Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,851,414 A→G 8.2% V47V (GTA→GTG)  ilvD → dihydroxy‑acid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,851,4140AG 8.2% 741.4 / 36.4 245V47V (GTA→GTG) ilvDdihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base A (109/115);  new base G (18/2);  total (128/117)
Fisher's exact test for biased strand distribution p-value = 2.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 250 total aligned reads displayed.

GAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCC  >  NC_002947/5851265‑5851562
                                                                                                                                                     |                                                                                                                                                    
gAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTa                                                                                                                                                      >  2:2271229/1‑150 (MQ=255)
gAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTa                                                                                                                                                      >  2:456618/1‑150 (MQ=255)
       tCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGcc                                                                                                                                               >  1:3166548/1‑150 (MQ=255)
        cATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCa                                                                                                                                              >  2:4231439/1‑150 (MQ=255)
         aTGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAt                                                                                                                                             >  1:2339511/1‑150 (MQ=255)
          tGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAtg                                                                                                                                            >  1:4679983/1‑150 (MQ=255)
           gCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAtgt                                                                                                                                           >  1:1523856/1‑150 (MQ=255)
           gCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGACGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAggt                                                                                                                                           >  2:496351/1‑150 (MQ=255)
             cTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGc                                                                                                                                         >  2:116578/1‑150 (MQ=255)
             cTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGc                                                                                                                                         >  1:3505098/1‑150 (MQ=255)
              tGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCa                                                                                                                                        <  2:3695354/150‑1 (MQ=255)
               gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                                                       <  2:4154166/150‑1 (MQ=255)
               gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                                                       >  1:3306019/1‑150 (MQ=255)
                 ttATCGTTCGCGTACTACCACCCACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCt                                                                                                                                     >  1:1327930/1‑150 (MQ=255)
                 ttATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCt                                                                                                                                     <  1:2768287/150‑1 (MQ=255)
                     cGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAg                                                                                                                                 >  1:1904078/1‑150 (MQ=255)
                        tCCAAGACTTCCACCCAAGGCCGCAACAGGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGAc                                                                                                                              <  1:795761/150‑1 (MQ=255)
                          cAAGACTTCCACCCAAGGCCGCAACATGGCCGGTGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGGTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCt                                                                                                                            <  1:2610984/150‑1 (MQ=255)
                          cAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCt                                                                                                                            <  1:1999268/150‑1 (MQ=255)
                          cAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCt                                                                                                                            >  1:584823/1‑150 (MQ=255)
                          cAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCt                                                                                                                            >  2:4876295/1‑150 (MQ=255)
                          cAAGACTTCCACCCAAGGCCGCAACATGGCCGGAGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTTCCGGGCCATGTGCACCTGAAGGACCt                                                                                                                            >  1:1386717/1‑150 (MQ=255)
                           aaGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                                                           >  2:5363679/1‑150 (MQ=255)
                           aaGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                                                           <  2:2442639/150‑1 (MQ=255)
                            aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                                                          <  2:2974227/150‑1 (MQ=255)
                            aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                                                          <  1:4469533/150‑1 (MQ=255)
                                ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                      >  1:3775550/1‑150 (MQ=255)
                                ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                      >  2:4469314/1‑150 (MQ=255)
                                ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCAACCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                      >  1:1111374/1‑150 (MQ=255)
                                 tCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAg                                                                                                                     >  1:4076361/1‑150 (MQ=255)
                                  ccACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGACACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGc                                                                                                                    >  1:646879/1‑150 (MQ=255)
                                    aCCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCtg                                                                                                                  >  2:1302480/1‑150 (MQ=255)
                                      ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                >  2:884198/1‑150 (MQ=255)
                                      ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                >  2:775419/1‑150 (MQ=255)
                                      ccAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAATAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCtggt                                                                                                                >  1:47769/1‑150 (MQ=255)
                                      ccAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCtggt                                                                                                                >  2:1021278/1‑150 (MQ=255)
                                           gCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCAt                                                                                                                                             >  2:3514319/1‑116 (MQ=255)
                                                aaCATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaa                                                                                                      >  1:4487851/1‑150 (MQ=255)
                                                   aTGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATc                                                                                                   <  2:3409641/150‑1 (MQ=255)
                                                     ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                     >  2:2124133/1‑146 (MQ=255)
                                                     ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                     <  1:2124133/146‑1 (MQ=255)
                                                      gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                <  1:1156370/150‑1 (MQ=255)
                                                      gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                >  1:1851697/1‑150 (MQ=255)
                                                        cGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAAcg                                                                                              >  1:2373720/1‑150 (MQ=255)
                                                             cccGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCGTCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCg                                                                                         <  1:2682551/150‑1 (MQ=255)
                                                                gTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtg                                                                                      >  2:3939924/1‑150 (MQ=255)
                                                                gTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCAGCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtg                                                                                      <  1:4611712/150‑1 (MQ=255)
                                                                     cTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgtg                                                                                 <  2:2115573/150‑1 (MQ=255)
                                                                         ggCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCCTGTGCACCTGAAGGACCTGGGCCCGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCa                                                                             >  2:3310718/1‑150 (MQ=255)
                                                                            gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                                                          <  2:461539/150‑1 (MQ=255)
                                                                            gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                                                          >  2:2778654/1‑150 (MQ=255)
                                                                             cgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGa                                                                         <  2:5052827/150‑1 (MQ=255)
                                                                              gcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGaa                                                                        >  2:1859869/1‑150 (MQ=255)
                                                                                 aCCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTc                                                                     <  1:882314/150‑1 (MQ=255)
                                                                                   cGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCaa                                                                   <  2:3018301/150‑1 (MQ=255)
                                                                                    gggATGAAGGAAGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAac                                                                  <  1:4383833/150‑1 (MQ=255)
                                                                                         gAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                <  1:4567169/115‑1 (MQ=255)
                                                                                           aGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATcg                                                           >  2:3807758/1‑150 (MQ=255)
                                                                                               cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGaa                                                                        <  1:1418590/133‑1 (MQ=255)
                                                                                               cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGaa                                                                        >  2:1418590/1‑133 (MQ=255)
                                                                                               cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTCCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGt                                                       <  1:4830544/150‑1 (MQ=255)
                                                                                                gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                      >  2:501734/1‑150 (MQ=255)
                                                                                                gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                      >  1:4366195/1‑150 (MQ=255)
                                                                                                  aGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGa                                                    <  2:4136356/150‑1 (MQ=255)
                                                                                                     cTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGa                                                 <  2:4590629/150‑1 (MQ=255)
                                                                                                     cTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGa                                                 <  2:1274754/150‑1 (MQ=255)
                                                                                                      ttCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGAc                                                >  2:2501513/1‑150 (MQ=255)
                                                                                                         aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                             >  2:431698/1‑150 (MQ=255)
                                                                                                         aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                             >  1:2860298/1‑150 (MQ=255)
                                                                                                         aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                             >  1:3551539/1‑150 (MQ=255)
                                                                                                         aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                             >  2:4424740/1‑150 (MQ=255)
                                                                                                            aaaCCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc                                          <  1:4002167/150‑1 (MQ=255)
                                                                                                               ccGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGcc                                       >  2:2371807/1‑150 (MQ=255)
                                                                                                                 gATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCAt                                     <  2:4679983/150‑1 (MQ=255)
                                                                                                                   tcatcGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATgg                                   <  1:3344016/150‑1 (MQ=255)
                                                                                                                     atcgccatcgccAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc                                 <  1:3076841/150‑1 (MQ=255)
                                                                                                                      tcgccatcgccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGcc                                >  1:1633461/1‑150 (MQ=255)
                                                                                                                      tcgccatcgccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGcc                                <  2:389708/150‑1 (MQ=255)
                                                                                                                            tcgccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCacgacg                          <  2:3166548/150‑1 (MQ=255)
                                                                                                                            tcgccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCacgacg                          >  2:2250593/1‑150 (MQ=255)
                                                                                                                                 aaCTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATg                     <  1:5024956/150‑1 (MQ=255)
                                                                                                                                   cTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCt                   >  1:5001597/1‑150 (MQ=255)
                                                                                                                                    tCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCtc                  >  2:2905712/1‑149 (MQ=255)
                                                                                                                                      gTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGTGTGGCCAAGGAATTc                                                                     >  2:2659105/1‑97 (MQ=255)
                                                                                                                                      gTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTa                >  1:247858/1‑150 (MQ=255)
                                                                                                                                           cccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGc           >  1:4052093/1‑150 (MQ=255)
                                                                                                                                           cccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGGGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGc           <  1:5261630/150‑1 (MQ=255)
                                                                                                                                            ccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCt          >  2:3599147/1‑150 (MQ=255)
                                                                                                                                            ccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCt          >  1:3466051/1‑150 (MQ=255)
                                                                                                                                            ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt          <  2:1523856/150‑1 (MQ=255)
                                                                                                                                            ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt          >  2:5125563/1‑150 (MQ=255)
                                                                                                                                            ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt          <  1:5263872/150‑1 (MQ=255)
                                                                                                                                            ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt          <  2:3646358/150‑1 (MQ=255)
                                                                                                                                            ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt          >  1:3911272/1‑150 (MQ=255)
                                                                                                                                            ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt          <  1:3835354/150‑1 (MQ=255)
                                                                                                                                            ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt          <  2:1734000/150‑1 (MQ=255)
                                                                                                                                                ttCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCTGCCa      <  2:4528173/150‑1 (MQ=255)
                                                                                                                                                ttCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCa      >  2:717293/1‑150 (MQ=255)
                                                                                                                                                    tACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGcc  <  1:2271223/150‑1 (MQ=255)
                                                                                                                                                    tACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGcc  <  1:456485/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAGTGTTTCATGCCTGATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCC  >  NC_002947/5851265‑5851562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑