Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,851,388 A→G 5.0% I39V (ATC→GTC)  ilvD → dihydroxy‑acid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,851,3880AG 5.0% 708.0 / 15.2 218I39V (ATC→GTC) ilvDdihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base A (109/98);  new base G (9/2);  total (118/100)
Fisher's exact test for biased strand distribution p-value = 6.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 233 total aligned reads displayed.

TATTTGTGACCTGATTAAGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCA  >  NC_002947/5851239‑5851533
                                                                                                                                                     |                                                                                                                                                 
     gtgACCTGATTAAGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCatcgccatcgcc                                                                                                                                              <  2:2168800/150‑1 (MQ=255)
        aCCTGATTAAGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAAc                                                                                                                                           <  1:799406/150‑1 (MQ=255)
         ccTGATTAAGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACt                                                                                                                                          >  2:3954847/1‑150 (MQ=255)
          cTGATTAAGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTc                                                                                                                                         >  1:4999457/1‑150 (MQ=255)
          cTGATTAAGCCGCCAGGAGTGTTTCATGCCAGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTc                                                                                                                                         >  1:2754602/1‑150 (MQ=255)
              ttAAGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTc                                                                                                                                     <  2:4347731/150‑1 (MQ=255)
                aaGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCAc                                                                                                                                   >  2:825894/1‑150 (MQ=255)
                  gccgccAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCaa                                                                                                                                            >  2:1510248/1‑139 (MQ=255)
                  gccgccAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCAccc                                                                                                                                 >  1:2708055/1‑150 (MQ=255)
                   ccgccAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCa                                                                                                                                >  1:1274754/1‑150 (MQ=255)
                    cgccAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAg                                                                                                                               >  2:5263871/1‑150 (MQ=255)
                         ggAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCCAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGt                                                                                                                          <  1:2851046/150‑1 (MQ=255)
                          gAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTa                                                                                                                         >  2:456485/1‑150 (MQ=255)
                          gAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTa                                                                                                                         >  2:2271229/1‑150 (MQ=255)
                                 tCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGcc                                                                                                                  >  1:3166548/1‑150 (MQ=255)
                                  cATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCa                                                                                                                 >  2:4231439/1‑150 (MQ=255)
                                   aTGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAt                                                                                                                >  1:2339596/1‑150 (MQ=255)
                                   aTGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAt                                                                                                                <  2:2407855/150‑1 (MQ=255)
                                    tGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAtg                                                                                                               >  1:4679983/1‑150 (MQ=255)
                                    tGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAtg                                                                                                               <  1:1434146/150‑1 (MQ=255)
                                     gCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAtgt                                                                                                              >  1:1523856/1‑150 (MQ=255)
                                     gCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGACGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAtgt                                                                                                              >  2:4092056/1‑150 (MQ=255)
                                     gCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGACGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAggt                                                                                                              >  2:496351/1‑150 (MQ=255)
                                       cTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGc                                                                                                            >  1:3505098/1‑150 (MQ=255)
                                       cTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGc                                                                                                            >  2:116578/1‑150 (MQ=255)
                                         gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                          <  2:4154148/150‑1 (MQ=255)
                                         gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                          >  1:3306136/1‑150 (MQ=255)
                                         gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                          >  1:3117623/1‑150 (MQ=255)
                                               cGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAg                                                                                                    <  2:2950485/150‑1 (MQ=255)
                                                    cAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCt                                                                                               >  1:584823/1‑150 (MQ=255)
                                                     aaGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                              >  2:5363679/1‑150 (MQ=255)
                                                      aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCGTCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                             >  2:2682551/1‑150 (MQ=255)
                                                      aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                             <  2:1771799/150‑1 (MQ=255)
                                                      aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                             <  1:2960478/150‑1 (MQ=255)
                                                          ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                         >  1:3775550/1‑150 (MQ=255)
                                                          ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                         >  2:4469314/1‑150 (MQ=255)
                                                          ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGAACCTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                         >  1:4178206/1‑150 (MQ=255)
                                                          ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCAACCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                         >  1:1111374/1‑150 (MQ=255)
                                                           tCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAg                                                                                        >  1:4396888/1‑150 (MQ=255)
                                                           tCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAg                                                                                        >  1:4076361/1‑150 (MQ=255)
                                                            ccACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGc                                                                                       <  1:4954650/150‑1 (MQ=255)
                                                            ccACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGACACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGc                                                                                       >  1:646879/1‑150 (MQ=255)
                                                             cACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCt                                                                                      <  2:248759/150‑1 (MQ=255)
                                                              aCCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCCACTCGTTCACCCAGTTCGTACCGGGCCCTGTGCACCTGAAGGACCTGGGCCAGCtg                                                                                     >  1:2087264/1‑150 (MQ=255)
                                                              aCCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCtg                                                                                     >  2:1302480/1‑150 (MQ=255)
                                                                ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                   >  2:5349204/1‑150 (MQ=255)
                                                                ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                   >  2:884198/1‑150 (MQ=255)
                                                                ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                   >  2:775419/1‑150 (MQ=255)
                                                                ccAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCtggt                                                                                   >  2:1021278/1‑150 (MQ=255)
                                                                ccAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCtggt                                                                                   <  2:3609931/150‑1 (MQ=255)
                                                                   aGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGc                                                                                >  1:1374163/1‑150 (MQ=255)
                                                                   aGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGc                                                                                >  1:4511188/1‑150 (MQ=255)
                                                                     gCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAt                                                                                                                >  2:3514319/1‑116 (MQ=255)
                                                                        gCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTcgcg                                                                           >  1:3627836/1‑150 (MQ=255)
                                                                           aCATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                        <  2:3774303/150‑1 (MQ=255)
                                                                             aTGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATc                                                                      <  2:3409641/150‑1 (MQ=255)
                                                                               ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                        <  1:2124133/146‑1 (MQ=255)
                                                                               ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                        >  2:2124061/1‑146 (MQ=255)
                                                                                gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                   <  1:1156370/150‑1 (MQ=255)
                                                                                gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                   >  1:1851697/1‑150 (MQ=255)
                                                                                        ccGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCgg                                                           <  1:2449412/150‑1 (MQ=255)
                                                                                          gTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCAGCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtg                                                         <  1:4611712/150‑1 (MQ=255)
                                                                                              ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCaa                                                                                                                                            <  1:1851347/63‑1 (MQ=255)
                                                                                              ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCaa                                                                                                                                            >  2:1851347/1‑63 (MQ=255)
                                                                                              ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgt                                                     >  2:2124941/1‑150 (MQ=255)
                                                                                              ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgt                                                     <  2:479891/150‑1 (MQ=255)
                                                                                               cTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgtg                                                    <  2:282693/150‑1 (MQ=255)
                                                                                                   ggCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCCTGTGCACCTGAAGGACCTGGGCCCGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCa                                                >  2:3310718/1‑150 (MQ=255)
                                                                                                    gcgcgaCACCGGGATGAAGGACGAAGAATTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCaa                                               <  1:4003592/150‑1 (MQ=255)
                                                                                                      gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                             <  2:461539/150‑1 (MQ=255)
                                                                                                      gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                             <  1:1586938/150‑1 (MQ=255)
                                                                                                         ccACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAAt                                          <  1:5277146/150‑1 (MQ=255)
                                                                                                          cACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAAtt                                         >  2:3825233/1‑150 (MQ=255)
                                                                                                           aCCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTc                                        <  1:4486939/150‑1 (MQ=255)
                                                                                                           aCCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTc                                        <  1:882314/150‑1 (MQ=255)
                                                                                                             cGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGACCGCGCCGGTGGCGTGGCCAAGGAATTCaa                                      <  2:5023156/150‑1 (MQ=255)
                                                                                                             cGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCaa                                      <  2:3018301/150‑1 (MQ=255)
                                                                                                              gggATGAAGGAAGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAac                                     <  1:4383833/150‑1 (MQ=255)
                                                                                                                   gAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                   <  1:4567169/115‑1 (MQ=255)
                                                                                                                   gAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCAt                                >  2:3108021/1‑150 (MQ=255)
                                                                                                                   gAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCAAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                   >  2:4567169/1‑115 (MQ=255)
                                                                                                                         cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGaa                                           >  2:1418590/1‑133 (MQ=255)
                                                                                                                         cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGaa                                           <  1:1418548/133‑1 (MQ=255)
                                                                                                                         cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGt                          <  2:1975859/150‑1 (MQ=255)
                                                                                                                         cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTCCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGt                          <  1:4830544/150‑1 (MQ=255)
                                                                                                                          gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                         >  1:4366195/1‑150 (MQ=255)
                                                                                                                          gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                         >  2:501734/1‑150 (MQ=255)
                                                                                                                          gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                         <  1:2563202/150‑1 (MQ=255)
                                                                                                                               cTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGa                    <  2:4590629/150‑1 (MQ=255)
                                                                                                                                ttCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGAc                   >  2:1269567/1‑150 (MQ=255)
                                                                                                                                   aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                >  2:4424740/1‑150 (MQ=255)
                                                                                                                                   aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                >  2:431698/1‑150 (MQ=255)
                                                                                                                                      aaaCCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc             <  1:1936842/150‑1 (MQ=255)
                                                                                                                                           gATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCAt        >  1:4775321/1‑150 (MQ=255)
                                                                                                                                            atcatcGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATg       <  1:3954847/150‑1 (MQ=255)
                                                                                                                                               atcgccatcgccAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc    <  2:4648330/150‑1 (MQ=255)
                                                                                                                                               atcgccatcgccAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc    <  1:347385/150‑1 (MQ=255)
                                                                                                                                               atcgccatcgccAAATCGTTCCCCCAGTTCGTACCGGGCCATGTGCACCTGCAGGACACGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAATGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc    <  1:746/150‑1 (MQ=255)
                                                                                                                                                tcgccatcgccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGcc   <  2:389708/150‑1 (MQ=255)
                                                                                                                                                 cgccatcaccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCa  >  2:4156069/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TATTTGTGACCTGATTAAGCCGCCAGGAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCA  >  NC_002947/5851239‑5851533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑