Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,851,429 C→T 7.9% H52H (CAC→CAT)  ilvD → dihydroxy‑acid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,851,4290CT 7.9% 714.3 / 34.9 240H52H (CAC→CAT) ilvDdihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (102/119);  new base T (17/2);  total (119/121)
Fisher's exact test for biased strand distribution p-value = 2.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 251 total aligned reads displayed.

GATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGA  >  NC_002947/5851280‑5851578
                                                                                                                                                     |                                                                                                                                                     
gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                                                                       >  1:2712821/1‑150 (MQ=255)
gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                                                                       >  1:3306136/1‑150 (MQ=255)
gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                                                                       <  2:4154148/150‑1 (MQ=255)
gATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCAc                                                                                                                                                       <  2:4154172/150‑1 (MQ=255)
  ttATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCt                                                                                                                                                     <  1:2768287/150‑1 (MQ=255)
     tCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGaa                                                                                                                                                  <  2:734040/150‑1 (MQ=255)
      cGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAg                                                                                                                                                 >  1:1904078/1‑150 (MQ=255)
      cGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAg                                                                                                                                                 <  2:2950485/150‑1 (MQ=255)
         tCCAAGACTTCCACCCAAGGCCGCAACAGGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGAc                                                                                                                                              <  1:795761/150‑1 (MQ=255)
           cAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCt                                                                                                                                            >  1:584823/1‑150 (MQ=255)
           cAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCt                                                                                                                                            <  1:1999268/150‑1 (MQ=255)
            aaGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCCAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                                                                           >  1:3249215/1‑150 (MQ=255)
            aaGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                                                                           >  2:3975530/1‑150 (MQ=255)
            aaGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                                                                           >  2:5363679/1‑150 (MQ=255)
             aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCGTCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                                                                          >  2:2682551/1‑150 (MQ=255)
             aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                                                                          <  2:1771799/150‑1 (MQ=255)
             aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                                                                          <  1:4469533/150‑1 (MQ=255)
              gACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGGTCGTACCGGGCCATGTGCACCTGAAGGACCTggg                                                                                                                                         >  1:4589797/1‑150 (MQ=255)
               aCTACCACCCACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGc                                                                                                                                        <  2:2138102/150‑1 (MQ=255)
                cTTCCACCCCATGCCGCAACATGGCCGGCGCCCGTTCCCTGTGGCGCGCCACCGGGATGAAGTACGAAGTCTTCATGACACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGcc                                                                                                                                       <  2:1276495/150‑1 (MQ=255)
                 ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                                      >  2:4469314/1‑150 (MQ=255)
                 ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                                      >  1:3775550/1‑150 (MQ=255)
                 ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCAACCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                                      >  1:1111374/1‑150 (MQ=255)
                   ccACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGACACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGc                                                                                                                                    >  1:646879/1‑150 (MQ=255)
                       ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                                >  2:5349204/1‑150 (MQ=255)
                       ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                                >  2:775419/1‑150 (MQ=255)
                       ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                                >  2:884198/1‑150 (MQ=255)
                          aGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGc                                                                                                                             >  1:1374163/1‑150 (MQ=255)
                            gCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTc                                                                                                                           >  1:1556210/1‑150 (MQ=255)
                              cGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTcgc                                                                                                                         <  1:4790303/150‑1 (MQ=255)
                               gCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTcgcg                                                                                                                        >  1:3627836/1‑150 (MQ=255)
                               gCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGGGCACCTGAAGGACCTGGGCCAGCTGGTGGCTcgcg                                                                                                                        <  2:39207/150‑1 (MQ=255)
                                 aaCATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaa                                                                                                                      >  1:4487851/1‑150 (MQ=255)
                                  aCATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                                     <  2:3774303/150‑1 (MQ=255)
                                    aTGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATc                                                                                                                   <  2:3409641/150‑1 (MQ=255)
                                    aTGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATc                                                                                                                   >  2:4407549/1‑150 (MQ=255)
                                      ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                                     >  2:2124061/1‑146 (MQ=255)
                                      ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                                     <  1:2124133/146‑1 (MQ=255)
                                       gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                                <  1:1156370/150‑1 (MQ=255)
                                       gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                                <  1:3858868/150‑1 (MQ=255)
                                       gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                                >  1:1851697/1‑150 (MQ=255)
                                         cGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                                                                           <  1:463819/121‑1 (MQ=255)
                                         cGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAAcg                                                                                                              >  1:2373720/1‑150 (MQ=255)
                                             gcCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTcgc                                                                                                                         >  2:1135285/1‑135 (MQ=255)
                                                     ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgt                                                                                                  >  2:4522177/1‑150 (MQ=255)
                                                     ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgt                                                                                                  <  2:479891/150‑1 (MQ=255)
                                                      cTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgtg                                                                                                 <  2:282693/150‑1 (MQ=255)
                                                          ggCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCCTGTGCACCTGAAGGACCTGGGCCCGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCa                                                                                             >  2:3310718/1‑150 (MQ=255)
                                                           gcgcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCaa                                                                                            <  2:2566840/150‑1 (MQ=255)
                                                             gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                                                                          <  2:461539/150‑1 (MQ=255)
                                                             gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                                                                          >  2:2778654/1‑150 (MQ=255)
                                                              cgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGa                                                                                         <  2:5052827/150‑1 (MQ=255)
                                                                ccACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAAt                                                                                       <  1:5277146/150‑1 (MQ=255)
                                                                 cACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAAtt                                                                                      >  2:3825233/1‑150 (MQ=255)
                                                                    cGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCaa                                                                                   <  2:3018301/150‑1 (MQ=255)
                                                                     gggATGAAGGATGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAac                                                                                  <  1:4383812/150‑1 (MQ=255)
                                                                          gAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCAt                                                                             >  2:3108021/1‑150 (MQ=255)
                                                                                cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGaa                                                                                        <  1:1418548/133‑1 (MQ=255)
                                                                                 gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                                      >  1:4549498/1‑150 (MQ=255)
                                                                                 gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                                      <  1:2563202/150‑1 (MQ=255)
                                                                                 gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                                      >  1:5171544/1‑150 (MQ=255)
                                                                                 gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                                      >  2:501804/1‑150 (MQ=255)
                                                                                 gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                                      >  2:1431728/1‑150 (MQ=255)
                                                                                      cTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGa                                                                 <  2:4590629/150‑1 (MQ=255)
                                                                                          aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                                             >  1:2860298/1‑150 (MQ=255)
                                                                                          aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                                             >  2:431698/1‑150 (MQ=255)
                                                                                             aaaCCGATCCTCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc                                                          <  1:4002311/150‑1 (MQ=255)
                                                                                             aaaCCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc                                                          <  1:4391088/150‑1 (MQ=255)
                                                                                                ccGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGcc                                                       >  2:2371807/1‑150 (MQ=255)
                                                                                                  gATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCAt                                                     <  2:4679983/150‑1 (MQ=255)
                                                                                                    tcatcGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATgg                                                   <  1:895970/150‑1 (MQ=255)
                                                                                                      atcgccatcgccAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc                                                 <  1:3076841/150‑1 (MQ=255)
                                                                                                      atcgccatcgccAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc                                                 <  1:347385/150‑1 (MQ=255)
                                                                                                             tcgccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCacgacg                                          >  2:2250593/1‑150 (MQ=255)
                                                                                                                 gaaCTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCAt                                      >  2:1588682/2‑150 (MQ=255)
                                                                                                                  aaCTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGGGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATg                                     <  2:4925519/150‑1 (MQ=255)
                                                                                                                    cTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCt                                   >  1:5001866/1‑150 (MQ=255)
                                                                                                                     tCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCtc                                  >  2:2905712/1‑149 (MQ=255)
                                                                                                                            cccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGc                           >  1:4052093/1‑150 (MQ=255)
                                                                                                                            cccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGc                           <  1:3371943/150‑1 (MQ=255)
                                                                                                                             ccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCt                          >  2:1380313/1‑150 (MQ=255)
                                                                                                                             ccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCt                          >  2:3599147/1‑150 (MQ=255)
                                                                                                                             ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                          <  2:3646358/150‑1 (MQ=255)
                                                                                                                             ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                          <  1:5263871/150‑1 (MQ=255)
                                                                                                                             ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                          >  1:3911272/1‑150 (MQ=255)
                                                                                                                             caaGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGGGGCCACGGACTTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                          >  1:2627968/3‑150 (MQ=255)
                                                                                                                              cAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTg                         >  1:4927375/1‑150 (MQ=255)
                                                                                                                                     tACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGcc                  <  1:2271223/150‑1 (MQ=255)
                                                                                                                                     tACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGcc                  <  1:456618/150‑1 (MQ=255)
                                                                                                                                     tACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGcc                  >  2:2481413/1‑150 (MQ=255)
                                                                                                                                        cGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCcgcg               >  1:4166005/1‑150 (MQ=255)
                                                                                                                                          ggCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGaa             >  2:2108601/1‑150 (MQ=255)
                                                                                                                                          ggCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGaa             <  2:1895478/150‑1 (MQ=255)
                                                                                                                                           gCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGaaa            <  2:4999441/150‑1 (MQ=255)
                                                                                                                                               tgtgCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCAt        <  1:1269567/150‑1 (MQ=255)
                                                                                                                                                 tgCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTg      <  2:3505098/150‑1 (MQ=255)
                                                                                                                                                 tgCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTg      <  1:1721428/150‑1 (MQ=255)
                                                                                                                                                  gCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGc     <  1:4231439/150‑1 (MQ=255)
                                                                                                                                                    aCCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCg   >  1:2464588/1‑150 (MQ=255)
                                                                                                                                                     ccTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGa  <  2:3306019/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTATCGTTCCAAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGA  >  NC_002947/5851280‑5851578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑