Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,755,463 T→G 11.6% intergenic (+46/‑320) glnA → / → glnL glutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,755,4630TG11.6% 483.0 / 45.9 171intergenic (+46/‑320)glnA/glnLglutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG
Reads supporting (aligned to +/- strand):  ref base T (65/85);  new base G (12/8);  total (78/93)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 185 total aligned reads displayed.

CCGACGACTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTCTG  >  NC_002947/5755314‑5755611
                                                                                                                                                     |                                                                                                                                                    
ccGACGACTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGcctccct                                                                                                                                                      <  1:307734/150‑1 (MQ=255)
 cgacgaCTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCGAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGcctccctc                                                                                                                                                     >  2:779971/1‑150 (MQ=255)
  gacgacTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCg                                                                                                                                                    >  1:2738498/1‑150 (MQ=255)
     gacTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGa                                                                                                                                                 <  1:3019929/150‑1 (MQ=255)
     gacTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGa                                                                                                                                                 <  2:1555015/150‑1 (MQ=255)
      acTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAg                                                                                                                                                <  1:2960207/150‑1 (MQ=255)
       cTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCgagggagg                                                                                                                                               <  1:2376840/150‑1 (MQ=255)
        ttCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGc                                                                                                                                              >  1:4569484/1‑150 (MQ=255)
        ttCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGCGGGAGGc                                                                                                                                              >  1:3309256/1‑150 (MQ=255)
           aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGt                                                                                                                                           >  1:413937/1‑150 (MQ=255)
           aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGt                                                                                                                                           >  2:3234128/1‑150 (MQ=255)
            tCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGtt                                                                                                                                          <  1:5252896/150‑1 (MQ=255)
            tCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGtt                                                                                                                                          <  2:4263088/150‑1 (MQ=255)
             cGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttt                                                                                                                                         <  1:3317206/150‑1 (MQ=255)
              gATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAATCGGCCTCCCTCGGGAGGCCGtttt                                                                                                                                        >  1:595918/1‑150 (MQ=255)
               aTGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttttt                                                                                                                                       <  2:4646726/150‑1 (MQ=255)
               aTGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttttt                                                                                                                                       <  1:3119099/150‑1 (MQ=255)
               aTGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttttt                                                                                                                                       >  2:2709041/1‑150 (MQ=255)
               aTGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGatttt                                                                                                                                       <  1:5140884/150‑1 (MQ=255)
                tGCCTTCATCGAGCTGAATAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAACCGGCCTCCCGAGGGAGGCCGttttga                                                                                                                                      <  2:2333232/150‑3 (MQ=255)
                tGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGATTTTg                                                                                                                                      <  2:5440792/150‑1 (MQ=255)
                 gCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCTGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGt                                                                                                                                     <  1:3208379/150‑1 (MQ=255)
                   cTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGttt                                                                                                                                   >  1:3840058/1‑150 (MQ=255)
                   cTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGttt                                                                                                                                   >  2:1247761/1‑150 (MQ=255)
                   cTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGGACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGttt                                                                                                                                   <  1:1849040/150‑1 (MQ=255)
                    ttCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTc                                                                                                                                  >  2:1382427/1‑150 (MQ=255)
                    ttCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGttttagcaag                                                                                                                                  >  1:3881753/1‑144 (MQ=255)
                       aTCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGACTCCCTCGGGAGGCCGTTTTTGTTTCTgc                                                                                                                               >  1:1100928/1‑150 (MQ=255)
                            gCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGATTTTGTTTCTGCGCAcc                                                                                                                          <  1:228813/150‑1 (MQ=255)
                               gAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTg                                                                                                                       >  1:945502/1‑150 (MQ=255)
                                  gagCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAg                                                                                                                    <  1:2661733/150‑1 (MQ=255)
                                  gagCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAg                                                                                                                    <  1:1469244/150‑1 (MQ=255)
                                   agCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGa                                                                                                                   <  1:47139/150‑1 (MQ=255)
                                         gaagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGcgcc                                                                                                             >  2:396069/1‑150 (MQ=255)
                                          aagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAATACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGcgccg                                                                                                            >  2:565164/1‑150 (MQ=255)
                                            gaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCt                                                                                                          <  1:1617392/150‑1 (MQ=255)
                                              aaTCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGa                                                                                                        <  2:1670294/150‑1 (MQ=255)
                                               aTCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGAt                                                                                                       >  2:1805532/1‑150 (MQ=255)
                                               aTCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGAt                                                                                                       >  1:1220094/1‑150 (MQ=255)
                                               aTCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCACGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGAGCCGCTGAt                                                                                                       >  1:2550069/1‑150 (MQ=255)
                                                 cAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTc                                                                                                     >  1:809243/1‑150 (MQ=255)
                                                  aaGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCg                                                                                                    >  2:1072536/1‑150 (MQ=255)
                                                    ggTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGcc                                                                                                  <  1:3140002/150‑1 (MQ=255)
                                                      tCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGt                                                                                                                                           <  1:2233193/107‑1 (MQ=255)
                                                       ccGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGt                                                                                               >  1:103045/1‑150 (MQ=255)
                                                             cTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCt                                                                                         >  2:3561201/1‑150 (MQ=255)
                                                              ttCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTa                                                                                        <  1:3530287/150‑1 (MQ=255)
                                                               tCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTAt                                                                                       >  1:746805/1‑150 (MQ=255)
                                                                 gTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGc                                                                                     >  2:921429/1‑150 (MQ=255)
                                                                   acacCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAACCGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGctc                                                                                   <  1:2014551/150‑1 (MQ=255)
                                                                   acacCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGATTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGctc                                                                                   <  2:342746/150‑1 (MQ=255)
                                                                     acCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTa                                                                                 <  2:1220094/150‑1 (MQ=255)
                                                                     aaccGCTGGTATTCGAGCTGTACTACTGCTGCTGATATGATTGGCTCCTCGCGCGGATGACTTGATCAATACGGCATCCCTCTGTTGGCCGTTTTTGTTTGTTCGAACCTTGCAGAAGCGCCGCTGATTCGCATGTGACGATATTCTCTa                                                                                 <  2:2550069/148‑1 (MQ=255)
                                                                      cccGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCGGTCACGCTATGCTCTAt                                                                                >  1:5066068/1‑150 (MQ=255)
                                                                        cGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCt                                                                              <  1:3248163/150‑1 (MQ=255)
                                                                         gCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCtt                                                                             <  1:717100/150‑1 (MQ=255)
                                                                          cTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTg                                                                            <  1:4348254/150‑1 (MQ=255)
                                                                            ggAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGt                                                                          >  2:2704127/1‑150 (MQ=255)
                                                                             gAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTg                                                                         >  2:4469798/1‑150 (MQ=255)
                                                                                  cGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTa                                                                    <  1:404532/150‑1 (MQ=255)
                                                                                   gAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTaa                                                                   >  1:4698183/1‑150 (MQ=255)
                                                                                                gctgctGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGcc                                                      >  2:654142/1‑150 (MQ=255)
                                                                                                   gctgATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTc                                                   <  2:4002525/150‑1 (MQ=255)
                                                                                                   gctgATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGACCTc                                                   >  2:4748981/1‑150 (MQ=255)
                                                                                                     tgatctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCaa                                                 >  1:4441005/1‑150 (MQ=255)
                                                                                                        tctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTg                                              <  1:1830895/150‑1 (MQ=255)
                                                                                                          tgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCt                                            <  2:4794308/150‑1 (MQ=255)
                                                                                                            atTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTcc                                          <  2:3840058/150‑1 (MQ=255)
                                                                                                            atTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTcc                                          >  2:528650/1‑150 (MQ=255)
                                                                                                             ttGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTccc                                         >  2:1988309/1‑150 (MQ=255)
                                                                                                              tGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTcccc                                        >  2:2357001/1‑150 (MQ=255)
                                                                                                                gcgcCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGtt                                                                                                                                          >  1:3155943/1‑50 (MQ=255)
                                                                                                                     tCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTgg                                 <  2:5145951/150‑1 (MQ=255)
                                                                                                                     tCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTgg                                 >  2:2825058/1‑150 (MQ=255)
                                                                                                                     tCGCGCCGAGGACATGATCAAATCGGCCTCCCTCGGGAGGCCGTTTTTGGTGCTGCGCACCGTGCAGAAGCGCCGCTGATTCGCCGGTCACGCTATGCTCTATCTGGGTGCATTAATTCACACCATGCCCTCAAGTGCTCCCCAATCTgg                                 >  2:2932085/1‑150 (MQ=255)
                                                                                                                       gcgcCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                               <  2:297510/150‑1 (MQ=255)
                                                                                                                         gcCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCa                             <  1:3395795/150‑1 (MQ=255)
                                                                                                                          ccGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAg                            <  1:1382427/150‑1 (MQ=255)
                                                                                                                           cGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAgg                           <  1:1247761/150‑1 (MQ=255)
                                                                                                                           cGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAAGTGCTCCCCAATCTGGTTCAgg                           <  2:3305820/150‑1 (MQ=255)
                                                                                                                            gATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGATTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGt                          <  2:2738498/150‑1 (MQ=255)
                                                                                                                             aTGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGtt                         >  1:3928446/1‑150 (MQ=255)
                                                                                                                             aTGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGtt                         <  2:1845238/150‑1 (MQ=255)
                                                                                                                               gACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTaa                       <  2:2149071/150‑1 (MQ=255)
                                                                                                                                 agaaacaaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAAc                     >  2:695922/8‑150 (MQ=255)
                                                                                                                                     acaaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGccc                                                     >  2:5431361/4‑114 (MQ=255)
                                                                                                                                     acaaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGccc                                                     <  1:5431361/111‑1 (MQ=255)
                                                                                                                                      tCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTc                <  2:4881983/150‑1 (MQ=255)
                                                                                                                                      tCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTc                >  2:5399817/1‑150 (MQ=255)
                                                                                                                                       aaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCa               >  2:890822/2‑150 (MQ=255)
                                                                                                                                        aaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAAtt                                                                 >  1:652058/1‑99 (MQ=255)
                                                                                                                                          aaCGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAgg                           >  1:455868/1‑135 (MQ=255)
                                                                                                                                          aaCGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAgg                           <  2:455868/135‑1 (MQ=255)
                                                                                                                                          aaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCa            >  2:496542/1‑150 (MQ=255)
                                                                                                                                           aCGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCaa           <  1:2709041/150‑1 (MQ=255)
                                                                                                                                                 tccctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACttt     >  2:1336916/1‑150 (MQ=255)
                                                                                                                                                  ccctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTc    <  1:2825058/150‑1 (MQ=255)
                                                                                                                                                  ccctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTc    <  2:3076110/150‑1 (MQ=255)
                                                                                                                                                   cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                               <  1:4047892/122‑1 (MQ=255)
                                                                                                                                                   cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                               >  2:4047892/1‑122 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGACGACTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTCTG  >  NC_002947/5755314‑5755611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: