                                                                                                  <  1:1010360/150‑1 (MQ=255)

                                      aGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcgacga                                                                                                                >  1:4942914/1‑150 (MQ=255)

                                       gtgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGAt                                                                                                               >  2:3425881/1‑150 (MQ=255)

                                        tgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATc                                                                                                              <  2:3552126/150‑1 (MQ=255)

                                          ttCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCtt                                                                                                            >  1:4049522/1‑150 (MQ=255)

                                           tCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTg                                                                                                           <  1:2500683/150‑1 (MQ=255)

                                           tCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTg                                                                                                           <  1:3477750/150‑1 (MQ=255)

                                             ccAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                                                                                                         >  1:4469106/1‑150 (MQ=255)

                                                gTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTa                                                                                                      >  1:2454671/1‑150 (MQ=255)

                                                gTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTa                                                                                                      >  1:927735/1‑150 (MQ=255)

                                                  tGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGc                                                                                                    <  1:3016421/150‑1 (MQ=255)

                                                    cgatctggtgGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCCTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAg                                                                                                  <  1:239079/143‑1 (MQ=255)

                                                     cccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAgg                                                                                                 <  2:2790599/150‑1 (MQ=255)

                                                         gtgtGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCa                                                                                             <  1:1064793/150‑1 (MQ=255)

                                                            tgGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGtt                                                                                          <  2:1058861/150‑1 (MQ=255)

                                                               cACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcg                                                                                       >  1:1054938/1‑150 (MQ=255)

                                                                aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                                                                                                                                >  1:4078648/1‑108 (MQ=255)

                                                                aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                                                                                                                                <  2:4078648/108‑1 (MQ=255)

                                                                aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGATGTGGGTCTTGAACACACCGACCGCCTTGCCAGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcgc                                                                                      >  2:3984469/1‑150 (MQ=255)

                                                                  cAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcgcgc                                                                                    >  1:1308294/1‑150 (MQ=255)

                                                                     gTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCgcgcggc                                                                                 >  2:3367344/1‑150 (MQ=255)

                                                                     gTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCgcgcggc                                                                                 >  2:2091504/1‑150 (MQ=255)

                                                                     gTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGATGCgcgcggc                                                                                 >  2:690688/1‑150 (MQ=255)

                                                                           gTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                                                                                                         <  1:4980410/120‑1 (MQ=255)

                                                                            ttGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCg                                                                          >  1:1677054/1‑150 (MQ=255)

                                                                              ggCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGAt                                                                        >  2:2403006/1‑150 (MQ=255)

                                                                                 gatgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGcc                                                                     <  1:4241487/143‑1 (MQ=255)

                                                                                 aaTCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGCTAcc                                                                     <  1:5306137/150‑1 (MQ=255)

                                                                                 aaTCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAcc                                                                     >  1:3712748/1‑150 (MQ=255)

                                                                                   tgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGccgc                                                                   >  2:3159655/6‑150 (MQ=255)

                                                                                     aGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAccgccg                                                                 >  2:2382366/1‑150 (MQ=255)

                                                                                      gCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGt                                                                >  2:4131899/1‑150 (MQ=255)

                                                                                      gCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGt                                                                >  2:1384270/1‑150 (MQ=255)

                                                                                       cACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGta                                                               <  1:4552244/150‑1 (MQ=255)

                                                                                       cACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGta                                                               >  2:2489470/1‑150 (MQ=255)

                                                                                        aCGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGtat                                                              >  1:2012599/1‑150 (MQ=255)

                                                                                        aCGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGtat                                                              >  1:4837868/1‑150 (MQ=255)

                                                                                              ggTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATaccacc                                                        >  1:692761/1‑150 (MQ=255)

                                                                                                tGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                                                                                                         >  2:220443/1‑99 (MQ=255)

                                                                                                tGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                                                                                                         <  1:220443/99‑1 (MQ=255)

                                                                                                tGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAg                                                      >  2:471536/1‑150 (MQ=255)

                                                                                                   gTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGctc                                                   >  1:2101368/1‑150 (MQ=255)

                                                                                                     tGCTGTGTGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCtt                                                 <  2:283650/150‑1 (MQ=255)

                                                                                                          tgGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCggg                                            <  1:4123985/150‑1 (MQ=255)

                                                                                                            ggTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCgg                                             <  2:859522/147‑1 (MQ=255)

                                                                                                              tCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGtttt                                        <  2:1170343/150‑1 (MQ=255)

                                                                                                               cTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTc                                       <  1:382347/150‑1 (MQ=255)

                                                                                                               cTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTc                                       <  1:5296746/150‑1 (MQ=255)

                                                                                                                ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                      >  2:2585025/1‑150 (MQ=255)

                                                                                                                ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                      >  1:4066515/1‑150 (MQ=255)

                                                                                                                ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                      >  2:931232/1‑150 (MQ=255)

                                                                                                                 tGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCcg                                     <  1:1896529/150‑1 (MQ=255)

                                                                                                                  gAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCcgc                                    <  2:5205075/150‑1 (MQ=255)

                                                                                                                    acacacCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGa                                  >  1:4577933/1‑150 (MQ=255)

                                                                                                                      acacCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGAcc                                >  1:749829/1‑150 (MQ=255)

                                                                                                                        accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                              >  2:516679/1‑150 (MQ=255)

                                                                                                                        accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                              >  2:3027145/1‑150 (MQ=255)

                                                                                                                        accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                              >  2:2973623/1‑150 (MQ=255)

                                                                                                                          cgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtgtg                            <  2:2398130/150‑1 (MQ=255)

                                                                                                                           caccgGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGAcatccgg                           <  2:3741416/149‑7 (MQ=255)

                                                                                                                            accgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGa                          >  1:862920/1‑150 (MQ=255)

                                                                                                                            accgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGa                          <  1:2368139/150‑1 (MQ=255)

                                                                                                                                 cTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAg                     >  1:5215716/1‑150 (MQ=255)

                                                                                                                                 cTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGACCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAg                     <  2:5303194/150‑1 (MQ=255)

                                                                                                                                     cccGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgttg                 <  2:2012599/150‑1 (MQ=255)

                                                                                                                                      ccGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                      >  1:848535/1‑128 (MQ=255)

                                                                                                                                      ccGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgttgt                >  2:5125830/1‑150 (MQ=255)

                                                                                                                                      ccGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgttgt                >  1:1834032/1‑150 (MQ=255)

                                                                                                                                       cGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgttgtt               >  2:4682867/1‑150 (MQ=255)

                                                                                                                                        gACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtc              <  2:3910961/150‑1 (MQ=255)

                                                                                                                                        gACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtc              <  1:2585025/150‑1 (MQ=255)

                                                                                                                                          cTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtcat            <  2:4911098/150‑1 (MQ=255)

                                                                                                                                           tGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtcatc           <  2:809932/150‑1 (MQ=255)

                                                                                                                                           tGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtcatc           <  2:2454671/150‑1 (MQ=255)

                                                                                                                                             cACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAg         >  1:1944943/1‑150 (MQ=255)

                                                                                                                                             cACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAg         <  1:299327/150‑1 (MQ=255)

                                                                                                                                              aCCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGc        >  1:2299302/1‑150 (MQ=255)

                                                                                                                                              aCCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGc        <  1:3795185/150‑1 (MQ=255)

                                                                                                                                               ccAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTAGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCa       >  2:2412921/1‑150 (MQ=255)

                                                                                                                                                 agcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAg                     <  1:5038664/134‑1 (MQ=255)

                                                                                                                                                 agcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAg                     >  2:5038664/1‑134 (MQ=255)

                                                                                                                                                 agcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATg     >  1:4628370/1‑150 (MQ=255)

                                                                                                                                                  gcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATgc    >  1:4390355/1‑150 (MQ=255)

                                                                                                                                                    agGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATgcgc  >  1:2045403/1‑150 (MQ=255)

GCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGC  >  NC_002947/5739034‑5739331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`