Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,851,441 G→C 8.5% L56L (CTG→CTC)  ilvD → dihydroxy‑acid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,851,4410GC 8.5% 787.6 / 45.8 244L56L (CTG→CTC) ilvDdihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base G (104/119);  new base C (18/3);  total (122/122)
Fisher's exact test for biased strand distribution p-value = 9.22e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

AAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATA  >  NC_002947/5851292‑5851590
                                                                                                                                                     |                                                                                                                                                     
aaGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTg                                                                                                                                                       >  2:5363679/1‑150 (MQ=255)
 aGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCGTCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTgg                                                                                                                                                      >  2:2682551/1‑150 (MQ=255)
   aCTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGc                                                                                                                                                    <  1:1876671/150‑1 (MQ=255)
    cTTCCACCCCATGCCGCAACATGGCCGGCGCCCGTTCCCTGTGGCGCGCCACCGGGATGAAGTACGAAGTCTTCATGACACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGcc                                                                                                                                                   <  2:1276495/150‑1 (MQ=255)
     ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                                                  >  1:3775550/1‑150 (MQ=255)
     ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                                                  >  2:4469314/1‑150 (MQ=255)
     ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGAACCTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                                                  >  1:4178206/1‑150 (MQ=255)
     ttCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCAACCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCa                                                                                                                                                  >  1:1111374/1‑150 (MQ=255)
      tCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAg                                                                                                                                                 >  1:4076361/1‑150 (MQ=255)
       ccACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGACACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGc                                                                                                                                                >  1:646879/1‑150 (MQ=255)
           ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                                            >  2:5349204/1‑150 (MQ=255)
           ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                                            >  1:2259300/1‑150 (MQ=255)
           ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                                            >  2:775419/1‑150 (MQ=255)
           ccACGGCCGCAATATGGCTGGCGCGCGCGGCCTATGGCGCGCCACCGGCATGAAGGATGAGGATTTTGGCAAGCCAATTATTGCTGTGGTGAACTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCtcgt                                                                                                                                            >  2:884198/1‑150 (MQ=255)
           ccAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCtggt                                                                                                                                            <  2:3267202/150‑1 (MQ=255)
              aGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGc                                                                                                                                         >  1:1374163/1‑150 (MQ=255)
                gCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTc                                                                                                                                       >  1:5414235/1‑150 (MQ=255)
                   gCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGGGCACCTGAAGGACCTGGGCCAGCTGGTGGCTcgcg                                                                                                                                    <  2:39207/150‑1 (MQ=255)
                      aCATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                                                 <  2:3774303/150‑1 (MQ=255)
                        aTGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATc                                                                                                                               <  2:3409641/150‑1 (MQ=255)
                          ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                                                 <  1:2124061/146‑1 (MQ=255)
                          ggCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGaaa                                                                                                                                 >  2:2124061/1‑146 (MQ=255)
                           gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                                            <  1:1156370/150‑1 (MQ=255)
                           gCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGaa                                                                                                                            >  1:1851636/1‑150 (MQ=255)
                                   ccGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCgg                                                                                                                    <  1:2449412/150‑1 (MQ=255)
                                     gTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtg                                                                                                                  >  2:3939924/1‑150 (MQ=255)
                                     gTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCAGCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtg                                                                                                                  <  1:4611712/150‑1 (MQ=255)
                                         ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgt                                                                                                              >  2:4522177/1‑150 (MQ=255)
                                         ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgt                                                                                                              >  2:2124941/1‑150 (MQ=255)
                                         ccTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgt                                                                                                              <  2:479891/150‑1 (MQ=255)
                                          cTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGgtggcgtg                                                                                                             <  2:282693/150‑1 (MQ=255)
                                               gcgcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCaa                                                                                                        <  2:2566840/150‑1 (MQ=255)
                                                 gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                                                                                      <  2:461539/150‑1 (MQ=255)
                                                 gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                                                                                      >  2:2778654/1‑150 (MQ=255)
                                                 gcgcCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAgg                                                                                                      <  1:1586938/150‑1 (MQ=255)
                                                     cACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAAtt                                                                                                  >  2:3825233/1‑150 (MQ=255)
                                                      aCCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTc                                                                                                 <  1:4486939/150‑1 (MQ=255)
                                                        cGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGACCGCGCCGGTGGCGTGGCCAAGGAATTCaa                                                                                               <  2:5023156/150‑1 (MQ=255)
                                                              gAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCAt                                                                                         >  1:5255872/1‑150 (MQ=255)
                                                                   aCGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCgg                                                                                    >  2:3584211/1‑150 (MQ=255)
                                                                    cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGaa                                                                                                    <  1:1418590/133‑1 (MQ=255)
                                                                    cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGt                                                                                   <  2:1975859/150‑1 (MQ=255)
                                                                    cGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTCCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGt                                                                                   <  1:4830544/150‑1 (MQ=255)
                                                                     gAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTc                                                                                  >  1:4366195/1‑150 (MQ=255)
                                                                       aGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGa                                                                                <  2:4136356/150‑1 (MQ=255)
                                                                          cTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGa                                                                             <  2:4590629/150‑1 (MQ=255)
                                                                           ttCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGAc                                                                            >  2:1269567/1‑150 (MQ=255)
                                                                              aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                                                         >  1:2860298/1‑150 (MQ=255)
                                                                              aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                                                         >  2:4424740/1‑150 (MQ=255)
                                                                              aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                                                         >  2:431698/1‑150 (MQ=255)
                                                                              aaGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGc                                                                         >  1:3551539/1‑150 (MQ=255)
                                                                                 aaaCCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc                                                                      <  1:1936842/150‑1 (MQ=255)
                                                                                 aaaCCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc                                                                      >  2:297996/1‑150 (MQ=255)
                                                                                 aaaCCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc                                                                      <  1:4391088/150‑1 (MQ=255)
                                                                                 aaaCCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATc                                                                      <  1:4002167/150‑1 (MQ=255)
                                                                                     cGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCa                                                                  >  2:4968924/1‑150 (MQ=255)
                                                                                      gATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCAt                                                                 >  1:4775321/1‑150 (MQ=255)
                                                                                      gATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCAt                                                                 <  2:4679983/150‑1 (MQ=255)
                                                                                        tcatcGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATgg                                                               <  1:895970/150‑1 (MQ=255)
                                                                                        tcatcGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATgg                                                               <  1:3344016/150‑1 (MQ=255)
                                                                                          atcgccatcgccAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc                                                             <  1:3076841/150‑1 (MQ=255)
                                                                                          atcgccatcgccAAATCGTTCCCCCAGTTCGTACCGGGCCATGTGCACCTGCAGGACACGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAATGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGc                                                             <  1:746/150‑1 (MQ=255)
                                                                                                 tcgccaACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCacgacg                                                      >  2:2250593/1‑150 (MQ=255)
                                                                                                      aaCTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATg                                                 <  1:5024956/150‑1 (MQ=255)
                                                                                                        cTCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCt                                               >  1:5001597/1‑150 (MQ=255)
                                                                                                         tCGTTCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCtc                                              >  2:2905712/1‑149 (MQ=255)
                                                                                                         tCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTg                                              >  2:3054956/1‑150 (MQ=255)
                                                                                                           gTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGTGTGGCCAAGGAATTc                                                                                                 <  1:2659105/97‑1 (MQ=255)
                                                                                                           gTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTa                                            >  1:247858/1‑150 (MQ=255)
                                                                                                             tCACCCAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTatt                                          >  2:3652076/1‑148 (MQ=255)
                                                                                                              cACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTc                                         <  2:2339596/150‑1 (MQ=255)
                                                                                                               aCCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCg                                        >  1:2307805/1‑150 (MQ=255)
                                                                                                                cccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGc                                       >  1:4052093/1‑150 (MQ=255)
                                                                                                                 ccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCt                                      >  2:1380313/1‑150 (MQ=255)
                                                                                                                 ccAGTTCGTGCCGGGCCATGTGCATCTGAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCt                                      >  1:3466051/1‑150 (MQ=255)
                                                                                                                 ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                                      <  2:1734000/150‑1 (MQ=255)
                                                                                                                 ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                                      >  1:3911272/1‑150 (MQ=255)
                                                                                                                 ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                                      <  2:1523856/150‑1 (MQ=255)
                                                                                                                 ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                                      <  2:3646358/150‑1 (MQ=255)
                                                                                                                 ccAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCt                                      <  1:5263872/150‑1 (MQ=255)
                                                                                                                  cAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTg                                     >  1:4927375/1‑150 (MQ=255)
                                                                                                                     ttCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCa                                  >  2:717293/1‑150 (MQ=255)
                                                                                                                         tACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGcc                              <  1:456485/150‑1 (MQ=255)
                                                                                                                         tACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGcc                              >  2:2481413/1‑150 (MQ=255)
                                                                                                                              ggCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGaa                         <  2:1895478/150‑1 (MQ=255)
                                                                                                                               gCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGaaa                        <  2:4999457/150‑1 (MQ=255)
                                                                                                                                   tgtgCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCAt                    <  1:1269567/150‑1 (MQ=255)
                                                                                                                                   tgtgCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCAt                    >  1:2714424/1‑150 (MQ=255)
                                                                                                                                    gtgCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCAtt                   >  1:458948/1‑150 (MQ=255)
                                                                                                                                        aCCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCg               >  1:4540023/1‑150 (MQ=255)
                                                                                                                                         ccTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGa              <  2:3306136/150‑1 (MQ=255)
                                                                                                                                          cTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCCTGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGAc             >  2:1423691/1‑150 (MQ=255)
                                                                                                                                            gAAGGACCTCGGCCAGCTCGTCGCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGActc           >  1:3536614/1‑148 (MQ=255)
                                                                                                                                               ggACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAATGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGt        <  2:1111374/150‑1 (MQ=255)
                                                                                                                                               ggACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGt        <  2:4178206/150‑1 (MQ=255)
                                                                                                                                               ggACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGt        >  1:3251488/1‑150 (MQ=255)
                                                                                                                                                 aCCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTgg      >  2:3492423/1‑150 (MQ=255)
                                                                                                                                                    tGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGact   >  1:3842920/1‑148 (MQ=255)
                                                                                                                                                     gggCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATa  >  1:4451196/1‑150 (MQ=255)
                                                                                                                                                     gggCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATa  >  1:3684996/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGACTTCCACCCAAGGCCGCAACATGGCCGGCGCCCGTGCCCTGTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAACCGATCATCGCCATCGCCAACTCGTTCACCCAGTTCGTACCGGGCCATGTGCACCTGAAGGACCTGGGCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATA  >  NC_002947/5851292‑5851590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑