BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	433,877	G→T	6.8%	S442S (TCC→TCA) ‡	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	433,877	0	G	T	6.8%	723.6 / 29.5	219	S442S (TCC→TCA) ‡	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base G (110/94); new base T (10/5); total (120/99)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 222 total aligned reads displayed.

TTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGAT  >  NC_002947/433728‑434026

ttttCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAg                                                                                                                                                       <  1:1283181/150‑1 (MQ=255)

       aGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                                                <  2:1274205/150‑1 (MQ=255)

       aGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                                                <  2:1120886/150‑1 (MQ=255)

          cgcCGATCCACGTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGt                                                                                                                                             <  2:4265317/150‑1 (MQ=255)

           gcCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                                                >  1:1001194/1‑146 (MQ=255)

           gcCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                                                <  2:1001194/146‑1 (MQ=255)

           gcCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGtt                                                                                                                                            >  1:2099308/1‑150 (MQ=255)

                   aCTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTgg                                                                                                                                    >  2:1792974/1‑150 (MQ=255)

                   aCTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTgg                                                                                                                                    >  2:5251049/1‑150 (MQ=255)

                       ctcGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACg                                                                                                                                >  2:401808/1‑150 (MQ=255)

                        tcGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACgg                                                                                                                               >  1:1664916/1‑150 (MQ=255)

                        tcGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACgg                                                                                                                               >  2:3634091/1‑150 (MQ=255)

                           ttcttcATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGa                                                                                                                                          <  1:1381307/136‑1 (MQ=255)

                           ttcttcATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGa                                                                                                                                          >  2:1381307/1‑136 (MQ=255)

                           ttattcATGGCACCTGTGCGCACCACGGCGCCGGCTTCCGGCGAGGTGTGGCTTTCGTCGGCAGTGAGGTCAAGGAGGCCGGTGGGGGTGAACACCACGCGCTCGGCGGCGGCCTTGGTGGAGGACGTGAGGTGGGCCGGGGTGGGGCGt                                                                                                                            >  2:4453984/4‑150 (MQ=255)

                               tcATGTCGCCCTTGCGGATCATCGGGCCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCt                                                                                                                        >  2:4672319/1‑150 (MQ=255)

                                cATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTc                                                                                                                       >  1:2964071/1‑150 (MQ=255)

                                   ggCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTc                                                                                                                    <  1:1811072/150‑1 (MQ=255)

                                   ggCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTc                                                                                                                    >  2:847357/1‑150 (MQ=255)

                                    gCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTcc                                                                                                                   <  2:5243634/150‑1 (MQ=255)

                                      aCCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCat                                                                                                                 >  2:1931543/1‑150 (MQ=255)

                                      aCCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCat                                                                                                                 <  1:2663000/150‑1 (MQ=255)

                                        cTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCatga                                                                                                               <  1:5400323/150‑1 (MQ=255)

                                        cTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCatga                                                                                                               >  1:447917/1‑150 (MQ=255)

                                         tttGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCatgat                                                                                                              >  2:4944688/1‑150 (MQ=255)

                                            gcgcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGcc                                                                                                           <  2:1653539/150‑1 (MQ=255)

                                            gcgcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGcc                                                                                                           <  1:4944659/150‑1 (MQ=255)

                                              gcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGa                                                                                                         >  2:2781008/1‑150 (MQ=255)

                                              gcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGa                                                                                                         >  2:1118171/1‑150 (MQ=255)

                                              gcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGa                                                                                                         >  2:3595543/1‑150 (MQ=255)

                                              gcACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGa                                                                                                         <  1:4307324/150‑1 (MQ=255)

                                                accacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGAcc                                                                                                       >  1:4087711/1‑150 (MQ=255)

                                                      gcgcCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACg                                                                                                 <  2:4161530/150‑1 (MQ=255)

                                                       cgcCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACgg                                                                                                <  1:2028319/150‑1 (MQ=255)

                                                        gcCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTc                                                                                                                          <  1:4385899/123‑1 (MQ=255)

                                                        gcCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTc                                                                                                                          >  2:4385899/1‑123 (MQ=255)

                                                           ggCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGtt                                                                                            <  2:944505/150‑1 (MQ=255)

                                                             cTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTgc                                                                                          <  1:5060013/150‑1 (MQ=255)

                                                              ttCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGTCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTgcg                                                                                         >  1:382709/1‑150 (MQ=255)

                                                               tCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTgcgc                                                                                        <  2:1501567/150‑1 (MQ=255)

                                                                 cATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCgg                                                                                      <  2:611512/150‑1 (MQ=255)

                                                                 cATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCgg                                                                                      >  1:2804632/1‑150 (MQ=255)

                                                                 cATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCgg                                                                                      >  1:3625430/1‑150 (MQ=255)

                                                                  aTCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGc                                                                                     >  2:2776907/1‑150 (MQ=255)

                                                                     gAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATg                                                                                  >  2:2120797/1‑150 (MQ=255)

                                                                       ggTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGcc                                                                                <  1:3935138/150‑1 (MQ=255)

                                                                        gtgtGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGcc                                                                                >  2:533572/1‑149 (MQ=255)

                                                                        gtgtGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGcc                                                                                <  1:533678/149‑1 (MQ=255)

                                                                         tgtgGATTTCATCGCCAGTGCGGTCAAGGTAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGTGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCca                                                                              >  2:385502/1‑150 (MQ=255)

                                                                          gtgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcag                                                                             >  2:2492352/1‑150 (MQ=255)

                                                                               tttCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGt                                                                        >  2:5433183/1‑150 (MQ=255)

                                                                                ttCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTc                                                                                                                          >  1:3288647/1‑99 (MQ=255)

                                                                                 tCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCt                                                                      >  1:1189535/1‑150 (MQ=255)

                                                                                   aTCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTc                                                                    <  2:4920013/150‑1 (MQ=255)

                                                                                    tCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCg                                                                   >  1:545625/1‑150 (MQ=255)

                                                                                      gCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGAc                                                                 <  1:481001/150‑1 (MQ=255)

                                                                                      gCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCggt                                                                 >  2:2345917/1‑148 (MQ=255)

                                                                                       ccAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCggtg                                                                >  1:1863008/1‑147 (MQ=255)

                                                                                        cAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCggtgc                                                               >  1:4218319/1‑146 (MQ=255)

                                                                                          gTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                                                             <  1:1469478/150‑1 (MQ=255)

                                                                                            gCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCt                                                           <  1:1451827/150‑1 (MQ=255)

                                                                                            gCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCt                                                           <  1:3478712/150‑1 (MQ=255)

                                                                                              ggTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGa                                                         <  1:1234482/150‑1 (MQ=255)

                                                                                               gTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGaa                                                        <  1:261628/150‑1 (MQ=255)

                                                                                                tCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAg                                                       >  2:5343243/1‑150 (MQ=255)

                                                                                                tCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAg                                                       >  1:4128625/1‑150 (MQ=255)

                                                                                                 cAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGCCTTCGACCTGGCTGAAGa                                                      <  1:1120062/150‑1 (MQ=255)

                                                                                                  aaGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCatgat                                                                                                              >  1:3813256/1‑93 (MQ=255)

                                                                                                  aaGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAt                                                     <  1:89473/150‑1 (MQ=255)

                                                                                                    ggAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtct                                                   >  2:796833/1‑150 (MQ=255)

                                                                                                     gAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtctc                                                  >  1:2410380/1‑150 (MQ=255)

                                                                                                         ccGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTg                                              <  1:4970501/150‑1 (MQ=255)

                                                                                                          cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACg                                                                                                 >  2:1768360/1‑98 (MQ=255)

                                                                                                          cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACg                                                                                                 <  1:1768360/98‑1 (MQ=255)

                                                                                                          cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTgg                                             <  2:4762062/150‑1 (MQ=255)

                                                                                                           ggTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGc                                            <  1:4004567/150‑1 (MQ=255)

                                                                                                            gtgtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCg                                           >  2:5007949/1‑150 (MQ=255)

                                                                                                             tgtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGACGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGa                                          >  1:324103/1‑150 (MQ=255)

                                                                                                                 gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                                      <  1:1058041/150‑1 (MQ=255)

                                                                                                                 gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                                      >  2:3656827/1‑150 (MQ=255)

                                                                                                                 gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                                      <  1:3064858/150‑1 (MQ=255)

                                                                                                                 gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                                      >  2:2810369/1‑150 (MQ=255)

                                                                                                                  atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGcc                                     <  1:4606711/150‑1 (MQ=255)

                                                                                                                   tgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCa                                    >  2:1523162/1‑150 (MQ=255)

                                                                                                                   tgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCa                                    >  1:5280573/1‑150 (MQ=255)

                                                                                                                       caccacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTc                                <  2:3191725/150‑1 (MQ=255)

                                                                                                                          cacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTc                             >  2:3204296/1‑150 (MQ=255)

                                                                                                                          cacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTc                             >  1:2554236/1‑150 (MQ=255)

                                                                                                                           acGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCa                            <  1:3877409/150‑1 (MQ=255)

                                                                                                                             gcgcTCGGCGGCTGCCTTGATTCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCATCAGGTCTTCGTCCTGTCTTATGATCTCGGTGGCGAAGGCCTCTTCCTctgt                          <  2:4942565/150‑4 (MQ=255)

                                                                                                                             gcgcTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAgg                          >  1:2292340/1‑150 (MQ=255)

                                                                                                                                cTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGcc                       <  1:249233/150‑1 (MQ=255)

                                                                                                                                 tCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCg                      >  2:1828339/1‑150 (MQ=255)

                                                                                                                                 tCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCg                      >  2:3953737/1‑150 (MQ=255)

                                                                                                                                 tCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCg                      >  1:1254872/1‑150 (MQ=255)

                                                                                                                                  cggcggCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGt                     <  2:2716422/150‑1 (MQ=255)

                                                                                                                                         tGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGtt                                                                                            >  2:2388967/1‑72 (MQ=255)

                                                                                                                                         tGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCtt              >  1:40413/1‑150 (MQ=255)

                                                                                                                                                   cAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACg    >  1:705550/1‑150 (MQ=255)

                                                                                                                                                     ggACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGAt  <  2:2956846/150‑1 (MQ=255)

TTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGAT  >  NC_002947/433728‑434026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`